Prupe.4G225600_v2.0.a1

Glycine-Rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000866
Physical Location & Seq
Forward (+)
14235750 .. 14238644
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G225600.1

Sequence Viewer

Length: 264 bp
ATGGCATCCTCAAAGGCTTTTCTTCTTCTTGGTCTTGTCTTTGCTGTTCTCCTCATCTCCTCTGAGGTCTCAGCTACTCCAACTCAGGAGAGTACAGTCAACTATGATGACAATTTTCTTGATGATCACGAGCATGGGCATGGGCATGGACATGACCATAGGCACGTCCATGGCCATGGGCATGACCACAGGCATGGACGACATGGCCATGGCCACAATGGCAAAGGGGGACCTGGTGCTGCTGAGACTGAAACCGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRP

Protein Analysis

88

Amino Acids

9.38

Weight (kDa)

6.05

Isoelectric Point (pI)

22.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 3 cut(s) 172, 205, 211
AfaI GTAC 1 cut(s) 94
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 2 cut(s) 73, 239
AoxI GGCC 3 cut(s) 172, 205, 211
ApeKI GCWGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 230
AvaII GGWCC 1 cut(s) 230
BalI TGGCCA 3 cut(s) 174, 207, 213
BauI CACGAG 1 cut(s) 128
BbvI GCAGC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 73, 239
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 3 cut(s) 169, 175, 208
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 169, 175, 208
BseGI GGATG 1 cut(s) 5
BseMII CTCAG 4 cut(s) 54, 84, 98, 234
BseRI GAGGAG 2 cut(s) 41, 49
BseXI GCAGC 1 cut(s) 226
BshFI GGCC 3 cut(s) 174, 207, 213
BslFI GGGAC 1 cut(s) 243
BsmAI GTCTC 2 cut(s) 73, 239
BsmFI GGGAC 1 cut(s) 243
BsnI GGCC 3 cut(s) 174, 207, 213
Bso31I GGTCTC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 124
Bsp19I CCATGG 3 cut(s) 169, 175, 208
BspANI GGCC 3 cut(s) 174, 207, 213
BspCNI CTCAG 4 cut(s) 55, 83, 97, 235
BspLI GGNNCC 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 169, 175, 208
BssMI GATC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 3 cut(s) 169, 175, 208
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 97
BstDEI CTNAG 4 cut(s) 63, 70, 84, 243
BstDSI CCRYGG 3 cut(s) 169, 175, 208
BstF5I GGATG 1 cut(s) 5
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 73, 239
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 226
BstXI CCANNNNNNTGG 2 cut(s) 176, 194
BsuRI GGCC 3 cut(s) 174, 207, 213
BtgI CCRYGG 3 cut(s) 169, 175, 208
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 230
CsiI ACCWGGT 1 cut(s) 232
Csp6I GTAC 1 cut(s) 93
CviJI RGCY 5 cut(s) 17, 74, 174, 207, 213
CviKI_1 RGCY 5 cut(s) 17, 74, 174, 207, 213
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 4 cut(s) 63, 70, 84, 243
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
EaeI YGGCCR 3 cut(s) 172, 205, 211
Eco130I CCWWGG 3 cut(s) 169, 175, 208
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 3 cut(s) 169, 175, 208
ErhI CCWWGG 3 cut(s) 169, 175, 208
FaqI GGGAC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 240
HaeIII GGCC 3 cut(s) 174, 207, 213
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 3 cut(s) 86, 119, 128
Hpy8I GTNNAC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 4 cut(s) 63, 70, 84, 243
HpySE526I ACGT 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 4 cut(s) 71, 175, 219, 246
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 14
MabI ACCWGGT 1 cut(s) 232
MaeII ACGT 1 cut(s) 165
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 14, 17
MlsI TGGCCA 3 cut(s) 174, 207, 213
MluCI AATT 2 cut(s) 112, 259
MluNI TGGCCA 3 cut(s) 174, 207, 213
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 4 cut(s) 19, 58, 62, 70
Mox20I TGGCCA 3 cut(s) 174, 207, 213
MscI TGGCCA 3 cut(s) 174, 207, 213
MslI CAYNNNNRTG 9 cut(s) 132, 138, 144, 150, 168, 174, 180, 192, 207
Msp20I TGGCCA 3 cut(s) 174, 207, 213
MspR9I CCNGG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 219
NcoI CCATGG 3 cut(s) 169, 175, 208
NdeII GATC 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 231
PkrI GCNGC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 230
PspPPI RGGWCCY 1 cut(s) 230
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 9 cut(s) 132, 138, 144, 150, 168, 174, 180, 192, 207
SatI GCNGC 1 cut(s) 240
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 234
SetI ASST 4 cut(s) 69, 76, 168, 235
SexAI ACCWGGT 1 cut(s) 232
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 9 cut(s) 132, 138, 144, 150, 168, 174, 180, 192, 207
Sse9I AATT 2 cut(s) 112, 259
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 3 cut(s) 169, 175, 208
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 1 cut(s) 168
TasI AATT 2 cut(s) 112, 259
TatI WGTACW 1 cut(s) 92
TseI GCWGC 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.