Rroxscaffold_4G00310990

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000951
Physical Location & Seq
Forward (+)
33164134 .. 33166875
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310990.1

Sequence Viewer

Length: 1182 bp
ATGAAGTCTCCGAATCCGAGAGAAGGAGAAGCTTCTGAAACAGAGTTTAGATGTCGCCAAAGGACCGCGAAACGTGTGTTCTTCTGCAACAACGTCATGGGGAAACGCCAATGCAAGATCAAGACCTGCGAGCGATGCCTCAAGAAAAGATATGTAGATATCATAGAAGAGGCGGCTGCGTCAGATGATTGGAAGTGTCCGAAATGCTGCGGTATCTGCAACTGCAGTGTGTGCATGAAGCGAGAGGGTAAAGAACCTCTAGATCGAGTTGTGCCTGACTCTGAGAAGCCTTCAGAGGAGGAGCCTGCGGAAGTGGCTGAGCCAAATGGAGGAGAAGAAAACCAAAGAGAAGATGTTGGTAAGGGCAAGCGGAAGGCCAAGGCTCTGTGGGAACCCTGCAGAGTGAAGGCGAAGGAAGAAGTAGTTAAGCCGGAACTTCCTCTACCTTTGGGGGTTCCCTTAACAACTGTTGCGGGCATTGAACTACATCCTCAGGATGTTCTTAAGTTCGGCAAAGAAGATGCTGAATCTCTGCTGAATCTCTTCTCAGAGACCAAATGCATCAACAATACTCCTCAGTATTCCGATTCCAGGCCAAACTCCTGTCTGAGATACAGAAGACAGGAGACAGGAGTTGAGGGGTATTACTCTTTGGACTTCTCCCAGAAGCTTAGAGTCCTAACTTTCCTTTGTGATGAGGCTCTTGACACCAAATCACTAGCAAATTCATCGAAAGCCAAAAAGACATATTCCATCGAAGCAAGATCGCAGCAAAACGAACAGTATCTGCTGCGAAGGCTAAGTATCAAGCAGCAGTTGAAAGCAGAGGTGGCCAAGGCCAAGGCCAAGGCATCTACTGCAGGAGACAATGCTGCTTCAACTTCTTCAGATCACGAATCTACTGTTTTGCGACTGAAGAGTGAAGCAGCTCAAGCTCATGCAGAGTTGCCTGAAGCAAAATCCTTGAAATCAAGGATGATACGACATGATTCTATGCGAACAGTGCCTTATTTGTTCGATGCTGATAGTCGTGCATTTTGGAGATTGACAAGCTATGGTGATGGAGATGATGTGTTGCTTCAAGATGTGGGAACTTGGGATCCATTTACATCTTCTGATGAAAAAGAGAGAAGGAGTCCAAAAATACTGCTCTTCTGCAAGCACAAGAGACCTTATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

393

Amino Acids

44.47

Weight (kDa)

8.65

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 12 - 92 7.6e-18 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 134
AccII CGCG 1 cut(s) 68
AciI CCGC 6 cut(s) 66, 173, 210, 308, 370, 473
AclWI GGATC 2 cut(s) 1094, 1107
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 1 cut(s) 724
AcuI CTGAAG 4 cut(s) 276, 870, 935, 972
AfiI CCNNNNNNNGG 3 cut(s) 23, 329, 591
AflII CTTAAG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 5 cut(s) 482, 820, 879, 967, 1082
AhdI GACNNNNNGTC 1 cut(s) 1175
AjnI CCWGG 1 cut(s) 590
AloI GAACNNNNNNTCC 2 cut(s) 1084, 1116
AluBI AGCT 5 cut(s) 32, 670, 929, 935, 1053
AluI AGCT 5 cut(s) 32, 670, 929, 935, 1053
Alw26I GTCTC 5 cut(s) 12, 545, 620, 858, 1162
AlwI GGATC 2 cut(s) 1094, 1107
AlwNI CAGNNNCTG 1 cut(s) 787
AoxI GGCC 5 cut(s) 375, 593, 831, 837, 843
ApeKI GCWGC 7 cut(s) 176, 207, 769, 790, 811, 872, 926
ApoI RAATTY 1 cut(s) 724
Asp700I GAANNNNTTC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 1 cut(s) 1070
AvaII GGWCC 1 cut(s) 63
AxyI CCTNAGG 1 cut(s) 492
BalI TGGCCA 1 cut(s) 833
BamHI GGATCC 1 cut(s) 1099
BbsI GAAGAC 1 cut(s) 625
BbvI GCAGC 7 cut(s) 163, 194, 777, 781, 823, 859, 938
BccI CCATC 2 cut(s) 761, 1055
BcgI CGANNNNNNTGC 2 cut(s) 711, 745
BciT130I CCWGG 1 cut(s) 592
BcoDI GTCTC 5 cut(s) 12, 545, 620, 858, 1162
BfaI CTAG 2 cut(s) 260, 719
BfmI CTRYAG 3 cut(s) 223, 397, 858
BfrI CTTAAG 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 8 cut(s) 174, 177, 208, 770, 791, 812, 873, 927
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 8 cut(s) 175, 178, 209, 771, 792, 813, 874, 928
Bme1390I CCNGG 1 cut(s) 592
Bme18I GGWCC 1 cut(s) 63
BmeRI GACNNNNNGTC 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 4 cut(s) 303, 393, 456, 1101
BmrFI CCNGG 1 cut(s) 592
BmsI GCATC 5 cut(s) 125, 511, 570, 860, 1009
BpiI GAAGAC 1 cut(s) 625
Bpu1102I GCTNAGC 1 cut(s) 318
BpuEI CTTGAG 2 cut(s) 125, 915
BsaI GGTCTC 2 cut(s) 545, 1162
BsaJI CCNNGG 4 cut(s) 378, 834, 840, 846
BsaXI ACNNNNNCTCC 2 cut(s) 1056, 1086
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 329, 591
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 1 cut(s) 592
BseDI CCNNGG 4 cut(s) 378, 834, 840, 846
BseGI GGATG 3 cut(s) 487, 502, 981
BseLI CCNNNNNNNGG 3 cut(s) 23, 329, 591
BseMII CTCAG 6 cut(s) 273, 309, 506, 561, 590, 599
BseRI GAGGAG 4 cut(s) 311, 314, 345, 564
BseXI GCAGC 7 cut(s) 163, 194, 777, 781, 823, 859, 938
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 5 cut(s) 377, 595, 833, 839, 845
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 3 cut(s) 23, 329, 591
BsmAI GTCTC 5 cut(s) 12, 545, 620, 858, 1162
BsnI GGCC 5 cut(s) 377, 595, 833, 839, 845
Bso31I GGTCTC 2 cut(s) 545, 1162
Bsp143I GATC 5 cut(s) 117, 262, 764, 889, 1099
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 6 cut(s) 66, 173, 210, 308, 370, 473
BspANI GGCC 5 cut(s) 377, 595, 833, 839, 845
BspCNI CTCAG 6 cut(s) 274, 310, 505, 560, 589, 600
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 4 cut(s) 303, 393, 456, 1101
BspMAI CTGCAG 3 cut(s) 227, 401, 862
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 1094, 1107
BspQI GCTCTTC 1 cut(s) 1157
BspTI CTTAAG 1 cut(s) 503
BspTNI GGTCTC 2 cut(s) 545, 1162
BssECI CCNNGG 4 cut(s) 378, 834, 840, 846
BssMI GATC 5 cut(s) 117, 262, 764, 889, 1099
BssT1I CCWWGG 4 cut(s) 378, 834, 840, 846
Bst2UI CCWGG 1 cut(s) 592
Bst4CI ACNGT 4 cut(s) 469, 783, 904, 1003
Bst6I CTCTTC 4 cut(s) 162, 548, 911, 1157
BstAFI CTTAAG 1 cut(s) 503
BstAPI GCANNNNNTGC 2 cut(s) 231, 857
BstC8I GCNNGC 5 cut(s) 131, 306, 368, 475, 1160
BstDEI CTNAG 8 cut(s) 282, 318, 492, 547, 576, 608, 671, 800
BstF5I GGATG 3 cut(s) 487, 502, 981
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 5 cut(s) 120, 265, 767, 892, 1102
BstMAI GTCTC 5 cut(s) 12, 545, 620, 858, 1162
BstMBI GATC 5 cut(s) 117, 262, 764, 889, 1099
BstMWI GCNNNNNNNGC 9 cut(s) 135, 216, 231, 314, 796, 830, 857, 932, 1003
BstNI CCWGG 1 cut(s) 592
BstSCI CCNGG 1 cut(s) 590
BstSFI CTRYAG 3 cut(s) 223, 397, 858
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 7 cut(s) 163, 194, 777, 781, 823, 859, 938
BstV2I GAAGAC 1 cut(s) 625
BstX2I RGATCY 1 cut(s) 1099
BstYI RGATCY 1 cut(s) 1099
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 5 cut(s) 377, 595, 833, 839, 845
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 3 cut(s) 487, 502, 981
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 1008
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 5 cut(s) 131, 306, 368, 475, 1160
CaiI CAGNNNCTG 1 cut(s) 787
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 1 cut(s) 168
CviAII CATG 4 cut(s) 97, 235, 938, 986
DdeI CTNAG 8 cut(s) 282, 318, 492, 547, 576, 608, 671, 800
DpnI GATC 5 cut(s) 119, 264, 766, 891, 1101
DpnII GATC 5 cut(s) 117, 262, 764, 889, 1099
DriI GACNNNNNGTC 1 cut(s) 1175
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 4 cut(s) 162, 548, 911, 1157
Eam1105I GACNNNNNGTC 1 cut(s) 1175
EarI CTCTTC 4 cut(s) 162, 548, 911, 1157
Eco130I CCWWGG 4 cut(s) 378, 834, 840, 846
Eco31I GGTCTC 2 cut(s) 545, 1162
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 63
Eco57I CTGAAG 4 cut(s) 276, 870, 935, 972
Eco81I CCTNAGG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 590
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 4 cut(s) 378, 834, 840, 846
EcoT22I ATGCAT 1 cut(s) 563
ErhI CCWWGG 4 cut(s) 378, 834, 840, 846
FaeI CATG 4 cut(s) 100, 238, 941, 989
FatI CATG 4 cut(s) 96, 234, 937, 985
FauI CCCGC 1 cut(s) 466
Fnu4HI GCNGC 8 cut(s) 174, 177, 208, 770, 791, 812, 873, 927
FokI GGATG 3 cut(s) 474, 509, 988
Fsp4HI GCNGC 8 cut(s) 174, 177, 208, 770, 791, 812, 873, 927
FspBI CTAG 2 cut(s) 260, 719
GluI GCNGC 8 cut(s) 174, 177, 208, 770, 791, 812, 873, 927
HaeIII GGCC 5 cut(s) 377, 595, 833, 839, 845
HapII CCGG 1 cut(s) 431
HgaI GACGC 1 cut(s) 168
Hin1II CATG 4 cut(s) 100, 238, 941, 989
HindIII AAGCTT 2 cut(s) 30, 668
HinfI GANTC 9 cut(s) 13, 278, 527, 538, 587, 675, 896, 989, 1135
HpaII CCGG 1 cut(s) 431
HphI GGTGA 1 cut(s) 1070
Hpy188III TCNNGA 7 cut(s) 121, 142, 260, 494, 704, 893, 1082
HpyAV CCTTC 7 cut(s) 17, 300, 367, 400, 406, 789, 1125
HpyCH4III ACNGT 4 cut(s) 469, 783, 904, 1003
HpyCH4IV ACGT 2 cut(s) 73, 93
HpyF10VI GCNNNNNNNGC 9 cut(s) 135, 216, 231, 314, 796, 830, 857, 932, 1003
HpyF3I CTNAG 8 cut(s) 282, 318, 492, 547, 576, 608, 671, 800
HpySE526I ACGT 2 cut(s) 73, 93
Hsp92II CATG 4 cut(s) 100, 238, 941, 989
Kzo9I GATC 5 cut(s) 117, 262, 764, 889, 1099
LguI GCTCTTC 1 cut(s) 1157
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 7 cut(s) 163, 194, 777, 781, 823, 859, 938
LweI GCATC 5 cut(s) 125, 511, 570, 860, 1009
MaeI CTAG 2 cut(s) 260, 719
MaeII ACGT 2 cut(s) 73, 93
MalI GATC 5 cut(s) 119, 264, 766, 891, 1101
MboI GATC 5 cut(s) 117, 262, 764, 889, 1099
MflI RGATCY 1 cut(s) 1099
MlsI TGGCCA 1 cut(s) 833
MluCI AATT 1 cut(s) 724
MluNI TGGCCA 1 cut(s) 833
MlyI GAGTC 3 cut(s) 272, 684, 1144
Mox20I TGGCCA 1 cut(s) 833
Mph1103I ATGCAT 1 cut(s) 563
MroXI GAANNNNTTC 1 cut(s) 542
MscI TGGCCA 1 cut(s) 833
MseI TTAA 3 cut(s) 426, 461, 504
Msp20I TGGCCA 1 cut(s) 833
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 592
MvaI CCWGG 1 cut(s) 592
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 9 cut(s) 135, 216, 231, 314, 796, 830, 857, 932, 1003
NdeII GATC 5 cut(s) 117, 262, 764, 889, 1099
NlaIII CATG 4 cut(s) 100, 238, 941, 989
NlaIV GGNNCC 4 cut(s) 303, 393, 456, 1101
NsiI ATGCAT 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 1157
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 6 cut(s) 13, 527, 538, 587, 896, 989
PkrI GCNGC 8 cut(s) 175, 178, 209, 771, 792, 813, 874, 928
PleI GAGTC 3 cut(s) 272, 683, 1143
PpsI GAGTC 3 cut(s) 272, 683, 1143
Psp6I CCWGG 1 cut(s) 590
PspGI CCWGG 1 cut(s) 590
PspN4I GGNNCC 4 cut(s) 303, 393, 456, 1101
PspPI GGNCC 1 cut(s) 63
PsrI GAACNNNNNNTAC 2 cut(s) 426, 458
PstI CTGCAG 3 cut(s) 227, 401, 862
PstNI CAGNNNCTG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 1099
SapI GCTCTTC 1 cut(s) 1157
SaqAI TTAA 3 cut(s) 426, 461, 504
SatI GCNGC 8 cut(s) 174, 177, 208, 770, 791, 812, 873, 927
Sau3AI GATC 5 cut(s) 117, 262, 764, 889, 1099
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 3 cut(s) 272, 684, 1144
ScrFI CCNGG 1 cut(s) 592
SfaNI GCATC 5 cut(s) 125, 511, 570, 860, 1009
SfcI CTRYAG 3 cut(s) 223, 397, 858
SinI GGWCC 1 cut(s) 63
SmlI CTYRAG 3 cut(s) 140, 503, 930
SmoI CTYRAG 3 cut(s) 140, 503, 930
Sse9I AATT 1 cut(s) 724
SsiI CCGC 6 cut(s) 66, 173, 210, 308, 370, 473
SspMI CTAG 2 cut(s) 260, 719
StyD4I CCNGG 1 cut(s) 590
StyI CCWWGG 4 cut(s) 378, 834, 840, 846
TaaI ACNGT 4 cut(s) 469, 783, 904, 1003
TaiI ACGT 2 cut(s) 76, 96
TaqI TCGA 4 cut(s) 265, 731, 756, 1017
TasI AATT 1 cut(s) 724
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 6 cut(s) 13, 527, 538, 587, 896, 989
Tru1I TTAA 3 cut(s) 426, 461, 504
Tru9I TTAA 3 cut(s) 426, 461, 504
TscAI CASTG 2 cut(s) 232, 1008
TseI GCWGC 7 cut(s) 176, 207, 769, 790, 811, 872, 926
TspDTI ATGAA 4 cut(s) 17, 251, 717, 1134
TspRI CASTG 2 cut(s) 232, 1008
Vha464I CTTAAG 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 724
XbaI TCTAGA 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 2 cut(s) 260, 719
Zsp2I ATGCAT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.