Rh1CG177200

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000588
Physical Location & Seq
Reverse (-)
39122824 .. 39125634
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG177200.1

Sequence Viewer

Length: 1116 bp
ATGGTGATGAGCAATAACGGTGGCCAACGACAACAAATGGGCTACTGTAGCAATTGCCAGACCCAGATCCCGCTGCCTTCTGAGGTCAACTCTCCGGGCTCCACCCGCTCCCCGCGTAGTGCTGTAGTCTACAATGTCTTTTTAGGTTGCATTTGCACTACTGTTTCTGATAATCAAGTTATTGGAACCGCTGGGGACCATAATGGAAGTTTTTATGTTGGCAACACTCTTCTTCCCTCGCCCTACAACCACGCGCCGCCGGAACGTCAGCCGAAAGTGCACAGGCCGAAGAAGGCGGTGATCTGCGGAATATCGTATAAAAAGTCGCCGCATGAGCTCAAGGGTTGCATCAATGATGCCAAATGCATGCGTAATCTCCTCATCAACGAGTTCGAGTTTCCGGAAGATTCCATTACCATGCTCACTGAAGAAAAAACTGACCCATGTAAGATTCCCTATAAAAACAACATTGAAAGGGAATTACGTTGGCTTGTACGAGGATGTCAACATGGTGACTCCCTTCTGTTTTACTTCTCTGGTCATGTTTCAAGGCTGAGGAATCTGTATCCTCATGATGAAGAAGATAGATATCATGAAACCCTCTGTCCCCTTGAGTTCGAAACCCAGGGTATGATTGTTGATGATTGGATAAATGCAACAATTGTTAGGCCAATTCCAGATGGGGTCAAGCTTCATGCAATTATAGATTGTTCTCATAGTGGCACCATACTGGATTTGCCATGGCTTTGCAGAATGGACGGGGGTGGAAACTATACATGGGAAAAGGCATCAGGCATATGGAAAGGAACAAGTGGTGGAGAAGTTATATGTATCAGTGGCTGTGTTGACCATCAAACCTCTACGGAAACACAATTAATCACACCAACAGGTACCATGACTTCCTGCTTCATCCAAGCAATCGAGAGTGGACAGGCAGCCACATATGGGAGCTTACTCGATCTTATGCGTAATAACATTCGAAGTAGGGATGCTGTTACATATCTTGCCGGCATGCTTCAAACAGGCGGAAGTGTTGGTGGTGGTAGGCTAAGACAGGATCCTCAATTAAGTGCATGCAAGCAGTTCCATGTGTACCAGAAACCTTTTGCCCTGTAA

Protein Analysis

371

Amino Acids

41.13

Weight (kDa)

6.45

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 97 - 358 3.9e-27 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 890
AccB1I GGYRCC 2 cut(s) 722, 890
AccBSI CCGCTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 129
AccII CGCG 2 cut(s) 115, 254
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 9 cut(s) 71, 106, 113, 189, 257, 296, 306, 329, 1026
AclWI GGATC 3 cut(s) 61, 1052, 1065
AcoI YGGCCR 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 3 cut(s) 495, 892, 1094
AfiI CCNNNNNNNGG 1 cut(s) 945
AgsI TTSAA 3 cut(s) 473, 549, 1019
AjnI CCWGG 1 cut(s) 624
AluBI AGCT 3 cut(s) 337, 691, 951
AluI AGCT 3 cut(s) 337, 691, 951
Alw21I GWGCWC 2 cut(s) 282, 339
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 3 cut(s) 61, 1052, 1065
AlwNI CAGNNNCTG 1 cut(s) 840
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 22, 284, 668
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 2 cut(s) 73, 935
AseI ATTAAT 1 cut(s) 875
Asp718I GGTACC 1 cut(s) 890
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 16, 310, 524
AsuII TTCGAA 2 cut(s) 618, 979
AvaII GGWCC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 282
BalI TGGCCA 1 cut(s) 24
BamHI GGATCC 1 cut(s) 1057
BanI GGYRCC 2 cut(s) 722, 890
BanII GRGCYC 2 cut(s) 101, 339
BarI GAAGNNNNNNTAC 2 cut(s) 883, 915
Bbv12I GWGCWC 2 cut(s) 282, 339
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 60, 947
BccI CCATC 2 cut(s) 674, 858
BciT130I CCWGG 1 cut(s) 626
BciVI GTATCC 1 cut(s) 576
BcnI CCSGG 1 cut(s) 96
BfmI CTRYAG 2 cut(s) 46, 123
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 4 cut(s) 74, 257, 329, 936
BlsI GCNGC 4 cut(s) 75, 258, 330, 937
Bme1390I CCNGG 2 cut(s) 96, 626
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 6 cut(s) 100, 187, 197, 724, 892, 1059
BmrFI CCNGG 2 cut(s) 96, 626
BmsI GCATC 4 cut(s) 346, 357, 797, 979
BplI GAGNNNNNCTC 2 cut(s) 74, 106
Bpu10I CCTNAGC 1 cut(s) 554
Bpu14I TTCGAA 2 cut(s) 618, 979
BpuEI CTTGAG 2 cut(s) 323, 632
BpuMI CCSGG 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 3 cut(s) 624, 625, 740
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 1 cut(s) 945
Bse118I RCCGGY 1 cut(s) 1007
Bse1I ACTGG 1 cut(s) 735
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 3 cut(s) 624, 625, 740
BseGI GGATG 3 cut(s) 506, 909, 994
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 1 cut(s) 945
BseMII CTCAG 2 cut(s) 72, 545
BseNI ACTGG 1 cut(s) 735
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 2 cut(s) 60, 947
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 115, 254
BshFI GGCC 3 cut(s) 24, 286, 670
BshNI GGYRCC 2 cut(s) 722, 890
BsiHKAI GWGCWC 2 cut(s) 282, 339
BsiSI CCGG 4 cut(s) 95, 260, 401, 1008
BslFI GGGAC 2 cut(s) 209, 591
BslI CCNNNNNNNGG 1 cut(s) 945
BsmFI GGGAC 2 cut(s) 209, 591
BsnI GGCC 3 cut(s) 24, 286, 670
Bsp119I TTCGAA 2 cut(s) 618, 979
Bsp1286I GDGCHC 3 cut(s) 101, 282, 339
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 4 cut(s) 66, 300, 958, 1057
Bsp19I CCATGG 1 cut(s) 740
BspACI CCGC 9 cut(s) 71, 106, 113, 189, 257, 296, 306, 329, 1026
BspANI GGCC 3 cut(s) 24, 286, 670
BspCNI CTCAG 2 cut(s) 73, 546
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 2 cut(s) 115, 254
BspHI TCATGA 2 cut(s) 571, 592
BspLI GGNNCC 6 cut(s) 100, 187, 197, 724, 892, 1059
BspPI GGATC 3 cut(s) 61, 1052, 1065
BspT104I TTCGAA 2 cut(s) 618, 979
BspT107I GGYRCC 2 cut(s) 722, 890
BsrBI CCGCTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 1007
BsrI ACTGG 1 cut(s) 735
BssAI RCCGGY 1 cut(s) 1007
BssECI CCNNGG 3 cut(s) 624, 625, 740
BssMI GATC 4 cut(s) 66, 300, 958, 1057
BssT1I CCWWGG 1 cut(s) 740
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 3 cut(s) 20, 47, 163
Bst6I CTCTTC 1 cut(s) 234
BstBI TTCGAA 2 cut(s) 618, 979
BstC8I GCNNGC 5 cut(s) 368, 1009, 1013, 1075, 1079
BstDEI CTNAG 3 cut(s) 81, 554, 1049
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 3 cut(s) 506, 909, 994
BstFNI CGCG 2 cut(s) 115, 254
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 69, 303, 961, 1060
BstMBI GATC 4 cut(s) 66, 300, 958, 1057
BstMWI GCNNNNNNNGC 4 cut(s) 48, 105, 277, 334
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 3 cut(s) 370, 1015, 1077
BstSCI CCNGG 2 cut(s) 94, 624
BstSFI CTRYAG 2 cut(s) 46, 123
BstSLI GKGCMC 1 cut(s) 282
BstUI CGCG 2 cut(s) 115, 254
BstV1I GCAGC 2 cut(s) 60, 947
BstX2I RGATCY 2 cut(s) 66, 1057
BstYI RGATCY 2 cut(s) 66, 1057
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 24, 286, 670
BtgI CCRYGG 1 cut(s) 740
BtsCI GGATG 3 cut(s) 506, 909, 994
BtsIMutI CAGTG 2 cut(s) 423, 841
Cac8I GCNNGC 5 cut(s) 368, 1009, 1013, 1075, 1079
CaiI CAGNNNCTG 1 cut(s) 840
CciI TCATGA 2 cut(s) 571, 592
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 1007
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 3 cut(s) 494, 891, 1093
CspCI CAANNNNNGTGG 1 cut(s) 36
CviQI GTAC 3 cut(s) 494, 891, 1093
DdeI CTNAG 3 cut(s) 81, 554, 1049
DpnI GATC 4 cut(s) 68, 302, 960, 1059
DpnII GATC 4 cut(s) 66, 300, 958, 1057
EaeI YGGCCR 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 1041
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 740
Eco24I GRGCYC 2 cut(s) 101, 339
Eco32I GATATC 1 cut(s) 590
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 624
EcoRV GATATC 1 cut(s) 590
EcoT14I CCWWGG 1 cut(s) 740
EcoT22I ATGCAT 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 101, 339
ErhI CCWWGG 1 cut(s) 740
FaqI GGGAC 2 cut(s) 209, 591
FauI CCCGC 3 cut(s) 78, 113, 120
FauNDI CATATG 2 cut(s) 797, 943
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 74, 257, 329, 936
FokI GGATG 3 cut(s) 513, 896, 1001
FriOI GRGCYC 2 cut(s) 101, 339
Fsp4HI GCNGC 4 cut(s) 74, 257, 329, 936
GlaI GCGC 1 cut(s) 255
GluI GCNGC 4 cut(s) 74, 257, 329, 936
GsaI CCCAGC 1 cut(s) 195
HaeIII GGCC 3 cut(s) 24, 286, 670
HapII CCGG 4 cut(s) 95, 260, 401, 1008
HhaI GCGC 1 cut(s) 256
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 3 cut(s) 88, 506, 847
HindII GTYRAC 3 cut(s) 88, 506, 847
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 407, 451, 515, 559
HpaII CCGG 4 cut(s) 95, 260, 401, 1008
HphI GGTGA 3 cut(s) 16, 310, 524
Hpy166II GTNNAC 7 cut(s) 88, 130, 280, 506, 847, 929, 1093
Hpy188I TCNGA 2 cut(s) 82, 169
Hpy188III TCNNGA 5 cut(s) 401, 572, 593, 677, 922
Hpy8I GTNNAC 7 cut(s) 88, 130, 280, 506, 847, 929, 1093
HpyAV CCTTC 3 cut(s) 87, 286, 530
HpyCH4III ACNGT 3 cut(s) 20, 47, 163
HpyCH4IV ACGT 2 cut(s) 265, 484
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 105, 277, 334
HpyF3I CTNAG 3 cut(s) 81, 554, 1049
HpySE526I ACGT 2 cut(s) 265, 484
HspAI GCGC 1 cut(s) 254
Kpn2I TCCGGA 1 cut(s) 400
KpnI GGTACC 1 cut(s) 894
KroI GCCGGC 1 cut(s) 1007
KroNI GCCGGC 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 66, 300, 958, 1057
LmnI GCTCC 3 cut(s) 104, 113, 948
Lsp1109I GCAGC 2 cut(s) 60, 947
LweI GCATC 4 cut(s) 346, 357, 797, 979
MaeII ACGT 2 cut(s) 265, 484
MaeIII GTNAC 2 cut(s) 512, 994
MalI GATC 4 cut(s) 68, 302, 960, 1059
MbiI CCGCTC 1 cut(s) 108
MboI GATC 4 cut(s) 66, 300, 958, 1057
MboII GAAGA 7 cut(s) 221, 224, 301, 416, 440, 590, 593
MfeI CAATTG 2 cut(s) 52, 660
MflI RGATCY 2 cut(s) 66, 1057
MhlI GDGCHC 3 cut(s) 101, 282, 339
MlsI TGGCCA 1 cut(s) 24
MluCI AATT 7 cut(s) 52, 479, 660, 672, 699, 872, 1064
MluNI TGGCCA 1 cut(s) 24
MlyI GAGTC 1 cut(s) 509
MnlI CCTC 9 cut(s) 76, 247, 389, 491, 549, 579, 611, 868, 1071
Mox20I TGGCCA 1 cut(s) 24
Mph1103I ATGCAT 1 cut(s) 368
MroI TCCGGA 1 cut(s) 400
MroNI GCCGGC 1 cut(s) 1007
MscI TGGCCA 1 cut(s) 24
MseI TTAA 2 cut(s) 875, 1067
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 24
MspA1I CMGCKG 2 cut(s) 73, 191
MspI CCGG 4 cut(s) 95, 260, 401, 1008
MspR9I CCNGG 2 cut(s) 96, 626
MunI CAATTG 2 cut(s) 52, 660
MvaI CCWGG 1 cut(s) 626
MvnI CGCG 2 cut(s) 115, 254
MwoI GCNNNNNNNGC 4 cut(s) 48, 105, 277, 334
NaeI GCCGGC 1 cut(s) 1009
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 1 cut(s) 740
NdeI CATATG 2 cut(s) 797, 943
NdeII GATC 4 cut(s) 66, 300, 958, 1057
NgoMIV GCCGGC 1 cut(s) 1007
NlaIV GGNNCC 6 cut(s) 100, 187, 197, 724, 892, 1059
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 368
NspI RCATGY 3 cut(s) 370, 1015, 1077
NspV TTCGAA 2 cut(s) 618, 979
PaeI GCATGC 3 cut(s) 370, 1015, 1077
PagI TCATGA 2 cut(s) 571, 592
PasI CCCWGGG 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 1009
PfeI GAWTC 3 cut(s) 407, 451, 559
PkrI GCNGC 4 cut(s) 75, 258, 330, 937
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PshBI ATTAAT 1 cut(s) 875
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 624
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 6 cut(s) 100, 187, 197, 724, 892, 1059
PspPI GGNCC 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 840
PsuI RGATCY 2 cut(s) 66, 1057
RsaI GTAC 3 cut(s) 495, 892, 1094
RsaNI GTAC 3 cut(s) 494, 891, 1093
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 875, 1067
SatI GCNGC 4 cut(s) 74, 257, 329, 936
Sau3AI GATC 4 cut(s) 66, 300, 958, 1057
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 2 cut(s) 96, 626
SduI GDGCHC 3 cut(s) 101, 282, 339
SfaNI GCATC 4 cut(s) 346, 357, 797, 979
SfcI CTRYAG 2 cut(s) 46, 123
SfuI TTCGAA 2 cut(s) 618, 979
SinI GGWCC 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 338, 611
SmoI CTYRAG 2 cut(s) 338, 611
SphI GCATGC 3 cut(s) 370, 1015, 1077
Sse9I AATT 7 cut(s) 52, 479, 660, 672, 699, 872, 1064
SsiI CCGC 9 cut(s) 71, 106, 113, 189, 257, 296, 306, 329, 1026
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 2 cut(s) 94, 624
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 3 cut(s) 20, 47, 163
TaiI ACGT 2 cut(s) 268, 487
TaqI TCGA 5 cut(s) 393, 618, 921, 957, 979
TasI AATT 7 cut(s) 52, 479, 660, 672, 699, 872, 1064
TauI GCSGC 2 cut(s) 259, 331
TfiI GAWTC 3 cut(s) 407, 451, 559
Tru1I TTAA 2 cut(s) 875, 1067
Tru9I TTAA 2 cut(s) 875, 1067
TscAI CASTG 2 cut(s) 430, 841
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 73, 935
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 4 cut(s) 591, 609, 683, 898
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 2 cut(s) 430, 841
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 196
VspI ATTAAT 1 cut(s) 875
XceI RCATGY 3 cut(s) 370, 1015, 1077
XmiI GTMKAC 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.