RchiOBHm_Chr1g0373091

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000588
Physical Location & Seq
Forward (+)
61513983 .. 61516411
2429 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59702

Sequence Viewer

Length: 1065 bp
ATGTTAATGCTGGTTGACTGCTCCAACTGCCACACACCTCTCCAGCTGCCAGCTGGCGCTCAGGTAATCCGCTGCGGCTTATGCAACGCCGTCACCCGCATCGCCGGAACCTCCTCCTCCTCCCGATCTCTCCTTCCTCCTCATCCGCAATCCCCCCACGCGCCGCCGTCTCCGTACGGACACTCGCCTCCGCCGCCGGCCCACGGACGGAAGAGGGCGGTGCTGTGCGCCGTGTCGTACAAGGGAACGCGGCACGAGCTCAAGGGCTGCATTAACGACGCCAAGTGTATGAAGTATTTGCTGGTCAACAAGTTCAAGTTTCCGGAGTCTTCCATTGTCATGCTCACAGAAGAAGAAGCTGATCCTTTTCGACGGCCAACCAAACAAAACATGAGAATGGCACTGTTTTGGCTTGTGCAAGGTTGTCAAGCAGGGGATTCTTTGGTGTTTCATTATTCGGGGCATGGATCACAGCAGCGGAACTACACTGGAGACGAGGTGGATGGATATGATGAGACACTGTGTCCCACGGATTATGAGACTCAGGGGATGATCGTGGATGATGAGATCAATGCAACAATTGTTAGACCTCTTCCTGCTGGAGTTAAACTTCATGCTATTGTAGATGCTTGCCATAGTGGTACTGTGCTAGATTTGCCATTTCTCTGTAGAATGGACAGAAATGGGAGGTACATATGGGAGGACCATCGTCCTCGATCAGGTGTTTGGAAGGGAACAAGTGGTGGTGAGGCCATTTCCTTCAGTGGCTGCGACGATGATCAGACCTCTGCTGATACCTCAGCTCTGTCAAAGATTACCTCAACTGGTGCCATGACTTATGCTTTCATCCAAGCCATAGAGCGCGGACATGCAACCACATACGGAAACATGTTGAATGCAATGCGATCCACCATCCGTAACACTGATGGTGGTGCTGGAGGCGGTATTGTGACAACCCTTATCTCCATGCTTTTGACAGGAGGGAGTCTCGGAAGGTTGAGACAGGAACCACAGCTAACTGCCGATCAACAATATGATGTGTATACAAAACCTTTCTCCCTATAG

Protein Analysis

354

Amino Acids

38.53

Weight (kDa)

6.85

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 7 - 31 2.6e-08 LSD1 zinc finger
Peptidase_C14 PF00656 71 - 341 3.7e-36 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 827
AccI GTMKAC 1 cut(s) 1043
AccII CGCG 3 cut(s) 161, 250, 864
AccIII TCCGGA 1 cut(s) 322
AclWI GGATC 3 cut(s) 356, 475, 900
AcoI YGGCCR 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 745
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 176, 239, 643, 692
AfiI CCNNNNNNNGG 3 cut(s) 203, 207, 719
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 2 cut(s) 316, 895
AhdI GACNNNNNGTC 1 cut(s) 522
AluBI AGCT 6 cut(s) 46, 53, 259, 359, 803, 1015
AluI AGCT 6 cut(s) 46, 53, 259, 359, 803, 1015
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 6 cut(s) 174, 486, 509, 533, 992, 994
AlwI GGATC 3 cut(s) 356, 475, 900
AlwNI CAGNNNCTG 1 cut(s) 768
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 3 cut(s) 198, 374, 750
ApeKI GCWGC 5 cut(s) 46, 72, 267, 475, 768
AspLEI GCGC 4 cut(s) 59, 163, 230, 864
AspS9I GGNCC 2 cut(s) 199, 703
AsuHPI GGTGA 2 cut(s) 85, 758
AvaII GGWCC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 261
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 799
BbvI GCAGC 5 cut(s) 33, 59, 254, 487, 755
BccI CCATC 4 cut(s) 497, 714, 920, 920
BceAI ACGGC 4 cut(s) 74, 151, 215, 389
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 6 cut(s) 174, 486, 509, 533, 992, 994
BfaI CTAG 1 cut(s) 650
BfmI CTRYAG 2 cut(s) 667, 1061
BfoI RGCGCY 1 cut(s) 60
BisI GCNGC 9 cut(s) 47, 73, 76, 164, 194, 251, 268, 476, 769
BlsI GCNGC 9 cut(s) 48, 74, 77, 165, 195, 252, 269, 477, 770
Bme18I GGWCC 1 cut(s) 703
BmeRI GACNNNNNGTC 1 cut(s) 522
BmgT120I GGNCC 2 cut(s) 199, 703
BmiI GGNNCC 3 cut(s) 109, 829, 1008
BmsI GCATC 2 cut(s) 108, 616
BoxI GACNNNNGTC 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 972, 1004
BpmI CTGGAG 4 cut(s) 26, 510, 621, 957
Bpu10I CCTNAGC 2 cut(s) 60, 799
BpuEI CTTGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 202, 528
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 207, 719
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 2 cut(s) 493, 829
Bse3DI GCAATG 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 202, 528
BseGI GGATG 6 cut(s) 142, 508, 555, 565, 846, 912
BseLI CCNNNNNNNGG 3 cut(s) 203, 207, 719
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 3 cut(s) 74, 557, 813
BseNI ACTGG 2 cut(s) 493, 829
BseRI GAGGAG 4 cut(s) 103, 106, 109, 129
BseXI GCAGC 5 cut(s) 33, 59, 254, 487, 755
Bsh1236I CGCG 3 cut(s) 161, 250, 864
BshFI GGCC 3 cut(s) 200, 376, 752
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 105, 197, 323
BsiWI CGTACG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 3 cut(s) 203, 207, 719
BsmAI GTCTC 6 cut(s) 174, 486, 509, 533, 992, 994
BsmBI CGTCTC 2 cut(s) 174, 486
BsmFI GGGAC 1 cut(s) 510
BsmI GAATGC 1 cut(s) 901
BsnI GGCC 3 cut(s) 200, 376, 752
Bsp1286I GDGCHC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
BspANI GGCC 3 cut(s) 200, 376, 752
BspCNI CTCAG 3 cut(s) 73, 556, 812
BspEI TCCGGA 1 cut(s) 322
BspFNI CGCG 3 cut(s) 161, 250, 864
BspLI GGNNCC 3 cut(s) 109, 829, 1008
BspPI GGATC 3 cut(s) 356, 475, 900
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 906
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 2 cut(s) 493, 829
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 202, 528
BssMI GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
BssNAI GTATAC 1 cut(s) 1044
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 254
Bst1107I GTATAC 1 cut(s) 1044
Bst2BI CACGAG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 405, 522, 646
Bst6I CTCTTC 2 cut(s) 206, 597
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 4 cut(s) 51, 55, 198, 631
BstDEI CTNAG 3 cut(s) 60, 543, 799
BstDSI CCRYGG 2 cut(s) 202, 528
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 6 cut(s) 142, 508, 555, 565, 846, 912
BstFNI CGCG 3 cut(s) 161, 250, 864
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 4 cut(s) 59, 163, 230, 864
BstKTI GATC 9 cut(s) 128, 364, 470, 555, 570, 719, 781, 908, 1027
BstMAI GTCTC 6 cut(s) 174, 486, 509, 533, 992, 994
BstMBI GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
BstMWI GCNNNNNNNGC 5 cut(s) 27, 81, 193, 256, 655
BstNSI RCATGY 2 cut(s) 872, 892
BstPAI GACNNNNGTC 1 cut(s) 708
BstSFI CTRYAG 2 cut(s) 667, 1061
BstUI CGCG 3 cut(s) 161, 250, 864
BstV1I GCAGC 5 cut(s) 33, 59, 254, 487, 755
BstV2I GAAGAC 1 cut(s) 321
BstZ17I GTATAC 1 cut(s) 1044
BsuRI GGCC 3 cut(s) 200, 376, 752
BtgI CCRYGG 2 cut(s) 202, 528
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 6 cut(s) 142, 508, 555, 565, 846, 912
BtsIMutI CAGTG 5 cut(s) 401, 486, 518, 769, 921
Cac8I GCNNGC 4 cut(s) 51, 55, 198, 631
CaiI CAGNNNCTG 1 cut(s) 768
CfoI GCGC 4 cut(s) 59, 163, 230, 864
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 199, 703
CseI GACGC 1 cut(s) 287
Csp6I GTAC 4 cut(s) 175, 238, 642, 691
CviAII CATG 8 cut(s) 340, 391, 464, 614, 832, 869, 889, 967
CviQI GTAC 4 cut(s) 175, 238, 642, 691
DdeI CTNAG 3 cut(s) 60, 543, 799
DpnI GATC 9 cut(s) 127, 363, 469, 554, 569, 718, 780, 907, 1026
DpnII GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 206, 597
Eam1105I GACNNNNNGTC 1 cut(s) 522
EarI CTCTTC 2 cut(s) 206, 597
EciI GGCGGA 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 703
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 745
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 261
Esp3I CGTCTC 2 cut(s) 174, 486
FaeI CATG 8 cut(s) 343, 394, 467, 617, 835, 872, 892, 970
FaqI GGGAC 1 cut(s) 510
FatI CATG 8 cut(s) 339, 390, 463, 613, 831, 868, 888, 966
FauI CCCGC 1 cut(s) 104
FauNDI CATATG 1 cut(s) 695
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 9 cut(s) 47, 73, 76, 164, 194, 251, 268, 476, 769
FokI GGATG 6 cut(s) 129, 515, 562, 572, 833, 899
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 9 cut(s) 47, 73, 76, 164, 194, 251, 268, 476, 769
FspBI CTAG 1 cut(s) 650
GlaI GCGC 4 cut(s) 58, 162, 229, 863
GluI GCNGC 9 cut(s) 47, 73, 76, 164, 194, 251, 268, 476, 769
GsuI CTGGAG 4 cut(s) 26, 510, 621, 957
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 200, 376, 752
HapII CCGG 3 cut(s) 105, 197, 323
HgaI GACGC 1 cut(s) 287
HhaI GCGC 4 cut(s) 59, 163, 230, 864
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 343, 394, 467, 617, 835, 872, 892, 970
Hin6I GCGC 4 cut(s) 57, 161, 228, 862
HinP1I GCGC 4 cut(s) 57, 161, 228, 862
HincII GTYRAC 2 cut(s) 16, 307
HindII GTYRAC 2 cut(s) 16, 307
HinfI GANTC 4 cut(s) 326, 437, 541, 985
HpaII CCGG 3 cut(s) 105, 197, 323
HphI GGTGA 2 cut(s) 85, 758
Hpy166II GTNNAC 3 cut(s) 16, 307, 1044
Hpy188I TCNGA 2 cut(s) 783, 992
Hpy188III TCNNGA 2 cut(s) 123, 323
Hpy8I GTNNAC 3 cut(s) 16, 307, 1044
Hpy99I CGWCG 3 cut(s) 281, 375, 776
HpyAV CCTTC 4 cut(s) 143, 724, 769, 987
HpyCH4III ACNGT 3 cut(s) 405, 522, 646
HpyCH4V TGCA 6 cut(s) 84, 270, 418, 575, 872, 899
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 81, 193, 256, 655
HpyF3I CTNAG 3 cut(s) 60, 543, 799
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 343, 394, 467, 617, 835, 872, 892, 970
HspAI GCGC 4 cut(s) 57, 161, 228, 862
Kpn2I TCCGGA 1 cut(s) 322
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
LmnI GCTCC 1 cut(s) 26
Lsp1109I GCAGC 5 cut(s) 33, 59, 254, 487, 755
LweI GCATC 2 cut(s) 108, 616
MaeI CTAG 1 cut(s) 650
MaeIII GTNAC 3 cut(s) 91, 917, 949
MalI GATC 9 cut(s) 127, 363, 469, 554, 569, 718, 780, 907, 1026
MboI GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
MboII GAAGA 5 cut(s) 223, 321, 362, 365, 584
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 1 cut(s) 579
MlyI GAGTC 3 cut(s) 335, 535, 994
MmeI TCCRAC 1 cut(s) 48
MroI TCCGGA 1 cut(s) 322
MroNI GCCGGC 1 cut(s) 196
MseI TTAA 3 cut(s) 5, 273, 606
MslI CAYNNNNRTG 2 cut(s) 338, 395
MspA1I CMGCKG 4 cut(s) 46, 53, 72, 478
MspI CCGG 3 cut(s) 105, 197, 323
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 901
MvnI CGCG 3 cut(s) 161, 250, 864
MwoI GCNNNNNNNGC 5 cut(s) 27, 81, 193, 256, 655
NaeI GCCGGC 1 cut(s) 198
NdeI CATATG 1 cut(s) 695
NdeII GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 8 cut(s) 343, 394, 467, 617, 835, 872, 892, 970
NlaIV GGNNCC 3 cut(s) 109, 829, 1008
NmuCI GTSAC 2 cut(s) 91, 949
NspI RCATGY 2 cut(s) 872, 892
PciI ACATGT 1 cut(s) 888
PctI GAATGC 1 cut(s) 901
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 437
Pfl23II CGTACG 1 cut(s) 174
PkrI GCNGC 9 cut(s) 48, 74, 77, 165, 195, 252, 269, 477, 770
PleI GAGTC 3 cut(s) 334, 535, 993
PpsI GAGTC 3 cut(s) 334, 535, 993
PscI ACATGT 1 cut(s) 888
PshAI GACNNNNGTC 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 261
PspLI CGTACG 1 cut(s) 174
PspN4I GGNNCC 3 cut(s) 109, 829, 1008
PspPI GGNCC 2 cut(s) 199, 703
PstNI CAGNNNCTG 1 cut(s) 768
PvuII CAGCTG 2 cut(s) 46, 53
RsaI GTAC 4 cut(s) 176, 239, 643, 692
RsaNI GTAC 4 cut(s) 175, 238, 642, 691
RseI CAYNNNNRTG 2 cut(s) 338, 395
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 5, 273, 606
SatI GCNGC 9 cut(s) 47, 73, 76, 164, 194, 251, 268, 476, 769
Sau3AI GATC 9 cut(s) 125, 361, 467, 552, 567, 716, 778, 905, 1024
Sau96I GGNCC 2 cut(s) 199, 703
SchI GAGTC 3 cut(s) 335, 535, 994
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 2 cut(s) 108, 616
SfcI CTRYAG 2 cut(s) 667, 1061
SinI GGWCC 1 cut(s) 703
SmiMI CAYNNNNRTG 2 cut(s) 338, 395
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 579
SspMI CTAG 1 cut(s) 650
SstI GAGCTC 1 cut(s) 261
TaaI ACNGT 3 cut(s) 405, 522, 646
TaqI TCGA 2 cut(s) 370, 715
TasI AATT 1 cut(s) 579
TauI GCSGC 4 cut(s) 78, 166, 196, 253
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 3 cut(s) 5, 273, 606
Tru9I TTAA 3 cut(s) 5, 273, 606
TscAI CASTG 5 cut(s) 408, 493, 525, 769, 928
TseFI GTSAC 2 cut(s) 91, 949
TseI GCWGC 5 cut(s) 46, 72, 267, 475, 768
Tsp45I GTSAC 2 cut(s) 91, 949
TspDTI ATGAA 4 cut(s) 305, 440, 602, 835
TspGWI ACGGA 7 cut(s) 162, 192, 219, 223, 545, 897, 905
TspRI CASTG 5 cut(s) 408, 493, 525, 769, 928
VpaK11BI GGWCC 1 cut(s) 703
XagI CCTNNNNNAGG 1 cut(s) 717
XceI RCATGY 2 cut(s) 872, 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 1043
XspI CTAG 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.