RLG00000028877

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000588
Physical Location & Seq
Forward (+)
29606483 .. 29610882
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028877

Sequence Viewer

Length: 1197 bp
ATGATGATGAGCAATTATGGAGGCCAACGACTACTAGTGGTCGACTGTTGCTATTGCCAGACCCAAATCCAGTTGCCTTCTGTGAGTCCGATATACATGGTCGGCACATCCGTCCGCTGCGGCAGCTGCTATGCCAGTACCCGCGTCGCCTCTCCGGGCTTCCCCCGATCCCCCTATAGTGCTGTACCCTACTATGTCCCTGTAAACCCACCACCACACGCCGGCTTCCCCCACAGCTCACCAGGACCTCAGCCGAACGTGCACAGGCCGAAGAAGGCGGTGATCTGCGGGATATCGTATAGATACTCGAGGTATGAGCTCAAAGGTTGCATCAATGACGCCAAAAACATGCGGTATCTCCTCACGAGCAAGTTCAAGTTTCCAGAAGATTCCATTTGCATGCTCACTGAAGAAGAAACTCACCCGTATAAGATTCCATATAAAAACAACATTAGAATGGCATTAGATTGGCTTGTACAAGGATGCCAACCAGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGGCAGTTGGATTATGATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACCCAGGGTATGATTGATGATGATGAGATAAATGCAGCAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTCTTGTCATAGTGGCACCATTCTGGATTTGCCATTCCTTTGTAGAATGAACATGGGTGGAATATATGTATGGGAGGATCATCGCCCTCCATCAGGCGTATGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGACTATCAAACGTCTGCGGAAACATCAGCTCTATCCAGGATCACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCAATCGAGCATGGACAGGCAGCCACATATGGGAGCTTACTCAATTCTATGCGTAGTACCATTCGAAGTATGGGTTCTGCTGGTGGTGGTAGTGGTGGTGCTGTTACATCTCTTGCCGGCATGCTTCTAATAGGCGGAAGTGTTGGTGGTGGTAGGCTAAGACAGTCAAGCTACTCGATTGGGTGCAGCAGTGGCTGGACTGTGGCTAGGGCGCGACGCTTGGAGGACAACCTAGTCAGGCTGCGGGACGGGAGTGGAACGATGGTGGTCGACCACTGGAGGTAA

Protein Analysis

399

Amino Acids

43.35

Weight (kDa)

6.62

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 91 - 326 7.3e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1145
AccB1I GGYRCC 2 cut(s) 701, 887
AccI GTMKAC 2 cut(s) 42, 1182
AccII CGCG 2 cut(s) 144, 1126
AciI CCGC 9 cut(s) 115, 120, 142, 278, 288, 352, 851, 1047, 1156
AclWI GGATC 3 cut(s) 162, 771, 881
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 4 cut(s) 139, 186, 477, 970
AfiI CCNNNNNNNGG 3 cut(s) 221, 779, 942
AgsI TTSAA 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 4 cut(s) 241, 490, 603, 869
AluBI AGCT 7 cut(s) 126, 237, 319, 670, 863, 948, 1083
AluI AGCT 7 cut(s) 126, 237, 319, 670, 863, 948, 1083
Alw21I GWGCWC 2 cut(s) 264, 321
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 3 cut(s) 162, 771, 881
AlwNI CAGNNNCTG 1 cut(s) 1107
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 3 cut(s) 22, 266, 647
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 7 cut(s) 117, 123, 126, 635, 932, 1098, 1153
AspLEI GCGC 1 cut(s) 1126
AspS9I GGNCC 2 cut(s) 245, 648
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 5 cut(s) 231, 292, 413, 506, 563
AsuII TTCGAA 2 cut(s) 597, 976
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 701, 887
BanII GRGCYC 1 cut(s) 321
BauI CACGAG 1 cut(s) 364
Bbv12I GWGCWC 2 cut(s) 264, 321
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 7 cut(s) 104, 113, 135, 647, 944, 1110, 1140
BccI CCATC 4 cut(s) 542, 557, 784, 1168
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 4 cut(s) 243, 492, 605, 871
BcnI CCSGG 1 cut(s) 156
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 3 cut(s) 35, 1119, 1145
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 8 cut(s) 118, 121, 124, 127, 636, 933, 1099, 1154
BlsI GCNGC 8 cut(s) 119, 122, 125, 128, 637, 934, 1100, 1155
Bme1390I CCNGG 5 cut(s) 156, 243, 492, 605, 871
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 245, 648
BmiI GGNNCC 2 cut(s) 703, 889
BmrFI CCNGG 5 cut(s) 156, 243, 492, 605, 871
BmsI GCATC 2 cut(s) 339, 473
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 2 cut(s) 597, 976
BpuMI CCSGG 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 603, 604
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 779, 942
Bse118I RCCGGY 2 cut(s) 221, 1028
Bse1I ACTGG 3 cut(s) 70, 135, 1193
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 4 cut(s) 243, 492, 605, 871
BseDI CCNNGG 2 cut(s) 603, 604
BseGI GGATG 3 cut(s) 107, 488, 906
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 3 cut(s) 221, 779, 942
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 3 cut(s) 70, 135, 1193
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 7 cut(s) 104, 113, 135, 647, 944, 1110, 1140
BsgI GTGCAG 1 cut(s) 1117
Bsh1236I CGCG 2 cut(s) 144, 1126
BshFI GGCC 3 cut(s) 24, 268, 649
BshNI GGYRCC 2 cut(s) 701, 887
BsiHKAI GWGCWC 2 cut(s) 264, 321
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 3 cut(s) 155, 222, 1029
BslFI GGGAC 3 cut(s) 182, 570, 1172
BslI CCNNNNNNNGG 3 cut(s) 221, 779, 942
BsmFI GGGAC 3 cut(s) 182, 570, 1172
BsnI GGCC 3 cut(s) 24, 268, 649
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 2 cut(s) 597, 976
Bsp1286I GDGCHC 2 cut(s) 264, 321
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 4 cut(s) 167, 282, 763, 873
BspACI CCGC 9 cut(s) 115, 120, 142, 278, 288, 352, 851, 1047, 1156
BspANI GGCC 3 cut(s) 24, 268, 649
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 2 cut(s) 144, 1126
BspLI GGNNCC 2 cut(s) 703, 889
BspPI GGATC 3 cut(s) 162, 771, 881
BspT104I TTCGAA 2 cut(s) 597, 976
BspT107I GGYRCC 2 cut(s) 701, 887
BsrFI RCCGGY 2 cut(s) 221, 1028
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 3 cut(s) 70, 135, 1193
BssAI RCCGGY 2 cut(s) 221, 1028
BssECI CCNNGG 2 cut(s) 603, 604
BssMI GATC 4 cut(s) 167, 282, 763, 873
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 1 cut(s) 364
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 4 cut(s) 243, 492, 605, 871
Bst4CI ACNGT 3 cut(s) 47, 1077, 1114
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 475
BstBI TTCGAA 2 cut(s) 597, 976
BstC8I GCNNGC 4 cut(s) 223, 401, 1030, 1034
BstDEI CTNAG 2 cut(s) 249, 1070
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 3 cut(s) 107, 488, 906
BstFNI CGCG 2 cut(s) 144, 1126
BstHHI GCGC 1 cut(s) 1126
BstKTI GATC 4 cut(s) 170, 285, 766, 876
BstMBI GATC 4 cut(s) 167, 282, 763, 873
BstMWI GCNNNNNNNGC 4 cut(s) 123, 126, 259, 1104
BstNI CCWGG 4 cut(s) 243, 492, 605, 871
BstNSI RCATGY 3 cut(s) 352, 403, 1036
BstSCI CCNGG 5 cut(s) 154, 241, 490, 603, 869
BstSFI CTRYAG 1 cut(s) 175
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 2 cut(s) 144, 1126
BstV1I GCAGC 7 cut(s) 104, 113, 135, 647, 944, 1110, 1140
BsuRI GGCC 3 cut(s) 24, 268, 649
BtgZI GCGATG 1 cut(s) 752
BtsCI GGATG 3 cut(s) 107, 488, 906
BtsI GCAGTG 1 cut(s) 1108
BtsIMutI CAGTG 4 cut(s) 405, 829, 1108, 1186
Cac8I GCNNGC 4 cut(s) 223, 401, 1030, 1034
CaiI CAGNNNCTG 1 cut(s) 1107
CfoI GCGC 1 cut(s) 1126
Cfr10I RCCGGY 2 cut(s) 221, 1028
Cfr13I GGNCC 2 cut(s) 245, 648
CseI GACGC 3 cut(s) 133, 347, 1137
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 4 cut(s) 138, 185, 476, 969
CviQI GTAC 4 cut(s) 138, 185, 476, 969
DdeI CTNAG 2 cut(s) 249, 1070
DpnI GATC 4 cut(s) 169, 284, 765, 875
DpnII GATC 4 cut(s) 167, 282, 763, 873
DrdI GACNNNNNNGTC 1 cut(s) 1145
DseDI GACNNNNNNGTC 1 cut(s) 1145
EciI GGCGGA 1 cut(s) 1062
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 4 cut(s) 241, 490, 603, 869
EcoRV GATATC 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 321
FaqI GGGAC 3 cut(s) 182, 570, 1172
FauI CCCGC 3 cut(s) 149, 281, 1149
FauNDI CATATG 1 cut(s) 940
FblI GTMKAC 2 cut(s) 42, 1182
Fnu4HI GCNGC 8 cut(s) 118, 121, 124, 127, 636, 933, 1099, 1154
FokI GGATG 3 cut(s) 94, 495, 893
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 8 cut(s) 118, 121, 124, 127, 636, 933, 1099, 1154
FspBI CTAG 3 cut(s) 35, 1119, 1145
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 8 cut(s) 118, 121, 124, 127, 636, 933, 1099, 1154
HaeIII GGCC 3 cut(s) 24, 268, 649
HapII CCGG 3 cut(s) 155, 222, 1029
HgaI GACGC 3 cut(s) 133, 347, 1137
HhaI GCGC 1 cut(s) 1126
Hin1I GRCGYC 1 cut(s) 339
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HincII GTYRAC 2 cut(s) 43, 1183
HindII GTYRAC 2 cut(s) 43, 1183
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 85, 389, 433, 497, 686
HpaII CCGG 3 cut(s) 155, 222, 1029
HphI GGTGA 5 cut(s) 231, 292, 413, 506, 563
Hpy166II GTNNAC 5 cut(s) 43, 205, 262, 530, 1183
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 3 cut(s) 364, 383, 710
Hpy8I GTNNAC 5 cut(s) 43, 205, 262, 530, 1183
Hpy99I CGWCG 2 cut(s) 149, 1131
HpyAV CCTTC 2 cut(s) 87, 268
HpyCH4III ACNGT 3 cut(s) 47, 1077, 1114
HpyCH4IV ACGT 2 cut(s) 258, 845
HpyCH4V TGCA 6 cut(s) 262, 330, 399, 635, 677, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 126, 259, 1104
HpyF3I CTNAG 2 cut(s) 249, 1070
HpySE526I ACGT 2 cut(s) 258, 845
Hsp92I GRCGYC 1 cut(s) 339
HspAI GCGC 1 cut(s) 1124
KroI GCCGGC 2 cut(s) 221, 1028
KroNI GCCGGC 2 cut(s) 223, 1030
Kzo9I GATC 4 cut(s) 167, 282, 763, 873
LmnI GCTCC 1 cut(s) 945
Lsp1109I GCAGC 7 cut(s) 104, 113, 135, 647, 944, 1110, 1140
LweI GCATC 2 cut(s) 339, 473
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 3 cut(s) 35, 1119, 1145
MaeII ACGT 2 cut(s) 258, 845
MaeIII GTNAC 2 cut(s) 494, 1015
MalI GATC 4 cut(s) 169, 284, 765, 875
MboI GATC 4 cut(s) 167, 282, 763, 873
MboII GAAGA 4 cut(s) 283, 398, 422, 425
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 2 cut(s) 264, 321
MluCI AATT 4 cut(s) 13, 639, 678, 955
MlyI GAGTC 2 cut(s) 94, 491
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 9 cut(s) 14, 160, 258, 303, 371, 754, 783, 1129, 1185
MroNI GCCGGC 2 cut(s) 221, 1028
MslI CAYNNNNRTG 2 cut(s) 398, 455
MspA1I CMGCKG 2 cut(s) 117, 126
MspI CCGG 3 cut(s) 155, 222, 1029
MspR9I CCNGG 5 cut(s) 156, 243, 492, 605, 871
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 4 cut(s) 243, 492, 605, 871
MvnI CGCG 2 cut(s) 144, 1126
MwoI GCNNNNNNNGC 4 cut(s) 123, 126, 259, 1104
NaeI GCCGGC 2 cut(s) 223, 1030
NciI CCSGG 1 cut(s) 156
NdeI CATATG 1 cut(s) 940
NdeII GATC 4 cut(s) 167, 282, 763, 873
NgoMIV GCCGGC 2 cut(s) 221, 1028
NlaIV GGNNCC 2 cut(s) 703, 889
NmuCI GTSAC 1 cut(s) 494
NspI RCATGY 3 cut(s) 352, 403, 1036
NspV TTCGAA 2 cut(s) 597, 976
PaeI GCATGC 2 cut(s) 403, 1036
PaeR7I CTCGAG 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 2 cut(s) 223, 1030
PfeI GAWTC 3 cut(s) 389, 433, 686
PfoI TCCNGGA 1 cut(s) 869
PkrI GCNGC 8 cut(s) 119, 122, 125, 128, 637, 934, 1100, 1155
PleI GAGTC 2 cut(s) 93, 491
PpsI GAGTC 2 cut(s) 93, 491
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 4 cut(s) 241, 490, 603, 869
PspGI CCWGG 4 cut(s) 241, 490, 603, 869
PspN4I GGNNCC 2 cut(s) 703, 889
PspPI GGNCC 2 cut(s) 245, 648
PspPPI RGGWCCY 1 cut(s) 245
PspXI VCTCGAGB 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 1107
PvuII CAGCTG 1 cut(s) 126
RsaI GTAC 4 cut(s) 139, 186, 477, 970
RsaNI GTAC 4 cut(s) 138, 185, 476, 969
RseI CAYNNNNRTG 2 cut(s) 398, 455
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 2 cut(s) 41, 1181
SatI GCNGC 8 cut(s) 118, 121, 124, 127, 636, 933, 1099, 1154
Sau3AI GATC 4 cut(s) 167, 282, 763, 873
Sau96I GGNCC 2 cut(s) 245, 648
SchI GAGTC 2 cut(s) 94, 491
ScrFI CCNGG 5 cut(s) 156, 243, 492, 605, 871
SduI GDGCHC 2 cut(s) 264, 321
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 2 cut(s) 339, 473
SfcI CTRYAG 1 cut(s) 175
Sfr274I CTCGAG 1 cut(s) 307
SfuI TTCGAA 2 cut(s) 597, 976
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 2 cut(s) 398, 455
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 2 cut(s) 403, 1036
Sse9I AATT 4 cut(s) 13, 639, 678, 955
SsiI CCGC 9 cut(s) 115, 120, 142, 278, 288, 352, 851, 1047, 1156
SspMI CTAG 3 cut(s) 35, 1119, 1145
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 5 cut(s) 154, 241, 490, 603, 869
TaaI ACNGT 3 cut(s) 47, 1077, 1114
TaiI ACGT 2 cut(s) 261, 848
TaqI TCGA 7 cut(s) 42, 308, 597, 918, 976, 1088, 1182
TasI AATT 4 cut(s) 13, 639, 678, 955
TatI WGTACW 1 cut(s) 475
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 3 cut(s) 389, 433, 686
TscAI CASTG 4 cut(s) 412, 829, 1108, 1193
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 7 cut(s) 117, 123, 126, 635, 932, 1098, 1153
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 5 cut(s) 570, 588, 662, 749, 895
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 4 cut(s) 412, 829, 1108, 1193
VneI GTGCAC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 777
XceI RCATGY 3 cut(s) 352, 403, 1036
XcmI CCANNNNNNNNNTGG 2 cut(s) 783, 979
XhoI CTCGAG 1 cut(s) 307
XmiI GTMKAC 2 cut(s) 42, 1182
XspI CTAG 3 cut(s) 35, 1119, 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.