Rh1CG176200

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000588
Physical Location & Seq
Reverse (-)
38800769 .. 38804050
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG176200.1

Sequence Viewer

Length: 1035 bp
ATGTACAGCAATATGCTGGTGAACTGCTCCAACTGCCACACCCCGCTCCAGCTTCCGTCGGGCGCCGAGTCGATCCGCTGCGCCCTCTGCCAGGCCGTCACCCTAATCGCGGACCCACGCTCCCTACACTCACTTCCCTCCTCCCACGCGCTCCCACCTCCCTCTCCTTACGGCCACGCGCCTCCCCCTCCTCCCTCGCCCTACAACCACGCGCCGCCGGGGCCTCCGCCGAGCGTGCACGGCCGGAAGAAGGCGGTGATCTGCGGGATATCGTATAAATACTCGAGGCATGAGCTCAAGGGTTGCATCAATGACGCCAAATGCATGCGGTATCTCCTCATCAACAAGTTCAAGTTTCCCGACGAATCCATTGTCATGCTCACTGAAGAAGAAACTCACCCAGCTAAGATTCCAAATAAATACAACATTAGAATGGCATTACATTGGCTTGTACAAGGATGCCAACCAGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGGCAGAGGGCTTATCATGATGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACCCAGGGTATGATTGTCGATGATGAGATAAATGCAACAATTGTTAAGCCCCTTCCACATGGAGTCAAGCTTCATGCAATTATAGACTCCTGTCATAGTGGCACTGTACTGGATTTGCCATTCCTTTGCAGAATGGACAGGGGTGGAAAATATGTATGGGAGGATCATCGCCCTCGATCAGGCATGTGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGACGATCAAACCTCTGCTGATACATCAGTAAGTTTGACACGGAAAAAAAATGTCTCGAGTTCAGTAATGTTTATAGCTTCAATTTCCACACACATGATTGATACTTTTTGGGGTACAGGCTCTATCCAAGATCACATCAACCGGTGCCATGACTTTCTGCTTCATCCAAGCAATCGAGCGTGGACAGGCAGCCACATATGGGAGCATACTCAATTCTATGCGTAA

Protein Analysis

344

Amino Acids

38.23

Weight (kDa)

6.5

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 9 - 33 1.2e-10 LSD1 zinc finger
Peptidase_C14 PF00656 82 - 316 2.9e-30 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 954
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 4 cut(s) 110, 149, 179, 212
AciI CCGC 8 cut(s) 44, 76, 110, 215, 227, 254, 264, 328
AclWI GGATC 2 cut(s) 67, 747
AcoI YGGCCR 2 cut(s) 172, 241
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 2 cut(s) 63, 315
AfaI GTAC 4 cut(s) 5, 453, 684, 925
AfiI CCNNNNNNNGG 5 cut(s) 91, 109, 250, 755, 1009
AgeI ACCGGT 1 cut(s) 951
AgsI TTSAA 2 cut(s) 352, 891
AjnI CCWGG 3 cut(s) 90, 466, 579
AluBI AGCT 5 cut(s) 52, 295, 404, 646, 887
AluI AGCT 5 cut(s) 52, 295, 404, 646, 887
Alw21I GWGCWC 2 cut(s) 240, 297
Alw26I GTCTC 1 cut(s) 868
Alw44I GTGCAC 1 cut(s) 236
AlwI GGATC 2 cut(s) 67, 747
Ama87I CYCGRG 2 cut(s) 283, 865
AoxI GGCC 4 cut(s) 93, 172, 221, 241
ApaLI GTGCAC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 78, 999
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AsiGI ACCGGT 1 cut(s) 951
AspLEI GCGC 5 cut(s) 65, 83, 151, 181, 214
AspS9I GGNCC 2 cut(s) 112, 221
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 5 cut(s) 31, 91, 268, 389, 482
AsuII TTCGAA 1 cut(s) 573
AvaI CYCGRG 2 cut(s) 283, 865
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 240
BaeI ACNNNNGTAYC 2 cut(s) 822, 855
BanI GGYRCC 2 cut(s) 62, 954
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 240, 297
BbvI GCAGC 2 cut(s) 65, 1011
BccI CCATC 1 cut(s) 533
BceAI ACGGC 4 cut(s) 80, 187, 256, 524
BciT130I CCWGG 3 cut(s) 92, 468, 581
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 868
BfoI RGCGCY 1 cut(s) 66
BglI GCCNNNNNGGC 1 cut(s) 220
BisI GCNGC 3 cut(s) 79, 215, 1000
BlsI GCNGC 3 cut(s) 80, 216, 1001
Bme1390I CCNGG 4 cut(s) 92, 219, 468, 581
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 2 cut(s) 283, 865
BmgT120I GGNCC 2 cut(s) 112, 221
BmiI GGNNCC 4 cut(s) 64, 114, 222, 956
BmrFI CCNGG 4 cut(s) 92, 219, 468, 581
BmsI GCATC 2 cut(s) 315, 449
BoxI GACNNNNGTC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 813
BsaHI GRCGYC 2 cut(s) 63, 315
BsaJI CCNNGG 3 cut(s) 218, 579, 580
BsaWI WCCGGW 1 cut(s) 951
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 109, 250, 755, 1009
Bse118I RCCGGY 1 cut(s) 951
Bse1I ACTGG 1 cut(s) 690
Bse8I GATNNNNATC 1 cut(s) 813
BseBI CCWGG 3 cut(s) 92, 468, 581
BseDI CCNNGG 3 cut(s) 218, 579, 580
BseGI GGATG 2 cut(s) 464, 973
BseJI GATNNNNATC 1 cut(s) 813
BseLI CCNNNNNNNGG 5 cut(s) 91, 109, 250, 755, 1009
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 3 cut(s) 130, 180, 326
BseSI GKGCMC 1 cut(s) 240
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 65, 1011
BseYI CCCAGC 1 cut(s) 400
Bsh1236I CGCG 4 cut(s) 110, 149, 179, 212
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 95, 174, 223, 243
BshNI GGYRCC 2 cut(s) 62, 954
BshTI ACCGGT 1 cut(s) 951
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 240, 297
BsiHKCI CYCGRG 2 cut(s) 283, 865
BsiSI CCGG 3 cut(s) 218, 244, 952
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 5 cut(s) 91, 109, 250, 755, 1009
BsmAI GTCTC 1 cut(s) 868
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 4 cut(s) 95, 174, 223, 243
BsoBI CYCGRG 2 cut(s) 283, 865
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 240, 297
Bsp1407I TGTACA 2 cut(s) 3, 451
Bsp143I GATC 6 cut(s) 72, 258, 739, 752, 814, 940
BspACI CCGC 8 cut(s) 44, 76, 110, 215, 227, 254, 264, 328
BspANI GGCC 4 cut(s) 95, 174, 223, 243
BspFNI CGCG 4 cut(s) 110, 149, 179, 212
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 4 cut(s) 64, 114, 222, 956
BspPI GGATC 2 cut(s) 67, 747
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 2 cut(s) 62, 954
BsrBI CCGCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 951
BsrGI TGTACA 2 cut(s) 3, 451
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 1 cut(s) 951
BssECI CCNNGG 3 cut(s) 218, 579, 580
BssMI GATC 6 cut(s) 72, 258, 739, 752, 814, 940
BssNI GRCGYC 2 cut(s) 63, 315
Bst2UI CCWGG 3 cut(s) 92, 468, 581
Bst4CI ACNGT 1 cut(s) 682
BstACI GRCGYC 2 cut(s) 63, 315
BstAUI TGTACA 2 cut(s) 3, 451
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 236, 326
BstDEI CTNAG 1 cut(s) 405
BstENI CCTNNNNNAGG 2 cut(s) 89, 753
BstF5I GGATG 2 cut(s) 464, 973
BstFNI CGCG 4 cut(s) 110, 149, 179, 212
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 5 cut(s) 65, 83, 151, 181, 214
BstKTI GATC 6 cut(s) 75, 261, 742, 755, 817, 943
BstMAI GTCTC 1 cut(s) 868
BstMBI GATC 6 cut(s) 72, 258, 739, 752, 814, 940
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 33, 87, 220, 235, 240
BstNI CCWGG 3 cut(s) 92, 468, 581
BstNSI RCATGY 2 cut(s) 328, 763
BstPAI GACNNNNGTC 1 cut(s) 666
BstSCI CCNGG 4 cut(s) 90, 217, 466, 579
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 4 cut(s) 110, 149, 179, 212
BstV1I GCAGC 2 cut(s) 65, 1011
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 4 cut(s) 95, 174, 223, 243
BtgZI GCGATG 1 cut(s) 728
BtsCI GGATG 2 cut(s) 464, 973
BtsIMutI CAGTG 3 cut(s) 381, 678, 805
Cac8I GCNNGC 2 cut(s) 236, 326
CciI TCATGA 1 cut(s) 523
CfoI GCGC 5 cut(s) 65, 83, 151, 181, 214
Cfr10I RCCGGY 1 cut(s) 951
Cfr13I GGNCC 2 cut(s) 112, 221
CseI GACGC 1 cut(s) 323
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 4 cut(s) 4, 452, 683, 924
CspAI ACCGGT 1 cut(s) 951
CviQI GTAC 4 cut(s) 4, 452, 683, 924
DdeI CTNAG 1 cut(s) 405
DinI GGCGCC 1 cut(s) 64
DpnI GATC 6 cut(s) 74, 260, 741, 754, 816, 942
DpnII GATC 6 cut(s) 72, 258, 739, 752, 814, 940
EaeI YGGCCR 2 cut(s) 172, 241
EagI CGGCCG 1 cut(s) 241
EciI GGCGGA 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 295
EclXI CGGCCG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 405
Eco88I CYCGRG 2 cut(s) 283, 865
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 2 cut(s) 89, 753
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 3 cut(s) 90, 466, 579
EcoRV GATATC 1 cut(s) 270
EcoT22I ATGCAT 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaqI GGGAC 1 cut(s) 546
FauI CCCGC 2 cut(s) 51, 257
FauNDI CATATG 1 cut(s) 1007
Fnu4HI GCNGC 3 cut(s) 79, 215, 1000
FokI GGATG 2 cut(s) 471, 960
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 3 cut(s) 79, 215, 1000
GlaI GCGC 5 cut(s) 64, 82, 150, 180, 213
GluI GCNGC 3 cut(s) 79, 215, 1000
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 32
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 95, 174, 223, 243
HapII CCGG 3 cut(s) 218, 244, 952
HgaI GACGC 1 cut(s) 323
HhaI GCGC 5 cut(s) 65, 83, 151, 181, 214
Hin1I GRCGYC 2 cut(s) 63, 315
Hin6I GCGC 5 cut(s) 63, 81, 149, 179, 212
HinP1I GCGC 5 cut(s) 63, 81, 149, 179, 212
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 6 cut(s) 68, 365, 409, 473, 639, 662
HpaII CCGG 3 cut(s) 218, 244, 952
HphI GGTGA 5 cut(s) 31, 91, 268, 389, 482
Hpy166II GTNNAC 4 cut(s) 22, 238, 506, 993
Hpy188III TCNNGA 3 cut(s) 359, 524, 865
Hpy8I GTNNAC 4 cut(s) 22, 238, 506, 993
Hpy99I CGWCG 2 cut(s) 61, 365
HpyAV CCTTC 2 cut(s) 244, 638
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4V TGCA 6 cut(s) 238, 306, 324, 611, 653, 705
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 87, 220, 235, 240
HpyF3I CTNAG 1 cut(s) 405
Hsp92I GRCGYC 2 cut(s) 63, 315
HspAI GCGC 5 cut(s) 63, 81, 149, 179, 212
KasI GGCGCC 1 cut(s) 62
Kzo9I GATC 6 cut(s) 72, 258, 739, 752, 814, 940
LmnI GCTCC 5 cut(s) 32, 51, 125, 156, 1012
Lsp1109I GCAGC 2 cut(s) 65, 1011
LweI GCATC 2 cut(s) 315, 449
MabI ACCWGGT 1 cut(s) 466
MaeIII GTNAC 2 cut(s) 97, 470
MalI GATC 6 cut(s) 74, 260, 741, 754, 816, 942
MbiI CCGCTC 1 cut(s) 46
MboI GATC 6 cut(s) 72, 258, 739, 752, 814, 940
MboII GAAGA 3 cut(s) 259, 398, 401
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 2 cut(s) 240, 297
MluCI AATT 4 cut(s) 615, 654, 891, 1022
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 4 cut(s) 77, 467, 648, 656
MmeI TCCRAC 1 cut(s) 54
Mph1103I ATGCAT 1 cut(s) 326
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 4 cut(s) 374, 431, 528, 902
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 3 cut(s) 218, 244, 952
MspR9I CCNGG 4 cut(s) 92, 219, 468, 581
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 3 cut(s) 92, 468, 581
MvnI CGCG 4 cut(s) 110, 149, 179, 212
MwoI GCNNNNNNNGC 5 cut(s) 33, 87, 220, 235, 240
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 1007
NdeII GATC 6 cut(s) 72, 258, 739, 752, 814, 940
NlaIV GGNNCC 4 cut(s) 64, 114, 222, 956
NmeAIII GCCGAG 2 cut(s) 91, 255
NmuCI GTSAC 2 cut(s) 97, 470
NsiI ATGCAT 1 cut(s) 326
NspI RCATGY 2 cut(s) 328, 763
NspV TTCGAA 1 cut(s) 573
PaeI GCATGC 1 cut(s) 328
PaeR7I CTCGAG 2 cut(s) 283, 865
PagI TCATGA 1 cut(s) 523
PasI CCCWGGG 1 cut(s) 580
PfeI GAWTC 2 cut(s) 365, 409
PinAI ACCGGT 1 cut(s) 951
PkrI GCNGC 3 cut(s) 80, 216, 1001
PleI GAGTC 4 cut(s) 76, 467, 647, 656
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 4 cut(s) 76, 467, 647, 656
PshAI GACNNNNGTC 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 3 cut(s) 90, 466, 579
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 3 cut(s) 90, 466, 579
PspN4I GGNNCC 4 cut(s) 64, 114, 222, 956
PspPI GGNCC 2 cut(s) 112, 221
PspXI VCTCGAGB 1 cut(s) 283
RsaI GTAC 4 cut(s) 5, 453, 684, 925
RsaNI GTAC 4 cut(s) 4, 452, 683, 924
RseI CAYNNNNRTG 4 cut(s) 374, 431, 528, 902
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 79, 215, 1000
Sau3AI GATC 6 cut(s) 72, 258, 739, 752, 814, 940
Sau96I GGNCC 2 cut(s) 112, 221
SchI GAGTC 4 cut(s) 77, 467, 648, 656
ScrFI CCNGG 4 cut(s) 92, 219, 468, 581
SduI GDGCHC 2 cut(s) 240, 297
SetI ASST 8 cut(s) 54, 160, 297, 406, 472, 648, 824, 889
SexAI ACCWGGT 1 cut(s) 466
SfaNI GCATC 2 cut(s) 315, 449
SfoI GGCGCC 1 cut(s) 64
Sfr274I CTCGAG 2 cut(s) 283, 865
SfuI TTCGAA 1 cut(s) 573
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 2 cut(s) 283, 865
SmiMI CAYNNNNRTG 4 cut(s) 374, 431, 528, 902
SmlI CTYRAG 3 cut(s) 283, 296, 865
SmoI CTYRAG 3 cut(s) 283, 296, 865
SphI GCATGC 1 cut(s) 328
Sse9I AATT 4 cut(s) 615, 654, 891, 1022
SsiI CCGC 8 cut(s) 44, 76, 110, 215, 227, 254, 264, 328
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 4 cut(s) 90, 217, 466, 579
TaaI ACNGT 1 cut(s) 682
TaqI TCGA 7 cut(s) 71, 284, 573, 594, 751, 866, 985
TasI AATT 4 cut(s) 615, 654, 891, 1022
TatI WGTACW 3 cut(s) 3, 451, 682
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 2 cut(s) 365, 409
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 3 cut(s) 388, 685, 805
TseFI GTSAC 2 cut(s) 97, 470
TseI GCWGC 2 cut(s) 78, 999
Tsp45I GTSAC 2 cut(s) 97, 470
TspDTI ATGAA 4 cut(s) 546, 564, 638, 962
TspGWI ACGGA 2 cut(s) 45, 865
TspRI CASTG 3 cut(s) 388, 685, 805
VneI GTGCAC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 112
XagI CCTNNNNNAGG 2 cut(s) 89, 753
XceI RCATGY 2 cut(s) 328, 763
XhoI CTCGAG 2 cut(s) 283, 865
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.