Rorug03G0169200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000866
Physical Location & Seq
Forward (+)
14282173 .. 14284582
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0169200.1

Sequence Viewer

Length: 1173 bp
ATGGAGACCTTCCTCTTTACATCCGAATCTGTCAATGAGGGTCACCCCGACAAGCTCTGCGATCAAGTCTCGGATGCTATTCTCGATGCCTGCTTGGAACAGGACCCAGAGAGCAAAGTGGCATGTGAGACCTGTACGAAAACCAATATGGTCATGGTGTTTGGTGAAATCACAACCAAGGCTAAGGTAGACTACGAGAAGATTGTTCGAGACACATGCAGAGGAATTGGGTTTGTATCAGCTGATGTAGGCCTTGATGCTGACAAGTGCAAGGTCCTTGTCAACATTGAGGAACAAAGCCCCGAAATTGCGCAAGGGGTTCATGGCCATTTCACCAAGAAGCCTGAGGAAATCGGAGCTGGTGACCAAGGCCACATGTTTGGTTATGCCACAGATGAAACACCTGAGCTCATGCCTTTAACTCATGTCCTTTCTACTAAGCTTGGTGCCAAGCTCACTGAGGTCAGGAAGAACAAAACCGTCCCGTGGTTGAGGCCTGATGGTAAGACCCAAGTGACTGTTGAGTACAGAAATGATAACGGAGCCATGGTCCCTCTTCGGGTGCACACTATCCTTATCTCAACCCAACATGATGAGACTGTCACAAATGAGCAGATTGCTGCTGATTTGAAGGAACATGTGATCAAACCTGTTGTCCCAGCCCAGTACATTGATGACAAAACCATTTATCACCTCAACCCTTCGGGACGTTTTGTCATCGGAGGACCCCATGGAGATGCTGGCCTTACTGGTCGAAAGATCATTATAGATACCTATGGTGGTTGGGGTGCACATGGTGGTGGTGCTTTCTCGGGTAAGGATCCTACCAAGGTGGACCGAAGTGGAGCATACATTGTAAGGCAGGCAGCAAAGAGTGTGGTGGCGTCTGGCCTTGCTCGCCGATGCATTGTTCAGGTTTCTTACGCAATCGGTGTCCCTGATCCACTATCAGTGTTTGTGGATACCTACAAAACAGGCAAAATCCCAGACAAGGACATACTGGCTCTTATTAAGGAGAATTTTGACTTCAGGCCAGGAATGATTGCACTCAACCTTGATATGAAAAGGGGAGGCAACTTCAGGTATCTGAAGACTGCTGCTTATGGACACTTTGGCCGCGATGACCCAGATTTCACTTGGGAAACCGTAAAGCACCTCAAGCCAAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

390

Amino Acids

42.76

Weight (kDa)

6.02

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 4.1e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 6.7e-48 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 240 - 381 3.9e-60 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 1119
AciI CCGC 1 cut(s) 1117
AclWI GGATC 3 cut(s) 815, 828, 935
AcoI YGGCCR 2 cut(s) 325, 1114
AcsI RAATTY 1 cut(s) 1018
AcuI CTGAAG 3 cut(s) 1012, 1063, 1109
AcyI GRCGYC 1 cut(s) 884
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 3 cut(s) 136, 527, 668
AfiI CCNNNNNNNGG 3 cut(s) 486, 559, 991
AflIII ACRYGT 2 cut(s) 375, 637
AgsI TTSAA 1 cut(s) 631
AhdI GACNNNNNGTC 1 cut(s) 713
AjnI CCWGG 1 cut(s) 1033
AluBI AGCT 6 cut(s) 55, 242, 359, 409, 442, 454
AluI AGCT 6 cut(s) 55, 242, 359, 409, 442, 454
Alw21I GWGCWC 3 cut(s) 411, 567, 793
Alw26I GTCTC 4 cut(s) 73, 122, 204, 590
Alw44I GTGCAC 2 cut(s) 563, 789
AlwI GGATC 3 cut(s) 815, 828, 935
Ama87I CYCGRG 1 cut(s) 811
AoxI GGCC 8 cut(s) 250, 325, 370, 494, 742, 889, 1031, 1114
ApaLI GTGCAC 2 cut(s) 563, 789
ApeKI GCWGC 3 cut(s) 620, 866, 1097
ApoI RAATTY 1 cut(s) 1018
ArsI GACNNNNNNTTYG 2 cut(s) 639, 671
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 5 cut(s) 103, 274, 550, 725, 835
AsuHPI GGTGA 5 cut(s) 35, 176, 325, 374, 683
AvaI CYCGRG 1 cut(s) 811
AvaII GGWCC 5 cut(s) 103, 274, 550, 725, 835
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 2 cut(s) 567, 793
BalI TGGCCA 1 cut(s) 327
BamHI GGATCC 1 cut(s) 820
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 1097
Bbv12I GWGCWC 3 cut(s) 411, 567, 793
BbvI GCAGC 3 cut(s) 607, 878, 1084
BccI CCATC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BciT130I CCWGG 1 cut(s) 1035
BciVI GTATCC 1 cut(s) 955
BclI TGATCA 1 cut(s) 642
BcoDI GTCTC 4 cut(s) 73, 122, 204, 590
BfuI GTATCC 1 cut(s) 955
BisI GCNGC 4 cut(s) 621, 867, 1098, 1117
BlsI GCNGC 4 cut(s) 622, 868, 1099, 1118
Bme1390I CCNGG 1 cut(s) 1035
Bme18I GGWCC 5 cut(s) 103, 274, 550, 725, 835
BmeRI GACNNNNNGTC 1 cut(s) 713
BmeT110I CYCGRG 1 cut(s) 811
BmgT120I GGNCC 5 cut(s) 103, 274, 550, 725, 835
BmiI GGNNCC 6 cut(s) 105, 448, 544, 552, 727, 822
BmrFI CCNGG 1 cut(s) 1035
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 5 cut(s) 64, 76, 247, 727, 893
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 1097
Bpu10I CCTNAGC 2 cut(s) 183, 405
BpuEI CTTGAG 1 cut(s) 1142
BsaHI GRCGYC 1 cut(s) 884
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 6 cut(s) 177, 367, 485, 546, 730, 828
BsaXI ACNNNNNCTCC 2 cut(s) 534, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 559, 991
Bse1I ACTGG 3 cut(s) 664, 754, 1005
Bse21I CCTNAGG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 1035
BseDI CCNNGG 6 cut(s) 177, 367, 485, 546, 730, 828
BseGI GGATG 2 cut(s) 20, 79
BseLI CCNNNNNNNGG 3 cut(s) 486, 559, 991
BseMII CTCAG 3 cut(s) 336, 396, 450
BseNI ACTGG 3 cut(s) 664, 754, 1005
BseSI GKGCMC 2 cut(s) 567, 793
BseXI GCAGC 3 cut(s) 607, 878, 1084
BseYI CCCAGC 1 cut(s) 658
Bsh1236I CGCG 1 cut(s) 1119
BshFI GGCC 8 cut(s) 252, 327, 372, 496, 744, 891, 1033, 1116
BshNI GGYRCC 1 cut(s) 446
BsiHKAI GWGCWC 3 cut(s) 411, 567, 793
BsiHKCI CYCGRG 1 cut(s) 811
BslFI GGGAC 5 cut(s) 467, 536, 641, 720, 920
BslI CCNNNNNNNGG 3 cut(s) 486, 559, 991
BsmAI GTCTC 4 cut(s) 73, 122, 204, 590
BsmFI GGGAC 5 cut(s) 467, 536, 641, 720, 920
BsnI GGCC 8 cut(s) 252, 327, 372, 496, 744, 891, 1033, 1116
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 811
Bsp1286I GDGCHC 3 cut(s) 411, 567, 793
Bsp143I GATC 5 cut(s) 61, 642, 759, 820, 940
Bsp19I CCATGG 2 cut(s) 546, 730
BspACI CCGC 1 cut(s) 1117
BspANI GGCC 8 cut(s) 252, 327, 372, 496, 744, 891, 1033, 1116
BspCNI CTCAG 3 cut(s) 337, 397, 451
BspFNI CGCG 1 cut(s) 1119
BspLI GGNNCC 6 cut(s) 105, 448, 544, 552, 727, 822
BspPI GGATC 3 cut(s) 815, 828, 935
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 122
BsrI ACTGG 3 cut(s) 664, 754, 1005
BssECI CCNNGG 6 cut(s) 177, 367, 485, 546, 730, 828
BssMI GATC 5 cut(s) 61, 642, 759, 820, 940
BssNI GRCGYC 1 cut(s) 884
BssT1I CCWWGG 5 cut(s) 177, 367, 546, 730, 828
Bst2UI CCWGG 1 cut(s) 1035
Bst4CI ACNGT 4 cut(s) 481, 520, 601, 1147
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 1 cut(s) 884
BstC8I GCNNGC 4 cut(s) 91, 742, 864, 898
BstDEI CTNAG 5 cut(s) 183, 345, 405, 438, 459
BstDSI CCRYGG 3 cut(s) 485, 546, 730
BstEII GGTNACC 2 cut(s) 41, 362
BstF5I GGATG 2 cut(s) 20, 79
BstFNI CGCG 1 cut(s) 1119
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 5 cut(s) 64, 645, 762, 823, 943
BstMAI GTCTC 4 cut(s) 73, 122, 204, 590
BstMBI GATC 5 cut(s) 61, 642, 759, 820, 940
BstMWI GCNNNNNNNGC 2 cut(s) 897, 1159
BstNI CCWGG 1 cut(s) 1035
BstNSI RCATGY 4 cut(s) 126, 219, 379, 641
BstPI GGTNACC 2 cut(s) 41, 362
BstSCI CCNGG 1 cut(s) 1033
BstSLI GKGCMC 2 cut(s) 567, 793
BstUI CGCG 1 cut(s) 1119
BstV1I GCAGC 3 cut(s) 607, 878, 1084
BstV2I GAAGAC 1 cut(s) 1097
BstX2I RGATCY 1 cut(s) 820
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 1 cut(s) 820
Bsu36I CCTNAGG 1 cut(s) 345
BsuI GTATCC 1 cut(s) 955
BsuRI GGCC 8 cut(s) 252, 327, 372, 496, 744, 891, 1033, 1116
BtgI CCRYGG 3 cut(s) 485, 546, 730
BtgZI GCGATG 1 cut(s) 1134
BtsCI GGATG 2 cut(s) 20, 79
BtsIMutI CAGTG 2 cut(s) 456, 957
Cac8I GCNNGC 4 cut(s) 91, 742, 864, 898
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 5 cut(s) 103, 274, 550, 725, 835
CseI GACGC 1 cut(s) 873
Csp6I GTAC 3 cut(s) 135, 526, 667
CspCI CAANNNNNGTGG 2 cut(s) 858, 893
CviQI GTAC 3 cut(s) 135, 526, 667
DdeI CTNAG 5 cut(s) 183, 345, 405, 438, 459
DpnI GATC 5 cut(s) 63, 644, 761, 822, 942
DpnII GATC 5 cut(s) 61, 642, 759, 820, 940
DraIII CACNNNGTG 1 cut(s) 797
DriI GACNNNNNGTC 1 cut(s) 713
EaeI YGGCCR 2 cut(s) 325, 1114
Eam1104I CTCTTC 1 cut(s) 561
Eam1105I GACNNNNNGTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 5 cut(s) 177, 367, 546, 730, 828
Eco147I AGGCCT 2 cut(s) 252, 496
Eco24I GRGCYC 1 cut(s) 411
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 5 cut(s) 103, 274, 550, 725, 835
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 1012, 1063, 1109
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 811
Eco91I GGTNACC 2 cut(s) 41, 362
EcoICRI GAGCTC 1 cut(s) 409
EcoO109I RGGNCCY 3 cut(s) 103, 274, 725
EcoO65I GGTNACC 2 cut(s) 41, 362
EcoRII CCWGG 1 cut(s) 1033
EcoT14I CCWWGG 5 cut(s) 177, 367, 546, 730, 828
EcoT22I ATGCAT 1 cut(s) 908
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 5 cut(s) 177, 367, 546, 730, 828
FaqI GGGAC 5 cut(s) 467, 536, 641, 720, 920
FbaI TGATCA 1 cut(s) 642
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 4 cut(s) 621, 867, 1098, 1117
FokI GGATG 2 cut(s) 7, 86
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 4 cut(s) 621, 867, 1098, 1117
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 1 cut(s) 312
GluI GCNGC 4 cut(s) 621, 867, 1098, 1117
GsaI CCCAGC 1 cut(s) 662
HaeIII GGCC 8 cut(s) 252, 327, 372, 496, 744, 891, 1033, 1116
HgaI GACGC 1 cut(s) 873
HhaI GCGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 884
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 1 cut(s) 26
HphI GGTGA 5 cut(s) 35, 176, 325, 374, 683
Hpy166II GTNNAC 5 cut(s) 190, 283, 565, 791, 835
Hpy188I TCNGA 5 cut(s) 25, 73, 356, 722, 1089
Hpy188III TCNNGA 4 cut(s) 83, 209, 466, 705
Hpy8I GTNNAC 5 cut(s) 190, 283, 565, 791, 835
HpyAV CCTTC 3 cut(s) 19, 625, 711
HpyCH4III ACNGT 4 cut(s) 481, 520, 601, 1147
HpyCH4IV ACGT 1 cut(s) 709
HpyCH4V TGCA 6 cut(s) 219, 270, 565, 791, 906, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 897, 1159
HpyF3I CTNAG 5 cut(s) 183, 345, 405, 438, 459
HpySE526I ACGT 1 cut(s) 709
Hsp92I GRCGYC 1 cut(s) 884
HspAI GCGC 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 5 cut(s) 61, 642, 759, 820, 940
LmnI GCTCC 3 cut(s) 356, 542, 845
Lsp1109I GCAGC 3 cut(s) 607, 878, 1084
LweI GCATC 5 cut(s) 64, 76, 247, 727, 893
MaeII ACGT 1 cut(s) 709
MaeIII GTNAC 4 cut(s) 41, 362, 514, 601
MalI GATC 5 cut(s) 63, 644, 761, 822, 942
MboI GATC 5 cut(s) 61, 642, 759, 820, 940
MboII GAAGA 4 cut(s) 211, 481, 548, 1102
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 3 cut(s) 411, 567, 793
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 3 cut(s) 225, 306, 1018
MluNI TGGCCA 1 cut(s) 327
Mox20I TGGCCA 1 cut(s) 327
Mph1103I ATGCAT 1 cut(s) 908
MscI TGGCCA 1 cut(s) 327
MseI TTAA 3 cut(s) 419, 1011, 1171
MslI CAYNNNNRTG 2 cut(s) 735, 798
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 1035
MvaI CCWGG 1 cut(s) 1035
MvnI CGCG 1 cut(s) 1119
MwoI GCNNNNNNNGC 2 cut(s) 897, 1159
NcoI CCATGG 2 cut(s) 546, 730
NdeII GATC 5 cut(s) 61, 642, 759, 820, 940
NlaIV GGNNCC 6 cut(s) 105, 448, 544, 552, 727, 822
NmuCI GTSAC 4 cut(s) 41, 362, 514, 601
NsbI TGCGCA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 908
NspI RCATGY 4 cut(s) 126, 219, 379, 641
PceI AGGCCT 2 cut(s) 252, 496
PciI ACATGT 2 cut(s) 375, 637
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 4 cut(s) 622, 868, 1099, 1118
PpuMI RGGWCCY 3 cut(s) 103, 274, 725
PscI ACATGT 2 cut(s) 375, 637
Psp124BI GAGCTC 1 cut(s) 411
Psp5II RGGWCCY 3 cut(s) 103, 274, 725
Psp6I CCWGG 1 cut(s) 1033
PspEI GGTNACC 2 cut(s) 41, 362
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 1 cut(s) 1033
PspN4I GGNNCC 6 cut(s) 105, 448, 544, 552, 727, 822
PspPI GGNCC 5 cut(s) 103, 274, 550, 725, 835
PspPPI RGGWCCY 3 cut(s) 103, 274, 725
PsuI RGATCY 1 cut(s) 820
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 3 cut(s) 136, 527, 668
RsaNI GTAC 3 cut(s) 135, 526, 667
RseI CAYNNNNRTG 2 cut(s) 735, 798
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 419, 1011, 1171
SatI GCNGC 4 cut(s) 621, 867, 1098, 1117
Sau3AI GATC 5 cut(s) 61, 642, 759, 820, 940
Sau96I GGNCC 5 cut(s) 103, 274, 550, 725, 835
ScrFI CCNGG 1 cut(s) 1035
SduI GDGCHC 3 cut(s) 411, 567, 793
SfaNI GCATC 5 cut(s) 64, 76, 247, 727, 893
SinI GGWCC 5 cut(s) 103, 274, 550, 725, 835
SmiMI CAYNNNNRTG 2 cut(s) 735, 798
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
Sse9I AATT 3 cut(s) 225, 306, 1018
SseBI AGGCCT 2 cut(s) 252, 496
SsiI CCGC 1 cut(s) 1117
SstI GAGCTC 1 cut(s) 411
StuI AGGCCT 2 cut(s) 252, 496
StyD4I CCNGG 1 cut(s) 1033
StyI CCWWGG 5 cut(s) 177, 367, 546, 730, 828
TaaI ACNGT 4 cut(s) 481, 520, 601, 1147
TaiI ACGT 1 cut(s) 712
TaqI TCGA 3 cut(s) 84, 208, 754
TaqII GACCGA 1 cut(s) 852
TasI AATT 3 cut(s) 225, 306, 1018
TatI WGTACW 2 cut(s) 525, 666
TauI GCSGC 1 cut(s) 1119
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 3 cut(s) 419, 1011, 1171
Tru9I TTAA 3 cut(s) 419, 1011, 1171
TscAI CASTG 2 cut(s) 463, 957
TseFI GTSAC 4 cut(s) 41, 362, 514, 601
TseI GCWGC 3 cut(s) 620, 866, 1097
Tsp45I GTSAC 4 cut(s) 41, 362, 514, 601
TspDTI ATGAA 3 cut(s) 311, 411, 1076
TspGWI ACGGA 1 cut(s) 555
TspRI CASTG 2 cut(s) 463, 957
VneI GTGCAC 2 cut(s) 563, 789
VpaK11BI GGWCC 5 cut(s) 103, 274, 550, 725, 835
XapI RAATTY 1 cut(s) 1018
XceI RCATGY 4 cut(s) 126, 219, 379, 641
XcmI CCANNNNNNNNNTGG 3 cut(s) 151, 737, 1134
XmiI GTMKAC 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.