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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 5 |
| AciI | CCGC | 1 cut(s) | 315 |
| AclWI | GGATC | 1 cut(s) | 49 |
| AfaI | GTAC | 1 cut(s) | 91 |
| AgsI | TTSAA | 1 cut(s) | 378 |
| AluBI | AGCT | 1 cut(s) | 305 |
| AluI | AGCT | 1 cut(s) | 305 |
| Alw26I | GTCTC | 3 cut(s) | 48, 289, 302 |
| AlwI | GGATC | 1 cut(s) | 49 |
| AoxI | GGCC | 2 cut(s) | 108, 133 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| AspS9I | GGNCC | 1 cut(s) | 133 |
| AsuHPI | GGTGA | 1 cut(s) | 159 |
| BauI | CACGAG | 1 cut(s) | 324 |
| BbvCI | CCTCAGC | 1 cut(s) | 279 |
| BccI | CCATC | 1 cut(s) | 211 |
| BcoDI | GTCTC | 3 cut(s) | 48, 289, 302 |
| BfaI | CTAG | 1 cut(s) | 302 |
| BmgT120I | GGNCC | 1 cut(s) | 133 |
| Bpu10I | CCTNAGC | 1 cut(s) | 279 |
| BsaAI | YACGTR | 1 cut(s) | 89 |
| BsaI | GGTCTC | 2 cut(s) | 48, 289 |
| Bse1I | ACTGG | 1 cut(s) | 352 |
| BseMII | CTCAG | 3 cut(s) | 169, 270, 297 |
| BseNI | ACTGG | 1 cut(s) | 352 |
| BseRI | GAGGAG | 2 cut(s) | 69, 257 |
| BshFI | GGCC | 2 cut(s) | 110, 135 |
| BsmAI | GTCTC | 3 cut(s) | 48, 289, 302 |
| BsmI | GAATGC | 1 cut(s) | 221 |
| BsnI | GGCC | 2 cut(s) | 110, 135 |
| Bso31I | GGTCTC | 2 cut(s) | 48, 289 |
| Bsp143I | GATC | 3 cut(s) | 54, 95, 210 |
| BspACI | CCGC | 1 cut(s) | 315 |
| BspANI | GGCC | 2 cut(s) | 110, 135 |
| BspCNI | CTCAG | 3 cut(s) | 168, 271, 298 |
| BspPI | GGATC | 1 cut(s) | 49 |
| BspTNI | GGTCTC | 2 cut(s) | 48, 289 |
| BsrI | ACTGG | 1 cut(s) | 352 |
| BssMI | GATC | 3 cut(s) | 54, 95, 210 |
| BssSI | CACGAG | 1 cut(s) | 324 |
| Bst2BI | CACGAG | 1 cut(s) | 324 |
| Bst4CI | ACNGT | 1 cut(s) | 38 |
| Bst6I | CTCTTC | 1 cut(s) | 107 |
| BstBAI | YACGTR | 1 cut(s) | 89 |
| BstDEI | CTNAG | 3 cut(s) | 155, 279, 306 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 3 cut(s) | 57, 98, 213 |
| BstMAI | GTCTC | 3 cut(s) | 48, 289, 302 |
| BstMBI | GATC | 3 cut(s) | 54, 95, 210 |
| BsuRI | GGCC | 2 cut(s) | 110, 135 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 1 cut(s) | 133 |
| CseI | GACGC | 1 cut(s) | 129 |
| Csp6I | GTAC | 1 cut(s) | 90 |
| CviJI | RGCY | 4 cut(s) | 110, 135, 290, 305 |
| CviKI_1 | RGCY | 4 cut(s) | 110, 135, 290, 305 |
| CviQI | GTAC | 1 cut(s) | 90 |
| DdeI | CTNAG | 3 cut(s) | 155, 279, 306 |
| DpnI | GATC | 3 cut(s) | 56, 97, 212 |
| DpnII | GATC | 3 cut(s) | 54, 95, 210 |
| Eam1104I | CTCTTC | 1 cut(s) | 107 |
| EarI | CTCTTC | 1 cut(s) | 107 |
| Eco31I | GGTCTC | 2 cut(s) | 48, 289 |
| FaiI | YATR | 4 cut(s) | 122, 178, 357, 372 |
| FspBI | CTAG | 1 cut(s) | 302 |
| FspI | TGCGCA | 1 cut(s) | 5 |
| GlaI | GCGC | 1 cut(s) | 5 |
| HaeIII | GGCC | 2 cut(s) | 110, 135 |
| HgaI | GACGC | 1 cut(s) | 129 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HphI | GGTGA | 1 cut(s) | 159 |
| Hpy188I | TCNGA | 1 cut(s) | 158 |
| Hpy188III | TCNNGA | 5 cut(s) | 144, 204, 214, 296, 326 |
| HpyAV | CCTTC | 2 cut(s) | 191, 265 |
| HpyCH4III | ACNGT | 1 cut(s) | 38 |
| HpyCH4IV | ACGT | 1 cut(s) | 88 |
| HpyCH4V | TGCA | 1 cut(s) | 176 |
| HpyF3I | CTNAG | 3 cut(s) | 155, 279, 306 |
| HpySE526I | ACGT | 1 cut(s) | 88 |
| HspAI | GCGC | 1 cut(s) | 4 |
| Kzo9I | GATC | 3 cut(s) | 54, 95, 210 |
| LpnPI | CCDG | 2 cut(s) | 129, 365 |
| MaeI | CTAG | 1 cut(s) | 302 |
| MaeII | ACGT | 1 cut(s) | 88 |
| MaeIII | GTNAC | 1 cut(s) | 296 |
| MalI | GATC | 3 cut(s) | 56, 97, 212 |
| MboI | GATC | 3 cut(s) | 54, 95, 210 |
| MboII | GAAGA | 2 cut(s) | 94, 230 |
| MluCI | AATT | 4 cut(s) | 29, 50, 360, 373 |
| MnlI | CCTC | 8 cut(s) | 69, 90, 100, 124, 180, 260, 274, 278 |
| MseI | TTAA | 3 cut(s) | 32, 344, 382 |
| Mva1269I | GAATGC | 1 cut(s) | 221 |
| NdeII | GATC | 3 cut(s) | 54, 95, 210 |
| NmeAIII | GCCGAG | 1 cut(s) | 266 |
| NmuCI | GTSAC | 1 cut(s) | 296 |
| NsbI | TGCGCA | 1 cut(s) | 5 |
| PctI | GAATGC | 1 cut(s) | 221 |
| Ppu21I | YACGTR | 1 cut(s) | 89 |
| PspPI | GGNCC | 1 cut(s) | 133 |
| RsaI | GTAC | 1 cut(s) | 91 |
| RsaNI | GTAC | 1 cut(s) | 90 |
| SaqAI | TTAA | 3 cut(s) | 32, 344, 382 |
| Sau3AI | GATC | 3 cut(s) | 54, 95, 210 |
| Sau96I | GGNCC | 1 cut(s) | 133 |
| SetI | ASST | 6 cut(s) | 91, 172, 183, 285, 303, 307 |
| Sse9I | AATT | 4 cut(s) | 29, 50, 360, 373 |
| SsiI | CCGC | 1 cut(s) | 315 |
| SspMI | CTAG | 1 cut(s) | 302 |
| TaaI | ACNGT | 1 cut(s) | 38 |
| TaiI | ACGT | 1 cut(s) | 91 |
| TaqI | TCGA | 3 cut(s) | 94, 105, 213 |
| TasI | AATT | 4 cut(s) | 29, 50, 360, 373 |
| Tru1I | TTAA | 3 cut(s) | 32, 344, 382 |
| Tru9I | TTAA | 3 cut(s) | 32, 344, 382 |
| TseFI | GTSAC | 1 cut(s) | 296 |
| Tsp45I | GTSAC | 1 cut(s) | 296 |
| XspI | CTAG | 1 cut(s) | 302 |