Rorug07G0290400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000951
Physical Location & Seq
Reverse (-)
28300486 .. 28305401
4916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0290400.1

Sequence Viewer

Length: 669 bp
ATGGGAGAGAAAGATACAGTGTGTGTAACTGGTGCTGGAGGCTTTGTGGCATCTTGGGTCGTCAAGCTGCTTCTCTCTCAGGGATACAAAGTTCATGGCACTGCTAGAGACCCAGGTGATGAGAAGAATGCCCATTTGAAGAAATTGGAAAACGCTTCTGAGAATTTGCAACTGTTCAAGGCAGACTTGTTCGACTTTGAAGGTCTTTGTGCTGCCATTTCTGGGTGTTCTGGTGTTTTCCATGTTGCCTGCCCTGTTCCATCGGGCGAAGTTTCTAACTCTGATGAGAGAGAATTTATCGAACCTGTTGTAAGGGGCAGACGGAACGTACTAGATGCATGTATCAAGGCAAAAGTAAAGAGGGTCGTGGTGCTGTTATCTGCTGGTGCTGTTATATACAACCCAAAATGGCCCAAGGATAAACCCATGGATGAAGAATGTTGGTCTGACCCAGAGTATTGCAAGAAAGCTAAGACCCAGTTTAGCAACTATTGTCTTGCCAAGACAATGGCAGAAACTGAAGCTTTGGAGTATGCAAAGAAAAGTGAATTGAGAATCATTACTGTTTGTCCATCCTTTGTTTTTGGTCCAATGCTGCAATCAACATTAAATGCCAGTAACTCATTGCTCATAACTCTTCTAAAAGATGGACTGGCCGGAATCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.2

Weight (kDa)

6.81

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 124 1.7e-09 NmrA-like family
Epimerase PF01370 7 - 202 2.6e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 9 - 207 1.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 9 - 192 1.9e-13 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 11 - 191 1.6e-13 NAD(P)H-binding
NAD_binding_4 PF07993 79 - 207 5.4e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 2 cut(s) 163, 293
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 3 cut(s) 139, 178, 200
AjnI CCWGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
AluBI AGCT 3 cut(s) 67, 470, 524
AluI AGCT 3 cut(s) 67, 470, 524
Alw26I GTCTC 1 cut(s) 102
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 2 cut(s) 410, 654
ApeKI GCWGC 3 cut(s) 67, 212, 595
ApoI RAATTY 2 cut(s) 163, 293
AspS9I GGNCC 2 cut(s) 411, 587
AsuHPI GGTGA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 54, 199, 582
BccI CCATC 3 cut(s) 268, 580, 641
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 2 cut(s) 105, 332
BfuI GTATCC 1 cut(s) 77
BisI GCNGC 3 cut(s) 68, 213, 596
BlsI GCNGC 3 cut(s) 69, 214, 597
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 411, 587
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 2 cut(s) 59, 325
BmuI ACTGGG 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 112, 414, 426
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 4 cut(s) 34, 478, 615, 657
Bse3DI GCAATG 1 cut(s) 623
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 112, 414, 426
BseGI GGATG 2 cut(s) 436, 572
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 92, 150
BseNI ACTGG 4 cut(s) 34, 478, 615, 657
BseXI GCAGC 3 cut(s) 54, 199, 582
BshFI GGCC 2 cut(s) 412, 656
BsiSI CCGG 1 cut(s) 657
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 2 cut(s) 412, 656
Bso31I GGTCTC 1 cut(s) 102
Bsp19I CCATGG 1 cut(s) 426
BspANI GGCC 2 cut(s) 412, 656
BspCNI CTCAG 2 cut(s) 91, 151
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 623
BsrI ACTGG 4 cut(s) 34, 478, 615, 657
BssECI CCNNGG 3 cut(s) 112, 414, 426
BssT1I CCWWGG 2 cut(s) 414, 426
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 19, 174, 565
Bst6I CTCTTC 1 cut(s) 642
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 3 cut(s) 78, 159, 471
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 2 cut(s) 436, 572
BstMAI GTCTC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 54, 199, 582
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuI GTATCC 1 cut(s) 77
BsuRI GGCC 2 cut(s) 412, 656
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 2 cut(s) 436, 572
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 2 cut(s) 24, 99
Cac8I GCNNGC 1 cut(s) 250
CaiI CAGNNNCTG 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 411, 587
Csp6I GTAC 1 cut(s) 329
CviAII CATG 4 cut(s) 95, 242, 339, 427
CviJI RGCY 6 cut(s) 42, 67, 412, 470, 524, 656
CviKI_1 RGCY 6 cut(s) 42, 67, 412, 470, 524, 656
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 3 cut(s) 78, 159, 471
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 2 cut(s) 414, 426
Eco31I GGTCTC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 414, 426
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 414, 426
FaeI CATG 4 cut(s) 98, 245, 342, 430
FaiI YATR 8 cut(s) 96, 243, 340, 395, 397, 428, 534, 632
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 4 cut(s) 94, 241, 338, 426
Fnu4HI GCNGC 3 cut(s) 68, 213, 596
FokI GGATG 2 cut(s) 443, 559
Fsp4HI GCNGC 3 cut(s) 68, 213, 596
FspBI CTAG 2 cut(s) 105, 332
GluI GCNGC 3 cut(s) 68, 213, 596
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 2 cut(s) 412, 656
HapII CCGG 1 cut(s) 657
Hin1II CATG 4 cut(s) 98, 245, 342, 430
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 2 cut(s) 555, 660
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 128
Hpy188I TCNGA 3 cut(s) 160, 283, 448
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 3 cut(s) 19, 174, 565
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 5 cut(s) 169, 338, 462, 536, 598
HpyF3I CTNAG 3 cut(s) 78, 159, 471
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 4 cut(s) 98, 245, 342, 430
Lsp1109I GCAGC 3 cut(s) 54, 199, 582
LweI GCATC 2 cut(s) 59, 325
MaeI CTAG 2 cut(s) 105, 332
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 25, 617
MboII GAAGA 4 cut(s) 136, 151, 446, 629
MluCI AATT 4 cut(s) 143, 163, 293, 548
MnlI CCTC 2 cut(s) 32, 354
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 1 cut(s) 608
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 114
NcoI CCATGG 1 cut(s) 426
NlaIII CATG 4 cut(s) 98, 245, 342, 430
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 342
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 555, 660
PkrI GCNGC 3 cut(s) 69, 214, 597
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 2 cut(s) 411, 587
PstNI CAGNNNCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 3 cut(s) 68, 213, 596
Sau96I GGNCC 2 cut(s) 411, 587
ScrFI CCNGG 1 cut(s) 114
SetI ASST 7 cut(s) 69, 118, 205, 307, 330, 472, 526
SfaNI GCATC 2 cut(s) 59, 325
SinI GGWCC 1 cut(s) 587
Sse9I AATT 4 cut(s) 143, 163, 293, 548
SspMI CTAG 2 cut(s) 105, 332
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 2 cut(s) 414, 426
TaaI ACNGT 3 cut(s) 19, 174, 565
TaiI ACGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 192, 300
TasI AATT 4 cut(s) 143, 163, 293, 548
TfiI GAWTC 2 cut(s) 555, 660
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 24, 106
TseI GCWGC 3 cut(s) 67, 212, 595
TspDTI ATGAA 2 cut(s) 83, 447
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 2 cut(s) 24, 106
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 163, 293
XceI RCATGY 1 cut(s) 342
XspI CTAG 2 cut(s) 105, 332
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.