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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 88, 134 |
| AciI | CCGC | 6 cut(s) | 53, 86, 134, 221, 238, 265 |
| AcsI | RAATTY | 2 cut(s) | 215, 243 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 165 |
| AgsI | TTSAA | 4 cut(s) | 340, 347, 367, 386 |
| Alw26I | GTCTC | 1 cut(s) | 9 |
| ApeKI | GCWGC | 1 cut(s) | 232 |
| ApoI | RAATTY | 2 cut(s) | 215, 243 |
| AspLEI | GCGC | 2 cut(s) | 90, 237 |
| BbsI | GAAGAC | 1 cut(s) | 374 |
| BbvI | GCAGC | 1 cut(s) | 244 |
| BcoDI | GTCTC | 1 cut(s) | 9 |
| BfaI | CTAG | 2 cut(s) | 170, 272 |
| BfoI | RGCGCY | 1 cut(s) | 238 |
| BisI | GCNGC | 4 cut(s) | 53, 86, 233, 238 |
| BlsI | GCNGC | 4 cut(s) | 54, 87, 234, 239 |
| BpiI | GAAGAC | 1 cut(s) | 374 |
| BpmI | CTGGAG | 1 cut(s) | 12 |
| BsaJI | CCNNGG | 1 cut(s) | 42 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 165 |
| Bse118I | RCCGGY | 1 cut(s) | 67 |
| Bse1I | ACTGG | 2 cut(s) | 29, 357 |
| BseDI | CCNNGG | 1 cut(s) | 42 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 165 |
| BseMII | CTCAG | 2 cut(s) | 188, 304 |
| BseNI | ACTGG | 2 cut(s) | 29, 357 |
| BseRI | GAGGAG | 2 cut(s) | 214, 239 |
| BseXI | GCAGC | 1 cut(s) | 244 |
| Bsh1236I | CGCG | 2 cut(s) | 88, 134 |
| BsiSI | CCGG | 1 cut(s) | 68 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 165 |
| BsmAI | GTCTC | 1 cut(s) | 9 |
| BsmI | GAATGC | 1 cut(s) | 306 |
| Bsp143I | GATC | 1 cut(s) | 13 |
| BspACI | CCGC | 6 cut(s) | 53, 86, 134, 221, 238, 265 |
| BspCNI | CTCAG | 2 cut(s) | 189, 303 |
| BspFNI | CGCG | 2 cut(s) | 88, 134 |
| BsrFI | RCCGGY | 1 cut(s) | 67 |
| BsrI | ACTGG | 2 cut(s) | 29, 357 |
| BssAI | RCCGGY | 1 cut(s) | 67 |
| BssECI | CCNNGG | 1 cut(s) | 42 |
| BssMI | GATC | 1 cut(s) | 13 |
| BssT1I | CCWWGG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 1 cut(s) | 380 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 320 |
| BstDEI | CTNAG | 2 cut(s) | 197, 290 |
| BstFNI | CGCG | 2 cut(s) | 88, 134 |
| BstH2I | RGCGCY | 1 cut(s) | 238 |
| BstHHI | GCGC | 2 cut(s) | 90, 237 |
| BstKTI | GATC | 1 cut(s) | 16 |
| BstMAI | GTCTC | 1 cut(s) | 9 |
| BstMBI | GATC | 1 cut(s) | 13 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 246, 320 |
| BstUI | CGCG | 2 cut(s) | 88, 134 |
| BstV1I | GCAGC | 1 cut(s) | 244 |
| BstV2I | GAAGAC | 1 cut(s) | 374 |
| BtgZI | GCGATG | 2 cut(s) | 48, 115 |
| BtsIMutI | CAGTG | 1 cut(s) | 36 |
| CfoI | GCGC | 2 cut(s) | 90, 237 |
| Cfr10I | RCCGGY | 1 cut(s) | 67 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 24, 59 |
| CviAII | CATG | 1 cut(s) | 315 |
| CviJI | RGCY | 4 cut(s) | 23, 169, 194, 281 |
| CviKI_1 | RGCY | 4 cut(s) | 23, 169, 194, 281 |
| DdeI | CTNAG | 2 cut(s) | 197, 290 |
| DpnI | GATC | 1 cut(s) | 15 |
| DpnII | GATC | 1 cut(s) | 13 |
| Eco130I | CCWWGG | 1 cut(s) | 42 |
| EcoT14I | CCWWGG | 1 cut(s) | 42 |
| ErhI | CCWWGG | 1 cut(s) | 42 |
| FaeI | CATG | 1 cut(s) | 318 |
| FaiI | YATR | 2 cut(s) | 263, 316 |
| FatI | CATG | 1 cut(s) | 314 |
| FauI | CCCGC | 1 cut(s) | 258 |
| Fnu4HI | GCNGC | 4 cut(s) | 53, 86, 233, 238 |
| Fsp4HI | GCNGC | 4 cut(s) | 53, 86, 233, 238 |
| FspBI | CTAG | 2 cut(s) | 170, 272 |
| GlaI | GCGC | 2 cut(s) | 89, 236 |
| GluI | GCNGC | 4 cut(s) | 53, 86, 233, 238 |
| GsuI | CTGGAG | 1 cut(s) | 12 |
| HaeII | RGCGCY | 1 cut(s) | 238 |
| HapII | CCGG | 1 cut(s) | 68 |
| HhaI | GCGC | 2 cut(s) | 90, 237 |
| Hin1II | CATG | 1 cut(s) | 318 |
| Hin6I | GCGC | 2 cut(s) | 88, 235 |
| HinP1I | GCGC | 2 cut(s) | 88, 235 |
| HinfI | GANTC | 1 cut(s) | 173 |
| HpaII | CCGG | 1 cut(s) | 68 |
| Hpy188I | TCNGA | 3 cut(s) | 80, 178, 198 |
| HpyCH4III | ACNGT | 1 cut(s) | 380 |
| HpyCH4IV | ACGT | 1 cut(s) | 10 |
| HpyCH4V | TGCA | 2 cut(s) | 232, 304 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 246, 320 |
| HpyF3I | CTNAG | 2 cut(s) | 197, 290 |
| HpySE526I | ACGT | 1 cut(s) | 10 |
| Hsp92II | CATG | 1 cut(s) | 318 |
| HspAI | GCGC | 2 cut(s) | 88, 235 |
| Kzo9I | GATC | 1 cut(s) | 13 |
| LpnPI | CCDG | 5 cut(s) | 42, 81, 277, 338, 343 |
| Lsp1109I | GCAGC | 1 cut(s) | 244 |
| MaeI | CTAG | 2 cut(s) | 170, 272 |
| MaeII | ACGT | 1 cut(s) | 10 |
| MalI | GATC | 1 cut(s) | 15 |
| MboI | GATC | 1 cut(s) | 13 |
| MboII | GAAGA | 1 cut(s) | 374 |
| MluCI | AATT | 2 cut(s) | 215, 243 |
| MnlI | CCTC | 6 cut(s) | 70, 109, 184, 192, 217, 326 |
| MseI | TTAA | 2 cut(s) | 138, 254 |
| MslI | CAYNNNNRTG | 1 cut(s) | 254 |
| MspI | CCGG | 1 cut(s) | 68 |
| Mva1269I | GAATGC | 1 cut(s) | 306 |
| MvnI | CGCG | 2 cut(s) | 88, 134 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 246, 320 |
| NdeII | GATC | 1 cut(s) | 13 |
| NlaIII | CATG | 1 cut(s) | 318 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 120 |
| PctI | GAATGC | 1 cut(s) | 306 |
| PfeI | GAWTC | 1 cut(s) | 173 |
| PkrI | GCNGC | 4 cut(s) | 54, 87, 234, 239 |
| RseI | CAYNNNNRTG | 1 cut(s) | 254 |
| SaqAI | TTAA | 2 cut(s) | 138, 254 |
| SatI | GCNGC | 4 cut(s) | 53, 86, 233, 238 |
| Sau3AI | GATC | 1 cut(s) | 13 |
| SetI | ASST | 3 cut(s) | 13, 296, 337 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 254 |
| Sse9I | AATT | 2 cut(s) | 215, 243 |
| SsiI | CCGC | 6 cut(s) | 53, 86, 134, 221, 238, 265 |
| SspMI | CTAG | 2 cut(s) | 170, 272 |
| StyI | CCWWGG | 1 cut(s) | 42 |
| TaaI | ACNGT | 1 cut(s) | 380 |
| TaiI | ACGT | 1 cut(s) | 13 |
| TaqI | TCGA | 1 cut(s) | 123 |
| TasI | AATT | 2 cut(s) | 215, 243 |
| TauI | GCSGC | 3 cut(s) | 55, 88, 240 |
| TfiI | GAWTC | 1 cut(s) | 173 |
| Tru1I | TTAA | 2 cut(s) | 138, 254 |
| Tru9I | TTAA | 2 cut(s) | 138, 254 |
| TscAI | CASTG | 1 cut(s) | 36 |
| TseI | GCWGC | 1 cut(s) | 232 |
| TspRI | CASTG | 1 cut(s) | 36 |
| XapI | RAATTY | 2 cut(s) | 215, 243 |
| XspI | CTAG | 2 cut(s) | 170, 272 |