Rw2G043730

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000951
Physical Location & Seq
Reverse (-)
70231150 .. 70237619
6470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043730.1

Sequence Viewer

Length: 1143 bp
ATGCAACTTCCTCCTGGTTTCGAACGAAAGGGGGAGCACAAAGTTTGTAAGTTACACAAGTCGTTATACGGATTGAAGCAAACATCTAGACAATGGTTTTTGAAGCTCTCCTCAGCTCTCCAGTCCACTGGATTTCGACAATCTTGGTCTGACTATTCCTTGTTCGTTCGAAACCATCAAGGTATCTTTACATCTTTGTTGGTTTATGTAGATGATTGCCGCCAAAGGACCACGAAACGTGTGTTCTTGTGCAACAATGTCATGGGGAAACGCCAATGCAAGATCAAGATCTGCGAGCGGAAGCGAGTAGGTAAAGAACCTCTTGGTCGAGCTGTGCCTGACTCAGAGAATCCTTCAGAGGAGATTTCGCATAGCTTATTGTCTGAGCCAAATAGAGAGGAAGAAAACCAAATAGAAGATGTTGGCAAGGGCAAGAGGAAGGCCAAGGCTCCTAGGGAACCTCGCAGAGTGAAGGCAAAGGAGGAAGTAGTTGAGCCTGAACTTCCTCTGCCTTTGGGGGTTCCCTTAACAACTGTTGCGGGCATTGAACTACATCCTCAGGATGTAGGACATGCCCTACAGTTTATAGAGTTCTGTGTGGCCCTTGAAGAGGTTCTTAAGTTCAGCAAAGAAGATGCTGAATCTCTTCTCAGATACCTAATGCATCAACGATACTCCTCAATAATCCGATTCCAGGCCAAACTCCTGTCTGAGATACAGAAGACGGGGGATGAACTGCCCTCAGATTTCTTTAGTAGAGGAGTTGACGGGTATGACTCCTTGGACTTCTCCCAGAAGCTTAGAGTCCTAACTTTCCTTTGTGATGAGGCCCTTGGCACCAAATCACTGAGCAATTTCATAGAAAGCCAAAAAGAAATATTCTCTAAAAGTAAGATCGCAAAAGCAGAGGTGGCCGAGGCCAAGGCAGTTACTGCAGGAGACAATGCTGCTTCAATTTCTTCAGATCACGAATCTACTGTTTCGCGACTGAAGAGTGAAGCAGCTCAAGATCATGCAGAGTTGCTTGAAGCAAAAGCCTTGAAATCAAGTATGTTCCCTTTTCTAGTAGTAAAATATCAAAAAACAATATTAAAAAATAGTAAAGGAAATATTAAATCCCACTTTTCAGTTTCTTCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

380

Amino Acids

42.84

Weight (kDa)

9.04

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 2 - 73 1.7e-12 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 836
AccBSI CCGCTC 1 cut(s) 298
AccII CGCG 1 cut(s) 985
AciI CCGC 3 cut(s) 220, 298, 539
AcoI YGGCCR 1 cut(s) 912
AcuI CTGAAG 3 cut(s) 339, 945, 1010
AfiI CCNNNNNNNGG 2 cut(s) 610, 694
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 7 cut(s) 76, 103, 548, 608, 954, 1028, 1042
AjnI CCWGG 2 cut(s) 13, 693
AluBI AGCT 6 cut(s) 106, 116, 332, 375, 799, 1004
AluI AGCT 6 cut(s) 106, 116, 332, 375, 799, 1004
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 933
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 6 cut(s) 441, 600, 696, 828, 912, 918
ApeKI GCWGC 2 cut(s) 947, 1001
Asp700I GAANNNNTTC 2 cut(s) 612, 645
AspA2I CCTAGG 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 228, 601, 829
AsuII TTCGAA 2 cut(s) 21, 169
AvaII GGWCC 1 cut(s) 228
AvrII CCTAGG 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 558
BanI GGYRCC 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 728
Bbv12I GWGCWC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 112
BbvI GCAGC 2 cut(s) 934, 1013
BccI CCATC 1 cut(s) 183
BciT130I CCWGG 2 cut(s) 15, 695
BcoDI GTCTC 1 cut(s) 933
BfaI CTAG 3 cut(s) 87, 453, 1064
BfmI CTRYAG 2 cut(s) 578, 933
BfrI CTTAAG 1 cut(s) 617
BglII AGATCT 1 cut(s) 288
BisI GCNGC 3 cut(s) 220, 948, 1002
BlnI CCTAGG 1 cut(s) 452
BlsI GCNGC 3 cut(s) 221, 949, 1003
Bme1390I CCNGG 2 cut(s) 15, 695
Bme18I GGWCC 1 cut(s) 228
BmgT120I GGNCC 3 cut(s) 228, 601, 829
BmiI GGNNCC 4 cut(s) 450, 459, 522, 838
BmrFI CCNGG 2 cut(s) 15, 695
BmsI GCATC 2 cut(s) 625, 673
BpiI GAAGAC 1 cut(s) 728
BpmI CTGGAG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 112
Bpu14I TTCGAA 2 cut(s) 21, 169
BpuEI CTTGAG 1 cut(s) 990
BsaBI GATNNNNATC 1 cut(s) 287
BsaJI CCNNGG 6 cut(s) 444, 452, 780, 832, 915, 921
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 694
Bse1I ACTGG 2 cut(s) 121, 133
Bse21I CCTNAGG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 287
BseBI CCWGG 2 cut(s) 15, 695
BseDI CCNNGG 6 cut(s) 444, 452, 780, 832, 915, 921
BseGI GGATG 3 cut(s) 553, 568, 736
BseJI GATNNNNATC 1 cut(s) 287
BseLI CCNNNNNNNGG 2 cut(s) 610, 694
BseMII CTCAG 8 cut(s) 126, 357, 375, 572, 664, 702, 756, 839
BseNI ACTGG 2 cut(s) 121, 133
BseRI GAGGAG 4 cut(s) 100, 374, 667, 774
BseXI GCAGC 2 cut(s) 934, 1013
Bsh1236I CGCG 1 cut(s) 985
BshFI GGCC 6 cut(s) 443, 602, 698, 830, 914, 920
BshNI GGYRCC 1 cut(s) 836
BsiHKAI GWGCWC 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 610, 694
BsmAI GTCTC 1 cut(s) 933
BsnI GGCC 6 cut(s) 443, 602, 698, 830, 914, 920
Bsp119I TTCGAA 2 cut(s) 21, 169
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 5 cut(s) 282, 288, 894, 964, 1009
Bsp68I TCGCGA 1 cut(s) 985
BspACI CCGC 3 cut(s) 220, 298, 539
BspANI GGCC 6 cut(s) 443, 602, 698, 830, 914, 920
BspCNI CTCAG 8 cut(s) 125, 356, 376, 571, 663, 703, 755, 840
BspFNI CGCG 1 cut(s) 985
BspHI TCATGA 1 cut(s) 1139
BspLI GGNNCC 4 cut(s) 450, 459, 522, 838
BspMAI CTGCAG 1 cut(s) 937
BspT104I TTCGAA 2 cut(s) 21, 169
BspT107I GGYRCC 1 cut(s) 836
BspTI CTTAAG 1 cut(s) 617
BsrBI CCGCTC 1 cut(s) 298
BsrI ACTGG 2 cut(s) 121, 133
BssECI CCNNGG 6 cut(s) 444, 452, 780, 832, 915, 921
BssMI GATC 5 cut(s) 282, 288, 894, 964, 1009
BssT1I CCWWGG 5 cut(s) 444, 452, 780, 832, 921
Bst2UI CCWGG 2 cut(s) 15, 695
Bst4CI ACNGT 3 cut(s) 535, 582, 979
Bst6I CTCTTC 3 cut(s) 603, 651, 986
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 932
BstBI TTCGAA 2 cut(s) 21, 169
BstC8I GCNNGC 2 cut(s) 296, 541
BstDEI CTNAG 9 cut(s) 112, 343, 384, 558, 650, 711, 742, 800, 848
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 3 cut(s) 553, 568, 736
BstFNI CGCG 1 cut(s) 985
BstKTI GATC 5 cut(s) 285, 291, 897, 967, 1012
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 5 cut(s) 282, 288, 894, 964, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 911, 932
BstNI CCWGG 2 cut(s) 15, 695
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 2 cut(s) 13, 693
BstSFI CTRYAG 2 cut(s) 578, 933
BstUI CGCG 1 cut(s) 985
BstV1I GCAGC 2 cut(s) 934, 1013
BstV2I GAAGAC 1 cut(s) 728
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 128
BstYI RGATCY 1 cut(s) 288
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 6 cut(s) 443, 602, 698, 830, 914, 920
BtsCI GGATG 3 cut(s) 553, 568, 736
BtsIMutI CAGTG 2 cut(s) 126, 845
BtuMI TCGCGA 1 cut(s) 985
Cac8I GCNNGC 2 cut(s) 296, 541
CaiI CAGNNNCTG 1 cut(s) 932
CciI TCATGA 1 cut(s) 1139
Cfr13I GGNCC 3 cut(s) 228, 601, 829
CviAII CATG 4 cut(s) 262, 572, 1013, 1140
DdeI CTNAG 9 cut(s) 112, 343, 384, 558, 650, 711, 742, 800, 848
DpnI GATC 5 cut(s) 284, 290, 896, 966, 1011
DpnII GATC 5 cut(s) 282, 288, 894, 964, 1009
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 3 cut(s) 603, 651, 986
EarI CTCTTC 3 cut(s) 603, 651, 986
Eco130I CCWWGG 5 cut(s) 444, 452, 780, 832, 921
Eco47I GGWCC 1 cut(s) 228
Eco57I CTGAAG 3 cut(s) 339, 945, 1010
Eco81I CCTNAGG 1 cut(s) 558
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 829
EcoRII CCWGG 2 cut(s) 13, 693
EcoT14I CCWWGG 5 cut(s) 444, 452, 780, 832, 921
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 5 cut(s) 444, 452, 780, 832, 921
FaeI CATG 4 cut(s) 265, 575, 1016, 1143
FalI AAGNNNNNCTT 4 cut(s) 306, 338, 600, 632
FatI CATG 4 cut(s) 261, 571, 1012, 1139
FauI CCCGC 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 220, 948, 1002
FokI GGATG 3 cut(s) 540, 575, 743
Fsp4HI GCNGC 3 cut(s) 220, 948, 1002
FspBI CTAG 3 cut(s) 87, 453, 1064
GluI GCNGC 3 cut(s) 220, 948, 1002
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 6 cut(s) 443, 602, 698, 830, 914, 920
Hin1II CATG 4 cut(s) 265, 575, 1016, 1143
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 7 cut(s) 341, 349, 641, 690, 776, 804, 971
Hpy166II GTNNAC 2 cut(s) 126, 766
Hpy188I TCNGA 9 cut(s) 151, 346, 358, 385, 653, 689, 712, 745, 964
Hpy188III TCNNGA 7 cut(s) 87, 286, 560, 968, 984, 1007, 1140
Hpy8I GTNNAC 2 cut(s) 126, 766
HpyAV CCTTC 3 cut(s) 363, 433, 466
HpyCH4III ACNGT 3 cut(s) 535, 582, 979
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 6 cut(s) 4, 252, 279, 664, 935, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 911, 932
HpyF3I CTNAG 9 cut(s) 112, 343, 384, 558, 650, 711, 742, 800, 848
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 4 cut(s) 265, 575, 1016, 1143
Kzo9I GATC 5 cut(s) 282, 288, 894, 964, 1009
LmnI GCTCC 2 cut(s) 34, 454
Lsp1109I GCAGC 2 cut(s) 934, 1013
LweI GCATC 2 cut(s) 625, 673
MaeI CTAG 3 cut(s) 87, 453, 1064
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 2 cut(s) 51, 928
MalI GATC 5 cut(s) 284, 290, 896, 966, 1011
MbiI CCGCTC 1 cut(s) 298
MboI GATC 5 cut(s) 282, 288, 894, 964, 1009
MboII GAAGA 9 cut(s) 413, 428, 620, 638, 644, 733, 951, 1003, 1125
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 2 cut(s) 853, 954
MlyI GAGTC 3 cut(s) 335, 770, 813
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 2 cut(s) 612, 645
MseI TTAA 4 cut(s) 527, 618, 1091, 1113
MspCI CTTAAG 1 cut(s) 617
MspR9I CCNGG 2 cut(s) 15, 695
MvaI CCWGG 2 cut(s) 15, 695
MvnI CGCG 1 cut(s) 985
MwoI GCNNNNNNNGC 2 cut(s) 911, 932
NdeII GATC 5 cut(s) 282, 288, 894, 964, 1009
NlaIII CATG 4 cut(s) 265, 575, 1016, 1143
NlaIV GGNNCC 4 cut(s) 450, 459, 522, 838
NmeAIII GCCGAG 1 cut(s) 940
NruI TCGCGA 1 cut(s) 985
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 1 cut(s) 575
NspV TTCGAA 2 cut(s) 21, 169
PagI TCATGA 1 cut(s) 1139
PdmI GAANNNNTTC 2 cut(s) 612, 645
PfeI GAWTC 4 cut(s) 349, 641, 690, 971
PkrI GCNGC 3 cut(s) 221, 949, 1003
PleI GAGTC 3 cut(s) 335, 770, 812
PpsI GAGTC 3 cut(s) 335, 770, 812
Psp6I CCWGG 2 cut(s) 13, 693
PspGI CCWGG 2 cut(s) 13, 693
PspN4I GGNNCC 4 cut(s) 450, 459, 522, 838
PspPI GGNCC 3 cut(s) 228, 601, 829
PstI CTGCAG 1 cut(s) 937
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 288
RruI TCGCGA 1 cut(s) 985
SaqAI TTAA 4 cut(s) 527, 618, 1091, 1113
SatI GCNGC 3 cut(s) 220, 948, 1002
Sau3AI GATC 5 cut(s) 282, 288, 894, 964, 1009
Sau96I GGNCC 3 cut(s) 228, 601, 829
SchI GAGTC 3 cut(s) 335, 770, 813
ScrFI CCNGG 2 cut(s) 15, 695
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 2 cut(s) 625, 673
SfcI CTRYAG 2 cut(s) 578, 933
SfuI TTCGAA 2 cut(s) 21, 169
SinI GGWCC 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 617, 1005
SmoI CTYRAG 2 cut(s) 617, 1005
Sse9I AATT 2 cut(s) 853, 954
SsiI CCGC 3 cut(s) 220, 298, 539
SspI AATATT 3 cut(s) 879, 1089, 1111
SspMI CTAG 3 cut(s) 87, 453, 1064
StyD4I CCNGG 2 cut(s) 13, 693
StyI CCWWGG 5 cut(s) 444, 452, 780, 832, 921
TaaI ACNGT 3 cut(s) 535, 582, 979
TaiI ACGT 1 cut(s) 241
TaqI TCGA 4 cut(s) 21, 136, 169, 328
TasI AATT 2 cut(s) 853, 954
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 4 cut(s) 349, 641, 690, 971
Tru1I TTAA 4 cut(s) 527, 618, 1091, 1113
Tru9I TTAA 4 cut(s) 527, 618, 1091, 1113
TscAI CASTG 2 cut(s) 133, 852
TseI GCWGC 2 cut(s) 947, 1001
TspDTI ATGAA 3 cut(s) 747, 847, 1125
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 2 cut(s) 133, 852
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 228
XagI CCTNNNNNAGG 1 cut(s) 608
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 1 cut(s) 575
XmaJI CCTAGG 1 cut(s) 452
XmnI GAANNNNTTC 2 cut(s) 612, 645
XspI CTAG 3 cut(s) 87, 453, 1064
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.