Prupe.4G225500_v2.0.a1

Glycine-Rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000866
Physical Location & Seq
Forward (+)
14230745 .. 14231513
769 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G225500.1

Sequence Viewer

Length: 273 bp
ATGGCATCCTCAAAGGCTTTTCTTCTTCTTGGTCTTGTCTTTGTTGTTCTCCTCATCTCCTCTGAGGTCTCAGCTACTCCAACTCAGGAGAGTATAGTCAACGGTGATGGCCACGGACATGGAGAGTATGGCCATGGACATGGAGGTTATGGACACGGACATGGAGGGCATAACCACGGACATGGAGGGTATGGCCATGGACATGGAGGGTATGGCCACGGCCACCACGGCAAAGGGGGGCCGGGTGCTGCTGAGACTGAAATCGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRP

Protein Analysis

91

Amino Acids

9.01

Weight (kDa)

6.23

Isoelectric Point (pI)

13.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 5 cut(s) 109, 130, 193, 214, 220
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 2 cut(s) 73, 248
AoxI GGCC 6 cut(s) 109, 130, 193, 214, 220, 239
ApeKI GCWGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 239
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 116
BalI TGGCCA 4 cut(s) 111, 132, 195, 216
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 235, 244
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 2 cut(s) 73, 248
BglI GCCNNNNNGGC 1 cut(s) 228
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 6 cut(s) 112, 133, 175, 196, 217, 226
BseDI CCNNGG 6 cut(s) 112, 133, 175, 196, 217, 226
BseGI GGATG 1 cut(s) 5
BseMII CTCAG 4 cut(s) 54, 84, 98, 243
BseRI GAGGAG 2 cut(s) 41, 49
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 6 cut(s) 111, 132, 195, 216, 222, 241
BsiSI CCGG 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 73, 248
BsnI GGCC 6 cut(s) 111, 132, 195, 216, 222, 241
Bso31I GGTCTC 1 cut(s) 73
Bsp19I CCATGG 2 cut(s) 133, 196
BspANI GGCC 6 cut(s) 111, 132, 195, 216, 222, 241
BspCNI CTCAG 4 cut(s) 55, 83, 97, 244
BspLI GGNNCC 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 73
BssECI CCNNGG 6 cut(s) 112, 133, 175, 196, 217, 226
BssT1I CCWWGG 2 cut(s) 133, 196
Bst4CI ACNGT 1 cut(s) 104
BstDEI CTNAG 4 cut(s) 63, 70, 84, 252
BstDSI CCRYGG 6 cut(s) 112, 133, 175, 196, 217, 226
BstF5I GGATG 1 cut(s) 5
BstMAI GTCTC 2 cut(s) 73, 248
BstMWI GCNNNNNNNGC 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 235
BstXI CCANNNNNNTGG 4 cut(s) 119, 140, 182, 203
BsuRI GGCC 6 cut(s) 111, 132, 195, 216, 222, 241
BtgI CCRYGG 6 cut(s) 112, 133, 175, 196, 217, 226
BtsCI GGATG 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 239
CviAII CATG 7 cut(s) 119, 134, 140, 161, 182, 197, 203
CviJI RGCY 8 cut(s) 17, 74, 111, 132, 195, 216, 222, 241
CviKI_1 RGCY 8 cut(s) 17, 74, 111, 132, 195, 216, 222, 241
DdeI CTNAG 4 cut(s) 63, 70, 84, 252
EaeI YGGCCR 5 cut(s) 109, 130, 193, 214, 220
Eco130I CCWWGG 2 cut(s) 133, 196
Eco31I GGTCTC 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 133, 196
ErhI CCWWGG 2 cut(s) 133, 196
FaeI CATG 7 cut(s) 122, 137, 143, 164, 185, 200, 206
FatI CATG 7 cut(s) 118, 133, 139, 160, 181, 196, 202
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 249
HaeIII GGCC 6 cut(s) 111, 132, 195, 216, 222, 241
HapII CCGG 1 cut(s) 242
Hin1II CATG 7 cut(s) 122, 137, 143, 164, 185, 200, 206
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HpaII CCGG 1 cut(s) 242
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 86, 265
Hpy8I GTNNAC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 1 cut(s) 228
HpyF3I CTNAG 4 cut(s) 63, 70, 84, 252
Hsp92II CATG 7 cut(s) 122, 137, 143, 164, 185, 200, 206
LpnPI CCDG 2 cut(s) 71, 255
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 1 cut(s) 14
MboII GAAGA 2 cut(s) 14, 17
MlsI TGGCCA 4 cut(s) 111, 132, 195, 216
MluCI AATT 1 cut(s) 268
MluNI TGGCCA 4 cut(s) 111, 132, 195, 216
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 8 cut(s) 19, 58, 62, 70, 137, 158, 179, 200
Mox20I TGGCCA 4 cut(s) 111, 132, 195, 216
MscI TGGCCA 4 cut(s) 111, 132, 195, 216
MslI CAYNNNNRTG 5 cut(s) 117, 138, 159, 180, 201
Msp20I TGGCCA 4 cut(s) 111, 132, 195, 216
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 228
NciI CCSGG 1 cut(s) 243
NcoI CCATGG 2 cut(s) 133, 196
NlaIII CATG 7 cut(s) 122, 137, 143, 164, 185, 200, 206
NlaIV GGNNCC 1 cut(s) 240
PkrI GCNGC 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 239
RseI CAYNNNNRTG 5 cut(s) 117, 138, 159, 180, 201
SatI GCNGC 1 cut(s) 249
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 243
SetI ASST 3 cut(s) 69, 76, 148
SfaNI GCATC 1 cut(s) 14
SmiMI CAYNNNNRTG 5 cut(s) 117, 138, 159, 180, 201
Sse9I AATT 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 2 cut(s) 133, 196
TaaI ACNGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 264
TasI AATT 1 cut(s) 268
TseI GCWGC 1 cut(s) 248
TspGWI ACGGA 3 cut(s) 129, 171, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.