pycom10g17180

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000622
Physical Location & Seq
Forward (+)
20378980 .. 20379663
684 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17180.1

Sequence Viewer

Length: 684 bp
ATGCTGATCTGCTGTGAACTTGCGCCCGGGGATGGATCCCCCTTGCCGCGGTCTTCTTTTGGAAATATTTATCGTATTTCCATGACTGGTCCCTTCACAATTAGTAATACTAGTTTGGATAATGATCCTTGTTATGGCATGGTTAGGCATATACGAGAAGCAATGAGCAAGATTGACAATGAGTACGTGAAGCGACTGCAAGAGGGAGATGAGCACTTGAGTTCCATTAAGAAAAATGCTGACAGTTTTACCAGAGAACACTTGGTTACATATAACTTCACTAGTTTGTGCAGATATCCTCTCTATGACAATGATTTTGGTTGGGGGAGACCTACATGGGTGGGCTTACCGGCACTCACCTTCAAGAACCTAATAGTTTTCCTCGACACCAAAGAGGCTGGTGGTGGTGGTAGTGGAATAGAGGCATACGTTAGCCTGGAAAAGCAAGTTATGGCTAAATTTGAAACTGACGTCTTCCTCCGACCAAGGCTGGGCACGAGTCGAGTTAGTTCGCCAACTTCAAGCTTAACCCGCCAACAAGCCCGATTTAGTCAGGTCACAAAGTATCACACCCAAAGCCGCCCAAGTTTAGGCTGCGAGAGACCAATCCAAACCAAACCGATGAAAATCCTCTGGTCGGGTTCGGTTTCATTACGATTGCTTCTCAAGGTTTTGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

228

Amino Acids

25.54

Weight (kDa)

9.4

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 13 - 157 1e-16 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 474
AccII CGCG 1 cut(s) 49
AciI CCGC 4 cut(s) 47, 49, 532, 580
AclWI GGATC 3 cut(s) 30, 43, 119
AcsI RAATTY 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 6 cut(s) 32, 48, 134, 491, 590, 637
AgsI TTSAA 4 cut(s) 364, 464, 522, 676
AhlI ACTAGT 2 cut(s) 110, 281
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 1 cut(s) 525
AluI AGCT 1 cut(s) 525
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 2 cut(s) 322, 595
AlwI GGATC 3 cut(s) 30, 43, 119
Ama87I CYCGRG 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 594
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 89
AsuC2I CCSGG 2 cut(s) 27, 28
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 497
BamHI GGATCC 1 cut(s) 35
BauI CACGAG 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 45, 466
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 581
BccI CCATC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 437
BcnI CCSGG 2 cut(s) 27, 28
BcoDI GTCTC 2 cut(s) 322, 595
BcuI ACTAGT 2 cut(s) 110, 281
BfaI CTAG 2 cut(s) 111, 282
BisI GCNGC 3 cut(s) 47, 580, 595
BlsI GCNGC 3 cut(s) 48, 581, 596
Bme1390I CCNGG 3 cut(s) 27, 28, 437
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 37, 91
BmrFI CCNGG 3 cut(s) 27, 28, 437
BpiI GAAGAC 2 cut(s) 45, 466
BpuEI CTTGAG 2 cut(s) 238, 650
BpuMI CCSGG 2 cut(s) 27, 28
BsaAI YACGTR 1 cut(s) 187
BsaBI GATNNNNATC 2 cut(s) 123, 626
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 2 cut(s) 322, 595
BsaJI CCNNGG 4 cut(s) 26, 27, 47, 485
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 48, 134, 491, 590, 637
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 2 cut(s) 123, 626
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 4 cut(s) 26, 27, 47, 485
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 2 cut(s) 123, 626
BseLI CCNNNNNNNGG 6 cut(s) 32, 48, 134, 491, 590, 637
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 1 cut(s) 581
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 27, 350
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 6 cut(s) 32, 48, 134, 491, 590, 637
BsmAI GTCTC 2 cut(s) 322, 595
BsmFI GGGAC 1 cut(s) 75
Bso31I GGTCTC 2 cut(s) 322, 595
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 216, 497
Bsp143I GATC 3 cut(s) 6, 35, 124
BspACI CCGC 4 cut(s) 47, 49, 532, 580
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 37, 91
BspPI GGATC 3 cut(s) 30, 43, 119
BspTNI GGTCTC 2 cut(s) 322, 595
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 4 cut(s) 26, 27, 47, 485
BssMI GATC 3 cut(s) 6, 35, 124
BssNI GRCGYC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 187
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 49
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 3 cut(s) 9, 38, 127
BstMAI GTCTC 2 cut(s) 322, 595
BstMBI GATC 3 cut(s) 6, 35, 124
BstMWI GCNNNNNNNGC 1 cut(s) 531
BstNI CCWGG 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 25, 26, 435
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 581
BstV2I GAAGAC 2 cut(s) 45, 466
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 37
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 89
Cfr42I CCGCGG 1 cut(s) 50
Cfr9I CCCGGG 1 cut(s) 26
Csp6I GTAC 1 cut(s) 184
CviAII CATG 3 cut(s) 82, 139, 336
CviJI RGCY 9 cut(s) 345, 398, 435, 455, 490, 525, 542, 579, 594
CviKI_1 RGCY 9 cut(s) 345, 398, 435, 455, 490, 525, 542, 579, 594
CviQI GTAC 1 cut(s) 184
DpnI GATC 3 cut(s) 8, 37, 126
DpnII GATC 3 cut(s) 6, 35, 124
Eco130I CCWWGG 1 cut(s) 485
Eco31I GGTCTC 2 cut(s) 322, 595
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 3 cut(s) 85, 142, 339
FaqI GGGAC 1 cut(s) 75
FatI CATG 3 cut(s) 81, 138, 335
FauI CCCGC 1 cut(s) 539
Fnu4HI GCNGC 3 cut(s) 47, 580, 595
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 47, 580, 595
FspBI CTAG 2 cut(s) 111, 282
GlaI GCGC 1 cut(s) 24
GluI GCNGC 3 cut(s) 47, 580, 595
GsaI CCCAGC 1 cut(s) 494
HapII CCGG 2 cut(s) 27, 350
HhaI GCGC 1 cut(s) 25
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 3 cut(s) 85, 142, 339
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 523
HinfI GANTC 1 cut(s) 499
HpaII CCGG 2 cut(s) 27, 350
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 103, 370
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 3 cut(s) 186, 429, 471
HpyCH4V TGCA 2 cut(s) 199, 291
HpyF10VI GCNNNNNNNGC 1 cut(s) 531
HpySE526I ACGT 3 cut(s) 186, 429, 471
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 3 cut(s) 85, 142, 339
HspAI GCGC 1 cut(s) 23
KspI CCGCGG 1 cut(s) 50
Kzo9I GATC 3 cut(s) 6, 35, 124
Lsp1109I GCAGC 1 cut(s) 581
MaeI CTAG 2 cut(s) 111, 282
MaeII ACGT 3 cut(s) 186, 429, 471
MaeIII GTNAC 2 cut(s) 265, 556
MalI GATC 3 cut(s) 8, 37, 126
MboI GATC 3 cut(s) 6, 35, 124
MboII GAAGA 2 cut(s) 45, 466
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 2 cut(s) 216, 497
MluCI AATT 2 cut(s) 99, 458
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 7 cut(s) 196, 309, 388, 392, 415, 488, 641
MseI TTAA 2 cut(s) 228, 527
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 2 cut(s) 27, 350
MspR9I CCNGG 3 cut(s) 27, 28, 437
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 531
NciI CCSGG 2 cut(s) 27, 28
NdeII GATC 3 cut(s) 6, 35, 124
NlaIII CATG 3 cut(s) 85, 142, 339
NlaIV GGNNCC 2 cut(s) 37, 91
NmuCI GTSAC 1 cut(s) 556
PkrI GCNGC 3 cut(s) 48, 581, 596
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
Ppu21I YACGTR 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 37, 91
PspPI GGNCC 1 cut(s) 89
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SacII CCGCGG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 228, 527
SatI GCNGC 3 cut(s) 47, 580, 595
Sau3AI GATC 3 cut(s) 6, 35, 124
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 3 cut(s) 27, 28, 437
SduI GDGCHC 2 cut(s) 216, 497
SetI ASST 9 cut(s) 189, 334, 362, 372, 432, 474, 527, 558, 672
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SinI GGWCC 1 cut(s) 89
SmaI CCCGGG 1 cut(s) 28
SmlI CTYRAG 2 cut(s) 217, 665
SmoI CTYRAG 2 cut(s) 217, 665
SpeI ACTAGT 2 cut(s) 110, 281
Sse9I AATT 2 cut(s) 99, 458
SsiI CCGC 4 cut(s) 47, 49, 532, 580
SspI AATATT 1 cut(s) 67
SspMI CTAG 2 cut(s) 111, 282
StyD4I CCNGG 3 cut(s) 25, 26, 435
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 3 cut(s) 189, 432, 474
TaqI TCGA 2 cut(s) 384, 502
TasI AATT 2 cut(s) 99, 458
TauI GCSGC 2 cut(s) 49, 582
Tru1I TTAA 2 cut(s) 228, 527
Tru9I TTAA 2 cut(s) 228, 527
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 1 cut(s) 594
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 2 cut(s) 638, 639
TspMI CCCGGG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XmaI CCCGGG 1 cut(s) 26
XspI CTAG 2 cut(s) 111, 282
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.