RLG00000002337

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000951
Physical Location & Seq
Forward (+)
32334788 .. 32337320
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002337

Sequence Viewer

Length: 1188 bp
ATGGCCGGAGAAGGAGTATTCTGTCACCAGTGCCGCCAAAGGACCATGAGACGTGTGTTCTCATGCACTAGCATTATGGGGAGACATCATGGCGCCAACAAGATCTGCCAGTCATGCCTCAAGAACAGGTACACGATAACAGATGAGGTGGTGGCTTCAGATGATCGGAAGTGCCCCAGATGCTGTGGGAATTGCAACTGCAGTGTGTGCTTGAAGCGACAAGGCAAAGAACCTCTCAGTCGTAGTGTGCTTGAACCCGAGAAGCCTTCAGACCAGAATTGTCTGAGGCAAATGGAGGGGAAGAAGACCAGAAAGAAGACGTTAAGGGCAAGCGGAAGTCCAAGGCTCCCAGAGCCCCGTAGAGTGAAAGCAAAGGAGGAAGTACTTAGGGTTGAACTGTCTCTACCTTTGGGGGTGTCCTTAACAACCGTTGCGGGCATTGCACTAGATCCTCAAGATGTTGGACATGCCCTACAATTTATACACACAGCAGAAGCTGAATCTCTTCTCCGAGACTTGATGCGTCAAGAATACTCCTCAGTAGTTCTATTTCAAGCCAAACTCCTGTCTTTGATACATAAAACGAAGATCGGAGAAGAGCATTCGGACAAAAATTCATGGTTAGAAGTTTTGAAAAACTGCTTCTCCAAGTCTCAATTCCGACTAAGGGTATTGCCCACAGATTTGTTTAGTAGAGAAGTTGATGGATACGACTCTTTGCACTTCTCCTTGAAGCTTAGAGTCGTAACTTTCCTTTGTGATGAGGCTCTTGACACTACATCACTGAAAAATTTCATAGACCAAAAAGTAAATGTAATTTCTGAAAGAAAGAGAGAAGCAAATAAAAGATTTTCGGTTGCAAATGATAAGGAGATAGGTCACAAGAGGCAGTTAAAAGTTGAGGTGGCCATGGCCAAGACCATTAGTGCAGGAGACGATGCTGCTTCGAACTTTAGATTGCGAATCTATTGGAAGCAACAGCCTGATTCTTTTAGAACAGAGCCTTATCTGTTCGACACTGATGGTCATGCCTATTGGAAATTGAAAGGCTATAATGATGGAGACAATGTGCTACTTCAAGATATGGGAACTGGGGATCCATTTGTATCTTCTGATGAACAATGGTTCTCGTATGGAGCAGAGACAAAAGAAGCAGCTGAGAATTACTACTCTTCTACAAGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

396

Amino Acids

45.03

Weight (kDa)

8.88

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 4 - 79 7.5e-15 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 92
AciI CCGC 3 cut(s) 34, 333, 434
AclWI GGATC 3 cut(s) 443, 1091, 1104
AcoI YGGCCR 3 cut(s) 3, 906, 912
AcsI RAATTY 2 cut(s) 613, 790
AcuI CTGAAG 2 cut(s) 141, 252
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 131, 384
AfiI CCNNNNNNNGG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 8 cut(s) 214, 254, 395, 554, 634, 733, 1045, 1079
AjiI CACGTC 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AloI GAACNNNNNNTCC 2 cut(s) 1081, 1113
AluBI AGCT 3 cut(s) 497, 736, 1157
AluI AGCT 3 cut(s) 497, 736, 1157
Alw26I GTCTC 8 cut(s) 43, 76, 405, 507, 657, 927, 1056, 1136
AlwI GGATC 3 cut(s) 443, 1091, 1104
AlwNI CAGNNNCTG 2 cut(s) 183, 497
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 3 cut(s) 3, 906, 912
ApeKI GCWGC 2 cut(s) 941, 1154
ApoI RAATTY 2 cut(s) 613, 790
Asp700I GAANNNNTTC 2 cut(s) 504, 791
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 17
AsuII TTCGAA 1 cut(s) 947
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 176
BalI TGGCCA 2 cut(s) 908, 914
BamHI GGATCC 1 cut(s) 1096
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 311, 323
BbvI GCAGC 2 cut(s) 928, 1166
BccI CCATC 3 cut(s) 698, 1016, 1052
BciVI GTATCC 1 cut(s) 701
BcoDI GTCTC 8 cut(s) 43, 76, 405, 507, 657, 927, 1056, 1136
BfaI CTAG 2 cut(s) 69, 446
BfmI CTRYAG 1 cut(s) 199
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 1 cut(s) 701
BglII AGATCT 1 cut(s) 102
BisI GCNGC 3 cut(s) 34, 942, 1155
BlsI GCNGC 3 cut(s) 35, 943, 1156
BmcAI AGTACT 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 3 cut(s) 94, 347, 1098
BmrI ACTGGG 1 cut(s) 1101
BmsI GCATC 3 cut(s) 170, 510, 928
BmuI ACTGGG 1 cut(s) 1101
BpiI GAAGAC 2 cut(s) 311, 323
Bpu14I TTCGAA 1 cut(s) 947
BpuEI CTTGAG 2 cut(s) 104, 438
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 341, 909
BsaXI ACNNNNNCTCC 5 cut(s) 30, 629, 659, 1053, 1083
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse1I ACTGG 3 cut(s) 28, 109, 1096
Bse3DI GCAATG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 341, 909
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 4 cut(s) 250, 275, 552, 1149
BseNI ACTGG 3 cut(s) 28, 109, 1096
BseRI GAGGAG 1 cut(s) 526
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 2 cut(s) 928, 1166
BsgI GTGCAG 1 cut(s) 948
BshFI GGCC 3 cut(s) 5, 908, 914
BshNI GGYRCC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 8 cut(s) 43, 76, 405, 507, 657, 927, 1056, 1136
BsmBI CGTCTC 2 cut(s) 43, 927
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 3 cut(s) 5, 908, 914
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 947
Bsp1286I GDGCHC 2 cut(s) 176, 357
Bsp143I GATC 5 cut(s) 102, 163, 448, 588, 1096
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 3 cut(s) 34, 333, 434
BspANI GGCC 3 cut(s) 5, 908, 914
BspCNI CTCAG 4 cut(s) 249, 276, 551, 1150
BspLI GGNNCC 3 cut(s) 94, 347, 1098
BspMAI CTGCAG 1 cut(s) 203
BspPI GGATC 3 cut(s) 443, 1091, 1104
BspQI GCTCTTC 1 cut(s) 591
BspT104I TTCGAA 1 cut(s) 947
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 3 cut(s) 28, 109, 1096
BssECI CCNNGG 2 cut(s) 341, 909
BssMI GATC 5 cut(s) 102, 163, 448, 588, 1096
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 341, 909
Bst4CI ACNGT 2 cut(s) 399, 430
Bst6I CTCTTC 3 cut(s) 510, 591, 1177
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 207
BstBI TTCGAA 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 331, 436
BstDEI CTNAG 7 cut(s) 236, 284, 386, 538, 665, 737, 1158
BstDSI CCRYGG 1 cut(s) 909
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 105, 166, 451, 591, 1099
BstMAI GTCTC 8 cut(s) 43, 76, 405, 507, 657, 927, 1056, 1136
BstMBI GATC 5 cut(s) 102, 163, 448, 588, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 114, 180, 207, 352, 440
BstNSI RCATGY 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 928, 1166
BstV2I GAAGAC 2 cut(s) 311, 323
BstX2I RGATCY 3 cut(s) 102, 448, 1096
BstYI RGATCY 3 cut(s) 102, 448, 1096
BsuI GTATCC 1 cut(s) 701
BsuRI GGCC 3 cut(s) 5, 908, 914
BtgI CCRYGG 1 cut(s) 909
BtrI CACGTC 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 4 cut(s) 35, 208, 782, 1017
Cac8I GCNNGC 2 cut(s) 331, 436
CaiI CAGNNNCTG 2 cut(s) 183, 497
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 42
CseI GACGC 1 cut(s) 512
Csp6I GTAC 2 cut(s) 130, 383
CviAII CATG 8 cut(s) 46, 63, 89, 114, 467, 618, 910, 1028
CviQI GTAC 2 cut(s) 130, 383
DdeI CTNAG 7 cut(s) 236, 284, 386, 538, 665, 737, 1158
DinI GGCGCC 1 cut(s) 94
DpnI GATC 5 cut(s) 104, 165, 450, 590, 1098
DpnII GATC 5 cut(s) 102, 163, 448, 588, 1096
EaeI YGGCCR 3 cut(s) 3, 906, 912
Eam1104I CTCTTC 3 cut(s) 510, 591, 1177
EarI CTCTTC 3 cut(s) 510, 591, 1177
Eco130I CCWWGG 2 cut(s) 341, 909
Eco24I GRGCYC 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 141, 252
Eco88I CYCGRG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 341, 909
EcoT38I GRGCYC 1 cut(s) 357
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 341, 909
Esp3I CGTCTC 2 cut(s) 43, 927
FaeI CATG 8 cut(s) 49, 66, 92, 117, 470, 621, 913, 1031
FatI CATG 8 cut(s) 45, 62, 88, 113, 466, 617, 909, 1027
FauI CCCGC 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 34, 942, 1155
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 3 cut(s) 34, 942, 1155
FspBI CTAG 2 cut(s) 69, 446
GlaI GCGC 1 cut(s) 94
GluI GCNGC 3 cut(s) 34, 942, 1155
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 5, 908, 914
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 49, 66, 92, 117, 470, 621, 913, 1031
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 5 cut(s) 500, 713, 741, 963, 986
HpaII CCGG 1 cut(s) 6
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 132
Hpy188III TCNNGA 5 cut(s) 121, 455, 527, 770, 1079
Hpy8I GTNNAC 1 cut(s) 132
HpyAV CCTTC 2 cut(s) 5, 276
HpyCH4III ACNGT 2 cut(s) 399, 430
HpyCH4IV ACGT 2 cut(s) 52, 320
HpyCH4V TGCA 7 cut(s) 66, 195, 201, 443, 721, 860, 929
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 180, 207, 352, 440
HpyF3I CTNAG 7 cut(s) 236, 284, 386, 538, 665, 737, 1158
HpySE526I ACGT 2 cut(s) 52, 320
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 49, 66, 92, 117, 470, 621, 913, 1031
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Kzo9I GATC 5 cut(s) 102, 163, 448, 588, 1096
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 2 cut(s) 351, 1136
Lsp1109I GCAGC 2 cut(s) 928, 1166
LweI GCATC 3 cut(s) 170, 510, 928
MaeI CTAG 2 cut(s) 69, 446
MaeII ACGT 2 cut(s) 52, 320
MaeIII GTNAC 3 cut(s) 23, 745, 878
MalI GATC 5 cut(s) 104, 165, 450, 590, 1098
MboI GATC 5 cut(s) 102, 163, 448, 588, 1096
MboII GAAGA 8 cut(s) 313, 316, 328, 497, 598, 608, 1101, 1164
MflI RGATCY 3 cut(s) 102, 448, 1096
MhlI GDGCHC 2 cut(s) 176, 357
MlsI TGGCCA 2 cut(s) 908, 914
MluCI AATT 9 cut(s) 190, 277, 476, 613, 656, 790, 816, 1040, 1162
MluNI TGGCCA 2 cut(s) 908, 914
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 2 cut(s) 707, 750
MmeI TCCRAC 2 cut(s) 442, 685
Mox20I TGGCCA 2 cut(s) 908, 914
MroXI GAANNNNTTC 2 cut(s) 504, 791
MscI TGGCCA 2 cut(s) 908, 914
MseI TTAA 3 cut(s) 323, 422, 893
Msp20I TGGCCA 2 cut(s) 908, 914
MspA1I CMGCKG 1 cut(s) 1157
MspI CCGG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 601
MwoI GCNNNNNNNGC 5 cut(s) 114, 180, 207, 352, 440
NarI GGCGCC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 909
NdeII GATC 5 cut(s) 102, 163, 448, 588, 1096
NlaIII CATG 8 cut(s) 49, 66, 92, 117, 470, 621, 913, 1031
NlaIV GGNNCC 3 cut(s) 94, 347, 1098
NmuCI GTSAC 2 cut(s) 23, 878
NspI RCATGY 1 cut(s) 470
NspV TTCGAA 1 cut(s) 947
PciSI GCTCTTC 1 cut(s) 591
PctI GAATGC 1 cut(s) 601
PdmI GAANNNNTTC 2 cut(s) 504, 791
PfeI GAWTC 3 cut(s) 500, 963, 986
PkrI GCNGC 3 cut(s) 35, 943, 1156
PleI GAGTC 2 cut(s) 707, 749
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 2 cut(s) 707, 749
PspN4I GGNNCC 3 cut(s) 94, 347, 1098
PspPI GGNCC 1 cut(s) 42
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PstI CTGCAG 1 cut(s) 203
PstNI CAGNNNCTG 2 cut(s) 183, 497
PsuI RGATCY 3 cut(s) 102, 448, 1096
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 2 cut(s) 131, 384
RsaNI GTAC 2 cut(s) 130, 383
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 3 cut(s) 323, 422, 893
SatI GCNGC 3 cut(s) 34, 942, 1155
Sau3AI GATC 5 cut(s) 102, 163, 448, 588, 1096
Sau96I GGNCC 1 cut(s) 42
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 2 cut(s) 707, 750
SduI GDGCHC 2 cut(s) 176, 357
SfaNI GCATC 3 cut(s) 170, 510, 928
SfcI CTRYAG 1 cut(s) 199
SfoI GGCGCC 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 947
SinI GGWCC 1 cut(s) 42
SmlI CTYRAG 2 cut(s) 119, 453
SmoI CTYRAG 2 cut(s) 119, 453
Sse9I AATT 9 cut(s) 190, 277, 476, 613, 656, 790, 816, 1040, 1162
SsiI CCGC 3 cut(s) 34, 333, 434
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 2 cut(s) 69, 446
StyI CCWWGG 2 cut(s) 341, 909
TaaI ACNGT 2 cut(s) 399, 430
TaiI ACGT 2 cut(s) 55, 323
TaqI TCGA 2 cut(s) 947, 1014
TasI AATT 9 cut(s) 190, 277, 476, 613, 656, 790, 816, 1040, 1162
TatI WGTACW 1 cut(s) 382
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 3 cut(s) 500, 963, 986
Tru1I TTAA 3 cut(s) 323, 422, 893
Tru9I TTAA 3 cut(s) 323, 422, 893
TscAI CASTG 4 cut(s) 35, 208, 789, 1024
TseFI GTSAC 2 cut(s) 23, 878
TseI GCWGC 2 cut(s) 941, 1154
Tsp45I GTSAC 2 cut(s) 23, 878
TspDTI ATGAA 3 cut(s) 606, 784, 1131
TspRI CASTG 4 cut(s) 35, 208, 789, 1024
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 2 cut(s) 613, 790
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 2 cut(s) 504, 791
XspI CTAG 2 cut(s) 69, 446
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.