Rmu_sc0007317.1_g000002

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007317.1
OG0000588
Physical Location & Seq
Reverse (-)
11149 .. 14668
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007317.1_g000002.1.cds

Sequence Viewer

Length: 1314 bp
atgatgatgagcaattatggaggccaacgacttctagtggtcgactgttgctattgccagacccaaatccagctgccttctgtgagtccgatatacatcgtcggcacatccgtccgctgcggccgctgctatgccagtacccgcgtcgcctctccgggcttcccccgctccccccatagtgccgtaccctactatgcccttataaacccaccaccacatgcctgcttcccccatacatcaccaggacctccgccgaacgtgcatgggcggaagaaggcggtgatctgcgggatatcgtatagaaactcgagggatgagctcaattgttgcatcaatgacgccaaatgcatgcggtatctcctcatcaccaagttcaagtttccagaagattccattctcatgctcactgaagaagaaactcacccacatatgattccatataaaaacaacattagaagggcattatgttggcttgtacaaggatgtcaatcgggggactcccttctgttttacttctccggtcatggttcacggcagaggaattatgatagtgatgaagttgatggatatgatgaaaccctttgcccccttgactttgaaacccagggtttgattgttgatgatgagataaatgcagcaattgttaggcccattccacatggggtcaagcttcatgcaattatagattcttgtcatagtggcaccatactggatttgccatgcctttgcagaatggacaggggtggaaaatatgtatgggaggatcatcgacctccatcaggcgtatggaaaggaacaagtggtggacaagttatatctatcagcggttgtgatgaccatcaaacctctgcagagacatcagcagccacatatgggagcttactcatatctatgcgtaataccattcgaggtatgggttctggtggtggtggtagtggtggtgctgttacatctcttgccggcatgcttctaacaggcggaagtgttggtggtggtaggctaagacaggatcatcgacctccatcaggcgtatggaaaggaacaagtggtggacaagttatatctatcagcggttgtgatgaccatcaaacctctgcagagacatcagcagccacatatgggagcttactcatatctatgcgtaataccattcgaggtatgggttctggtggtggtggtagtggtggtgctgttacatctcttgccggcatgcttctaacaggcggaagtgttggtggtggtaggctaagacagaagcctcaattaactggctgcgagccgttcgatgtgtacacgaaaccttttgccctatga

Protein Analysis

437

Amino Acids

46.57

Weight (kDa)

8.05

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 701
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 42
AccII CGCG 1 cut(s) 144
AclWI GGATC 2 cut(s) 771, 1017
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 4 cut(s) 139, 186, 477, 1292
AfiI CCNNNNNNNGG 4 cut(s) 779, 873, 1025, 1119
AgsI TTSAA 2 cut(s) 376, 599
AjnI CCWGG 2 cut(s) 241, 603
AluBI AGCT 5 cut(s) 73, 319, 670, 879, 1125
AluI AGCT 5 cut(s) 73, 319, 670, 879, 1125
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 848, 1094
AlwI GGATC 2 cut(s) 771, 1017
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 3 cut(s) 22, 121, 647
ApeKI GCWGC 7 cut(s) 73, 117, 126, 635, 863, 1109, 1272
AspS9I GGNCC 2 cut(s) 245, 648
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 4 cut(s) 231, 292, 358, 413
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 245
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 7 cut(s) 60, 104, 113, 647, 875, 1121, 1259
BccI CCATC 5 cut(s) 557, 784, 846, 1030, 1092
BceAI ACGGC 3 cut(s) 167, 548, 1264
BciT130I CCWGG 2 cut(s) 243, 605
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 848, 1094
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 2 cut(s) 849, 1095
BisI GCNGC 9 cut(s) 74, 118, 121, 124, 127, 636, 864, 1110, 1273
BlsI GCNGC 9 cut(s) 75, 119, 122, 125, 128, 637, 865, 1111, 1274
Bme1390I CCNGG 3 cut(s) 156, 243, 605
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 245, 648
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 3 cut(s) 156, 243, 605
BmsI GCATC 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 156
BsaBI GATNNNNATC 4 cut(s) 95, 487, 837, 1083
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 603, 604
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 4 cut(s) 779, 873, 1025, 1119
Bse118I RCCGGY 2 cut(s) 959, 1205
Bse1I ACTGG 3 cut(s) 135, 714, 1273
Bse8I GATNNNNATC 4 cut(s) 95, 487, 837, 1083
BseBI CCWGG 2 cut(s) 243, 605
BseDI CCNNGG 2 cut(s) 603, 604
BseGI GGATG 3 cut(s) 107, 319, 488
BseJI GATNNNNATC 4 cut(s) 95, 487, 837, 1083
BseLI CCNNNNNNNGG 4 cut(s) 779, 873, 1025, 1119
BseNI ACTGG 3 cut(s) 135, 714, 1273
BseRI GAGGAG 1 cut(s) 350
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 7 cut(s) 60, 104, 113, 647, 875, 1121, 1259
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 24, 123, 649
BshNI GGYRCC 1 cut(s) 701
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 4 cut(s) 155, 519, 960, 1206
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 4 cut(s) 779, 873, 1025, 1119
BsmAI GTCTC 2 cut(s) 848, 1094
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 3 cut(s) 24, 123, 649
BsoBI CYCGRG 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1407I TGTACA 2 cut(s) 475, 1290
Bsp143I GATC 3 cut(s) 282, 763, 1009
BspANI GGCC 3 cut(s) 24, 123, 649
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 703
BspMAI CTGCAG 2 cut(s) 853, 1099
BspPI GGATC 2 cut(s) 771, 1017
BspT107I GGYRCC 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 168
BsrFI RCCGGY 2 cut(s) 959, 1205
BsrGI TGTACA 2 cut(s) 475, 1290
BsrI ACTGG 3 cut(s) 135, 714, 1273
BssAI RCCGGY 2 cut(s) 959, 1205
BssECI CCNNGG 2 cut(s) 603, 604
BssMI GATC 3 cut(s) 282, 763, 1009
BssNI GRCGYC 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 243, 605
Bst4CI ACNGT 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 2 cut(s) 475, 1290
BstC8I GCNNGC 7 cut(s) 223, 350, 961, 965, 1207, 1211, 1277
BstDEI CTNAG 2 cut(s) 1001, 1247
BstENI CCTNNNNNAGG 2 cut(s) 777, 1023
BstF5I GGATG 3 cut(s) 107, 319, 488
BstFNI CGCG 1 cut(s) 144
BstKTI GATC 3 cut(s) 285, 766, 1012
BstMAI GTCTC 2 cut(s) 848, 1094
BstMBI GATC 3 cut(s) 282, 763, 1009
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
BstNI CCWGG 2 cut(s) 243, 605
BstNSI RCATGY 4 cut(s) 221, 352, 967, 1213
BstSCI CCNGG 3 cut(s) 154, 241, 603
BstSFI CTRYAG 2 cut(s) 849, 1095
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 7 cut(s) 60, 104, 113, 647, 875, 1121, 1259
BstZI CGGCCG 1 cut(s) 121
BsuRI GGCC 3 cut(s) 24, 123, 649
BtsCI GGATG 3 cut(s) 107, 319, 488
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 7 cut(s) 223, 350, 961, 965, 1207, 1211, 1277
CciNI GCGGCCGC 1 cut(s) 121
Cfr10I RCCGGY 2 cut(s) 959, 1205
Cfr13I GGNCC 2 cut(s) 245, 648
CseI GACGC 2 cut(s) 133, 347
Csp6I GTAC 4 cut(s) 138, 185, 476, 1291
CviQI GTAC 4 cut(s) 138, 185, 476, 1291
DdeI CTNAG 2 cut(s) 1001, 1247
DpnI GATC 3 cut(s) 284, 765, 1011
DpnII GATC 3 cut(s) 282, 763, 1009
EaeI YGGCCR 1 cut(s) 121
EagI CGGCCG 1 cut(s) 121
EciI GGCGGA 4 cut(s) 240, 283, 993, 1239
Ecl136II GAGCTC 1 cut(s) 319
EclXI CGGCCG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 2 cut(s) 777, 1023
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 241, 603
EcoRV GATATC 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 321
FaqI GGGAC 1 cut(s) 509
FauI CCCGC 3 cut(s) 149, 173, 281
FauNDI CATATG 3 cut(s) 429, 871, 1117
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 9 cut(s) 74, 118, 121, 124, 127, 636, 864, 1110, 1273
FokI GGATG 3 cut(s) 94, 326, 495
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 9 cut(s) 74, 118, 121, 124, 127, 636, 864, 1110, 1273
FspBI CTAG 1 cut(s) 35
GluI GCNGC 9 cut(s) 74, 118, 121, 124, 127, 636, 864, 1110, 1273
HaeIII GGCC 3 cut(s) 24, 123, 649
HapII CCGG 4 cut(s) 155, 519, 960, 1206
HgaI GACGC 2 cut(s) 133, 347
Hin1I GRCGYC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 85, 389, 433, 497, 686
HpaII CCGG 4 cut(s) 155, 519, 960, 1206
HphI GGTGA 4 cut(s) 231, 292, 358, 413
Hpy166II GTNNAC 6 cut(s) 43, 530, 806, 1052, 1291, 1293
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 6 cut(s) 43, 530, 806, 1052, 1291, 1293
Hpy99I CGWCG 2 cut(s) 104, 149
HpyAV CCTTC 4 cut(s) 87, 268, 450, 512
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 8 cut(s) 262, 330, 348, 635, 677, 729, 851, 1097
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
HpyF3I CTNAG 2 cut(s) 1001, 1247
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 339
KroI GCCGGC 2 cut(s) 959, 1205
KroNI GCCGGC 2 cut(s) 961, 1207
Kzo9I GATC 3 cut(s) 282, 763, 1009
LmnI GCTCC 3 cut(s) 173, 876, 1122
Lsp1109I GCAGC 7 cut(s) 60, 104, 113, 647, 875, 1121, 1259
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 35
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 946, 1192
MalI GATC 3 cut(s) 284, 765, 1011
MbiI CCGCTC 1 cut(s) 168
MboI GATC 3 cut(s) 282, 763, 1009
MboII GAAGA 4 cut(s) 283, 398, 422, 425
MfeI CAATTG 2 cut(s) 322, 639
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 6 cut(s) 13, 322, 541, 639, 678, 1262
MlyI GAGTC 2 cut(s) 94, 491
Mph1103I ATGCAT 1 cut(s) 350
MroNI GCCGGC 2 cut(s) 959, 1205
MseI TTAA 1 cut(s) 1265
MslI CAYNNNNRTG 3 cut(s) 398, 890, 1136
MspA1I CMGCKG 5 cut(s) 73, 117, 126, 825, 1071
MspI CCGG 4 cut(s) 155, 519, 960, 1206
MspR9I CCNGG 3 cut(s) 156, 243, 605
MunI CAATTG 2 cut(s) 322, 639
MvaI CCWGG 2 cut(s) 243, 605
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
NaeI GCCGGC 2 cut(s) 961, 1207
NciI CCSGG 1 cut(s) 156
NdeI CATATG 3 cut(s) 429, 871, 1117
NdeII GATC 3 cut(s) 282, 763, 1009
NgoMIV GCCGGC 2 cut(s) 959, 1205
NlaIV GGNNCC 1 cut(s) 703
NotI GCGGCCGC 1 cut(s) 121
NsiI ATGCAT 1 cut(s) 350
NspI RCATGY 4 cut(s) 221, 352, 967, 1213
PaeI GCATGC 3 cut(s) 352, 967, 1213
PaeR7I CTCGAG 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 2 cut(s) 961, 1207
PfeI GAWTC 3 cut(s) 389, 433, 686
PkrI GCNGC 9 cut(s) 75, 119, 122, 125, 128, 637, 865, 1111, 1274
PleI GAGTC 2 cut(s) 93, 491
PpsI GAGTC 2 cut(s) 93, 491
PpuMI RGGWCCY 1 cut(s) 245
PsiI TTATAA 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 241, 603
PspGI CCWGG 2 cut(s) 241, 603
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 2 cut(s) 245, 648
PspPPI RGGWCCY 1 cut(s) 245
PspXI VCTCGAGB 1 cut(s) 307
PstI CTGCAG 2 cut(s) 853, 1099
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 4 cut(s) 139, 186, 477, 1292
RsaNI GTAC 4 cut(s) 138, 185, 476, 1291
RseI CAYNNNNRTG 3 cut(s) 398, 890, 1136
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 1265
SatI GCNGC 9 cut(s) 74, 118, 121, 124, 127, 636, 864, 1110, 1273
Sau3AI GATC 3 cut(s) 282, 763, 1009
Sau96I GGNCC 2 cut(s) 245, 648
SchI GAGTC 2 cut(s) 94, 491
ScrFI CCNGG 3 cut(s) 156, 243, 605
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 2 cut(s) 849, 1095
Sfr274I CTCGAG 1 cut(s) 307
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 3 cut(s) 398, 890, 1136
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SphI GCATGC 3 cut(s) 352, 967, 1213
Sse9I AATT 6 cut(s) 13, 322, 541, 639, 678, 1262
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 154, 241, 603
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 261
TaqI TCGA 7 cut(s) 42, 308, 769, 907, 1015, 1153, 1284
TasI AATT 6 cut(s) 13, 322, 541, 639, 678, 1262
TatI WGTACW 2 cut(s) 475, 1290
TauI GCSGC 2 cut(s) 123, 126
TfiI GAWTC 3 cut(s) 389, 433, 686
Tru1I TTAA 1 cut(s) 1265
Tru9I TTAA 1 cut(s) 1265
TscAI CASTG 1 cut(s) 412
TseI GCWGC 7 cut(s) 73, 117, 126, 635, 863, 1109, 1272
TspDTI ATGAA 3 cut(s) 570, 588, 662
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 2 cut(s) 777, 1023
XceI RCATGY 4 cut(s) 221, 352, 967, 1213
XcmI CCANNNNNNNNNTGG 4 cut(s) 783, 910, 1029, 1156
XhoI CTCGAG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.