RLG00000002324

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000951
Physical Location & Seq
Forward (+)
32189168 .. 32192311
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002324

Sequence Viewer

Length: 1080 bp
ATGGCCGGAGAAGGAGTATTCAGTCACCAGTGCTGCCAAAGGACCATGAGACATGTGTTCTCATGCACTAGCATTATGGGGAGACGTCATGGCGCCAACAAGATCTGCCAGTCATGCCTCAAGAACAGGTACACGATAACAGATGAGGTGGCGGCTTCAGATGATTGGAAGTGCCCCAGATGCTGTGGGAATTGCAACTGCAGTACGTGTGTGAAGCGACAGGGCAAAGAACCTCGCAGTCGTAGTATGCTTGAACCCGAGAAGCCTTCAGACCAGAAGACGTTAAGGGCAAGCGGAAGTCCAAGGCTCCCAGAGCCCCGTAGAGTGAAAGCAAAGGAGGAAGTACTTAGGGTTGAACTGTCTCTACCTTTGGGGGTGCCCTTAACAACCGTTGCGGGCATTGCACTAGATCCTCAGGATGTTGGACATGCCCTACAATTTATAGAGTTCTGTGACGCCTTCCAAGAGGTTCTTGAGTTCAGCACAGCAGAAGTTGAATCTCTTCTTCGAGACCTGATGCGTCAAGAATACTCCTCAGTAGTTCTATTTCAAGCCAAACTCCTGTCTTTGATACATAAAAAGAAGATCGGAGAAGAGCATTCGGACGAAAATTCATGGTTAGAAGTTTTGAAAAACTGCTTCTCCAAGTCTCAATTCCGACTAAGGGTATTGCCCACAGATGTGTTTAGTAGAGAAGTTGACGGATACGACTCTTTGCACTTCCCCTTGAAGCTTAGAGTGCCGGTTCGTCAGCGACGAGCTGAGCTGTGGCCGGTTGATGACGGGTCGTCTCCAATCGGGTCGGAGTCTTATCGGACTCCGTCTCCGTTTAATCGGAGCAATTCGTCGATTTGCTTCCGACCGCGCAGAGCTGCTGCCCCTGCCCTAAAATCCCAACCCGGATTTCTCGATGACGTGGCCGTCAATCTCAGTAGGGTTTTCGTACTCTCTGTCCTAGATTCCGAGTCTGTTCTCGAGGCCCTCAGAACAGAACGCGAAAACTTCGCAGAAGAATTGCAACGCCAGAAGATCCGTACTCTAATGTCCATGCGGTTTCTAACCCAACAACGATCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

360

Amino Acids

40.77

Weight (kDa)

8.83

Isoelectric Point (pI)

59.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 5 - 80 5.3e-14 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 920
AatII GACGTC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 92, 376
AccII CGCG 2 cut(s) 865, 996
AciI CCGC 5 cut(s) 152, 294, 395, 863, 1051
AclWI GGATC 3 cut(s) 404, 1024, 1065
AcoI YGGCCR 3 cut(s) 3, 770, 918
AcsI RAATTY 1 cut(s) 610
AcuI CTGAAG 2 cut(s) 141, 252
AcyI GRCGYC 3 cut(s) 85, 93, 456
AfaI GTAC 5 cut(s) 131, 205, 345, 945, 1036
AfiI CCNNNNNNNGG 1 cut(s) 664
AflIII ACRYGT 2 cut(s) 52, 206
AgsI TTSAA 6 cut(s) 254, 356, 497, 551, 631, 730
AhdI GACNNNNNGTC 1 cut(s) 787
AjiI CACGTC 1 cut(s) 916
AjuI GAANNNNNNNTTGG 4 cut(s) 638, 670, 888, 920
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 4 cut(s) 733, 761, 766, 872
AluI AGCT 4 cut(s) 733, 761, 766, 872
Alw26I GTCTC 7 cut(s) 43, 76, 366, 504, 654, 795, 828
AlwI GGATC 3 cut(s) 404, 1024, 1065
AlwNI CAGNNNCTG 1 cut(s) 183
Ama87I CYCGRG 2 cut(s) 257, 974
AoxI GGCC 4 cut(s) 3, 770, 918, 978
ApeKI GCWGC 3 cut(s) 33, 872, 875
ApoI RAATTY 1 cut(s) 610
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 2 cut(s) 95, 867
AspS9I GGNCC 2 cut(s) 42, 979
AsuC2I CCSGG 1 cut(s) 900
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 2 cut(s) 257, 974
AvaII GGWCC 1 cut(s) 42
AxyI CCTNAGG 1 cut(s) 414
BaeGI GKGCMC 2 cut(s) 176, 381
BanI GGYRCC 2 cut(s) 92, 376
BanII GRGCYC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 20, 859, 862
BceAI ACGGC 1 cut(s) 905
BciVI GTATCC 1 cut(s) 698
BcnI CCSGG 1 cut(s) 900
BcoDI GTCTC 7 cut(s) 43, 76, 366, 504, 654, 795, 828
BfaI CTAG 3 cut(s) 69, 407, 956
BfmI CTRYAG 1 cut(s) 199
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 1 cut(s) 698
BglII AGATCT 1 cut(s) 102
BisI GCNGC 4 cut(s) 34, 153, 873, 876
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 4 cut(s) 35, 154, 874, 877
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 1 cut(s) 900
Bme18I GGWCC 1 cut(s) 42
BmeRI GACNNNNNGTC 1 cut(s) 787
BmeT110I CYCGRG 2 cut(s) 257, 974
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 42, 979
BmiI GGNNCC 3 cut(s) 94, 308, 378
BmrFI CCNGG 1 cut(s) 900
BmsI GCATC 2 cut(s) 170, 507
BpiI GAAGAC 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 2 cut(s) 104, 494
BpuMI CCSGG 1 cut(s) 900
BsaAI YACGTR 1 cut(s) 207
BsaHI GRCGYC 3 cut(s) 85, 93, 456
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 302
BsaXI ACNNNNNCTCC 5 cut(s) 30, 626, 656, 808, 838
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse118I RCCGGY 2 cut(s) 742, 772
Bse1I ACTGG 2 cut(s) 28, 109
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 5 cut(s) 428, 549, 753, 943, 997
BseNI ACTGG 2 cut(s) 28, 109
BseRI GAGGAG 1 cut(s) 523
BseSI GKGCMC 2 cut(s) 176, 381
BseXI GCAGC 3 cut(s) 20, 859, 862
Bsh1236I CGCG 2 cut(s) 865, 996
Bsh1285I CGRYCG 1 cut(s) 863
BshFI GGCC 4 cut(s) 5, 772, 920, 980
BshNI GGYRCC 2 cut(s) 92, 376
BsiEI CGRYCG 1 cut(s) 863
BsiHKCI CYCGRG 2 cut(s) 257, 974
BsiSI CCGG 4 cut(s) 6, 743, 773, 900
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 7 cut(s) 43, 76, 366, 504, 654, 795, 828
BsmBI CGTCTC 3 cut(s) 76, 795, 828
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 4 cut(s) 5, 772, 920, 980
Bso31I GGTCTC 1 cut(s) 504
BsoBI CYCGRG 2 cut(s) 257, 974
Bsp1286I GDGCHC 3 cut(s) 176, 318, 381
Bsp143I GATC 5 cut(s) 102, 409, 585, 1029, 1070
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 5 cut(s) 152, 294, 395, 863, 1051
BspANI GGCC 4 cut(s) 5, 772, 920, 980
BspCNI CTCAG 5 cut(s) 427, 548, 754, 942, 996
BspFNI CGCG 2 cut(s) 865, 996
BspLI GGNNCC 3 cut(s) 94, 308, 378
BspMAI CTGCAG 1 cut(s) 203
BspPI GGATC 3 cut(s) 404, 1024, 1065
BspQI GCTCTTC 1 cut(s) 588
BspT107I GGYRCC 2 cut(s) 92, 376
BspTNI GGTCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 2 cut(s) 742, 772
BsrI ACTGG 2 cut(s) 28, 109
BssAI RCCGGY 2 cut(s) 742, 772
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 5 cut(s) 102, 409, 585, 1029, 1070
BssNI GRCGYC 3 cut(s) 85, 93, 456
BssT1I CCWWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 360, 391
Bst6I CTCTTC 2 cut(s) 507, 588
BstACI GRCGYC 3 cut(s) 85, 93, 456
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 292, 397
BstDEI CTNAG 8 cut(s) 347, 414, 535, 662, 734, 762, 929, 983
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 2 cut(s) 865, 996
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 867
BstKTI GATC 5 cut(s) 105, 412, 588, 1032, 1073
BstMAI GTCTC 7 cut(s) 43, 76, 366, 504, 654, 795, 828
BstMBI GATC 5 cut(s) 102, 409, 585, 1029, 1070
BstMCI CGRYCG 1 cut(s) 863
BstMWI GCNNNNNNNGC 6 cut(s) 114, 180, 313, 401, 739, 881
BstNSI RCATGY 2 cut(s) 56, 431
BstSCI CCNGG 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 199
BstSLI GKGCMC 2 cut(s) 176, 381
BstUI CGCG 2 cut(s) 865, 996
BstV1I GCAGC 3 cut(s) 20, 859, 862
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 3 cut(s) 102, 409, 1029
BstYI RGATCY 3 cut(s) 102, 409, 1029
Bsu36I CCTNAGG 1 cut(s) 414
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 4 cut(s) 5, 772, 920, 980
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 292, 397
CaiI CAGNNNCTG 1 cut(s) 183
CfoI GCGC 2 cut(s) 95, 867
Cfr10I RCCGGY 2 cut(s) 742, 772
Cfr13I GGNCC 2 cut(s) 42, 979
CseI GACGC 2 cut(s) 464, 509
Csp6I GTAC 5 cut(s) 130, 204, 344, 944, 1035
CviAII CATG 8 cut(s) 46, 53, 63, 89, 114, 428, 615, 1048
CviQI GTAC 5 cut(s) 130, 204, 344, 944, 1035
DdeI CTNAG 8 cut(s) 347, 414, 535, 662, 734, 762, 929, 983
DinI GGCGCC 1 cut(s) 94
DpnI GATC 5 cut(s) 104, 411, 587, 1031, 1072
DpnII GATC 5 cut(s) 102, 409, 585, 1029, 1070
DrdI GACNNNNNNGTC 1 cut(s) 920
DriI GACNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 920
EaeI YGGCCR 3 cut(s) 3, 770, 918
Eam1104I CTCTTC 2 cut(s) 507, 588
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 2 cut(s) 507, 588
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 141, 252
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 2 cut(s) 257, 974
EcoO109I RGGNCCY 1 cut(s) 979
EcoT14I CCWWGG 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 318
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 302
Esp3I CGTCTC 3 cut(s) 76, 795, 828
FaeI CATG 8 cut(s) 49, 56, 66, 92, 117, 431, 618, 1051
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FatI CATG 8 cut(s) 45, 52, 62, 88, 113, 427, 614, 1047
FauI CCCGC 1 cut(s) 388
Fnu4HI GCNGC 4 cut(s) 34, 153, 873, 876
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 4 cut(s) 34, 153, 873, 876
FspBI CTAG 3 cut(s) 69, 407, 956
GlaI GCGC 2 cut(s) 94, 866
GluI GCNGC 4 cut(s) 34, 153, 873, 876
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 5, 772, 920, 980
HapII CCGG 4 cut(s) 6, 743, 773, 900
HgaI GACGC 2 cut(s) 464, 509
HhaI GCGC 2 cut(s) 95, 867
Hin1I GRCGYC 3 cut(s) 85, 93, 456
Hin1II CATG 8 cut(s) 49, 56, 66, 92, 117, 431, 618, 1051
Hin6I GCGC 2 cut(s) 93, 865
HinP1I GCGC 2 cut(s) 93, 865
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 6 cut(s) 497, 710, 806, 817, 959, 965
HpaII CCGG 4 cut(s) 6, 743, 773, 900
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 132, 700
Hpy188III TCNNGA 7 cut(s) 121, 416, 473, 509, 524, 908, 974
Hpy8I GTNNAC 2 cut(s) 132, 700
Hpy99I CGWCG 2 cut(s) 759, 850
HpyAV CCTTC 3 cut(s) 5, 276, 469
HpyCH4III ACNGT 2 cut(s) 360, 391
HpyCH4IV ACGT 4 cut(s) 85, 206, 281, 915
HpyCH4V TGCA 6 cut(s) 66, 195, 201, 404, 718, 1018
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 180, 313, 401, 739, 881
HpyF3I CTNAG 8 cut(s) 347, 414, 535, 662, 734, 762, 929, 983
HpySE526I ACGT 4 cut(s) 85, 206, 281, 915
Hsp92I GRCGYC 3 cut(s) 85, 93, 456
Hsp92II CATG 8 cut(s) 49, 56, 66, 92, 117, 431, 618, 1051
HspAI GCGC 2 cut(s) 93, 865
KasI GGCGCC 1 cut(s) 92
Kzo9I GATC 5 cut(s) 102, 409, 585, 1029, 1070
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 2 cut(s) 312, 837
Lsp1109I GCAGC 3 cut(s) 20, 859, 862
LweI GCATC 2 cut(s) 170, 507
MaeI CTAG 3 cut(s) 69, 407, 956
MaeII ACGT 4 cut(s) 85, 206, 281, 915
MaeIII GTNAC 2 cut(s) 23, 452
MalI GATC 5 cut(s) 104, 411, 587, 1031, 1072
MboI GATC 5 cut(s) 102, 409, 585, 1029, 1070
MboII GAAGA 7 cut(s) 289, 494, 497, 595, 605, 1022, 1039
MfeI CAATTG 1 cut(s) 1075
MflI RGATCY 3 cut(s) 102, 409, 1029
MhlI GDGCHC 3 cut(s) 176, 318, 381
MluCI AATT 7 cut(s) 190, 437, 610, 653, 841, 1013, 1075
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 4 cut(s) 704, 811, 815, 974
MmeI TCCRAC 4 cut(s) 403, 682, 783, 883
MnlI CCTC 9 cut(s) 128, 139, 243, 331, 423, 460, 544, 970, 992
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 3 cut(s) 284, 383, 831
MslI CAYNNNNRTG 1 cut(s) 680
MspI CCGG 4 cut(s) 6, 743, 773, 900
MspR9I CCNGG 1 cut(s) 900
MunI CAATTG 1 cut(s) 1075
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 2 cut(s) 865, 996
MwoI GCNNNNNNNGC 6 cut(s) 114, 180, 313, 401, 739, 881
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 900
NdeII GATC 5 cut(s) 102, 409, 585, 1029, 1070
NlaIII CATG 8 cut(s) 49, 56, 66, 92, 117, 431, 618, 1051
NlaIV GGNNCC 3 cut(s) 94, 308, 378
NmuCI GTSAC 2 cut(s) 23, 452
NspI RCATGY 2 cut(s) 56, 431
OliI CACNNNNGTG 1 cut(s) 680
PaeR7I CTCGAG 1 cut(s) 974
PciI ACATGT 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 754
PctI GAATGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 2 cut(s) 497, 959
PflFI GACNNNGTC 1 cut(s) 820
PkrI GCNGC 4 cut(s) 35, 154, 874, 877
PleI GAGTC 4 cut(s) 704, 811, 814, 973
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 4 cut(s) 704, 811, 814, 973
Ppu21I YACGTR 1 cut(s) 207
PscI ACATGT 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 94, 308, 378
PspPI GGNCC 2 cut(s) 42, 979
PsrI GAACNNNNNNTAC 2 cut(s) 348, 380
PstI CTGCAG 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 183
PsuI RGATCY 3 cut(s) 102, 409, 1029
PsyI GACNNNGTC 1 cut(s) 820
RsaI GTAC 5 cut(s) 131, 205, 345, 945, 1036
RsaNI GTAC 5 cut(s) 130, 204, 344, 944, 1035
RseI CAYNNNNRTG 1 cut(s) 680
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 3 cut(s) 284, 383, 831
SatI GCNGC 4 cut(s) 34, 153, 873, 876
Sau3AI GATC 5 cut(s) 102, 409, 585, 1029, 1070
Sau96I GGNCC 2 cut(s) 42, 979
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 4 cut(s) 704, 811, 815, 974
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 3 cut(s) 176, 318, 381
SfaNI GCATC 2 cut(s) 170, 507
SfcI CTRYAG 1 cut(s) 199
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 974
SinI GGWCC 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 3 cut(s) 119, 473, 974
SmoI CTYRAG 3 cut(s) 119, 473, 974
Sse9I AATT 7 cut(s) 190, 437, 610, 653, 841, 1013, 1075
SsiI CCGC 5 cut(s) 152, 294, 395, 863, 1051
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 3 cut(s) 69, 407, 956
StyD4I CCNGG 1 cut(s) 898
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 360, 391
TaiI ACGT 4 cut(s) 88, 209, 284, 918
TaqI TCGA 4 cut(s) 508, 848, 909, 975
TasI AATT 7 cut(s) 190, 437, 610, 653, 841, 1013, 1075
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 2 cut(s) 497, 959
Tru1I TTAA 3 cut(s) 284, 383, 831
Tru9I TTAA 3 cut(s) 284, 383, 831
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 2 cut(s) 23, 452
TseI GCWGC 3 cut(s) 33, 872, 875
Tsp45I GTSAC 2 cut(s) 23, 452
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 4 cut(s) 717, 810, 816, 1022
TspRI CASTG 1 cut(s) 35
Tth111I GACNNNGTC 1 cut(s) 820
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 610
XceI RCATGY 2 cut(s) 56, 431
XhoI CTCGAG 1 cut(s) 974
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 3 cut(s) 69, 407, 956
ZraI GACGTC 1 cut(s) 86
ZrmI AGTACT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.