FvH4_4g24720

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000622
Physical Location & Seq
Reverse (-)
26757088 .. 26758380
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24720.t1

Sequence Viewer

Length: 1293 bp
ATGAAGGTTGAAGTTGAATTGATCTCTGAGGAGATTATCAAACCATCTTCTTCAACCTCTGATGATCTTCACCATTACCAGCTTTCCTTCCTTGATCAGTTATCTCCTCCTGTTTATAACCCTTTGGTCCTCTTTTATGAATTCAGTGACCAGACTATGCCTAGTGTTACTGAAATATCCAACTGCCTTAAGAAGTCCTTAGCCGAGGTCTTGACTCTTTTCTACCCATTAGCGGGTCGAATCAGAGATAACAAATATGTAGACTGCAATGATGAGGGCATTCCCTATGTTGAAGCTCATGTGAAGTGTGAACTTTCTGAAGTTCTGAACAATCCGGTCCCTGGTGAATTCAGTAAGTTCATGCCGTTTGAACTGGATAATATTGATAATAAGTTTCCTCTAGGCGTCCAGCTTAATATCTTTGAATGTGGAGGATTTGCTATTGGTCATTGCATTGCTCACAAGCTTGCAGATGGATTATCTCATTTCATGTTCAGTAAAACTTGGGCTGCCACTGCCCTCGGAGATAAGGCCAAAATAGAGCATCCAGAGTTTATTTCTGCAAAACTCTTCCCACCAAGGGATTTTACTGCCTATGATGCAGGCCTTGGCATCACAAGGAATAAAGTAGCAAAGAGGTTTGTGTTTAGTACCTCTATAATAGAGGCTCTTAGAGCAAAATATAGGGACAGTGAAGGCTTAGAAAACCAGAAACCCCCATCACGTGTTGATGCTTTGTCGGCTTTCATATGGAGCCGATATGTGGCCAACACCAAGGATACAGGACCTACTGAGAAATTGTACATGGTTCTCCATTCTGTGAACCTGCGTCCAAGGTTTGACCCCCCATTGCCACACCTTTCTTTTGGAAATCTTTATCGTGCTGCCATGACAGCTCCTTTCTTGAGTAGGGGGGAAGAACGCTATGGTCTGGTGATGAGACAGGTTCGAGCAGAGATAGACAAGATTGACAATCACTACATGAAATGTCTACAACAAGGTGATGATCACTTGAGTGAGCTTAATGAAAGTGTTGATAGTGTTATCAGCGGAGAGCTGATTACATTTAGCTTCAGTAGTTTCTGCAGATTTCCTCTGTATGATAATGATTTTGGTTGGGGAAAGCCTACGTGGGTATCTTCACCACCATTGACCTTCAAGAATCTAGTGGTGTTCATGGATAGAAAAGAGGCTGATGGAATAGAGGCATATATTAGCTTGGAGGAAGAAGTCATGGCTAAGTTTGAAACTGATATCGAGTTCTTAGCTTATGCGTCTCCAAGCGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

431

Amino Acids

48.72

Weight (kDa)

5.15

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 421 2.2e-78 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 834
AccI GTMKAC 2 cut(s) 261, 991
AciI CCGC 3 cut(s) 233, 1050, 1284
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 2 cut(s) 140, 347
AcuI CTGAAG 2 cut(s) 339, 1057
AcvI CACGTG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 2 cut(s) 652, 803
AfiI CCNNNNNNNGG 5 cut(s) 232, 233, 341, 580, 763
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 8 cut(s) 11, 17, 54, 293, 371, 425, 1159, 1247
AjnI CCWGG 1 cut(s) 340
AleI CACNNNNGTG 1 cut(s) 1014
Alw26I GTCTC 2 cut(s) 934, 1281
AoxI GGCC 3 cut(s) 531, 604, 765
ApeKI GCWGC 2 cut(s) 509, 884
ApoI RAATTY 2 cut(s) 140, 347
AspS9I GGNCC 3 cut(s) 127, 337, 785
AsuHPI GGTGA 5 cut(s) 62, 356, 946, 1013, 1134
AvaII GGWCC 3 cut(s) 127, 337, 785
BaeI ACNNNNGTAYC 2 cut(s) 1119, 1152
BalI TGGCCA 1 cut(s) 767
BbrPI CACGTG 1 cut(s) 725
BbvI GCAGC 2 cut(s) 496, 871
BccI CCATC 4 cut(s) 52, 467, 727, 1190
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 772
BclI TGATCA 2 cut(s) 94, 1006
BcoDI GTCTC 2 cut(s) 934, 1281
BfaI CTAG 3 cut(s) 162, 401, 1166
BfmI CTRYAG 1 cut(s) 1084
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 834
BfuI GTATCC 1 cut(s) 772
BisI GCNGC 2 cut(s) 510, 885
BlsI GCNGC 2 cut(s) 511, 886
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 3 cut(s) 127, 337, 785
BmgT120I GGNCC 3 cut(s) 127, 337, 785
BmiI GGNNCC 2 cut(s) 339, 755
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 4 cut(s) 553, 589, 621, 721
Bpu10I CCTNAGC 1 cut(s) 199
BpuEI CTTGAG 2 cut(s) 925, 1033
BsaAI YACGTR 2 cut(s) 725, 1131
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 233, 341, 580, 763
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 4 cut(s) 274, 448, 453, 848
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 5 cut(s) 232, 233, 341, 580, 763
BseMI GCAATG 4 cut(s) 274, 448, 453, 848
BseMII CTCAG 2 cut(s) 18, 783
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 44, 96
BseXI GCAGC 2 cut(s) 496, 871
BshFI GGCC 3 cut(s) 533, 606, 767
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 2 cut(s) 323, 701
BslI CCNNNNNNNGG 5 cut(s) 232, 233, 341, 580, 763
BsmAI GTCTC 2 cut(s) 934, 1281
BsmBI CGTCTC 1 cut(s) 1281
BsmFI GGGAC 2 cut(s) 323, 701
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 3 cut(s) 533, 606, 767
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 4 cut(s) 21, 64, 94, 1006
BspACI CCGC 3 cut(s) 233, 1050, 1284
BspANI GGCC 3 cut(s) 533, 606, 767
BspCNI CTCAG 2 cut(s) 19, 784
BspLI GGNNCC 2 cut(s) 339, 755
BspMAI CTGCAG 1 cut(s) 1088
BspMI ACCTGC 1 cut(s) 834
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 4 cut(s) 274, 448, 453, 848
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 7 cut(s) 204, 340, 520, 578, 607, 774, 833
BssMI GATC 4 cut(s) 21, 64, 94, 1006
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 4 cut(s) 578, 607, 774, 833
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 692
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 801
BstBAI YACGTR 2 cut(s) 725, 1131
BstC8I GCNNGC 2 cut(s) 468, 604
BstDEI CTNAG 7 cut(s) 27, 199, 671, 700, 792, 1239, 1264
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 4 cut(s) 24, 67, 97, 1009
BstMAI GTCTC 2 cut(s) 934, 1281
BstMBI GATC 4 cut(s) 21, 64, 94, 1006
BstMWI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 1084
BstV1I GCAGC 2 cut(s) 496, 871
BstXI CCANNNNNNTGG 1 cut(s) 1287
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 3 cut(s) 533, 606, 767
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 3 cut(s) 151, 513, 697
BveI ACCTGC 1 cut(s) 834
Cac8I GCNNGC 2 cut(s) 468, 604
Cfr13I GGNCC 3 cut(s) 127, 337, 785
CseI GACGC 3 cut(s) 394, 818, 1263
Csp6I GTAC 2 cut(s) 651, 802
CviAII CATG 8 cut(s) 299, 361, 490, 805, 889, 982, 1177, 1234
CviQI GTAC 2 cut(s) 651, 802
DdeI CTNAG 7 cut(s) 27, 199, 671, 700, 792, 1239, 1264
DpnI GATC 4 cut(s) 23, 66, 96, 1008
DpnII GATC 4 cut(s) 21, 64, 94, 1006
EaeI YGGCCR 1 cut(s) 765
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 4 cut(s) 578, 607, 774, 833
Eco147I AGGCCT 1 cut(s) 606
Eco32I GATATC 1 cut(s) 1255
Eco47I GGWCC 3 cut(s) 127, 337, 785
Eco57I CTGAAG 2 cut(s) 339, 1057
Eco72I CACGTG 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 785
EcoRI GAATTC 2 cut(s) 140, 347
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 1255
EcoT14I CCWWGG 4 cut(s) 578, 607, 774, 833
ErhI CCWWGG 4 cut(s) 578, 607, 774, 833
Esp3I CGTCTC 1 cut(s) 1281
FaeI CATG 8 cut(s) 302, 364, 493, 808, 892, 985, 1180, 1237
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 2 cut(s) 323, 701
FatI CATG 8 cut(s) 298, 360, 489, 804, 888, 981, 1176, 1233
FauI CCCGC 2 cut(s) 226, 1277
FauNDI CATATG 1 cut(s) 749
FbaI TGATCA 2 cut(s) 94, 1006
FblI GTMKAC 2 cut(s) 261, 991
Fnu4HI GCNGC 2 cut(s) 510, 885
FokI GGATG 1 cut(s) 531
Fsp4HI GCNGC 2 cut(s) 510, 885
FspBI CTAG 3 cut(s) 162, 401, 1166
GluI GCNGC 2 cut(s) 510, 885
HaeIII GGCC 3 cut(s) 533, 606, 767
HapII CCGG 1 cut(s) 335
HgaI GACGC 3 cut(s) 394, 818, 1263
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 8 cut(s) 302, 364, 493, 808, 892, 985, 1180, 1237
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 3 cut(s) 214, 240, 1162
HpaII CCGG 1 cut(s) 335
HphI GGTGA 5 cut(s) 62, 356, 946, 1013, 1134
Hpy166II GTNNAC 4 cut(s) 262, 311, 823, 992
Hpy188I TCNGA 6 cut(s) 28, 61, 245, 319, 327, 524
Hpy188III TCNNGA 4 cut(s) 211, 548, 904, 1159
Hpy8I GTNNAC 4 cut(s) 262, 311, 823, 992
HpyAV CCTTC 3 cut(s) 97, 689, 1165
HpyCH4III ACNGT 1 cut(s) 692
HpyCH4IV ACGT 2 cut(s) 724, 1130
HpyCH4V TGCA 6 cut(s) 267, 453, 470, 563, 602, 1086
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
HpyF3I CTNAG 7 cut(s) 27, 199, 671, 700, 792, 1239, 1264
HpySE526I ACGT 2 cut(s) 724, 1130
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 8 cut(s) 302, 364, 493, 808, 892, 985, 1180, 1237
Ksp22I TGATCA 2 cut(s) 94, 1006
Kzo9I GATC 4 cut(s) 21, 64, 94, 1006
LmnI GCTCC 2 cut(s) 753, 901
Lsp1109I GCAGC 2 cut(s) 496, 871
LweI GCATC 4 cut(s) 553, 589, 621, 721
MaeI CTAG 3 cut(s) 162, 401, 1166
MaeII ACGT 2 cut(s) 724, 1130
MaeIII GTNAC 2 cut(s) 146, 166
MalI GATC 4 cut(s) 23, 66, 96, 1008
MboI GATC 4 cut(s) 21, 64, 94, 1006
MboII GAAGA 7 cut(s) 39, 42, 59, 562, 929, 1131, 1238
MlsI TGGCCA 1 cut(s) 767
MluCI AATT 4 cut(s) 17, 140, 347, 797
MluNI TGGCCA 1 cut(s) 767
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 204
Mox20I TGGCCA 1 cut(s) 767
MscI TGGCCA 1 cut(s) 767
MseI TTAA 3 cut(s) 189, 414, 1023
MslI CAYNNNNRTG 1 cut(s) 1014
Msp20I TGGCCA 1 cut(s) 767
MspA1I CMGCKG 1 cut(s) 1050
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 5 cut(s) 515, 599, 674, 740, 893
NdeI CATATG 1 cut(s) 749
NdeII GATC 4 cut(s) 21, 64, 94, 1006
NlaIII CATG 8 cut(s) 302, 364, 493, 808, 892, 985, 1180, 1237
NlaIV GGNNCC 2 cut(s) 339, 755
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 146
OliI CACNNNNGTG 1 cut(s) 1014
PceI AGGCCT 1 cut(s) 606
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 240, 1162
PkrI GCNGC 2 cut(s) 511, 886
PleI GAGTC 1 cut(s) 208
PmaCI CACGTG 1 cut(s) 725
PmlI CACGTG 1 cut(s) 725
PpsI GAGTC 1 cut(s) 208
Ppu21I YACGTR 2 cut(s) 725, 1131
PpuMI RGGWCCY 1 cut(s) 785
PsiI TTATAA 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 1 cut(s) 340
PspCI CACGTG 1 cut(s) 725
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 339, 755
PspPI GGNCC 3 cut(s) 127, 337, 785
PspPPI RGGWCCY 1 cut(s) 785
PstI CTGCAG 1 cut(s) 1088
RsaI GTAC 2 cut(s) 652, 803
RsaNI GTAC 2 cut(s) 651, 802
RseI CAYNNNNRTG 1 cut(s) 1014
SaqAI TTAA 3 cut(s) 189, 414, 1023
SatI GCNGC 2 cut(s) 510, 885
Sau3AI GATC 4 cut(s) 21, 64, 94, 1006
Sau96I GGNCC 3 cut(s) 127, 337, 785
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 342
SfaNI GCATC 4 cut(s) 553, 589, 621, 721
SfcI CTRYAG 1 cut(s) 1084
SinI GGWCC 3 cut(s) 127, 337, 785
SmiMI CAYNNNNRTG 1 cut(s) 1014
SmlI CTYRAG 3 cut(s) 188, 904, 1012
SmoI CTYRAG 3 cut(s) 188, 904, 1012
Sse9I AATT 4 cut(s) 17, 140, 347, 797
SseBI AGGCCT 1 cut(s) 606
SsiI CCGC 3 cut(s) 233, 1050, 1284
SspI AATATT 1 cut(s) 382
SspMI CTAG 3 cut(s) 162, 401, 1166
StuI AGGCCT 1 cut(s) 606
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 4 cut(s) 578, 607, 774, 833
TaaI ACNGT 1 cut(s) 692
TaiI ACGT 2 cut(s) 727, 1133
TaqI TCGA 3 cut(s) 238, 949, 1257
TasI AATT 4 cut(s) 17, 140, 347, 797
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 2 cut(s) 240, 1162
Tru1I TTAA 3 cut(s) 189, 414, 1023
Tru9I TTAA 3 cut(s) 189, 414, 1023
TscAI CASTG 3 cut(s) 151, 520, 697
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 2 cut(s) 509, 884
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 8 cut(s) 17, 153, 349, 478, 736, 998, 1041, 1165
TspRI CASTG 3 cut(s) 151, 520, 697
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 3 cut(s) 127, 337, 785
XapI RAATTY 2 cut(s) 140, 347
XmiI GTMKAC 2 cut(s) 261, 991
XspI CTAG 3 cut(s) 162, 401, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.