FvH4_6g21640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000866
Physical Location & Seq
Reverse (-)
15193530 .. 15194767
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21640.t2

Sequence Viewer

Length: 222 bp
ATGGCATTCTCAAAGAATCTTCTTCTTGTTAGCCTTCTGTTTGCTGTTCTCTTCATCTCTTCTGAGGTCGTCTCAGCTCATCGTCACCTAGCTGAGACTACTACTAGTGAAAATTCAAACACTGATGTTAACGATCATGGACATGGTGGCCACGGAAATGGAGGACACGGAGGACGTGGTGGCCATGGAGCCACTGCTCATGAGACTGAAACCAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

74

Amino Acids

7.59

Weight (kDa)

6.19

Isoelectric Point (pI)

15.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRP PF07172 1 - 93 2.1e-09 Glycine rich protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 148, 181
AcsI RAATTY 1 cut(s) 112
AgsI TTSAA 1 cut(s) 117
AhlI ACTAGT 1 cut(s) 104
AjiI CACGTC 1 cut(s) 176
AluBI AGCT 2 cut(s) 77, 92
AluI AGCT 2 cut(s) 77, 92
Alw26I GTCTC 3 cut(s) 76, 89, 197
AoxI GGCC 2 cut(s) 148, 181
ApoI RAATTY 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 77
BalI TGGCCA 2 cut(s) 150, 183
BcoDI GTCTC 3 cut(s) 76, 89, 197
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 2 cut(s) 89, 105
BmgBI CACGTC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 190
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BsaJI CCNNGG 2 cut(s) 151, 184
BseDI CCNNGG 2 cut(s) 151, 184
BseMII CTCAG 3 cut(s) 54, 84, 87
BshFI GGCC 2 cut(s) 150, 183
BsmAI GTCTC 3 cut(s) 76, 89, 197
BsmBI CGTCTC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 2 cut(s) 150, 183
Bsp143I GATC 1 cut(s) 133
Bsp19I CCATGG 1 cut(s) 184
BspANI GGCC 2 cut(s) 150, 183
BspCNI CTCAG 3 cut(s) 55, 85, 86
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 151, 184
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 56, 64
BstDEI CTNAG 3 cut(s) 63, 73, 93
BstDSI CCRYGG 2 cut(s) 151, 184
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 3 cut(s) 76, 89, 197
BstMBI GATC 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 158
BsuRI GGCC 2 cut(s) 150, 183
BtgI CCRYGG 2 cut(s) 151, 184
BtrI CACGTC 1 cut(s) 176
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 120, 192
CciI TCATGA 1 cut(s) 199
CviAII CATG 4 cut(s) 137, 143, 185, 200
CviJI RGCY 6 cut(s) 33, 77, 92, 150, 183, 191
CviKI_1 RGCY 6 cut(s) 33, 77, 92, 150, 183, 191
DdeI CTNAG 3 cut(s) 63, 73, 93
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 148, 181
Eam1104I CTCTTC 2 cut(s) 56, 64
EarI CTCTTC 2 cut(s) 56, 64
Eco130I CCWWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 4 cut(s) 140, 146, 188, 203
FaiI YATR 4 cut(s) 138, 144, 186, 201
FatI CATG 4 cut(s) 136, 142, 184, 199
FspBI CTAG 2 cut(s) 89, 105
HaeIII GGCC 2 cut(s) 150, 183
Hin1II CATG 4 cut(s) 140, 146, 188, 203
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 16
HpaI GTTAAC 1 cut(s) 130
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 175
HpyF3I CTNAG 3 cut(s) 63, 73, 93
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 4 cut(s) 140, 146, 188, 203
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 188
MaeI CTAG 2 cut(s) 89, 105
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 83
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 4 cut(s) 11, 14, 43, 51
MlsI TGGCCA 2 cut(s) 150, 183
MluCI AATT 2 cut(s) 112, 217
MluNI TGGCCA 2 cut(s) 150, 183
MnlI CCTC 3 cut(s) 58, 155, 164
Mox20I TGGCCA 2 cut(s) 150, 183
MscI TGGCCA 2 cut(s) 150, 183
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 2 cut(s) 141, 156
Msp20I TGGCCA 2 cut(s) 150, 183
Mva1269I GAATGC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 184
NdeII GATC 1 cut(s) 133
NlaIII CATG 4 cut(s) 140, 146, 188, 203
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 83
PagI TCATGA 1 cut(s) 199
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 141, 156
SaqAI TTAA 1 cut(s) 129
Sau3AI GATC 1 cut(s) 133
SetI ASST 5 cut(s) 69, 79, 90, 94, 178
SmiMI CAYNNNNRTG 2 cut(s) 141, 156
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 2 cut(s) 112, 217
SspMI CTAG 2 cut(s) 89, 105
StyI CCWWGG 1 cut(s) 184
TaiI ACGT 1 cut(s) 178
TasI AATT 2 cut(s) 112, 217
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 2 cut(s) 127, 199
TseFI GTSAC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 43
TspGWI ACGGA 2 cut(s) 168, 183
TspRI CASTG 2 cut(s) 127, 199
XapI RAATTY 1 cut(s) 112
XspI CTAG 2 cut(s) 89, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.