Rw4G026810

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000622
Physical Location & Seq
Reverse (-)
53069278 .. 53069745
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026810.1

Sequence Viewer

Length: 468 bp
ATGAATATTGAAGTTGAATTGATATCTGAGGAGATTATCAAACCGTCTTCTTCAACCTCTGATGATCTTCGCCATTACCAGCTTTCCTTCCTTGATCAGTTATCTCCTCCAGTCTATAACCCTTTGGTCCTCTTTTATGAATTCAATGACGAGACGATGCCTAGCGCTACTGAAATATCCAACCACCTTAAGAAGTCCTTAGCCGAGGTCTTAACCCTTTTCTACCCATTAGCGGGACGAATCACAGACAACAAATATGTAGATTGCAATGATGAGGGCATTCCCTATGTTGAAGCTCATGTTAAGTGTGAACTTTCTGAAGTTCTCAACAATCCAGCCCCTGGTGAATTCAGTAAGTTCATGCCATTTGAACTAGATAATATTGCTAATAAGCTTCCTCTAGGCGTCCAGCTTAACATCTTTGAATGTGGAGGATTTGCTATTGGTCACTGTATTCTCACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

155

Amino Acids

17.37

Weight (kDa)

4.43

Isoelectric Point (pI)

62.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 152 1.3e-35 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 1 cut(s) 233
AcsI RAATTY 2 cut(s) 140, 347
AcuI CTGAAG 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 405
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 3 cut(s) 232, 233, 341
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 7 cut(s) 11, 17, 54, 145, 293, 371, 425
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 5 cut(s) 82, 296, 394, 412, 465
AluI AGCT 5 cut(s) 82, 296, 394, 412, 465
Alw26I GTCTC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 166
ApoI RAATTY 2 cut(s) 140, 347
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 127
AsuHPI GGTGA 1 cut(s) 356
AvaII GGWCC 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 342
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 4 cut(s) 162, 374, 401, 466
BfoI RGCGCY 1 cut(s) 168
BfrI CTTAAG 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 39
BpmI CTGGAG 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 204, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 233, 341
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 204, 340
BseLI CCNNNNNNNGG 3 cut(s) 232, 233, 341
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 2 cut(s) 44, 96
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 3 cut(s) 232, 233, 341
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 249
BsmI GAATGC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 64, 94
BspACI CCGC 1 cut(s) 233
BspCNI CTCAG 1 cut(s) 19
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 204, 340
BssMI GATC 2 cut(s) 64, 94
BssNI GRCGYC 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 45, 452
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 27, 199
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 67, 97
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 64, 94
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 448
CaiI CAGNNNCTG 1 cut(s) 341
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 394
CviAII CATG 2 cut(s) 299, 361
CviJI RGCY 7 cut(s) 82, 203, 296, 338, 394, 412, 465
CviKI_1 RGCY 7 cut(s) 82, 203, 296, 338, 394, 412, 465
DdeI CTNAG 2 cut(s) 27, 199
DpnI GATC 2 cut(s) 66, 96
DpnII GATC 2 cut(s) 64, 94
Eco32I GATATC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 127
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 339
EcoRI GAATTC 2 cut(s) 140, 347
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 2 cut(s) 302, 364
FaiI YATR 6 cut(s) 117, 138, 258, 288, 300, 362
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 249
FatI CATG 2 cut(s) 298, 360
FauI CCCGC 1 cut(s) 226
FbaI TGATCA 1 cut(s) 94
FspBI CTAG 4 cut(s) 162, 374, 401, 466
GlaI GCGC 1 cut(s) 166
GsuI CTGGAG 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 168
HgaI GACGC 1 cut(s) 394
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 2 cut(s) 302, 364
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 1 cut(s) 240
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 3 cut(s) 28, 61, 319
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 45, 452
HpyCH4V TGCA 1 cut(s) 267
HpyF3I CTNAG 2 cut(s) 27, 199
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 2 cut(s) 302, 364
HspAI GCGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 64, 94
LpnPI CCDG 6 cut(s) 92, 123, 327, 348, 354, 422
LweI GCATC 1 cut(s) 147
MaeI CTAG 4 cut(s) 162, 374, 401, 466
MaeIII GTNAC 1 cut(s) 446
MalI GATC 2 cut(s) 66, 96
MboI GATC 2 cut(s) 64, 94
MboII GAAGA 3 cut(s) 39, 42, 59
MluCI AATT 3 cut(s) 17, 140, 347
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 8 cut(s) 22, 67, 117, 140, 199, 268, 408, 425
MseI TTAA 4 cut(s) 189, 212, 303, 414
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 1 cut(s) 342
NdeII GATC 2 cut(s) 64, 94
NlaIII CATG 2 cut(s) 302, 364
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 446
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 341
SaqAI TTAA 4 cut(s) 189, 212, 303, 414
Sau3AI GATC 2 cut(s) 64, 94
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 342
SetI ASST 8 cut(s) 59, 84, 189, 210, 298, 396, 414, 467
SfaNI GCATC 1 cut(s) 147
SinI GGWCC 1 cut(s) 127
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 17, 140, 347
SsiI CCGC 1 cut(s) 233
SspI AATATT 2 cut(s) 7, 382
SspMI CTAG 4 cut(s) 162, 374, 401, 466
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 45, 452
TasI AATT 3 cut(s) 17, 140, 347
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 4 cut(s) 189, 212, 303, 414
Tru9I TTAA 4 cut(s) 189, 212, 303, 414
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 3 cut(s) 17, 153, 349
TspRI CASTG 1 cut(s) 455
Van91I CCANNNNNTGG 1 cut(s) 341
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 2 cut(s) 140, 347
XspI CTAG 4 cut(s) 162, 374, 401, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.