FvH4_6g41810

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000951
Physical Location & Seq
Forward (+)
32765178 .. 32768241
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41810.t1

Sequence Viewer

Length: 1452 bp
ATGGAAGGAGAAGGAGTGTTTTGTCACCAGTGCCGCCAAAAGACCACCAGACGTGTGTTCGTGTGCCACAACGTCATGGGGACATGCCAACTCAAGATCTGTGACCGGTGCCTCAAGAAAAGATATGATGGAAGTGTAGATGGTACTGTCATTACAGAAGAGGAGGCTGCTGCAGATGATTGGAAGTGTCCGAAATGCAGCGGGAAATGCAACTGCAGTGAGTGCATGAGGCGACAGGGTAAGGAACCTCATGGTCGAGTTAGGTCTGGCTCAAAAAAGCCTTCAGTGGAGGAGCCTGCGGAAGGGTCTGAGCCAAAGAGAGAAGGAGGTAAACAAATAGAAGACGTTGCAGCAAAGGGAAAGAGGAAGGCCAAGGCTCCTAAGGAACCCCGCAGAGTGAAGGCAAAGGAGGAAGTAGTTGAGCCTGAACTTCATCTGCCTTTAGGGGTTCCTCTAACAACTGTTGCGGGCATTGAACTACATCCTCAGGATATAGGACATGCCCTACAGTTCATACAGTTCTGCGTGGCCTTCGAAGAGGTTTTTGGATTCAGCAAAGCACAAGCTGAATCTCTTCTCAGGGACCTGGTGCTTCAACAGTACACCTCCGTATTTCAATTCCACTCCAGACTCTTATCTGTACAGGAGGTGGAATTAAAAGAAGATATCAGGCCTTTGAGCGAGGATTCCTGGTTCGAACATTTGAGGTGCTACTTCTCTGAGTCTCAACTCCTACTGAAGGAACTACCCTCCGATTTCTTTAGTAGAGGAGTTGAGGGGTACCTCTCCTTGGACTTCTCCCAGAAGCTTAGAGTCCTGAATTTCCTTTGTGATGAGGCTCTTGACACTAAAAAATTGAGCAAATTTATAGAAAGACAAGAAGGAATATTCACTGAAAGAAAGAGGGAAGCAAACCAAAAACTTTCTGCTGCAAAGGCTAAGGAACTAAGTCTCAAAGGGCAATTGAAATCTGAGGTGTCCAAAGCCATTGCTGCAGAAGAAACTGCTGCTTCATCTTCAGATCACAAAACTACTGTTTCGCGATTAAAGAGTGAAGCAGCTCAAGCTCATGCAGAATTGCTTCAAGCCAAAGCCTTGAAATCAAAATTGATAGCACATGATTCAATACGAACAGAGCCTTATCTGTTCGATGAAAATGGTCATGCATTTTGGAGATTGACAGGCTATGATGCTGGAATGGATATGTTGCTTCAAGATGTGGGAACTTGGGATCCATTTACATCTTCTGATGAGAAGTGGTTCCTGTATGAAGAAGAAAAGAAAGAAGCAGTAGAAAAGTACTGCTCTTCTGTAAGAACTAGGAAGCTTATGAATGAAAACCTGTCTCAAACACTTCCTTCAAGTTCTGGAAGCAAGGATGAGGAGAAGCTTGAAAATGCGACTACTGGAAGCGAAGAAGAAGGCATAGCCAAAAGTAGTGCAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

484

Amino Acids

54.39

Weight (kDa)

5.97

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 4 - 92 4.5e-18 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 108, 780
AccII CGCG 1 cut(s) 1042
AciI CCGC 5 cut(s) 34, 201, 299, 391, 467
AclWI GGATC 2 cut(s) 1226, 1239
AcsI RAATTY 2 cut(s) 820, 863
AcuI CTGAAG 3 cut(s) 267, 758, 1002
AfaI GTAC 5 cut(s) 145, 602, 642, 782, 1301
AfiI CCNNNNNNNGG 3 cut(s) 302, 739, 790
AflIII ACRYGT 1 cut(s) 52
AgeI ACCGGT 1 cut(s) 105
AjiI CACGTC 1 cut(s) 53
AjnI CCWGG 2 cut(s) 585, 689
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AloI GAACNNNNNNTCC 4 cut(s) 677, 709, 1216, 1248
AluBI AGCT 6 cut(s) 566, 808, 1061, 1067, 1327, 1390
AluI AGCT 6 cut(s) 566, 808, 1061, 1067, 1327, 1390
Alw26I GTCTC 3 cut(s) 729, 956, 1350
AlwI GGATC 2 cut(s) 1226, 1239
AoxI GGCC 3 cut(s) 369, 528, 671
ApeKI GCWGC 8 cut(s) 167, 170, 198, 350, 929, 992, 1007, 1058
ApoI RAATTY 2 cut(s) 820, 863
AsiGI ACCGGT 1 cut(s) 105
Asp700I GAANNNNTTC 3 cut(s) 573, 1080, 1259
Asp718I GGTACC 1 cut(s) 780
AspS9I GGNCC 1 cut(s) 583
AsuHPI GGTGA 1 cut(s) 17
AsuII TTCGAA 2 cut(s) 534, 696
AvaII GGWCC 1 cut(s) 583
AxyI CCTNAGG 2 cut(s) 381, 486
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 2 cut(s) 108, 780
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 8 cut(s) 154, 157, 210, 362, 916, 979, 994, 1070
BccI CCATC 2 cut(s) 122, 134
BciT130I CCWGG 2 cut(s) 587, 691
BcoDI GTCTC 3 cut(s) 729, 956, 1350
BfaI CTAG 1 cut(s) 1320
BfmI CTRYAG 4 cut(s) 171, 214, 506, 993
BglII AGATCT 1 cut(s) 96
BisI GCNGC 9 cut(s) 34, 168, 171, 199, 351, 930, 993, 1008, 1059
BlsI GCNGC 9 cut(s) 35, 169, 172, 200, 352, 931, 994, 1009, 1060
BmcAI AGTACT 1 cut(s) 1301
Bme1390I CCNGG 2 cut(s) 587, 691
Bme18I GGWCC 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 583
BmrFI CCNGG 2 cut(s) 587, 691
BmsI GCATC 1 cut(s) 1180
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 610
Bpu10I CCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 2 cut(s) 534, 696
BpuEI CTTGAG 3 cut(s) 77, 98, 1047
BsaJI CCNNGG 2 cut(s) 372, 789
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 739, 790
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 28, 1411
Bse21I CCTNAGG 2 cut(s) 381, 486
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 2 cut(s) 587, 691
BseDI CCNNGG 2 cut(s) 372, 789
BseGI GGATG 2 cut(s) 481, 1384
BseLI CCNNNNNNNGG 3 cut(s) 302, 739, 790
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 5 cut(s) 300, 500, 592, 711, 963
BseNI ACTGG 2 cut(s) 28, 1411
BseRI GAGGAG 4 cut(s) 176, 305, 783, 1397
BseXI GCAGC 8 cut(s) 154, 157, 210, 362, 916, 979, 994, 1070
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 3 cut(s) 371, 530, 673
BshNI GGYRCC 2 cut(s) 108, 780
BshTI ACCGGT 1 cut(s) 105
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 2 cut(s) 94, 596
BslI CCNNNNNNNGG 3 cut(s) 302, 739, 790
BsmAI GTCTC 3 cut(s) 729, 956, 1350
BsmFI GGGAC 2 cut(s) 94, 596
BsnI GGCC 3 cut(s) 371, 530, 673
Bsp119I TTCGAA 2 cut(s) 534, 696
Bsp1407I TGTACA 1 cut(s) 640
Bsp143I GATC 3 cut(s) 96, 1021, 1231
Bsp68I TCGCGA 1 cut(s) 1042
BspACI CCGC 5 cut(s) 34, 201, 299, 391, 467
BspANI GGCC 3 cut(s) 371, 530, 673
BspCNI CTCAG 5 cut(s) 301, 499, 591, 712, 964
BspFNI CGCG 1 cut(s) 1042
BspMAI CTGCAG 3 cut(s) 175, 218, 997
BspPI GGATC 2 cut(s) 1226, 1239
BspQI GCTCTTC 1 cut(s) 1312
BspT104I TTCGAA 2 cut(s) 534, 696
BspT107I GGYRCC 2 cut(s) 108, 780
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 640
BsrI ACTGG 2 cut(s) 28, 1411
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 372, 789
BssMI GATC 3 cut(s) 96, 1021, 1231
BssT1I CCWWGG 2 cut(s) 372, 789
Bst2UI CCWGG 2 cut(s) 587, 691
Bst4CI ACNGT 6 cut(s) 148, 463, 510, 519, 600, 1036
Bst6I CTCTTC 5 cut(s) 153, 531, 579, 1312, 1440
BstAPI GCANNNNNTGC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 640
BstBI TTCGAA 2 cut(s) 534, 696
BstC8I GCNNGC 2 cut(s) 297, 469
BstDEI CTNAG 9 cut(s) 309, 381, 486, 578, 720, 809, 939, 947, 972
BstENI CCTNNNNNAGG 2 cut(s) 300, 737
BstF5I GGATG 2 cut(s) 481, 1384
BstFNI CGCG 1 cut(s) 1042
BstKTI GATC 3 cut(s) 99, 1024, 1234
BstMAI GTCTC 3 cut(s) 729, 956, 1350
BstMBI GATC 3 cut(s) 96, 1021, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 207, 222, 935, 992, 1064
BstNI CCWGG 2 cut(s) 587, 691
BstNSI RCATGY 2 cut(s) 87, 503
BstSCI CCNGG 2 cut(s) 585, 689
BstSFI CTRYAG 4 cut(s) 171, 214, 506, 993
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 8 cut(s) 154, 157, 210, 362, 916, 979, 994, 1070
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 96, 1231
BstYI RGATCY 2 cut(s) 96, 1231
Bsu36I CCTNAGG 2 cut(s) 381, 486
BsuRI GGCC 3 cut(s) 371, 530, 673
BtrI CACGTC 1 cut(s) 53
BtsCI GGATG 2 cut(s) 481, 1384
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 4 cut(s) 35, 223, 291, 891
BtuMI TCGCGA 1 cut(s) 1042
Cac8I GCNNGC 2 cut(s) 297, 469
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 583
CsiI ACCWGGT 1 cut(s) 585
Csp6I GTAC 5 cut(s) 144, 601, 641, 781, 1300
CspAI ACCGGT 1 cut(s) 105
CviAII CATG 8 cut(s) 76, 84, 226, 251, 500, 1070, 1118, 1163
CviQI GTAC 5 cut(s) 144, 601, 641, 781, 1300
DdeI CTNAG 9 cut(s) 309, 381, 486, 578, 720, 809, 939, 947, 972
DpnI GATC 3 cut(s) 98, 1023, 1233
DpnII GATC 3 cut(s) 96, 1021, 1231
Eam1104I CTCTTC 5 cut(s) 153, 531, 579, 1312, 1440
EarI CTCTTC 5 cut(s) 153, 531, 579, 1312, 1440
Eco130I CCWWGG 2 cut(s) 372, 789
Eco147I AGGCCT 1 cut(s) 673
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 583
Eco57I CTGAAG 3 cut(s) 267, 758, 1002
Eco81I CCTNAGG 2 cut(s) 381, 486
EcoNI CCTNNNNNAGG 2 cut(s) 300, 737
EcoO109I RGGNCCY 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 585, 689
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 372, 789
EcoT22I ATGCAT 1 cut(s) 1168
ErhI CCWWGG 2 cut(s) 372, 789
FaeI CATG 8 cut(s) 79, 87, 229, 254, 503, 1073, 1121, 1166
FaqI GGGAC 2 cut(s) 94, 596
FatI CATG 8 cut(s) 75, 83, 225, 250, 499, 1069, 1117, 1162
FauI CCCGC 3 cut(s) 194, 398, 460
Fnu4HI GCNGC 9 cut(s) 34, 168, 171, 199, 351, 930, 993, 1008, 1059
FokI GGATG 2 cut(s) 468, 1391
Fsp4HI GCNGC 9 cut(s) 34, 168, 171, 199, 351, 930, 993, 1008, 1059
FspBI CTAG 1 cut(s) 1320
GluI GCNGC 9 cut(s) 34, 168, 171, 199, 351, 930, 993, 1008, 1059
GsuI CTGGAG 1 cut(s) 610
HaeIII GGCC 3 cut(s) 371, 530, 673
HapII CCGG 1 cut(s) 106
Hin1II CATG 8 cut(s) 79, 87, 229, 254, 503, 1073, 1121, 1166
HindIII AAGCTT 3 cut(s) 806, 1325, 1388
HinfI GANTC 7 cut(s) 549, 569, 630, 686, 722, 813, 1121
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 332, 603
Hpy188I TCNGA 7 cut(s) 192, 310, 721, 754, 973, 1021, 1249
Hpy188III TCNNGA 9 cut(s) 94, 115, 488, 627, 817, 842, 1041, 1214, 1368
Hpy8I GTNNAC 2 cut(s) 332, 603
HpyCH4III ACNGT 6 cut(s) 148, 463, 510, 519, 600, 1036
HpyCH4IV ACGT 3 cut(s) 52, 72, 345
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 222, 935, 992, 1064
HpyF3I CTNAG 9 cut(s) 309, 381, 486, 578, 720, 809, 939, 947, 972
HpySE526I ACGT 3 cut(s) 52, 72, 345
Hsp92II CATG 8 cut(s) 79, 87, 229, 254, 503, 1073, 1121, 1166
KpnI GGTACC 1 cut(s) 784
Kzo9I GATC 3 cut(s) 96, 1021, 1231
LguI GCTCTTC 1 cut(s) 1312
LmnI GCTCC 2 cut(s) 292, 382
Lsp1109I GCAGC 8 cut(s) 154, 157, 210, 362, 916, 979, 994, 1070
LweI GCATC 1 cut(s) 1180
MabI ACCWGGT 1 cut(s) 585
MaeI CTAG 1 cut(s) 1320
MaeII ACGT 3 cut(s) 52, 72, 345
MaeIII GTNAC 2 cut(s) 23, 101
MalI GATC 3 cut(s) 98, 1023, 1233
MboI GATC 3 cut(s) 96, 1021, 1231
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 2 cut(s) 96, 1231
MluCI AATT 8 cut(s) 617, 653, 820, 854, 863, 962, 1076, 1106
MlyI GAGTC 3 cut(s) 624, 731, 822
Mph1103I ATGCAT 1 cut(s) 1168
MroXI GAANNNNTTC 3 cut(s) 573, 1080, 1259
MseI TTAA 2 cut(s) 656, 1046
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 587, 691
MunI CAATTG 1 cut(s) 962
MvaI CCWGG 2 cut(s) 587, 691
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 5 cut(s) 207, 222, 935, 992, 1064
NdeII GATC 3 cut(s) 96, 1021, 1231
NlaIII CATG 8 cut(s) 79, 87, 229, 254, 503, 1073, 1121, 1166
NmuCI GTSAC 2 cut(s) 23, 101
NruI TCGCGA 1 cut(s) 1042
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 2 cut(s) 87, 503
NspV TTCGAA 2 cut(s) 534, 696
PceI AGGCCT 1 cut(s) 673
PciSI GCTCTTC 1 cut(s) 1312
PdmI GAANNNNTTC 3 cut(s) 573, 1080, 1259
PfeI GAWTC 4 cut(s) 549, 569, 686, 1121
PinAI ACCGGT 1 cut(s) 105
PkrI GCNGC 9 cut(s) 35, 169, 172, 200, 352, 931, 994, 1009, 1060
PleI GAGTC 3 cut(s) 624, 730, 821
PpsI GAGTC 3 cut(s) 624, 730, 821
PpuMI RGGWCCY 1 cut(s) 583
Psp5II RGGWCCY 1 cut(s) 583
Psp6I CCWGG 2 cut(s) 585, 689
PspGI CCWGG 2 cut(s) 585, 689
PspPI GGNCC 1 cut(s) 583
PspPPI RGGWCCY 1 cut(s) 583
PstI CTGCAG 3 cut(s) 175, 218, 997
PsuI RGATCY 2 cut(s) 96, 1231
RruI TCGCGA 1 cut(s) 1042
RsaI GTAC 5 cut(s) 145, 602, 642, 782, 1301
RsaNI GTAC 5 cut(s) 144, 601, 641, 781, 1300
SapI GCTCTTC 1 cut(s) 1312
SaqAI TTAA 2 cut(s) 656, 1046
SatI GCNGC 9 cut(s) 34, 168, 171, 199, 351, 930, 993, 1008, 1059
Sau3AI GATC 3 cut(s) 96, 1021, 1231
Sau96I GGNCC 1 cut(s) 583
ScaI AGTACT 1 cut(s) 1301
SchI GAGTC 3 cut(s) 624, 731, 822
ScrFI CCNGG 2 cut(s) 587, 691
SexAI ACCWGGT 1 cut(s) 585
SfaNI GCATC 1 cut(s) 1180
SfcI CTRYAG 4 cut(s) 171, 214, 506, 993
SfuI TTCGAA 2 cut(s) 534, 696
SinI GGWCC 1 cut(s) 583
SmlI CTYRAG 3 cut(s) 92, 113, 1062
SmoI CTYRAG 3 cut(s) 92, 113, 1062
Sse9I AATT 8 cut(s) 617, 653, 820, 854, 863, 962, 1076, 1106
SseBI AGGCCT 1 cut(s) 673
SsiI CCGC 5 cut(s) 34, 201, 299, 391, 467
SspI AATATT 1 cut(s) 888
SspMI CTAG 1 cut(s) 1320
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 2 cut(s) 585, 689
StyI CCWWGG 2 cut(s) 372, 789
TaaI ACNGT 6 cut(s) 148, 463, 510, 519, 600, 1036
TaiI ACGT 3 cut(s) 55, 75, 348
TaqI TCGA 4 cut(s) 256, 534, 696, 1149
TasI AATT 8 cut(s) 617, 653, 820, 854, 863, 962, 1076, 1106
TatI WGTACW 3 cut(s) 600, 640, 1299
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 4 cut(s) 549, 569, 686, 1121
Tru1I TTAA 2 cut(s) 656, 1046
Tru9I TTAA 2 cut(s) 656, 1046
TscAI CASTG 4 cut(s) 35, 223, 291, 898
TseFI GTSAC 2 cut(s) 23, 101
TseI GCWGC 8 cut(s) 167, 170, 198, 350, 929, 992, 1007, 1058
Tsp45I GTSAC 2 cut(s) 23, 101
TspDTI ATGAA 7 cut(s) 422, 502, 1002, 1167, 1284, 1346, 1350
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 4 cut(s) 35, 223, 291, 898
VpaK11BI GGWCC 1 cut(s) 583
XagI CCTNNNNNAGG 2 cut(s) 300, 737
XapI RAATTY 2 cut(s) 820, 863
XceI RCATGY 2 cut(s) 87, 503
XmnI GAANNNNTTC 3 cut(s) 573, 1080, 1259
XspI CTAG 1 cut(s) 1320
ZrmI AGTACT 1 cut(s) 1301
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.