Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0000588

Physical Location & Seq

Genomic Coordinates Forward (+)

60123003 .. 60125488

Gene Structure 2486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG379400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGTTAATGCTGGTAGACTGCTCCAACTGCCTCACACCTCTCCAGCTGCCAGCTGGCGCTCAGGTAATCCGCTGCGGCTTATGCAACGCCGTCACGCGCATCGCCGGAACCTCCTCCTCCCGATCTCAACTTCCTCCTCATCCGCAATCCCCCCACGCGCCGCCGTCGCCGTACAGGCACGCGCCGCGGGGGGCGCCGCCGTCGGCCCACGGACGGAAGAGGGCGGTGCTATGCGCCGTGTCGTACAAGGGAACGCGACACGAGCTCAAGGGCTGCATTAACGACGCCAAGTGTATGAAGTATTTGCTGGTCAACAAGTTCAAGTTTCCGGAGTCTTCCATTGTCATGCTCACAGAAGAAGAAGCTGATCCTTTTCGATGGCCAACCAAACAAAACATGAGAATGGCGCTGTTTTGGCTTGTGCAAGGTTGTCAAGCAGGGGATTCTTTGGTGTTTCATTATTCGGGGCATGGATCACAGCAGCGGAACTACACTGGAGACGAGGTGGATGGATATGATGAGACACTGTGCCCCACGGATTATGAGACTCAGGGGATGATCGTGGATGATGAGATCAATGCAACAATTGTTAGACCTCTTCCTGCTGGAGTTAAACTTCATGCTATTGTAGATGCTTGCCATAGTGGTACTGTGCTAGATTTGCCATTTCTCTGTAGAATGGACAGAAATGGGAGGTACATATGGGAGGACCATCGTCCTCGATCAGGTGTTTGGAAGGGAACAAGTGGTGGTGAGGCCATTTCCTTCAGTGGCTGTGACGATGATCAGACCTCTGCTGATACCTCAGTAAGTAGTGGACAAAAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0010941 GO:0010942 GO:0016787 GO:0019538 GO:0043067 GO:0043068 GO:0043170 GO:0044238 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0065007 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C14 zf-LSD1

Protein Analysis

276

Amino Acids

30.27

Weight (kDa)

6.78

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-LSD1	PF06943	7 - 31	4.3e-07	LSD1 zinc finger
Peptidase_C14	PF00656	73 - 271	8.8e-30	Caspase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02170
fragaria_vesca	FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica	MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica	Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis	pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis	RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata	RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora	Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii	Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa	Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha	Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana	Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	193
AccI	GTMKAC	1 cut(s)	15
AccII	CGCG	5 cut(s)	97, 158, 182, 187, 256
AccIII	TCCGGA	1 cut(s)	328
AciI	CCGC	9 cut(s)	70, 75, 143, 161, 185, 187, 197, 224, 484
AclWI	GGATC	2 cut(s)	362, 481
AcoI	YGGCCR	1 cut(s)	380
AcuI	CTGAAG	1 cut(s)	751
AcyI	GRCGYC	2 cut(s)	194, 285
AfaI	GTAC	4 cut(s)	173, 245, 649, 698
AfiI	CCNNNNNNNGG	2 cut(s)	213, 725
AgsI	TTSAA	1 cut(s)	322
AluBI	AGCT	5 cut(s)	46, 53, 265, 365, 826
AluI	AGCT	5 cut(s)	46, 53, 265, 365, 826
Alw21I	GWGCWC	1 cut(s)	267
Alw26I	GTCTC	3 cut(s)	492, 515, 539
AlwI	GGATC	2 cut(s)	362, 481
AlwNI	CAGNNNCTG	1 cut(s)	774
Aor13HI	TCCGGA	1 cut(s)	328
AoxI	GGCC	3 cut(s)	204, 380, 756
ApeKI	GCWGC	4 cut(s)	46, 72, 273, 481
ArsI	GACNNNNNNTTYG	1 cut(s)	810
AspLEI	GCGC	7 cut(s)	59, 99, 160, 184, 196, 236, 409
AspS9I	GGNCC	2 cut(s)	205, 709
AsuHPI	GGTGA	1 cut(s)	764
AvaII	GGWCC	1 cut(s)	709
BaeGI	GKGCMC	1 cut(s)	533
BaeI	ACNNNNGTAYC	2 cut(s)	792, 825
BalI	TGGCCA	1 cut(s)	382
BanI	GGYRCC	1 cut(s)	193
BanII	GRGCYC	1 cut(s)	267
BauI	CACGAG	1 cut(s)	260
BbsI	GAAGAC	1 cut(s)	327
Bbv12I	GWGCWC	1 cut(s)	267
BbvI	GCAGC	4 cut(s)	33, 59, 260, 493
BccI	CCATC	3 cut(s)	372, 503, 720
BceAI	ACGGC	5 cut(s)	74, 148, 154, 184, 221
BclI	TGATCA	1 cut(s)	784
BcoDI	GTCTC	3 cut(s)	492, 515, 539
BfaI	CTAG	1 cut(s)	656
BfmI	CTRYAG	1 cut(s)	673
BfoI	RGCGCY	3 cut(s)	60, 197, 410
BglI	GCCNNNNNGGC	1 cut(s)	175
BisI	GCNGC	8 cut(s)	47, 73, 76, 161, 185, 197, 274, 482
BlsI	GCNGC	8 cut(s)	48, 74, 77, 162, 186, 198, 275, 483
Bme18I	GGWCC	1 cut(s)	709
BmgT120I	GGNCC	2 cut(s)	205, 709
BmiI	GGNNCC	2 cut(s)	109, 195
BmsI	GCATC	2 cut(s)	108, 622
BoxI	GACNNNNGTC	1 cut(s)	714
BpiI	GAAGAC	1 cut(s)	327
BpmI	CTGGAG	3 cut(s)	26, 516, 627
Bpu10I	CCTNAGC	1 cut(s)	60
BpuEI	CTTGAG	1 cut(s)	251
BsaHI	GRCGYC	2 cut(s)	194, 285
BsaJI	CCNNGG	3 cut(s)	185, 208, 534
BsaWI	WCCGGW	1 cut(s)	328
BsaXI	ACNNNNNCTCC	2 cut(s)	489, 519
Bsc4I	CCNNNNNNNGG	2 cut(s)	213, 725
Bse1I	ACTGG	1 cut(s)	499
BseAI	TCCGGA	1 cut(s)	328
BseDI	CCNNGG	3 cut(s)	185, 208, 534
BseGI	GGATG	4 cut(s)	139, 514, 561, 571
BseLI	CCNNNNNNNGG	2 cut(s)	213, 725
BseMII	CTCAG	3 cut(s)	74, 563, 819
BseNI	ACTGG	1 cut(s)	499
BseRI	GAGGAG	3 cut(s)	103, 106, 126
BseSI	GKGCMC	1 cut(s)	533
BseXI	GCAGC	4 cut(s)	33, 59, 260, 493
Bsh1236I	CGCG	5 cut(s)	97, 158, 182, 187, 256
BshFI	GGCC	3 cut(s)	206, 382, 758
BshNI	GGYRCC	1 cut(s)	193
BsiHKAI	GWGCWC	1 cut(s)	267
BsiSI	CCGG	2 cut(s)	105, 329
BslI	CCNNNNNNNGG	2 cut(s)	213, 725
BsmAI	GTCTC	3 cut(s)	492, 515, 539
BsmBI	CGTCTC	1 cut(s)	492
BsnI	GGCC	3 cut(s)	206, 382, 758
Bsp1286I	GDGCHC	2 cut(s)	267, 533
Bsp13I	TCCGGA	1 cut(s)	328
Bsp143I	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
BspACI	CCGC	9 cut(s)	70, 75, 143, 161, 185, 187, 197, 224, 484
BspANI	GGCC	3 cut(s)	206, 382, 758
BspCNI	CTCAG	3 cut(s)	73, 562, 818
BspEI	TCCGGA	1 cut(s)	328
BspFNI	CGCG	5 cut(s)	97, 158, 182, 187, 256
BspLI	GGNNCC	2 cut(s)	109, 195
BspPI	GGATC	2 cut(s)	362, 481
BspT107I	GGYRCC	1 cut(s)	193
BsrI	ACTGG	1 cut(s)	499
BssECI	CCNNGG	3 cut(s)	185, 208, 534
BssMI	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
BssNI	GRCGYC	2 cut(s)	194, 285
BssSI	CACGAG	1 cut(s)	260
Bst2BI	CACGAG	1 cut(s)	260
Bst4CI	ACNGT	2 cut(s)	528, 652
Bst6I	CTCTTC	2 cut(s)	212, 603
BstACI	GRCGYC	2 cut(s)	194, 285
BstC8I	GCNNGC	4 cut(s)	51, 55, 180, 637
BstDEI	CTNAG	3 cut(s)	60, 549, 805
BstDSI	CCRYGG	3 cut(s)	185, 208, 534
BstENI	CCTNNNNNAGG	1 cut(s)	723
BstF5I	GGATG	4 cut(s)	139, 514, 561, 571
BstFNI	CGCG	5 cut(s)	97, 158, 182, 187, 256
BstH2I	RGCGCY	3 cut(s)	60, 197, 410
BstHHI	GCGC	7 cut(s)	59, 99, 160, 184, 196, 236, 409
BstKTI	GATC	7 cut(s)	125, 370, 476, 561, 576, 725, 787
BstMAI	GTCTC	3 cut(s)	492, 515, 539
BstMBI	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
BstMWI	GCNNNNNNNGC	9 cut(s)	27, 81, 166, 175, 184, 193, 262, 415, 661
BstPAI	GACNNNNGTC	1 cut(s)	714
BstSFI	CTRYAG	1 cut(s)	673
BstSLI	GKGCMC	1 cut(s)	533
BstUI	CGCG	5 cut(s)	97, 158, 182, 187, 256
BstV1I	GCAGC	4 cut(s)	33, 59, 260, 493
BstV2I	GAAGAC	1 cut(s)	327
BsuRI	GGCC	3 cut(s)	206, 382, 758
BtgI	CCRYGG	3 cut(s)	185, 208, 534
BtgZI	GCGATG	1 cut(s)	85
BtsCI	GGATG	4 cut(s)	139, 514, 561, 571
BtsIMutI	CAGTG	3 cut(s)	492, 524, 775
Cac8I	GCNNGC	4 cut(s)	51, 55, 180, 637
CaiI	CAGNNNCTG	1 cut(s)	774
CfoI	GCGC	7 cut(s)	59, 99, 160, 184, 196, 236, 409
Cfr13I	GGNCC	2 cut(s)	205, 709
Cfr42I	CCGCGG	1 cut(s)	188
CseI	GACGC	1 cut(s)	293
Csp6I	GTAC	4 cut(s)	172, 244, 648, 697
CviAII	CATG	4 cut(s)	346, 397, 470, 620
CviQI	GTAC	4 cut(s)	172, 244, 648, 697
DdeI	CTNAG	3 cut(s)	60, 549, 805
DinI	GGCGCC	1 cut(s)	195
DpnI	GATC	7 cut(s)	124, 369, 475, 560, 575, 724, 786
DpnII	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
EaeI	YGGCCR	1 cut(s)	380
Eam1104I	CTCTTC	2 cut(s)	212, 603
EarI	CTCTTC	2 cut(s)	212, 603
Ecl136II	GAGCTC	1 cut(s)	265
Eco24I	GRGCYC	1 cut(s)	267
Eco47I	GGWCC	1 cut(s)	709
Eco53kI	GAGCTC	1 cut(s)	265
Eco57I	CTGAAG	1 cut(s)	751
EcoICRI	GAGCTC	1 cut(s)	265
EcoNI	CCTNNNNNAGG	1 cut(s)	723
EcoT38I	GRGCYC	1 cut(s)	267
EgeI	GGCGCC	1 cut(s)	195
EheI	GGCGCC	1 cut(s)	195
Esp3I	CGTCTC	1 cut(s)	492
FaeI	CATG	4 cut(s)	349, 400, 473, 623
FatI	CATG	4 cut(s)	345, 396, 469, 619
FauI	CCCGC	1 cut(s)	180
FauNDI	CATATG	1 cut(s)	701
FbaI	TGATCA	1 cut(s)	784
FblI	GTMKAC	1 cut(s)	15
Fnu4HI	GCNGC	8 cut(s)	47, 73, 76, 161, 185, 197, 274, 482
FokI	GGATG	4 cut(s)	126, 521, 568, 578
FriOI	GRGCYC	1 cut(s)	267
Fsp4HI	GCNGC	8 cut(s)	47, 73, 76, 161, 185, 197, 274, 482
FspBI	CTAG	1 cut(s)	656
GlaI	GCGC	7 cut(s)	58, 98, 159, 183, 195, 235, 408
GluI	GCNGC	8 cut(s)	47, 73, 76, 161, 185, 197, 274, 482
GsuI	CTGGAG	3 cut(s)	26, 516, 627
HaeII	RGCGCY	3 cut(s)	60, 197, 410
HaeIII	GGCC	3 cut(s)	206, 382, 758
HapII	CCGG	2 cut(s)	105, 329
HgaI	GACGC	1 cut(s)	293
HhaI	GCGC	7 cut(s)	59, 99, 160, 184, 196, 236, 409
Hin1I	GRCGYC	2 cut(s)	194, 285
Hin1II	CATG	4 cut(s)	349, 400, 473, 623
Hin6I	GCGC	7 cut(s)	57, 97, 158, 182, 194, 234, 407
HinP1I	GCGC	7 cut(s)	57, 97, 158, 182, 194, 234, 407
HincII	GTYRAC	1 cut(s)	313
HindII	GTYRAC	1 cut(s)	313
HindIII	AAGCTT	1 cut(s)	824
HinfI	GANTC	3 cut(s)	332, 443, 547
HpaII	CCGG	2 cut(s)	105, 329
HphI	GGTGA	1 cut(s)	764
Hpy166II	GTNNAC	3 cut(s)	16, 313, 818
Hpy188I	TCNGA	1 cut(s)	789
Hpy188III	TCNNGA	2 cut(s)	120, 329
Hpy8I	GTNNAC	3 cut(s)	16, 313, 818
Hpy99I	CGWCG	3 cut(s)	169, 205, 287
HpyAV	CCTTC	2 cut(s)	730, 775
HpyCH4III	ACNGT	2 cut(s)	528, 652
HpyCH4V	TGCA	4 cut(s)	84, 276, 424, 581
HpyF10VI	GCNNNNNNNGC	9 cut(s)	27, 81, 166, 175, 184, 193, 262, 415, 661
HpyF3I	CTNAG	3 cut(s)	60, 549, 805
Hsp92I	GRCGYC	2 cut(s)	194, 285
Hsp92II	CATG	4 cut(s)	349, 400, 473, 623
HspAI	GCGC	7 cut(s)	57, 97, 158, 182, 194, 234, 407
KasI	GGCGCC	1 cut(s)	193
Kpn2I	TCCGGA	1 cut(s)	328
Ksp22I	TGATCA	1 cut(s)	784
KspI	CCGCGG	1 cut(s)	188
Kzo9I	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
LmnI	GCTCC	1 cut(s)	26
Lsp1109I	GCAGC	4 cut(s)	33, 59, 260, 493
LweI	GCATC	2 cut(s)	108, 622
MaeI	CTAG	1 cut(s)	656
MaeIII	GTNAC	2 cut(s)	91, 776
MalI	GATC	7 cut(s)	124, 369, 475, 560, 575, 724, 786
MboI	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
MboII	GAAGA	5 cut(s)	229, 327, 368, 371, 590
MfeI	CAATTG	1 cut(s)	585
MhlI	GDGCHC	2 cut(s)	267, 533
MlsI	TGGCCA	1 cut(s)	382
MluCI	AATT	1 cut(s)	585
MluNI	TGGCCA	1 cut(s)	382
Mly113I	GGCGCC	1 cut(s)	194
MlyI	GAGTC	2 cut(s)	341, 541
MmeI	TCCRAC	1 cut(s)	48
Mox20I	TGGCCA	1 cut(s)	382
MroI	TCCGGA	1 cut(s)	328
MscI	TGGCCA	1 cut(s)	382
MseI	TTAA	3 cut(s)	5, 279, 612
MslI	CAYNNNNRTG	2 cut(s)	344, 401
Msp20I	TGGCCA	1 cut(s)	382
MspA1I	CMGCKG	5 cut(s)	46, 53, 72, 187, 484
MspI	CCGG	2 cut(s)	105, 329
MunI	CAATTG	1 cut(s)	585
MvnI	CGCG	5 cut(s)	97, 158, 182, 187, 256
MwoI	GCNNNNNNNGC	9 cut(s)	27, 81, 166, 175, 184, 193, 262, 415, 661
NarI	GGCGCC	1 cut(s)	194
NdeI	CATATG	1 cut(s)	701
NdeII	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
NlaIII	CATG	4 cut(s)	349, 400, 473, 623
NlaIV	GGNNCC	2 cut(s)	109, 195
NmuCI	GTSAC	2 cut(s)	91, 776
PfeI	GAWTC	1 cut(s)	443
PkrI	GCNGC	8 cut(s)	48, 74, 77, 162, 186, 198, 275, 483
PleI	GAGTC	2 cut(s)	340, 541
PluTI	GGCGCC	1 cut(s)	197
PpsI	GAGTC	2 cut(s)	340, 541
PshAI	GACNNNNGTC	1 cut(s)	714
Psp124BI	GAGCTC	1 cut(s)	267
PspN4I	GGNNCC	2 cut(s)	109, 195
PspPI	GGNCC	2 cut(s)	205, 709
PstNI	CAGNNNCTG	1 cut(s)	774
PvuII	CAGCTG	2 cut(s)	46, 53
RsaI	GTAC	4 cut(s)	173, 245, 649, 698
RsaNI	GTAC	4 cut(s)	172, 244, 648, 697
RseI	CAYNNNNRTG	2 cut(s)	344, 401
SacI	GAGCTC	1 cut(s)	267
SacII	CCGCGG	1 cut(s)	188
SaqAI	TTAA	3 cut(s)	5, 279, 612
SatI	GCNGC	8 cut(s)	47, 73, 76, 161, 185, 197, 274, 482
Sau3AI	GATC	7 cut(s)	122, 367, 473, 558, 573, 722, 784
Sau96I	GGNCC	2 cut(s)	205, 709
SchI	GAGTC	2 cut(s)	341, 541
SduI	GDGCHC	2 cut(s)	267, 533
SfaNI	GCATC	2 cut(s)	108, 622
SfcI	CTRYAG	1 cut(s)	673
SfoI	GGCGCC	1 cut(s)	195
Sfr303I	CCGCGG	1 cut(s)	188
SgrBI	CCGCGG	1 cut(s)	188
SinI	GGWCC	1 cut(s)	709
SmiMI	CAYNNNNRTG	2 cut(s)	344, 401
SmlI	CTYRAG	1 cut(s)	266
SmoI	CTYRAG	1 cut(s)	266
Sse9I	AATT	1 cut(s)	585
SsiI	CCGC	9 cut(s)	70, 75, 143, 161, 185, 187, 197, 224, 484
SspDI	GGCGCC	1 cut(s)	193
SspMI	CTAG	1 cut(s)	656
SstI	GAGCTC	1 cut(s)	267
TaaI	ACNGT	2 cut(s)	528, 652
TaqI	TCGA	2 cut(s)	376, 721
TasI	AATT	1 cut(s)	585
TauI	GCSGC	4 cut(s)	78, 163, 187, 199
TfiI	GAWTC	1 cut(s)	443
Tru1I	TTAA	3 cut(s)	5, 279, 612
Tru9I	TTAA	3 cut(s)	5, 279, 612
TscAI	CASTG	3 cut(s)	499, 531, 775
TseFI	GTSAC	2 cut(s)	91, 776
TseI	GCWGC	4 cut(s)	46, 72, 273, 481
Tsp45I	GTSAC	2 cut(s)	91, 776
TspDTI	ATGAA	3 cut(s)	311, 446, 608
TspGWI	ACGGA	3 cut(s)	225, 229, 551
TspRI	CASTG	3 cut(s)	499, 531, 775
VpaK11BI	GGWCC	1 cut(s)	709
XagI	CCTNNNNNAGG	1 cut(s)	723
XcmI	CCANNNNNNNNNTGG	1 cut(s)	50
XmiI	GTMKAC	1 cut(s)	15
XspI	CTAG	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.