Rh2AG528900

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000951
Physical Location & Seq
Reverse (-)
75733986 .. 75736825
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG528900.1

Sequence Viewer

Length: 1230 bp
ATGGCCGGAGAAGGAGTATTCTGTCACCAGTGCCGCCAAAGGACCACGAAACGTGTGTTCTTGTGCAACAATGTCATGGGGAAACGCCAATGCAAGATCAAGATCTGCGAGCGGTGCCTCAAGAAAAGATATGTAGATATTACAGAAGAGGCGGCTGCTTCAGATGATTGGAAGTGTCCGAAATGCTGCGGGATCTGCAACTGCAGTGTGTGCATGAAGCGAGAGGGTAAAGAACCTCTTGGTCGAGCTGTGCCTGACTCAGAGAATCCTTCAGAGGAGGAGCCTGCGGGAGTGTCTGAGCCAAATAGAGAGGAAGAAAACCAAAAAGAAGATGTTGGCAAGGGCAAGAGGAAGGCCAAGGCTCCTAGGGAACCTCGCAGAGTGAAGGCAAAGGAGGAAGTAGTTGAGCCTGAACTTCCTCTGCCTTTGGGGGTTCCCTTAACAACTGTTGCGGGCATTGAACTACATCCTCAGGATGTAGGACATGCCCTACAGTTTATAGAGTTCTGTGTGGCCCTTGAAGAGGTTCTTAAGTTCAGCAAAGAAGATGCTGAATCTCTTCTCAGATACCTAGCTAATGCATCAACGATACTCCTCAGTAGTCCAATTCCAGGCCAAACTCCTGAACTGCCCTCAGATTTCTTTAGTAGAGGAGTTGAGGGGTATGACTCCTTGGACTTCTCCCAGAAGCTTAGAGTCCTAACTTTCCTTTGTGATGAGGCCCTTGGCACCAAATCACTGAGCAATTTCATAGAAAGCCAAAAAGAAATATTCTCTAAAAGTAAGATCGCAGCAAAACGAAAGTATCTGCTGCGAAGGCTAAGTTCAGCAGCTCAAGATCATGCAGAGTTGCTTGAAGCAAAAGCCTTGAAATCAAGGATGATACGACAAGATTCTGTGCGAACAGAGCCTTATCTGTTCGATGCTGATGGTTGTGCATTTTGGAGATTGACAGGCTATGGTGATGGAGATGATGTGCTGCTTCAAGATGTAGGAACTTGGGATCCATTTACATCTTCTGATGAAAAATGGTTCCAGTATGGGGCAGAAAAGAAAGAAGCAGTTGAAAAATACTGCTCCTGTAAGGACAAGAGGCCTTTATGTCTGAAACCCTGTCTCAAACACTTCCTTCAAGTTCTGGAACACTTCCTTCAAGTTCTGGAAGTAAGGAAGAAGAGAAGCTTGAAAATGCGACTACTGGAAGCAGAGAAGAAGACATACCCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

409

Amino Acids

46.41

Weight (kDa)

8.29

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 4 - 84 9.8e-21 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 114, 728
AccBSI CCGCTC 1 cut(s) 112
AciI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
AclWI GGATC 3 cut(s) 200, 998, 1011
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 144, 255
AfiI CCNNNNNNNGG 3 cut(s) 523, 611, 1042
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 9 cut(s) 461, 521, 857, 871, 986, 1067, 1133, 1154, 1186
AjnI CCWGG 1 cut(s) 610
AloI GAACNNNNNNTCC 2 cut(s) 988, 1020
AluBI AGCT 5 cut(s) 248, 575, 691, 833, 1182
AluI AGCT 5 cut(s) 248, 575, 691, 833, 1182
Alw26I GTCTC 1 cut(s) 1121
AlwI GGATC 3 cut(s) 200, 998, 1011
AoxI GGCC 6 cut(s) 3, 354, 513, 613, 720, 1094
ApeKI GCWGC 6 cut(s) 155, 186, 791, 811, 830, 979
Asp700I GAANNNNTTC 2 cut(s) 525, 558
AspA2I CCTAGG 1 cut(s) 365
AspS9I GGNCC 3 cut(s) 42, 514, 721
AsuHPI GGTGA 2 cut(s) 17, 974
AvaII GGWCC 1 cut(s) 42
AvrII CCTAGG 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 788, 821
BamHI GGATCC 1 cut(s) 1003
BanI GGYRCC 2 cut(s) 114, 728
BarI GAAGNNNNNNTAC 1 cut(s) 1202
BbsI GAAGAC 1 cut(s) 1220
BbvI GCAGC 6 cut(s) 142, 173, 798, 803, 842, 966
BccI CCATC 2 cut(s) 923, 959
BciT130I CCWGG 1 cut(s) 612
BcoDI GTCTC 1 cut(s) 1121
BfaI CTAG 2 cut(s) 366, 572
BfmI CTRYAG 2 cut(s) 202, 491
BfrI CTTAAG 1 cut(s) 530
BglII AGATCT 1 cut(s) 102
BisI GCNGC 8 cut(s) 34, 153, 156, 187, 792, 812, 831, 980
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 8 cut(s) 35, 154, 157, 188, 793, 813, 832, 981
Bme1390I CCNGG 1 cut(s) 612
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 3 cut(s) 42, 514, 721
BmiI GGNNCC 8 cut(s) 116, 282, 363, 372, 435, 730, 1005, 1034
BmrFI CCNGG 1 cut(s) 612
BmsI GCATC 3 cut(s) 538, 590, 913
BpiI GAAGAC 1 cut(s) 1220
BpuEI CTTGAG 2 cut(s) 104, 819
BsaBI GATNNNNATC 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 357, 365, 672, 724
BsaXI ACNNNNNCTCC 3 cut(s) 30, 960, 990
Bsc4I CCNNNNNNNGG 3 cut(s) 523, 611, 1042
Bse1I ACTGG 3 cut(s) 28, 1036, 1203
Bse21I CCTNAGG 1 cut(s) 471
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 4 cut(s) 357, 365, 672, 724
BseGI GGATG 3 cut(s) 466, 481, 885
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 3 cut(s) 523, 611, 1042
BseMII CTCAG 7 cut(s) 273, 288, 485, 577, 610, 648, 731
BseNI ACTGG 3 cut(s) 28, 1036, 1203
BseRI GAGGAG 4 cut(s) 290, 293, 584, 666
BseXI GCAGC 6 cut(s) 142, 173, 798, 803, 842, 966
BshFI GGCC 6 cut(s) 5, 356, 515, 615, 722, 1096
BshNI GGYRCC 2 cut(s) 114, 728
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 3 cut(s) 523, 611, 1042
BsmAI GTCTC 1 cut(s) 1121
BsnI GGCC 6 cut(s) 5, 356, 515, 615, 722, 1096
Bsp143I GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
BspACI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
BspANI GGCC 6 cut(s) 5, 356, 515, 615, 722, 1096
BspCNI CTCAG 7 cut(s) 272, 289, 484, 576, 609, 647, 732
BspLI GGNNCC 8 cut(s) 116, 282, 363, 372, 435, 730, 1005, 1034
BspMAI CTGCAG 1 cut(s) 206
BspPI GGATC 3 cut(s) 200, 998, 1011
BspT107I GGYRCC 2 cut(s) 114, 728
BspTI CTTAAG 1 cut(s) 530
BsrBI CCGCTC 1 cut(s) 112
BsrI ACTGG 3 cut(s) 28, 1036, 1203
BssECI CCNNGG 4 cut(s) 357, 365, 672, 724
BssMI GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
BssT1I CCWWGG 4 cut(s) 357, 365, 672, 724
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 2 cut(s) 448, 495
Bst6I CTCTTC 4 cut(s) 141, 516, 564, 1169
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 110, 285, 454
BstDEI CTNAG 9 cut(s) 259, 297, 471, 563, 596, 634, 692, 740, 821
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 3 cut(s) 466, 481, 885
BstKTI GATC 6 cut(s) 99, 105, 195, 789, 841, 1006
BstMAI GTCTC 1 cut(s) 1121
BstMBI GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
BstMWI GCNNNNNNNGC 5 cut(s) 114, 195, 210, 817, 907
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 610
BstSFI CTRYAG 2 cut(s) 202, 491
BstV1I GCAGC 6 cut(s) 142, 173, 798, 803, 842, 966
BstV2I GAAGAC 1 cut(s) 1220
BstX2I RGATCY 3 cut(s) 102, 192, 1003
BstYI RGATCY 3 cut(s) 102, 192, 1003
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 6 cut(s) 5, 356, 515, 615, 722, 1096
BtsCI GGATG 3 cut(s) 466, 481, 885
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 3 cut(s) 35, 211, 737
Cac8I GCNNGC 3 cut(s) 110, 285, 454
Cfr13I GGNCC 3 cut(s) 42, 514, 721
CviAII CATG 4 cut(s) 76, 214, 485, 842
DdeI CTNAG 9 cut(s) 259, 297, 471, 563, 596, 634, 692, 740, 821
DpnI GATC 6 cut(s) 98, 104, 194, 788, 840, 1005
DpnII GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 141, 516, 564, 1169
EarI CTCTTC 4 cut(s) 141, 516, 564, 1169
Eco130I CCWWGG 4 cut(s) 357, 365, 672, 724
Eco147I AGGCCT 1 cut(s) 1096
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 144, 255
Eco81I CCTNAGG 1 cut(s) 471
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 4 cut(s) 357, 365, 672, 724
EcoT22I ATGCAT 1 cut(s) 583
ErhI CCWWGG 4 cut(s) 357, 365, 672, 724
FaeI CATG 4 cut(s) 79, 217, 488, 845
FalI AAGNNNNNCTT 6 cut(s) 222, 254, 513, 545, 1166, 1198
FatI CATG 4 cut(s) 75, 213, 484, 841
FauI CCCGC 3 cut(s) 182, 280, 445
Fnu4HI GCNGC 8 cut(s) 34, 153, 156, 187, 792, 812, 831, 980
FokI GGATG 3 cut(s) 453, 488, 892
Fsp4HI GCNGC 8 cut(s) 34, 153, 156, 187, 792, 812, 831, 980
FspBI CTAG 2 cut(s) 366, 572
GluI GCNGC 8 cut(s) 34, 153, 156, 187, 792, 812, 831, 980
HaeIII GGCC 6 cut(s) 5, 356, 515, 615, 722, 1096
HapII CCGG 1 cut(s) 6
Hin1II CATG 4 cut(s) 79, 217, 488, 845
HindIII AAGCTT 2 cut(s) 689, 1180
HinfI GANTC 6 cut(s) 257, 265, 554, 668, 696, 893
HpaII CCGG 1 cut(s) 6
HphI GGTGA 2 cut(s) 17, 974
Hpy188I TCNGA 9 cut(s) 163, 180, 262, 274, 298, 566, 637, 1021, 1107
Hpy188III TCNNGA 8 cut(s) 100, 121, 473, 623, 836, 986, 1139, 1160
HpyAV CCTTC 7 cut(s) 5, 279, 346, 379, 810, 1139, 1160
HpyCH4III ACNGT 2 cut(s) 448, 495
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 8 cut(s) 66, 93, 198, 204, 213, 581, 845, 938
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 195, 210, 817, 907
HpyF3I CTNAG 9 cut(s) 259, 297, 471, 563, 596, 634, 692, 740, 821
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 4 cut(s) 79, 217, 488, 845
Kzo9I GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
LmnI GCTCC 3 cut(s) 280, 367, 1082
Lsp1109I GCAGC 6 cut(s) 142, 173, 798, 803, 842, 966
LweI GCATC 3 cut(s) 538, 590, 913
MaeI CTAG 2 cut(s) 366, 572
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 1 cut(s) 23
MalI GATC 6 cut(s) 98, 104, 194, 788, 840, 1005
MbiI CCGCTC 1 cut(s) 112
MboI GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
MflI RGATCY 3 cut(s) 102, 192, 1003
MluCI AATT 2 cut(s) 606, 745
MlyI GAGTC 3 cut(s) 251, 662, 705
Mph1103I ATGCAT 1 cut(s) 583
MroXI GAANNNNTTC 2 cut(s) 525, 558
MseI TTAA 2 cut(s) 440, 531
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 612
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 5 cut(s) 114, 195, 210, 817, 907
NdeII GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
NlaIII CATG 4 cut(s) 79, 217, 488, 845
NlaIV GGNNCC 8 cut(s) 116, 282, 363, 372, 435, 730, 1005, 1034
NmuCI GTSAC 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 1 cut(s) 488
PceI AGGCCT 1 cut(s) 1096
PdmI GAANNNNTTC 2 cut(s) 525, 558
PfeI GAWTC 3 cut(s) 265, 554, 893
PkrI GCNGC 8 cut(s) 35, 154, 157, 188, 793, 813, 832, 981
PleI GAGTC 3 cut(s) 251, 662, 704
PpsI GAGTC 3 cut(s) 251, 662, 704
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 8 cut(s) 116, 282, 363, 372, 435, 730, 1005, 1034
PspPI GGNCC 3 cut(s) 42, 514, 721
PstI CTGCAG 1 cut(s) 206
PsuI RGATCY 3 cut(s) 102, 192, 1003
SaqAI TTAA 2 cut(s) 440, 531
SatI GCNGC 8 cut(s) 34, 153, 156, 187, 792, 812, 831, 980
Sau3AI GATC 6 cut(s) 96, 102, 192, 786, 838, 1003
Sau96I GGNCC 3 cut(s) 42, 514, 721
SchI GAGTC 3 cut(s) 251, 662, 705
ScrFI CCNGG 1 cut(s) 612
SfaNI GCATC 3 cut(s) 538, 590, 913
SfcI CTRYAG 2 cut(s) 202, 491
SinI GGWCC 1 cut(s) 42
SmlI CTYRAG 3 cut(s) 119, 530, 834
SmoI CTYRAG 3 cut(s) 119, 530, 834
Sse9I AATT 2 cut(s) 606, 745
SseBI AGGCCT 1 cut(s) 1096
SsiI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
SspI AATATT 1 cut(s) 771
SspMI CTAG 2 cut(s) 366, 572
StuI AGGCCT 1 cut(s) 1096
StyD4I CCNGG 1 cut(s) 610
StyI CCWWGG 4 cut(s) 357, 365, 672, 724
TaaI ACNGT 2 cut(s) 448, 495
TaiI ACGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 244, 921
TasI AATT 2 cut(s) 606, 745
TauI GCSGC 2 cut(s) 36, 155
TfiI GAWTC 3 cut(s) 265, 554, 893
Tru1I TTAA 2 cut(s) 440, 531
Tru9I TTAA 2 cut(s) 440, 531
TscAI CASTG 3 cut(s) 35, 211, 744
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 6 cut(s) 155, 186, 791, 811, 830, 979
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 3 cut(s) 230, 739, 1038
TspRI CASTG 3 cut(s) 35, 211, 744
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 521
XceI RCATGY 1 cut(s) 488
XmaJI CCTAGG 1 cut(s) 365
XmnI GAANNNNTTC 2 cut(s) 525, 558
XspI CTAG 2 cut(s) 366, 572
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.