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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 327 |
| AccII | CGCG | 2 cut(s) | 79, 125 |
| AciI | CCGC | 6 cut(s) | 44, 77, 125, 212, 229, 256 |
| AcsI | RAATTY | 3 cut(s) | 206, 234, 337 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 156, 327 |
| Alw26I | GTCTC | 2 cut(s) | 9, 91 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 327 |
| ApeKI | GCWGC | 1 cut(s) | 223 |
| ApoI | RAATTY | 3 cut(s) | 206, 234, 337 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 155, 187 |
| AspLEI | GCGC | 2 cut(s) | 81, 228 |
| BbvI | GCAGC | 1 cut(s) | 235 |
| BcoDI | GTCTC | 2 cut(s) | 9, 91 |
| BfaI | CTAG | 2 cut(s) | 161, 263 |
| BfoI | RGCGCY | 1 cut(s) | 229 |
| BisI | GCNGC | 3 cut(s) | 77, 224, 229 |
| BlpI | GCTNAGC | 1 cut(s) | 361 |
| BlsI | GCNGC | 3 cut(s) | 78, 225, 230 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 361 |
| BpuEI | CTTGAG | 1 cut(s) | 200 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 16, 46 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 156, 327 |
| Bse3DI | GCAATG | 1 cut(s) | 71 |
| BseGI | GGATG | 1 cut(s) | 121 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 156, 327 |
| BseMI | GCAATG | 1 cut(s) | 71 |
| BseMII | CTCAG | 2 cut(s) | 179, 295 |
| BseRI | GAGGAG | 2 cut(s) | 79, 205 |
| BseXI | GCAGC | 1 cut(s) | 235 |
| BseYI | CCCAGC | 1 cut(s) | 327 |
| Bsh1236I | CGCG | 2 cut(s) | 79, 125 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 156, 327 |
| BsmAI | GTCTC | 2 cut(s) | 9, 91 |
| BsmI | GAATGC | 1 cut(s) | 297 |
| Bsp143I | GATC | 2 cut(s) | 13, 130 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 361 |
| BspACI | CCGC | 6 cut(s) | 44, 77, 125, 212, 229, 256 |
| BspCNI | CTCAG | 2 cut(s) | 180, 294 |
| BspFNI | CGCG | 2 cut(s) | 79, 125 |
| BsrDI | GCAATG | 1 cut(s) | 71 |
| BssMI | GATC | 2 cut(s) | 13, 130 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 311 |
| BstDEI | CTNAG | 3 cut(s) | 188, 281, 361 |
| BstF5I | GGATG | 1 cut(s) | 121 |
| BstFNI | CGCG | 2 cut(s) | 79, 125 |
| BstH2I | RGCGCY | 1 cut(s) | 229 |
| BstHHI | GCGC | 2 cut(s) | 81, 228 |
| BstKTI | GATC | 2 cut(s) | 16, 133 |
| BstMAI | GTCTC | 2 cut(s) | 9, 91 |
| BstMBI | GATC | 2 cut(s) | 13, 130 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 237, 311, 357 |
| BstUI | CGCG | 2 cut(s) | 79, 125 |
| BstV1I | GCAGC | 1 cut(s) | 235 |
| BtgZI | GCGATG | 2 cut(s) | 39, 106 |
| BtsCI | GGATG | 1 cut(s) | 121 |
| CaiI | CAGNNNCTG | 1 cut(s) | 327 |
| CfoI | GCGC | 2 cut(s) | 81, 228 |
| CviAII | CATG | 1 cut(s) | 306 |
| CviJI | RGCY | 6 cut(s) | 23, 160, 185, 272, 327, 360 |
| CviKI_1 | RGCY | 6 cut(s) | 23, 160, 185, 272, 327, 360 |
| DdeI | CTNAG | 3 cut(s) | 188, 281, 361 |
| DpnI | GATC | 2 cut(s) | 15, 132 |
| DpnII | GATC | 2 cut(s) | 13, 130 |
| FaeI | CATG | 1 cut(s) | 309 |
| FaiI | YATR | 6 cut(s) | 254, 307, 345, 353, 355, 357 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 325, 357 |
| FatI | CATG | 1 cut(s) | 305 |
| FauI | CCCGC | 2 cut(s) | 51, 249 |
| Fnu4HI | GCNGC | 3 cut(s) | 77, 224, 229 |
| FokI | GGATG | 1 cut(s) | 128 |
| Fsp4HI | GCNGC | 3 cut(s) | 77, 224, 229 |
| FspBI | CTAG | 2 cut(s) | 161, 263 |
| GlaI | GCGC | 2 cut(s) | 80, 227 |
| GluI | GCNGC | 3 cut(s) | 77, 224, 229 |
| GsaI | CCCAGC | 1 cut(s) | 331 |
| HaeII | RGCGCY | 1 cut(s) | 229 |
| HhaI | GCGC | 2 cut(s) | 81, 228 |
| Hin1II | CATG | 1 cut(s) | 309 |
| Hin6I | GCGC | 2 cut(s) | 79, 226 |
| HinP1I | GCGC | 2 cut(s) | 79, 226 |
| HinfI | GANTC | 3 cut(s) | 84, 164, 378 |
| Hpy188I | TCNGA | 2 cut(s) | 169, 189 |
| HpyCH4IV | ACGT | 1 cut(s) | 242 |
| HpyCH4V | TGCA | 3 cut(s) | 223, 295, 351 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 237, 311, 357 |
| HpyF3I | CTNAG | 3 cut(s) | 188, 281, 361 |
| HpySE526I | ACGT | 1 cut(s) | 242 |
| Hsp92II | CATG | 1 cut(s) | 309 |
| HspAI | GCGC | 2 cut(s) | 79, 226 |
| Kzo9I | GATC | 2 cut(s) | 13, 130 |
| LpnPI | CCDG | 3 cut(s) | 268, 313, 334 |
| Lsp1109I | GCAGC | 1 cut(s) | 235 |
| MaeI | CTAG | 2 cut(s) | 161, 263 |
| MaeII | ACGT | 1 cut(s) | 242 |
| MalI | GATC | 2 cut(s) | 15, 132 |
| MboI | GATC | 2 cut(s) | 13, 130 |
| MfeI | CAATTG | 1 cut(s) | 17 |
| MluCI | AATT | 4 cut(s) | 17, 206, 234, 337 |
| MlyI | GAGTC | 2 cut(s) | 93, 158 |
| MnlI | CCTC | 6 cut(s) | 42, 61, 100, 175, 183, 317 |
| MseI | TTAA | 1 cut(s) | 245 |
| MslI | CAYNNNNRTG | 1 cut(s) | 245 |
| MunI | CAATTG | 1 cut(s) | 17 |
| Mva1269I | GAATGC | 1 cut(s) | 297 |
| MvnI | CGCG | 2 cut(s) | 79, 125 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 237, 311, 357 |
| NdeII | GATC | 2 cut(s) | 13, 130 |
| NlaIII | CATG | 1 cut(s) | 309 |
| NmeAIII | GCCGAG | 1 cut(s) | 107 |
| PctI | GAATGC | 1 cut(s) | 297 |
| PfeI | GAWTC | 1 cut(s) | 378 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 327 |
| PkrI | GCNGC | 3 cut(s) | 78, 225, 230 |
| PleI | GAGTC | 2 cut(s) | 92, 158 |
| PpsI | GAGTC | 2 cut(s) | 92, 158 |
| PspFI | CCCAGC | 1 cut(s) | 327 |
| PstNI | CAGNNNCTG | 1 cut(s) | 327 |
| RseI | CAYNNNNRTG | 1 cut(s) | 245 |
| SaqAI | TTAA | 1 cut(s) | 245 |
| SatI | GCNGC | 3 cut(s) | 77, 224, 229 |
| Sau3AI | GATC | 2 cut(s) | 13, 130 |
| SchI | GAGTC | 2 cut(s) | 93, 158 |
| SetI | ASST | 3 cut(s) | 34, 245, 287 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 245 |
| SmlI | CTYRAG | 1 cut(s) | 179 |
| SmoI | CTYRAG | 1 cut(s) | 179 |
| Sse9I | AATT | 4 cut(s) | 17, 206, 234, 337 |
| SsiI | CCGC | 6 cut(s) | 44, 77, 125, 212, 229, 256 |
| SspMI | CTAG | 2 cut(s) | 161, 263 |
| TaiI | ACGT | 1 cut(s) | 245 |
| TasI | AATT | 4 cut(s) | 17, 206, 234, 337 |
| TauI | GCSGC | 2 cut(s) | 79, 231 |
| TfiI | GAWTC | 1 cut(s) | 378 |
| Tru1I | TTAA | 1 cut(s) | 245 |
| Tru9I | TTAA | 1 cut(s) | 245 |
| TseI | GCWGC | 1 cut(s) | 223 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 327 |
| XapI | RAATTY | 3 cut(s) | 206, 234, 337 |
| XspI | CTAG | 2 cut(s) | 161, 263 |