Rroxscaffold_5G00373210

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000622
Physical Location & Seq
Reverse (-)
54464397 .. 54469799
5403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373210.1

Sequence Viewer

Length: 267 bp
ATGGGAATAATAACTAGTTGTGGGAAAGACCACATAGGTGATATAGAGCTCTCCTCCTTTGATCAATTAGCTCCCCTAGTCTATAACCCTTTGGTCCTCTTCTACGAATTCAATGACAAGACAGTGCCCAACATTACTGAAATATCCAGCCACCTCAAAAAGTCCCTAGCCGAAGTCTTAACCGTTTTCTACCCATTAGCCGGACGAAACAAACACGACGGCCGGCACGTTGATTGCAATGATGAGGCCGGGCATTCCCTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

88

Amino Acids

9.75

Weight (kDa)

5.33

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 13 - 82 1.7e-13 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 112
AhlI ACTAGT 1 cut(s) 14
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 2 cut(s) 49, 71
AluI AGCT 2 cut(s) 49, 71
Alw21I GWGCWC 1 cut(s) 51
AoxI GGCC 2 cut(s) 220, 246
ApoI RAATTY 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 94
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 51
BceAI ACGGC 1 cut(s) 235
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 250
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 3 cut(s) 15, 77, 167
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 250
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpuMI CCSGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 129
BseX3I CGGCCG 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 222, 248
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 3 cut(s) 201, 223, 249
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 148
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 2 cut(s) 222, 248
Bsp1286I GDGCHC 2 cut(s) 51, 129
Bsp143I GATC 1 cut(s) 61
BspANI GGCC 2 cut(s) 222, 248
BsrDI GCAATG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 222
BssAI RCCGGY 1 cut(s) 222
BssMI GATC 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 124, 184
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 224
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMCI CGRYCG 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 129
BstZI CGGCCG 1 cut(s) 220
BsuRI GGCC 2 cut(s) 222, 248
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 94
CviJI RGCY 7 cut(s) 49, 71, 150, 170, 200, 222, 248
CviKI_1 RGCY 7 cut(s) 49, 71, 150, 170, 200, 222, 248
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 220
EagI CGGCCG 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 94
Eco52I CGGCCG 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 49
EcoICRI GAGCTC 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 51
FaiI YATR 3 cut(s) 35, 44, 84
FaqI GGGAC 1 cut(s) 148
FbaI TGATCA 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 51
FspBI CTAG 3 cut(s) 15, 77, 167
HaeIII GGCC 2 cut(s) 222, 248
HapII CCGG 3 cut(s) 201, 223, 249
HpaII CCGG 3 cut(s) 201, 223, 249
HphI GGTGA 1 cut(s) 50
Hpy99I CGWCG 1 cut(s) 221
HpyCH4III ACNGT 2 cut(s) 124, 184
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 228
KroI GCCGGC 1 cut(s) 222
KroNI GCCGGC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 4 cut(s) 160, 214, 236, 262
MaeI CTAG 3 cut(s) 15, 77, 167
MaeII ACGT 1 cut(s) 228
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 51, 129
MluCI AATT 2 cut(s) 65, 107
MnlI CCTC 4 cut(s) 64, 107, 164, 238
MroNI GCCGGC 1 cut(s) 222
MseI TTAA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 3 cut(s) 201, 223, 249
MspR9I CCNGG 1 cut(s) 250
Mva1269I GAATGC 1 cut(s) 253
NaeI GCCGGC 1 cut(s) 224
NciI CCSGG 1 cut(s) 250
NdeII GATC 1 cut(s) 61
NgoMIV GCCGGC 1 cut(s) 222
OliI CACNNNNGTG 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 36
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 179
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 2 cut(s) 51, 129
SetI ASST 6 cut(s) 40, 51, 73, 156, 231, 265
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 36
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 2 cut(s) 65, 107
SspMI CTAG 3 cut(s) 15, 77, 167
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 248
TaaI ACNGT 2 cut(s) 124, 184
TaiI ACGT 1 cut(s) 231
TasI AATT 2 cut(s) 65, 107
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TscAI CASTG 1 cut(s) 129
TspRI CASTG 1 cut(s) 129
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 1 cut(s) 107
XspI CTAG 3 cut(s) 15, 77, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.