RLG00000002348

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000951
Physical Location & Seq
Reverse (-)
32583040 .. 32587990
4951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002348

Sequence Viewer

Length: 1245 bp
ATGAGACGTGTGTTCTCATGCACTAGCATTATGGGGAGACGTCATGGCGCCAACAAGATCTGCCAGTCATGCCTCAAGAACAGGTACACGATAACAGATGAGGTGGTGGCTTCAAATGATTGGAAGTGCCCCAGATACTGTGGGAATTGCAACTGCAGTGTGTGCTTGAAGCGACAGGGCAAAGAACCTCGTAGTCGTAGTATGCTTGAACCCGAGAAGCCTTCAGACCAGAATTGTCCAAGGCAAATGGAGGGGAAGAAGACCAGAAAGAAGACGTTAAGGGCAAGCGGAAGTCCAAGGCTCCCAGAGCCCCATAGAGTGAAAGCAAAGCAGGAAGTACTTAGGGTTGAACTGTCTCTACCTTTGGGGGTGCCCTTAACAACCGTTGCGGGCATTGCACTAGATCCTCAGGATGTTGGACATGCCCTGCAATTTATAGAGTTCTGTGACGCCTTCCAAGAGGTTCTTGAGTTCAGCACAGCAGAAGCTGAATCTCTTCTTCGAGACCTGATGCGTCAAGAATACTCCTCAGTAGTTCTATTTCAAGCCAAACTCCTGTCTTTGATACATAAAAAGAAGATCGGAGAAGAGCATTCGGACGAAAATTCGTGGTTAGAAGTTTTGAAAAACTGCTTCTCCAAGTCTCAATTCCGACTAAGGGTATTGCCCACAGATGTGTTTAGTAGAGAAGTTGACGGATACGACTCTTTGCACTTCCCCTTGAAGCTTAGAGTGCCGGTTCAAAACAAAATATTCAATTTCTCCTCAAAAAAAAAAAAAAAAAACAAAATATCCAATTTTTCTAAAAAGAAAACAGAATATCCAGTTGATGACGGGTCGTCTCCAATCGGGTCGGAGTCTTATCGGACTCCGTCTCCGTTTAATCGGAGCAATTCGTCGATTTGCTTCCGACCGCGCAGAGCTGCTGCCCCTGCCCTAAAATCCCAACCCGGATTTCTCGATGACAAAAAACATAGTTACATAATAACATGCTTTTGGGGCTTTAAGAGATCTGTACTTGGGGTTCCAGTAAAAGCGACCATCAAAGAGGCAAACTATGAGTCTTTTGTAGTCCGAACTCTGGACAGGAGAACACTTTGGGAAATGCAGACACCACAGGTCATCAAACCTGACTTGCTCAAAAAGGGCTTTGAACTTGTCAACAGTTTCTGGCATATATGGGAGTGGCGTTGCAAGATCATTTTTGAGCCGGGTTCCACCACCCTTCTTGGTGTAATCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

415

Amino Acids

47.47

Weight (kDa)

9.61

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 17 - 65 1e-09 Zinc-finger domain of monoamine-oxidase A repressor R1
IspD PF01128 338 - 384 4.5e-08 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 43
AccB1I GGYRCC 2 cut(s) 47, 370
AccII CGCG 1 cut(s) 916
AciI CCGC 3 cut(s) 288, 389, 914
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 3 cut(s) 40, 48, 450
AfaI GTAC 3 cut(s) 86, 339, 1017
AfiI CCNNNNNNNGG 2 cut(s) 658, 1081
AflIII ACRYGT 1 cut(s) 7
AhdI GACNNNNNGTC 1 cut(s) 838
AjiI CACGTC 1 cut(s) 8
AjuI GAANNNNNNNTTGG 4 cut(s) 632, 664, 939, 971
AleI CACNNNNGTG 1 cut(s) 674
AluBI AGCT 3 cut(s) 488, 727, 923
AluI AGCT 3 cut(s) 488, 727, 923
Alw26I GTCTC 6 cut(s) 31, 360, 498, 648, 846, 879
AlwI GGATC 1 cut(s) 398
AlwNI CAGNNNCTG 3 cut(s) 138, 488, 1170
Ama87I CYCGRG 1 cut(s) 212
ApeKI GCWGC 2 cut(s) 923, 926
ApoI RAATTY 1 cut(s) 604
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 50, 918
AsuC2I CCSGG 2 cut(s) 951, 1212
AvaI CYCGRG 1 cut(s) 212
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 2 cut(s) 131, 375
BanI GGYRCC 2 cut(s) 47, 370
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 266, 278
BbvI GCAGC 2 cut(s) 910, 913
BccI CCATC 1 cut(s) 1049
BciVI GTATCC 1 cut(s) 692
BcnI CCSGG 2 cut(s) 951, 1212
BcoDI GTCTC 6 cut(s) 31, 360, 498, 648, 846, 879
BfaI CTAG 2 cut(s) 24, 401
BfmI CTRYAG 1 cut(s) 154
BfoI RGCGCY 1 cut(s) 51
BfuI GTATCC 1 cut(s) 692
BglII AGATCT 2 cut(s) 57, 1010
BisI GCNGC 2 cut(s) 924, 927
BlsI GCNGC 2 cut(s) 925, 928
BmcAI AGTACT 1 cut(s) 339
Bme1390I CCNGG 2 cut(s) 951, 1212
BmeRI GACNNNNNGTC 1 cut(s) 838
BmeT110I CYCGRG 1 cut(s) 212
BmgBI CACGTC 1 cut(s) 8
BmiI GGNNCC 5 cut(s) 49, 302, 372, 1026, 1216
BmrFI CCNGG 2 cut(s) 951, 1212
BmsI GCATC 1 cut(s) 501
BpiI GAAGAC 2 cut(s) 266, 278
BpuEI CTTGAG 2 cut(s) 59, 488
BpuMI CCSGG 2 cut(s) 951, 1212
BsaHI GRCGYC 3 cut(s) 40, 48, 450
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 239, 296
BsaXI ACNNNNNCTCC 4 cut(s) 620, 650, 859, 889
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 1081
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 3 cut(s) 64, 824, 1028
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 239, 296
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 658, 1081
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 2 cut(s) 422, 543
BseNI ACTGG 3 cut(s) 64, 824, 1028
BseRI GAGGAG 2 cut(s) 517, 754
BseSI GKGCMC 2 cut(s) 131, 375
BseXI GCAGC 2 cut(s) 910, 913
Bsh1236I CGCG 1 cut(s) 916
Bsh1285I CGRYCG 1 cut(s) 914
BshNI GGYRCC 2 cut(s) 47, 370
BsiEI CGRYCG 1 cut(s) 914
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 3 cut(s) 737, 951, 1211
BslI CCNNNNNNNGG 2 cut(s) 658, 1081
BsmAI GTCTC 6 cut(s) 31, 360, 498, 648, 846, 879
BsmBI CGTCTC 3 cut(s) 31, 846, 879
BsmI GAATGC 1 cut(s) 592
Bso31I GGTCTC 1 cut(s) 498
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 131, 312, 375
Bsp143I GATC 5 cut(s) 57, 403, 579, 1010, 1197
BspACI CCGC 3 cut(s) 288, 389, 914
BspCNI CTCAG 2 cut(s) 421, 542
BspFNI CGCG 1 cut(s) 916
BspLI GGNNCC 5 cut(s) 49, 302, 372, 1026, 1216
BspMAI CTGCAG 1 cut(s) 158
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 1 cut(s) 582
BspT107I GGYRCC 2 cut(s) 47, 370
BspTNI GGTCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 3 cut(s) 64, 824, 1028
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 2 cut(s) 239, 296
BssMI GATC 5 cut(s) 57, 403, 579, 1010, 1197
BssNI GRCGYC 3 cut(s) 40, 48, 450
BssT1I CCWWGG 2 cut(s) 239, 296
Bst4CI ACNGT 4 cut(s) 140, 354, 385, 1166
Bst6I CTCTTC 2 cut(s) 501, 582
BstACI GRCGYC 3 cut(s) 40, 48, 450
BstAPI GCANNNNNTGC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 286, 391
BstDEI CTNAG 5 cut(s) 341, 408, 529, 656, 728
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 916
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 918
BstKTI GATC 5 cut(s) 60, 406, 582, 1013, 1200
BstMAI GTCTC 6 cut(s) 31, 360, 498, 648, 846, 879
BstMBI GATC 5 cut(s) 57, 403, 579, 1010, 1197
BstMCI CGRYCG 1 cut(s) 914
BstMWI GCNNNNNNNGC 7 cut(s) 69, 162, 307, 395, 733, 932, 999
BstNSI RCATGY 2 cut(s) 425, 993
BstSCI CCNGG 2 cut(s) 949, 1210
BstSFI CTRYAG 1 cut(s) 154
BstSLI GKGCMC 2 cut(s) 131, 375
BstUI CGCG 1 cut(s) 916
BstV1I GCAGC 2 cut(s) 910, 913
BstV2I GAAGAC 2 cut(s) 266, 278
BstX2I RGATCY 3 cut(s) 57, 403, 1010
BstYI RGATCY 3 cut(s) 57, 403, 1010
Bsu36I CCTNAGG 1 cut(s) 408
BsuI GTATCC 1 cut(s) 692
BtrI CACGTC 1 cut(s) 8
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 163
Cac8I GCNNGC 2 cut(s) 286, 391
CaiI CAGNNNCTG 3 cut(s) 138, 488, 1170
CfoI GCGC 2 cut(s) 50, 918
Cfr10I RCCGGY 1 cut(s) 736
CseI GACGC 2 cut(s) 458, 503
Csp6I GTAC 3 cut(s) 85, 338, 1016
CspCI CAANNNNNGTGG 2 cut(s) 1210, 1245
CviAII CATG 5 cut(s) 18, 44, 69, 422, 990
CviQI GTAC 3 cut(s) 85, 338, 1016
DdeI CTNAG 5 cut(s) 341, 408, 529, 656, 728
DinI GGCGCC 1 cut(s) 49
DpnI GATC 5 cut(s) 59, 405, 581, 1012, 1199
DpnII GATC 5 cut(s) 57, 403, 579, 1010, 1197
DriI GACNNNNNGTC 1 cut(s) 838
Eam1104I CTCTTC 2 cut(s) 501, 582
Eam1105I GACNNNNNGTC 1 cut(s) 838
EarI CTCTTC 2 cut(s) 501, 582
Eco130I CCWWGG 2 cut(s) 239, 296
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 239, 296
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
ErhI CCWWGG 2 cut(s) 239, 296
Esp3I CGTCTC 3 cut(s) 31, 846, 879
FaeI CATG 5 cut(s) 21, 47, 72, 425, 993
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FatI CATG 5 cut(s) 17, 43, 68, 421, 989
FauI CCCGC 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 924, 927
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 924, 927
FspBI CTAG 2 cut(s) 24, 401
GlaI GCGC 2 cut(s) 49, 917
GluI GCNGC 2 cut(s) 924, 927
HaeII RGCGCY 1 cut(s) 51
HapII CCGG 3 cut(s) 737, 951, 1211
HgaI GACGC 2 cut(s) 458, 503
HhaI GCGC 2 cut(s) 50, 918
Hin1I GRCGYC 3 cut(s) 40, 48, 450
Hin1II CATG 5 cut(s) 21, 47, 72, 425, 993
Hin6I GCGC 2 cut(s) 48, 916
HinP1I GCGC 2 cut(s) 48, 916
HincII GTYRAC 2 cut(s) 694, 1162
HindII GTYRAC 2 cut(s) 694, 1162
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 5 cut(s) 491, 704, 857, 868, 1061
HpaII CCGG 3 cut(s) 737, 951, 1211
Hpy166II GTNNAC 3 cut(s) 87, 694, 1162
Hpy188I TCNGA 9 cut(s) 226, 584, 598, 653, 856, 867, 888, 911, 1076
Hpy188III TCNNGA 7 cut(s) 76, 410, 467, 503, 518, 959, 1082
Hpy8I GTNNAC 3 cut(s) 87, 694, 1162
Hpy99I CGWCG 1 cut(s) 901
HpyAV CCTTC 3 cut(s) 231, 463, 1235
HpyCH4III ACNGT 4 cut(s) 140, 354, 385, 1166
HpyCH4IV ACGT 3 cut(s) 7, 40, 275
HpyCH4V TGCA 8 cut(s) 21, 150, 156, 398, 430, 712, 1108, 1194
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 162, 307, 395, 733, 932, 999
HpyF3I CTNAG 5 cut(s) 341, 408, 529, 656, 728
HpySE526I ACGT 3 cut(s) 7, 40, 275
Hsp92I GRCGYC 3 cut(s) 40, 48, 450
Hsp92II CATG 5 cut(s) 21, 47, 72, 425, 993
HspAI GCGC 2 cut(s) 48, 916
KasI GGCGCC 1 cut(s) 47
Kzo9I GATC 5 cut(s) 57, 403, 579, 1010, 1197
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 2 cut(s) 306, 888
Lsp1109I GCAGC 2 cut(s) 910, 913
LweI GCATC 1 cut(s) 501
MaeI CTAG 2 cut(s) 24, 401
MaeII ACGT 3 cut(s) 7, 40, 275
MaeIII GTNAC 2 cut(s) 446, 977
MalI GATC 5 cut(s) 59, 405, 581, 1012, 1199
MboI GATC 5 cut(s) 57, 403, 579, 1010, 1197
MboII GAAGA 7 cut(s) 268, 271, 283, 488, 491, 589, 599
MflI RGATCY 3 cut(s) 57, 403, 1010
MhlI GDGCHC 3 cut(s) 131, 312, 375
MluCI AATT 8 cut(s) 145, 232, 431, 604, 647, 757, 796, 892
Mly113I GGCGCC 1 cut(s) 48
MlyI GAGTC 4 cut(s) 698, 862, 866, 1070
MmeI TCCRAC 4 cut(s) 397, 676, 834, 934
MnlI CCTC 9 cut(s) 83, 94, 198, 244, 417, 454, 538, 775, 1042
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 4 cut(s) 278, 377, 882, 1005
MslI CAYNNNNRTG 1 cut(s) 674
MspI CCGG 3 cut(s) 737, 951, 1211
MspR9I CCNGG 2 cut(s) 951, 1212
Mva1269I GAATGC 1 cut(s) 592
MvnI CGCG 1 cut(s) 916
MwoI GCNNNNNNNGC 7 cut(s) 69, 162, 307, 395, 733, 932, 999
NarI GGCGCC 1 cut(s) 48
NciI CCSGG 2 cut(s) 951, 1212
NdeII GATC 5 cut(s) 57, 403, 579, 1010, 1197
NlaIII CATG 5 cut(s) 21, 47, 72, 425, 993
NlaIV GGNNCC 5 cut(s) 49, 302, 372, 1026, 1216
NmuCI GTSAC 1 cut(s) 446
NspI RCATGY 2 cut(s) 425, 993
OliI CACNNNNGTG 1 cut(s) 674
PciSI GCTCTTC 1 cut(s) 582
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 1 cut(s) 491
PflFI GACNNNGTC 1 cut(s) 871
PkrI GCNGC 2 cut(s) 925, 928
PleI GAGTC 4 cut(s) 698, 862, 865, 1069
PluTI GGCGCC 1 cut(s) 51
PpsI GAGTC 4 cut(s) 698, 862, 865, 1069
PspN4I GGNNCC 5 cut(s) 49, 302, 372, 1026, 1216
PsrI GAACNNNNNNTAC 4 cut(s) 342, 374, 1008, 1040
PstI CTGCAG 1 cut(s) 158
PstNI CAGNNNCTG 3 cut(s) 138, 488, 1170
PsuI RGATCY 3 cut(s) 57, 403, 1010
PsyI GACNNNGTC 1 cut(s) 871
RsaI GTAC 3 cut(s) 86, 339, 1017
RsaNI GTAC 3 cut(s) 85, 338, 1016
RseI CAYNNNNRTG 1 cut(s) 674
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 4 cut(s) 278, 377, 882, 1005
SatI GCNGC 2 cut(s) 924, 927
Sau3AI GATC 5 cut(s) 57, 403, 579, 1010, 1197
ScaI AGTACT 1 cut(s) 339
SchI GAGTC 4 cut(s) 698, 862, 866, 1070
ScrFI CCNGG 2 cut(s) 951, 1212
SduI GDGCHC 3 cut(s) 131, 312, 375
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 1 cut(s) 154
SfoI GGCGCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 2 cut(s) 74, 467
SmoI CTYRAG 2 cut(s) 74, 467
Sse9I AATT 8 cut(s) 145, 232, 431, 604, 647, 757, 796, 892
SsiI CCGC 3 cut(s) 288, 389, 914
SspDI GGCGCC 1 cut(s) 47
SspI AATATT 1 cut(s) 753
SspMI CTAG 2 cut(s) 24, 401
StyD4I CCNGG 2 cut(s) 949, 1210
StyI CCWWGG 2 cut(s) 239, 296
TaaI ACNGT 4 cut(s) 140, 354, 385, 1166
TaiI ACGT 3 cut(s) 10, 43, 278
TaqI TCGA 3 cut(s) 502, 899, 960
TasI AATT 8 cut(s) 145, 232, 431, 604, 647, 757, 796, 892
TatI WGTACW 2 cut(s) 337, 1015
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 4 cut(s) 278, 377, 882, 1005
Tru9I TTAA 4 cut(s) 278, 377, 882, 1005
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 2 cut(s) 923, 926
Tsp45I GTSAC 1 cut(s) 446
TspGWI ACGGA 3 cut(s) 711, 861, 867
TspRI CASTG 1 cut(s) 163
Tth111I GACNNNGTC 1 cut(s) 871
XapI RAATTY 1 cut(s) 604
XceI RCATGY 2 cut(s) 425, 993
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 2 cut(s) 24, 401
ZraI GACGTC 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.