Rw3G025900

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000622
Physical Location & Seq
Reverse (-)
34973621 .. 34974556
936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025900.1

Sequence Viewer

Length: 936 bp
ATGAATATTGAAGTTGAATTGATATCTGAGGAGATTATCAAACCATCTTCTTCAACCCCTGATGATCTTCGCCATTACCAGCTTTCCTTCCTTGATCAGTTATCTCCTCCAGTCTATAACCCTTTGGTCCTCTTTTATGAATTCAATGACGAGACGATGCCTAGCGTTACTGAAATATCCAACCACCTTAAGAAGTCCTTAGCCGAGGTCTTAACCCTTTTCTACCCATTAGCGGGACGAATCACAGACAACAAATATGTAGATTGCAATGATGAGGGCATTCTCTATGTTGAAGCTCATGCTAAGTGTGAACTTTCTGAAGTTCTCAACAATCCAGTCCCTGGTGAATTCAGTAAGTTCATGCCATTTGAACTAGATAATATTGCTAATAAGCTTCCTCTAGGCGTCCAGCTTAACATCTTTGAATGTGGAGGATTTGCTATTGGTCACTGTATTTCTCACAAGCTAGCAGATGGATTATGTCATTTCATGTTCAGCAAAACTTGGGCTGCCACTGCCCTCGGAGATCAAGCCAAAATAGAGCCTCCGGAGTTTATTTCAGCAAAACTCTTCCCACCAAGGGATTTTACAGCCTATGATGCAGGCCCTGGCATCACAAGGAATAAAGTAGCAAAGAGGTTTGTTTTTAGTGCATCTATGATAGAGGCTCTCAGAGCAAAATATCAGAATAGCAAAGGCCTAGAAAACCAGAAACGCCCATCATGTGTTGATGCTTTGTCGGCTTTCGTATGGCGTCGATATGTGGCCAACACCAAGGATACAGGACCTGCTGAGGAGTTGTACATAGTGCTCCATTCTGTGAACCTGCGTCCAAGGTTTGACCCCTCGTTGCCGCACCATTCTTTTGGAAATCTTTATCGTGCTGCCATGACAGCTCCTTTTTTAAGTAGCGGGAAGAATGCTATGGCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

311

Amino Acids

34.86

Weight (kDa)

5.87

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 298 1.3e-60 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 796, 834
AccB7I CCANNNNNTGG 1 cut(s) 341
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 3 cut(s) 233, 854, 912
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 2 cut(s) 140, 347
AcuI CTGAAG 1 cut(s) 339
AcyI GRCGYC 2 cut(s) 405, 754
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 4 cut(s) 232, 233, 341, 580
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 7 cut(s) 11, 17, 54, 145, 293, 371, 425
AjnI CCWGG 2 cut(s) 340, 607
AluBI AGCT 6 cut(s) 82, 296, 394, 412, 466, 896
AluI AGCT 6 cut(s) 82, 296, 394, 412, 466, 896
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 1 cut(s) 146
AlwNI CAGNNNCTG 3 cut(s) 341, 608, 788
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 3 cut(s) 604, 697, 765
ApeKI GCWGC 2 cut(s) 509, 884
ApoI RAATTY 2 cut(s) 140, 347
AspS9I GGNCC 3 cut(s) 127, 605, 785
AsuHPI GGTGA 1 cut(s) 356
AsuNHI GCTAGC 1 cut(s) 466
AvaII GGWCC 2 cut(s) 127, 785
BalI TGGCCA 1 cut(s) 767
Bbv12I GWGCWC 1 cut(s) 813
BbvCI CCTCAGC 1 cut(s) 792
BbvI GCAGC 2 cut(s) 496, 871
BccI CCATC 3 cut(s) 52, 467, 727
BciT130I CCWGG 2 cut(s) 342, 609
BciVI GTATCC 1 cut(s) 772
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 5 cut(s) 162, 374, 401, 467, 701
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 2 cut(s) 796, 834
BfuI GTATCC 1 cut(s) 772
BisI GCNGC 3 cut(s) 510, 854, 885
BlsI GCNGC 3 cut(s) 511, 855, 886
Bme1390I CCNGG 2 cut(s) 342, 609
Bme18I GGWCC 2 cut(s) 127, 785
BmgT120I GGNCC 3 cut(s) 127, 605, 785
BmrFI CCNGG 2 cut(s) 342, 609
BmsI GCATC 5 cut(s) 147, 589, 621, 662, 721
BmtI GCTAGC 1 cut(s) 470
BpmI CTGGAG 1 cut(s) 93
Bpu10I CCTNAGC 2 cut(s) 199, 792
BsaHI GRCGYC 2 cut(s) 405, 754
BsaJI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 233, 341, 580
Bse1I ACTGG 2 cut(s) 110, 335
Bse3DI GCAATG 1 cut(s) 274
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 2 cut(s) 342, 609
BseDI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BseLI CCNNNNNNNGG 4 cut(s) 232, 233, 341, 580
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 3 cut(s) 18, 685, 783
BseNI ACTGG 2 cut(s) 110, 335
BseRI GAGGAG 3 cut(s) 44, 96, 809
BseXI GCAGC 2 cut(s) 496, 871
BshFI GGCC 3 cut(s) 606, 699, 767
BsiHKAI GWGCWC 1 cut(s) 813
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 2 cut(s) 249, 323
BslI CCNNNNNNNGG 4 cut(s) 232, 233, 341, 580
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 249, 323
BsmI GAATGC 2 cut(s) 279, 925
BsnI GGCC 3 cut(s) 606, 699, 767
Bsp1286I GDGCHC 1 cut(s) 813
Bsp13I TCCGGA 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 3 cut(s) 64, 94, 526
BspACI CCGC 3 cut(s) 233, 854, 912
BspANI GGCC 3 cut(s) 606, 699, 767
BspCNI CTCAG 3 cut(s) 19, 684, 784
BspEI TCCGGA 1 cut(s) 547
BspMI ACCTGC 2 cut(s) 796, 834
BspOI GCTAGC 1 cut(s) 470
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 2 cut(s) 110, 335
BssECI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BssMI GATC 3 cut(s) 64, 94, 526
BssNI GRCGYC 2 cut(s) 405, 754
BssT1I CCWWGG 3 cut(s) 578, 774, 833
Bst2UI CCWGG 2 cut(s) 342, 609
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 2 cut(s) 405, 754
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 468, 604
BstDEI CTNAG 5 cut(s) 27, 199, 303, 671, 792
BstKTI GATC 3 cut(s) 67, 97, 529
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 3 cut(s) 64, 94, 526
BstMWI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
BstNI CCWGG 2 cut(s) 342, 609
BstSCI CCNGG 2 cut(s) 340, 607
BstV1I GCAGC 2 cut(s) 496, 871
BstXI CCANNNNNNTGG 1 cut(s) 866
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 3 cut(s) 606, 699, 767
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 2 cut(s) 448, 513
BveI ACCTGC 2 cut(s) 796, 834
Cac8I GCNNGC 2 cut(s) 468, 604
CaiI CAGNNNCTG 3 cut(s) 341, 608, 788
Cfr13I GGNCC 3 cut(s) 127, 605, 785
CseI GACGC 3 cut(s) 394, 743, 818
Csp6I GTAC 1 cut(s) 802
CviAII CATG 5 cut(s) 299, 361, 490, 723, 889
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 5 cut(s) 27, 199, 303, 671, 792
DpnI GATC 3 cut(s) 66, 96, 528
DpnII GATC 3 cut(s) 64, 94, 526
EaeI YGGCCR 1 cut(s) 765
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 578, 774, 833
Eco147I AGGCCT 1 cut(s) 699
Eco32I GATATC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 127, 785
Eco57I CTGAAG 1 cut(s) 339
EcoO109I RGGNCCY 2 cut(s) 605, 785
EcoRI GAATTC 2 cut(s) 140, 347
EcoRII CCWGG 2 cut(s) 340, 607
EcoRV GATATC 1 cut(s) 24
EcoT14I CCWWGG 3 cut(s) 578, 774, 833
ErhI CCWWGG 3 cut(s) 578, 774, 833
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 5 cut(s) 302, 364, 493, 726, 892
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 2 cut(s) 249, 323
FatI CATG 5 cut(s) 298, 360, 489, 722, 888
FauI CCCGC 2 cut(s) 226, 905
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 510, 854, 885
Fsp4HI GCNGC 3 cut(s) 510, 854, 885
FspBI CTAG 5 cut(s) 162, 374, 401, 467, 701
GluI GCNGC 3 cut(s) 510, 854, 885
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 3 cut(s) 606, 699, 767
HapII CCGG 1 cut(s) 548
HgaI GACGC 3 cut(s) 394, 743, 818
Hin1I GRCGYC 2 cut(s) 405, 754
Hin1II CATG 5 cut(s) 302, 364, 493, 726, 892
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 1 cut(s) 240
HpaII CCGG 1 cut(s) 548
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 311, 823
Hpy188I TCNGA 5 cut(s) 28, 319, 524, 674, 687
Hpy188III TCNNGA 1 cut(s) 548
Hpy8I GTNNAC 2 cut(s) 311, 823
Hpy99I CGWCG 1 cut(s) 759
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4V TGCA 3 cut(s) 267, 602, 653
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
HpyF3I CTNAG 5 cut(s) 27, 199, 303, 671, 792
Hsp92I GRCGYC 2 cut(s) 405, 754
Hsp92II CATG 5 cut(s) 302, 364, 493, 726, 892
Kpn2I TCCGGA 1 cut(s) 547
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 3 cut(s) 64, 94, 526
LmnI GCTCC 2 cut(s) 816, 901
Lsp1109I GCAGC 2 cut(s) 496, 871
LweI GCATC 5 cut(s) 147, 589, 621, 662, 721
MaeI CTAG 5 cut(s) 162, 374, 401, 467, 701
MaeIII GTNAC 2 cut(s) 166, 446
MalI GATC 3 cut(s) 66, 96, 528
MboI GATC 3 cut(s) 64, 94, 526
MboII GAAGA 5 cut(s) 39, 42, 59, 562, 928
MhlI GDGCHC 1 cut(s) 813
MlsI TGGCCA 1 cut(s) 767
MluCI AATT 3 cut(s) 17, 140, 347
MluNI TGGCCA 1 cut(s) 767
MmeI TCCRAC 1 cut(s) 204
Mox20I TGGCCA 1 cut(s) 767
MroI TCCGGA 1 cut(s) 547
MscI TGGCCA 1 cut(s) 767
MseI TTAA 4 cut(s) 189, 212, 414, 905
Msp20I TGGCCA 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 2 cut(s) 342, 609
Mva1269I GAATGC 2 cut(s) 279, 925
MvaI CCWGG 2 cut(s) 342, 609
MwoI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
NdeII GATC 3 cut(s) 64, 94, 526
NheI GCTAGC 1 cut(s) 466
NlaIII CATG 5 cut(s) 302, 364, 493, 726, 892
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 446
PceI AGGCCT 1 cut(s) 699
PctI GAATGC 2 cut(s) 279, 925
PfeI GAWTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 3 cut(s) 511, 855, 886
PpuMI RGGWCCY 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 340, 607
PspGI CCWGG 2 cut(s) 340, 607
PspPI GGNCC 3 cut(s) 127, 605, 785
PspPPI RGGWCCY 1 cut(s) 785
PstNI CAGNNNCTG 3 cut(s) 341, 608, 788
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
SaqAI TTAA 4 cut(s) 189, 212, 414, 905
SatI GCNGC 3 cut(s) 510, 854, 885
Sau3AI GATC 3 cut(s) 64, 94, 526
Sau96I GGNCC 3 cut(s) 127, 605, 785
ScrFI CCNGG 2 cut(s) 342, 609
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 5 cut(s) 147, 589, 621, 662, 721
SinI GGWCC 2 cut(s) 127, 785
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 17, 140, 347
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 3 cut(s) 233, 854, 912
SspI AATATT 2 cut(s) 7, 382
SspMI CTAG 5 cut(s) 162, 374, 401, 467, 701
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 2 cut(s) 340, 607
StyI CCWWGG 3 cut(s) 578, 774, 833
TaaI ACNGT 1 cut(s) 452
TaqI TCGA 1 cut(s) 757
TasI AATT 3 cut(s) 17, 140, 347
TatI WGTACW 1 cut(s) 801
TauI GCSGC 1 cut(s) 856
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 4 cut(s) 189, 212, 414, 905
Tru9I TTAA 4 cut(s) 189, 212, 414, 905
TscAI CASTG 2 cut(s) 455, 520
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 2 cut(s) 509, 884
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 4 cut(s) 17, 153, 349, 478
TspRI CASTG 2 cut(s) 455, 520
Van91I CCANNNNNTGG 1 cut(s) 341
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 127, 785
XapI RAATTY 2 cut(s) 140, 347
XspI CTAG 5 cut(s) 162, 374, 401, 467, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.