FvH4_7g26490

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000588
Physical Location & Seq
Forward (+)
19991850 .. 19998355
6506 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26490.t1

Sequence Viewer

Length: 1077 bp
ATGTTAATGCTGGTAGACTGCTCCAACTGCCACACACCTCTGCAGCTGCCGCCTGGCGCTCAGATCATCCGCTGCGGCTTATGCGACGCCGTCACGCGTATTGCCGGAGCCTCCCGATCACTCACTTCTCCTCCGCCGCGCCAACCCCCATCATCCTCCTCTTCCCACGCGCCTTCGCCGTCCAGCCACGCGCCGCCGCCGCCGTCGTCGGCACACGGACGGAAGCGGGCGGTGGTGTGCGCCGTGTCGTACAAGCGGTCGCGGCACGAGCTCAAGGGGTGCATTAACGACGCCAAGTGTATGAAGTATTTGCTGGTCAACAAGTTCAAGTTTCCTGAGTCTTCCGTTCTCATGCTCACAGAAGAAGAAGCTGATCCTTTTCGAAGGCCGACCAAGCAAAACATGAGAATGGCTCTGTTCTGGCTTGTGCAAGGTTGTCAAGCAGGGGATTCTTTGGTGTTTCATTATTCGGGGCATGGATCACAGCAGCGGAACTACACTGGAGACGAGGTGGATGGATATGATGAGACACTGTGTCCCTCGGATTTTGAGACTCAGGGGATGATCGTGGATGATGAGATCAATGCAACAATTGTTAACCCTCTTCCTGCTGGAGCTAAACTTCATGCTATTGTAGATGCTTGCCATAGTGGTACTGTGCTAGATTTGCCATTTCTCTGTAGAATGGACAGAAATGGGAGGTACATATGGGAGGACCATCGTCCTCGATCAGGTGTTTGGAAGGGAACAAGTGGTGGTGAGGCCATTTCCTTCAGTGGCTGCGACGATGATCAGACCTCTGCTGATACCTCAGCTCTGTCAAAGATTACTTCAACTGGTGCCATGACTTATGCTTTCATCCAAGCCATAGAGCGTGGACATGCAACTACATACGGGAACATGATGAATGCAATGCGATCCACCATCCGTAACACCGATGGTGGTGCTGGAGGCGGTATTGTGACATCCCTTATCTCCATGCTTTTGACAGGAGGGAGTCTCGGAAAGCTGAGACAGGAACCACAGCTATCTGCCAATCAACAATTTGATGTGTATACAAAACCTTTCTCCCTATAG

Protein Analysis

359

Amino Acids

38.75

Weight (kDa)

7.13

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 7 - 31 1.8e-08 LSD1 zinc finger
Peptidase_C14 PF00656 75 - 347 2e-36 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 839
AccI GTMKAC 2 cut(s) 15, 1055
AccII CGCG 5 cut(s) 97, 139, 170, 191, 262
AclWI GGATC 3 cut(s) 368, 487, 912
AcuI CTGAAG 1 cut(s) 757
AcyI GRCGYC 2 cut(s) 87, 291
AfaI GTAC 3 cut(s) 251, 655, 704
AfiI CCNNNNNNNGG 1 cut(s) 731
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 2 cut(s) 328, 834
AhdI GACNNNNNGTC 1 cut(s) 534
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 7 cut(s) 46, 271, 371, 617, 815, 1009, 1027
AluI AGCT 7 cut(s) 46, 271, 371, 617, 815, 1009, 1027
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 5 cut(s) 498, 521, 545, 1004, 1006
AlwI GGATC 3 cut(s) 368, 487, 912
AlwNI CAGNNNCTG 1 cut(s) 780
AoxI GGCC 2 cut(s) 386, 762
ApeKI GCWGC 5 cut(s) 43, 46, 72, 487, 780
AspLEI GCGC 5 cut(s) 59, 141, 172, 193, 242
AspS9I GGNCC 1 cut(s) 715
AsuHPI GGTGA 1 cut(s) 770
AsuII TTCGAA 1 cut(s) 382
AvaII GGWCC 1 cut(s) 715
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 1 cut(s) 273
BauI CACGAG 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 811
BbvI GCAGC 5 cut(s) 33, 55, 59, 499, 767
BccI CCATC 5 cut(s) 157, 509, 726, 932, 932
BceAI ACGGC 4 cut(s) 74, 163, 187, 227
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 1 cut(s) 54
BclI TGATCA 1 cut(s) 790
BcoDI GTCTC 5 cut(s) 498, 521, 545, 1004, 1006
BfaI CTAG 1 cut(s) 662
BfmI CTRYAG 3 cut(s) 41, 679, 1073
BfoI RGCGCY 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 715
BmeRI GACNNNNNGTC 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 3 cut(s) 109, 841, 1020
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 628
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 4 cut(s) 397, 429, 984, 1016
BpmI CTGGAG 3 cut(s) 522, 633, 969
Bpu10I CCTNAGC 1 cut(s) 811
Bpu14I TTCGAA 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 257
BsaHI GRCGYC 2 cut(s) 87, 291
BsaJI CCNNGG 1 cut(s) 540
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse1I ACTGG 2 cut(s) 505, 841
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 8 cut(s) 66, 152, 520, 567, 577, 858, 924, 965
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 5 cut(s) 74, 327, 569, 825, 1001
BseNI ACTGG 2 cut(s) 505, 841
BseRI GAGGAG 2 cut(s) 120, 148
BseXI GCAGC 5 cut(s) 33, 55, 59, 499, 767
Bsh1236I CGCG 5 cut(s) 97, 139, 170, 191, 262
Bsh1285I CGRYCG 1 cut(s) 260
BshFI GGCC 2 cut(s) 388, 764
BshNI GGYRCC 1 cut(s) 839
BsiEI CGRYCG 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 731
BsmAI GTCTC 5 cut(s) 498, 521, 545, 1004, 1006
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 522
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 2 cut(s) 388, 764
Bsp119I TTCGAA 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
BspANI GGCC 2 cut(s) 388, 764
BspCNI CTCAG 5 cut(s) 73, 328, 568, 824, 1002
BspFNI CGCG 5 cut(s) 97, 139, 170, 191, 262
BspLI GGNNCC 3 cut(s) 109, 841, 1020
BspMAI CTGCAG 1 cut(s) 45
BspPI GGATC 3 cut(s) 368, 487, 912
BspT104I TTCGAA 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 839
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 2 cut(s) 505, 841
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
BssNAI GTATAC 1 cut(s) 1056
BssNI GRCGYC 2 cut(s) 87, 291
BssSI CACGAG 1 cut(s) 266
Bst1107I GTATAC 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 266
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 534, 658
Bst6I CTCTTC 2 cut(s) 166, 609
BstACI GRCGYC 2 cut(s) 87, 291
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 228, 643
BstDEI CTNAG 5 cut(s) 60, 336, 555, 811, 1010
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 8 cut(s) 66, 152, 520, 567, 577, 858, 924, 965
BstFNI CGCG 5 cut(s) 97, 139, 170, 191, 262
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 5 cut(s) 59, 141, 172, 193, 242
BstKTI GATC 9 cut(s) 66, 119, 376, 482, 567, 582, 731, 793, 920
BstMAI GTCTC 5 cut(s) 498, 521, 545, 1004, 1006
BstMBI GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
BstMCI CGRYCG 1 cut(s) 260
BstMWI GCNNNNNNNGC 8 cut(s) 27, 49, 81, 199, 262, 268, 394, 667
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 884
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 3 cut(s) 41, 679, 1073
BstUI CGCG 5 cut(s) 97, 139, 170, 191, 262
BstV1I GCAGC 5 cut(s) 33, 55, 59, 499, 767
BstV2I GAAGAC 1 cut(s) 333
BstZ17I GTATAC 1 cut(s) 1056
BsuRI GGCC 2 cut(s) 388, 764
BtsCI GGATG 8 cut(s) 66, 152, 520, 567, 577, 858, 924, 965
BtsIMutI CAGTG 3 cut(s) 498, 530, 781
Cac8I GCNNGC 2 cut(s) 228, 643
CaiI CAGNNNCTG 1 cut(s) 780
CfoI GCGC 5 cut(s) 59, 141, 172, 193, 242
Cfr13I GGNCC 1 cut(s) 715
CseI GACGC 2 cut(s) 95, 299
Csp6I GTAC 3 cut(s) 250, 654, 703
CviAII CATG 8 cut(s) 352, 403, 476, 626, 844, 881, 901, 979
CviQI GTAC 3 cut(s) 250, 654, 703
DdeI CTNAG 5 cut(s) 60, 336, 555, 811, 1010
DpnI GATC 9 cut(s) 65, 118, 375, 481, 566, 581, 730, 792, 919
DpnII GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
DriI GACNNNNNGTC 1 cut(s) 534
Eam1104I CTCTTC 2 cut(s) 166, 609
Eam1105I GACNNNNNGTC 1 cut(s) 534
EarI CTCTTC 2 cut(s) 166, 609
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 715
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 757
EcoICRI GAGCTC 1 cut(s) 271
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 8 cut(s) 355, 406, 479, 629, 847, 884, 904, 982
FaqI GGGAC 1 cut(s) 522
FatI CATG 8 cut(s) 351, 402, 475, 625, 843, 880, 900, 978
FauI CCCGC 1 cut(s) 219
FauNDI CATATG 1 cut(s) 707
FbaI TGATCA 1 cut(s) 790
FblI GTMKAC 2 cut(s) 15, 1055
FokI GGATG 8 cut(s) 53, 139, 527, 574, 584, 845, 911, 952
FriOI GRGCYC 1 cut(s) 273
FspBI CTAG 1 cut(s) 662
GlaI GCGC 5 cut(s) 58, 140, 171, 192, 241
GsuI CTGGAG 3 cut(s) 522, 633, 969
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 2 cut(s) 388, 764
HapII CCGG 1 cut(s) 105
HgaI GACGC 2 cut(s) 95, 299
HhaI GCGC 5 cut(s) 59, 141, 172, 193, 242
Hin1I GRCGYC 2 cut(s) 87, 291
Hin1II CATG 8 cut(s) 355, 406, 479, 629, 847, 884, 904, 982
Hin6I GCGC 5 cut(s) 57, 139, 170, 191, 240
HinP1I GCGC 5 cut(s) 57, 139, 170, 191, 240
HincII GTYRAC 2 cut(s) 319, 598
HindII GTYRAC 2 cut(s) 319, 598
HinfI GANTC 4 cut(s) 338, 449, 553, 997
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 770
Hpy166II GTNNAC 5 cut(s) 16, 319, 598, 878, 1056
Hpy188I TCNGA 4 cut(s) 63, 544, 795, 1004
Hpy188III TCNNGA 2 cut(s) 114, 335
Hpy8I GTNNAC 5 cut(s) 16, 319, 598, 878, 1056
Hpy99I CGWCG 5 cut(s) 89, 208, 211, 293, 788
HpyAV CCTTC 4 cut(s) 183, 378, 736, 781
HpyCH4III ACNGT 2 cut(s) 534, 658
HpyCH4V TGCA 6 cut(s) 43, 282, 430, 587, 884, 911
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 49, 81, 199, 262, 268, 394, 667
HpyF3I CTNAG 5 cut(s) 60, 336, 555, 811, 1010
Hsp92I GRCGYC 2 cut(s) 87, 291
Hsp92II CATG 8 cut(s) 355, 406, 479, 629, 847, 884, 904, 982
HspAI GCGC 5 cut(s) 57, 139, 170, 191, 240
Ksp22I TGATCA 1 cut(s) 790
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
LmnI GCTCC 3 cut(s) 26, 107, 614
Lsp1109I GCAGC 5 cut(s) 33, 55, 59, 499, 767
LweI GCATC 1 cut(s) 628
MaeI CTAG 1 cut(s) 662
MaeIII GTNAC 3 cut(s) 91, 929, 961
MalI GATC 9 cut(s) 65, 118, 375, 481, 566, 581, 730, 792, 919
MboI GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
MboII GAAGA 5 cut(s) 153, 333, 374, 377, 596
MfeI CAATTG 1 cut(s) 591
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 591, 1043
MluI ACGCGT 1 cut(s) 95
MlyI GAGTC 3 cut(s) 347, 547, 1006
MmeI TCCRAC 1 cut(s) 48
MseI TTAA 3 cut(s) 5, 285, 597
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 3 cut(s) 46, 72, 490
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 1 cut(s) 54
MunI CAATTG 1 cut(s) 591
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 5 cut(s) 97, 139, 170, 191, 262
MwoI GCNNNNNNNGC 8 cut(s) 27, 49, 81, 199, 262, 268, 394, 667
NdeI CATATG 1 cut(s) 707
NdeII GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
NlaIII CATG 8 cut(s) 355, 406, 479, 629, 847, 884, 904, 982
NlaIV GGNNCC 3 cut(s) 109, 841, 1020
NmuCI GTSAC 2 cut(s) 91, 961
NspI RCATGY 1 cut(s) 884
NspV TTCGAA 1 cut(s) 382
PctI GAATGC 1 cut(s) 913
PfeI GAWTC 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 89
PleI GAGTC 3 cut(s) 346, 547, 1005
PpsI GAGTC 3 cut(s) 346, 547, 1005
PshAI GACNNNNGTC 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 109, 841, 1020
PspPI GGNCC 1 cut(s) 715
PstI CTGCAG 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 780
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 3 cut(s) 251, 655, 704
RsaNI GTAC 3 cut(s) 250, 654, 703
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 3 cut(s) 5, 285, 597
Sau3AI GATC 9 cut(s) 63, 116, 373, 479, 564, 579, 728, 790, 917
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 3 cut(s) 347, 547, 1006
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 628
SfcI CTRYAG 3 cut(s) 41, 679, 1073
SfuI TTCGAA 1 cut(s) 382
SinI GGWCC 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 591, 1043
SspMI CTAG 1 cut(s) 662
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 534, 658
TaqI TCGA 2 cut(s) 382, 727
TasI AATT 2 cut(s) 591, 1043
TauI GCSGC 7 cut(s) 52, 78, 139, 196, 199, 202, 265
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 5, 285, 597
Tru9I TTAA 3 cut(s) 5, 285, 597
TscAI CASTG 3 cut(s) 505, 537, 781
TseFI GTSAC 2 cut(s) 91, 961
TseI GCWGC 5 cut(s) 43, 46, 72, 487, 780
Tsp45I GTSAC 2 cut(s) 91, 961
TspDTI ATGAA 5 cut(s) 317, 452, 614, 847, 920
TspGWI ACGGA 4 cut(s) 231, 235, 334, 917
TspRI CASTG 3 cut(s) 505, 537, 781
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 715
XagI CCTNNNNNAGG 1 cut(s) 729
XceI RCATGY 1 cut(s) 884
XmiI GTMKAC 2 cut(s) 15, 1055
XspI CTAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.