Rh7BG316200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000951
Physical Location & Seq
Forward (+)
30540200 .. 30554326
14127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG316200.1

Sequence Viewer

Length: 579 bp
ATGGGCCTCCCAGTGAAGCTTGAGAGTTCTTCCTTGTGTGTGGTACCCTCAGATTCACAGAAAAGTACTCCACCACCACTGGAATCGCAACCATCCTCATCGCCGACGCCCCCGCACAAAGGCAGAGGCAGAGGCAGAGCCACCCGAAAATGCCAAATCGGTTCAGTCGACGACGATGTCAGGGTTAATCTAGAAAGGGTTTTCGCCCTATCTTGGCCAAACTCTGACTTTATTCTCGAGGCCTTGAGGATAGAACGACAACAATTCGCCGAGGAATTGCAACGCCGTAAGATTCGGATTCTAATGTCTATGCGATTGTTGAGTCTCTCCTTGGCAGTGATATTAGCAAGATTGTTGCAAGCTGGAACAATAGCAAATTCACGAAAAATGGTTGAAGAATTCAGAGTTAAGAAGATGGTTGAAGATGTTAGAGTTGATAACATGGTTGAAGATGGTGGAGTAAAGATGGTTGAATTTGATCATAGGAATGAAGATAAAGCTTCGGGATTGAGTAGGGGTGAAGATGATGAACTGGAGAAGGGTGAAGATGATCAAGATTATGATAGCAGCTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.54

Weight (kDa)

5.4

Isoelectric Point (pI)

62.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
Acc65I GGTACC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 168
AciI CCGC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 3 cut(s) 376, 398, 473
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 45, 67
AfiI CCNNNNNNNGG 2 cut(s) 119, 213
AgsI TTSAA 4 cut(s) 395, 422, 449, 473
AluBI AGCT 4 cut(s) 19, 362, 500, 570
AluI AGCT 4 cut(s) 19, 362, 500, 570
Alw26I GTCTC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 3 cut(s) 4, 215, 240
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 3 cut(s) 376, 398, 473
Asp718I GGTACC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 530, 554
AvaI CYCGRG 1 cut(s) 236
BalI TGGCCA 1 cut(s) 217
BanI GGYRCC 1 cut(s) 43
BccI CCATC 4 cut(s) 100, 409, 446, 460
BceAI ACGGC 1 cut(s) 270
BclI TGATCA 2 cut(s) 478, 550
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 568
BlsI GCNGC 1 cut(s) 569
BmcAI AGTACT 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 2 cut(s) 41, 265
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 270, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 213
Bse1I ACTGG 3 cut(s) 11, 84, 537
BseDI CCNNGG 2 cut(s) 270, 330
BseGI GGATG 1 cut(s) 92
BseLI CCNNNNNNNGG 2 cut(s) 119, 213
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 3 cut(s) 11, 84, 537
BshFI GGCC 3 cut(s) 6, 217, 242
BshNI GGYRCC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 236
BslI CCNNNNNNNGG 2 cut(s) 119, 213
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 3 cut(s) 6, 217, 242
BsoBI CYCGRG 1 cut(s) 236
Bsp143I GATC 2 cut(s) 478, 550
BspACI CCGC 1 cut(s) 113
BspANI GGCC 3 cut(s) 6, 217, 242
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 43
BsrI ACTGG 3 cut(s) 11, 84, 537
BssECI CCNNGG 2 cut(s) 270, 330
BssMI GATC 2 cut(s) 478, 550
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 92
BstKTI GATC 2 cut(s) 481, 553
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 2 cut(s) 478, 550
BsuRI GGCC 3 cut(s) 6, 217, 242
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 92
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 18, 77, 342
Cac8I GCNNGC 1 cut(s) 360
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 44, 66
CviAII CATG 1 cut(s) 442
CviJI RGCY 8 cut(s) 6, 19, 140, 217, 242, 362, 500, 570
CviKI_1 RGCY 8 cut(s) 6, 19, 140, 217, 242, 362, 500, 570
CviQI GTAC 2 cut(s) 44, 66
DdeI CTNAG 1 cut(s) 49
DpnI GATC 2 cut(s) 480, 552
DpnII GATC 2 cut(s) 478, 550
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 330
Eco147I AGGCCT 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 1 cut(s) 445
FaiI YATR 5 cut(s) 311, 443, 483, 561, 577
FatI CATG 1 cut(s) 441
FauI CCCGC 1 cut(s) 120
FbaI TGATCA 2 cut(s) 478, 550
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 568
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 1 cut(s) 568
FspBI CTAG 1 cut(s) 191
GluI GCNGC 1 cut(s) 568
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 3 cut(s) 6, 217, 242
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 1 cut(s) 445
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 17, 498
HinfI GANTC 5 cut(s) 53, 83, 292, 298, 322
HphI GGTGA 2 cut(s) 530, 554
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 4 cut(s) 52, 226, 297, 404
Hpy188III TCNNGA 5 cut(s) 191, 236, 381, 504, 554
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 3 cut(s) 109, 173, 176
HpyAV CCTTC 1 cut(s) 532
HpyCH4V TGCA 2 cut(s) 280, 358
HpyF3I CTNAG 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 1 cut(s) 445
KpnI GGTACC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 478, 550
Kzo9I GATC 2 cut(s) 478, 550
LmnI GCTCC 1 cut(s) 575
LpnPI CCDG 5 cut(s) 24, 65, 166, 348, 518
MaeI CTAG 1 cut(s) 191
MalI GATC 2 cut(s) 480, 552
MboI GATC 2 cut(s) 478, 550
MboII GAAGA 8 cut(s) 21, 407, 424, 434, 461, 503, 533, 557
MlsI TGGCCA 1 cut(s) 217
MluCI AATT 5 cut(s) 263, 275, 376, 398, 473
MluNI TGGCCA 1 cut(s) 217
MlyI GAGTC 1 cut(s) 331
MnlI CCTC 8 cut(s) 17, 58, 106, 119, 125, 232, 240, 265
Mox20I TGGCCA 1 cut(s) 217
MscI TGGCCA 1 cut(s) 217
MseI TTAA 2 cut(s) 186, 408
MslI CAYNNNNRTG 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 217
NdeII GATC 2 cut(s) 478, 550
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 1 cut(s) 45
NmeAIII GCCGAG 1 cut(s) 295
PaeR7I CTCGAG 1 cut(s) 236
PceI AGGCCT 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 4 cut(s) 53, 83, 292, 298
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 1 cut(s) 569
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 45, 67
RsaNI GTAC 2 cut(s) 44, 66
RseI CAYNNNNRTG 1 cut(s) 486
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 186, 408
SatI GCNGC 1 cut(s) 568
Sau3AI GATC 2 cut(s) 478, 550
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 1 cut(s) 331
SetI ASST 4 cut(s) 21, 364, 502, 572
Sfr274I CTCGAG 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 20, 236, 244
SmoI CTYRAG 3 cut(s) 20, 236, 244
Sse9I AATT 5 cut(s) 263, 275, 376, 398, 473
SseBI AGGCCT 1 cut(s) 242
SsiI CCGC 1 cut(s) 113
SspMI CTAG 1 cut(s) 191
StuI AGGCCT 1 cut(s) 242
StyI CCWWGG 1 cut(s) 330
TaqI TCGA 2 cut(s) 168, 237
TasI AATT 5 cut(s) 263, 275, 376, 398, 473
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 4 cut(s) 53, 83, 292, 298
Tru1I TTAA 2 cut(s) 186, 408
Tru9I TTAA 2 cut(s) 186, 408
TscAI CASTG 3 cut(s) 18, 84, 342
TseI GCWGC 1 cut(s) 567
TspDTI ATGAA 2 cut(s) 504, 543
TspRI CASTG 3 cut(s) 18, 84, 342
Tth111I GACNNNGTC 1 cut(s) 176
XapI RAATTY 3 cut(s) 376, 398, 473
XbaI TCTAGA 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 1 cut(s) 191
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.