Rroxscaffold_3G00262760

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000622
Physical Location & Seq
Reverse (-)
56737605 .. 56742511
4907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262760.1

Sequence Viewer

Length: 426 bp
ATGGGTCGAAGATCGAAGCTTTACGAGCAAAGTATGCGAGAGCATCGACCGAAGACCAAGAAAAACCGTCGTCTTGCATTGAGGCATTGTCCGCATTCTTGTGGAACAAGTTTTTGGCCGCGGCTAGAAAAGAAGAAGAATCAAGAAGAGAATGATGGAAAGCTTGTTACACTAACGTTTTCGAGTGTGTGCAGATTTCCAATTTATGAGGCTGATTTTGGTTGGGGGAAGCCTGCATGGGTGAACATGGCTTCCATGAGAATCAGCAACCAGATAATTTTCATGGACACTAAAATGGGTGATGGAATAGAGTCATATTTTAACTTCAAGGAGGAAGACATGGCCAAGTTTGAGCTTGACTCAGAGTTCCTCGAGTTGATTTCTCCAATTGGTAATGTCAAGCAAAATCCATTTGCACGCCTTTAA

Protein Analysis

141

Amino Acids

16.57

Weight (kDa)

9.35

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 56 - 123 2.5e-13 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 121
AciI CCGC 3 cut(s) 92, 119, 121
AclI AACGTT 1 cut(s) 176
AcoI YGGCCR 2 cut(s) 116, 342
AgsI TTSAA 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AloI GAACNNNNNNTCC 2 cut(s) 236, 268
AluBI AGCT 3 cut(s) 19, 163, 355
AluI AGCT 3 cut(s) 19, 163, 355
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 2 cut(s) 116, 342
AsuHPI GGTGA 2 cut(s) 253, 311
AvaI CYCGRG 1 cut(s) 371
BalI TGGCCA 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 59, 342
BccI CCATC 2 cut(s) 149, 296
BfaI CTAG 1 cut(s) 125
BisI GCNGC 2 cut(s) 119, 122
BlsI GCNGC 2 cut(s) 120, 123
BmeT110I CYCGRG 1 cut(s) 371
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 2 cut(s) 59, 342
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BsaJI CCNNGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 375
BsgI GTGCAG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 118, 344
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 371
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 2 cut(s) 118, 344
BsoBI CYCGRG 1 cut(s) 371
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 3 cut(s) 92, 119, 121
BspANI GGCC 2 cut(s) 118, 344
BspCNI CTCAG 1 cut(s) 374
BspFNI CGCG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 234, 418
BstDEI CTNAG 1 cut(s) 361
BstDSI CCRYGG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 121
BstKTI GATC 1 cut(s) 14
BstMBI GATC 1 cut(s) 11
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 25, 34, 91
BstUI CGCG 1 cut(s) 121
BstV2I GAAGAC 2 cut(s) 59, 342
BsuRI GGCC 2 cut(s) 118, 344
BtgI CCRYGG 1 cut(s) 119
Cac8I GCNNGC 2 cut(s) 234, 418
Cfr42I CCGCGG 1 cut(s) 122
CviAII CATG 5 cut(s) 237, 247, 256, 283, 340
CviJI RGCY 9 cut(s) 19, 118, 124, 163, 212, 232, 251, 344, 355
CviKI_1 RGCY 9 cut(s) 19, 118, 124, 163, 212, 232, 251, 344, 355
DdeI CTNAG 1 cut(s) 361
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
EaeI YGGCCR 2 cut(s) 116, 342
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 371
FaeI CATG 5 cut(s) 240, 250, 259, 286, 343
FaiI YATR 8 cut(s) 35, 207, 238, 248, 257, 284, 316, 341
FatI CATG 5 cut(s) 236, 246, 255, 282, 339
Fnu4HI GCNGC 2 cut(s) 119, 122
Fsp4HI GCNGC 2 cut(s) 119, 122
FspBI CTAG 1 cut(s) 125
GluI GCNGC 2 cut(s) 119, 122
HaeIII GGCC 2 cut(s) 118, 344
Hin1II CATG 5 cut(s) 240, 250, 259, 286, 343
HindIII AAGCTT 2 cut(s) 17, 161
HinfI GANTC 4 cut(s) 139, 261, 311, 359
HphI GGTGA 2 cut(s) 253, 311
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 72
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 4 cut(s) 77, 192, 236, 416
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 34, 91
HpyF3I CTNAG 1 cut(s) 361
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 5 cut(s) 240, 250, 259, 286, 343
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 1 cut(s) 11
LpnPI CCDG 2 cut(s) 246, 284
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 166
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 6 cut(s) 21, 64, 145, 148, 158, 347
MfeI CAATTG 1 cut(s) 387
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 3 cut(s) 201, 276, 387
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 2 cut(s) 320, 353
MnlI CCTC 4 cut(s) 75, 202, 325, 380
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
MseI TTAA 2 cut(s) 321, 424
MslI CAYNNNNRTG 2 cut(s) 99, 293
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 121
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 94
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 3 cut(s) 25, 34, 91
NdeII GATC 1 cut(s) 11
NlaIII CATG 5 cut(s) 240, 250, 259, 286, 343
PaeR7I CTCGAG 1 cut(s) 371
PctI GAATGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 139, 261
PkrI GCNGC 2 cut(s) 120, 123
PleI GAGTC 2 cut(s) 319, 353
PpsI GAGTC 2 cut(s) 319, 353
Psp1406I AACGTT 1 cut(s) 176
PspXI VCTCGAGB 1 cut(s) 371
RseI CAYNNNNRTG 2 cut(s) 99, 293
SacII CCGCGG 1 cut(s) 122
SaqAI TTAA 2 cut(s) 321, 424
SatI GCNGC 2 cut(s) 119, 122
Sau3AI GATC 1 cut(s) 11
SchI GAGTC 2 cut(s) 320, 353
SetI ASST 4 cut(s) 21, 165, 179, 357
SfaNI GCATC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 371
Sfr303I CCGCGG 1 cut(s) 122
SgrBI CCGCGG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 99, 293
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 3 cut(s) 201, 276, 387
SsiI CCGC 3 cut(s) 92, 119, 121
SspMI CTAG 1 cut(s) 125
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 179
TaqI TCGA 5 cut(s) 7, 14, 46, 182, 372
TaqII GACCGA 1 cut(s) 64
TasI AATT 3 cut(s) 201, 276, 387
TauI GCSGC 2 cut(s) 121, 124
TfiI GAWTC 2 cut(s) 139, 261
Tru1I TTAA 2 cut(s) 321, 424
Tru9I TTAA 2 cut(s) 321, 424
TspDTI ATGAA 1 cut(s) 271
XhoI CTCGAG 1 cut(s) 371
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.