RchiOBHm_Chr1g0344661

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000588
Physical Location & Seq
Reverse (-)
37014050 .. 37017116
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57107

Sequence Viewer

Length: 1104 bp
ATGGGGTACTGTTGCAATTGCCAGACCCAGATCCCGCTGCCTTCTGAGGCCAACTCTCCGGGCTCCACCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCATAGTGTTGTAACCTACAATGTCTATGTAGGTTGCAGTTGCACTAATGTTTCTGATATTCAAGTTATTGGAAACATTGGGGACCATAATGGAAGTTTTTATGTTCGCAACACTACTCCCCCTCCCTTGCCCTACAACCACGCGCCGCCGGGACTTCCGCCGAACGTGTACGGCCGGAAGAAGGCTGTGATCTGCGGGATATCGTATAAAAAGTCGCCGCATGAGCTCAAGGGTTGCATCAATGATGCCAAATGCATGCGTAATCTCCTCATCAACGAGTTCGAGTTTCCGGAAGACTCCATTATCATGCTCACTGAAGAAAAAACTGACCCATGTAAGATTCCCTATAAAAACAACATTGAAAGGGAATTACGTTGGCTTGTACGAGGATGTCAACATGGTGACTCCCTCCTGTTTTACTTCTCTGGTCATGTTTCAAGGCTGAGGAAGCTGTATCCTCATGATGAAGAAGATAGATATCATGAAACCCTCTGTCCCCTTGAGTTCGAAACCCAGGGTATGATTGTTGATGATTGGATAAATGCAACAATTGTTAGGCCAATTCCAGATGGGGTCAAGCTTCATGCAATTATAGATTGTTCTCATAGTGGCACCATCTTGGATTTGCCATGGCTTTGCAGAATGGACGGGGGTGGAAACTATAGATGGGACAAGGCATCAGGCATAGGGAAAGGAACAAGTGGTGGAGAAGTTATATGTATCAGTGGCTGTGTTGACCATCAAACCTCTACGGAAACACAATCAATCACATCAACAGGTACCATGACTTCCTGCTTCATCCAAGCAATCGAGAGTGGACAGGCAGCCACATATGGGAGCTTACTCGATCTTATGCGTAATAACATTCGAAGTAGGGATGCTGTTACATATCTTGCCGGCATGCTTCAAACAGGCGGAAGTGTTGGTGGTGGTAGGCTAAGACAGGATCCTCAATTAAGTGCATGCAAGCAGTTCCATGTGTACCAGAAACCTTTTGCCCTGTAA

Protein Analysis

367

Amino Acids

40.45

Weight (kDa)

6.45

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 92 - 354 1.7e-28 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 710, 878
AccII CGCG 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 7 cut(s) 35, 70, 245, 257, 294, 317, 1014
AclWI GGATC 3 cut(s) 25, 1040, 1053
AcoI YGGCCR 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 5 cut(s) 8, 269, 483, 880, 1082
AfiI CCNNNNNNNGG 2 cut(s) 280, 933
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 4 cut(s) 161, 461, 537, 1007
AjnI CCWGG 1 cut(s) 612
AluBI AGCT 4 cut(s) 325, 550, 679, 939
AluI AGCT 4 cut(s) 325, 550, 679, 939
Alw21I GWGCWC 1 cut(s) 327
AlwI GGATC 3 cut(s) 25, 1040, 1053
AlwNI CAGNNNCTG 1 cut(s) 828
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 3 cut(s) 48, 271, 656
ApeKI GCWGC 2 cut(s) 37, 923
Asp718I GGTACC 1 cut(s) 878
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 2 cut(s) 60, 249
AsuHPI GGTGA 1 cut(s) 512
AsuII TTCGAA 2 cut(s) 606, 967
AvaII GGWCC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 1045
BanI GGYRCC 2 cut(s) 710, 878
BanII GRGCYC 2 cut(s) 65, 327
BarI GAAGNNNNNNTAC 2 cut(s) 871, 903
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 542
BbvI GCAGC 2 cut(s) 24, 935
BccI CCATC 4 cut(s) 662, 722, 759, 846
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 614
BciVI GTATCC 1 cut(s) 564
BcnI CCSGG 2 cut(s) 60, 249
BfmI CTRYAG 1 cut(s) 760
BfuI GTATCC 1 cut(s) 564
BisI GCNGC 4 cut(s) 38, 245, 317, 924
BlsI GCNGC 4 cut(s) 39, 246, 318, 925
Bme1390I CCNGG 3 cut(s) 60, 249, 614
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 5 cut(s) 64, 182, 712, 880, 1047
BmrFI CCNGG 3 cut(s) 60, 249, 614
BmsI GCATC 4 cut(s) 334, 345, 785, 967
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 38, 70
Bpu10I CCTNAGC 1 cut(s) 542
Bpu14I TTCGAA 2 cut(s) 606, 967
BpuEI CTTGAG 2 cut(s) 311, 620
BpuMI CCSGG 2 cut(s) 60, 249
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 3 cut(s) 612, 613, 728
BsaWI WCCGGW 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 933
Bse118I RCCGGY 1 cut(s) 995
Bse8I GATNNNNATC 1 cut(s) 576
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 3 cut(s) 612, 613, 728
BseGI GGATG 3 cut(s) 494, 897, 982
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 280, 933
BseMII CTCAG 2 cut(s) 36, 533
BseRI GAGGAG 1 cut(s) 356
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 2 cut(s) 24, 935
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 3 cut(s) 50, 273, 658
BshNI GGYRCC 2 cut(s) 710, 878
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 5 cut(s) 59, 248, 274, 389, 996
BslFI GGGAC 4 cut(s) 194, 264, 579, 782
BslI CCNNNNNNNGG 2 cut(s) 280, 933
BsmFI GGGAC 4 cut(s) 194, 264, 579, 782
BsnI GGCC 3 cut(s) 50, 273, 658
Bsp119I TTCGAA 2 cut(s) 606, 967
Bsp1286I GDGCHC 2 cut(s) 65, 327
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 4 cut(s) 30, 288, 946, 1045
Bsp19I CCATGG 1 cut(s) 728
BspACI CCGC 7 cut(s) 35, 70, 245, 257, 294, 317, 1014
BspANI GGCC 3 cut(s) 50, 273, 658
BspCNI CTCAG 2 cut(s) 37, 534
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 2 cut(s) 559, 580
BspLI GGNNCC 5 cut(s) 64, 182, 712, 880, 1047
BspPI GGATC 3 cut(s) 25, 1040, 1053
BspT104I TTCGAA 2 cut(s) 606, 967
BspT107I GGYRCC 2 cut(s) 710, 878
BsrFI RCCGGY 1 cut(s) 995
BssAI RCCGGY 1 cut(s) 995
BssECI CCNNGG 3 cut(s) 612, 613, 728
BssMI GATC 4 cut(s) 30, 288, 946, 1045
BssT1I CCWWGG 1 cut(s) 728
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 1 cut(s) 11
BstBI TTCGAA 2 cut(s) 606, 967
BstC8I GCNNGC 5 cut(s) 356, 997, 1001, 1063, 1067
BstDEI CTNAG 3 cut(s) 45, 542, 1037
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 3 cut(s) 494, 897, 982
BstFNI CGCG 1 cut(s) 242
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 4 cut(s) 33, 291, 949, 1048
BstMBI GATC 4 cut(s) 30, 288, 946, 1045
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 3 cut(s) 69, 322, 547
BstNI CCWGG 1 cut(s) 614
BstNSI RCATGY 3 cut(s) 358, 1003, 1065
BstSCI CCNGG 3 cut(s) 58, 247, 612
BstSFI CTRYAG 1 cut(s) 760
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 24, 935
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 2 cut(s) 30, 1045
BstYI RGATCY 2 cut(s) 30, 1045
BstZI CGGCCG 1 cut(s) 271
BsuI GTATCC 1 cut(s) 564
BsuRI GGCC 3 cut(s) 50, 273, 658
BtgI CCRYGG 1 cut(s) 728
BtsCI GGATG 3 cut(s) 494, 897, 982
BtsIMutI CAGTG 2 cut(s) 411, 829
Cac8I GCNNGC 5 cut(s) 356, 997, 1001, 1063, 1067
CaiI CAGNNNCTG 1 cut(s) 828
CciI TCATGA 2 cut(s) 559, 580
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 995
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 5 cut(s) 7, 268, 482, 879, 1081
CviQI GTAC 5 cut(s) 7, 268, 482, 879, 1081
DdeI CTNAG 3 cut(s) 45, 542, 1037
DpnI GATC 4 cut(s) 32, 290, 948, 1047
DpnII GATC 4 cut(s) 30, 288, 946, 1045
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
EciI GGCGGA 2 cut(s) 246, 1029
Ecl136II GAGCTC 1 cut(s) 325
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 728
Eco24I GRGCYC 2 cut(s) 65, 327
Eco32I GATATC 2 cut(s) 300, 578
Eco47I GGWCC 1 cut(s) 181
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 612
EcoRV GATATC 2 cut(s) 300, 578
EcoT14I CCWWGG 1 cut(s) 728
EcoT22I ATGCAT 1 cut(s) 356
EcoT38I GRGCYC 2 cut(s) 65, 327
ErhI CCWWGG 1 cut(s) 728
FaqI GGGAC 4 cut(s) 194, 264, 579, 782
FauI CCCGC 3 cut(s) 42, 77, 287
FauNDI CATATG 1 cut(s) 931
Fnu4HI GCNGC 4 cut(s) 38, 245, 317, 924
FokI GGATG 3 cut(s) 501, 884, 989
FriOI GRGCYC 2 cut(s) 65, 327
Fsp4HI GCNGC 4 cut(s) 38, 245, 317, 924
GlaI GCGC 1 cut(s) 243
GluI GCNGC 4 cut(s) 38, 245, 317, 924
HaeIII GGCC 3 cut(s) 50, 273, 658
HapII CCGG 5 cut(s) 59, 248, 274, 389, 996
HhaI GCGC 1 cut(s) 244
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 2 cut(s) 494, 835
HindII GTYRAC 2 cut(s) 494, 835
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 3 cut(s) 395, 439, 503
HpaII CCGG 5 cut(s) 59, 248, 274, 389, 996
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 5 cut(s) 268, 494, 835, 917, 1081
Hpy188I TCNGA 2 cut(s) 46, 154
Hpy188III TCNNGA 5 cut(s) 389, 560, 581, 665, 910
Hpy8I GTNNAC 5 cut(s) 268, 494, 835, 917, 1081
HpyAV CCTTC 2 cut(s) 51, 274
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 2 cut(s) 264, 472
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 322, 547
HpyF3I CTNAG 3 cut(s) 45, 542, 1037
HpySE526I ACGT 2 cut(s) 264, 472
HspAI GCGC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 388
KpnI GGTACC 1 cut(s) 882
KroI GCCGGC 1 cut(s) 995
KroNI GCCGGC 1 cut(s) 997
Kzo9I GATC 4 cut(s) 30, 288, 946, 1045
LmnI GCTCC 2 cut(s) 68, 936
Lsp1109I GCAGC 2 cut(s) 24, 935
LweI GCATC 4 cut(s) 334, 345, 785, 967
MaeII ACGT 2 cut(s) 264, 472
MaeIII GTNAC 3 cut(s) 109, 500, 982
MalI GATC 4 cut(s) 32, 290, 948, 1047
MboI GATC 4 cut(s) 30, 288, 946, 1045
MboII GAAGA 5 cut(s) 289, 404, 428, 578, 581
MfeI CAATTG 2 cut(s) 16, 648
MflI RGATCY 2 cut(s) 30, 1045
MhlI GDGCHC 2 cut(s) 65, 327
MluCI AATT 6 cut(s) 16, 467, 648, 660, 687, 1052
MlyI GAGTC 2 cut(s) 389, 497
Mph1103I ATGCAT 1 cut(s) 356
MroI TCCGGA 1 cut(s) 388
MroNI GCCGGC 1 cut(s) 995
MseI TTAA 1 cut(s) 1055
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 5 cut(s) 59, 248, 274, 389, 996
MspR9I CCNGG 3 cut(s) 60, 249, 614
MunI CAATTG 2 cut(s) 16, 648
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 3 cut(s) 69, 322, 547
NaeI GCCGGC 1 cut(s) 997
NciI CCSGG 2 cut(s) 60, 249
NcoI CCATGG 1 cut(s) 728
NdeI CATATG 1 cut(s) 931
NdeII GATC 4 cut(s) 30, 288, 946, 1045
NgoMIV GCCGGC 1 cut(s) 995
NlaIV GGNNCC 5 cut(s) 64, 182, 712, 880, 1047
NmuCI GTSAC 1 cut(s) 500
NsiI ATGCAT 1 cut(s) 356
NspI RCATGY 3 cut(s) 358, 1003, 1065
NspV TTCGAA 2 cut(s) 606, 967
PaeI GCATGC 3 cut(s) 358, 1003, 1065
PagI TCATGA 2 cut(s) 559, 580
PasI CCCWGGG 1 cut(s) 613
PdiI GCCGGC 1 cut(s) 997
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 4 cut(s) 39, 246, 318, 925
PleI GAGTC 2 cut(s) 389, 497
PpsI GAGTC 2 cut(s) 389, 497
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 5 cut(s) 64, 182, 712, 880, 1047
PspPI GGNCC 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 828
PsuI RGATCY 2 cut(s) 30, 1045
RsaI GTAC 5 cut(s) 8, 269, 483, 880, 1082
RsaNI GTAC 5 cut(s) 7, 268, 482, 879, 1081
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 1055
SatI GCNGC 4 cut(s) 38, 245, 317, 924
Sau3AI GATC 4 cut(s) 30, 288, 946, 1045
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 2 cut(s) 389, 497
ScrFI CCNGG 3 cut(s) 60, 249, 614
SduI GDGCHC 2 cut(s) 65, 327
SfaNI GCATC 4 cut(s) 334, 345, 785, 967
SfcI CTRYAG 1 cut(s) 760
SfuI TTCGAA 2 cut(s) 606, 967
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 2 cut(s) 326, 599
SmoI CTYRAG 2 cut(s) 326, 599
SphI GCATGC 3 cut(s) 358, 1003, 1065
Sse9I AATT 6 cut(s) 16, 467, 648, 660, 687, 1052
SsiI CCGC 7 cut(s) 35, 70, 245, 257, 294, 317, 1014
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 3 cut(s) 58, 247, 612
StyI CCWWGG 1 cut(s) 728
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 2 cut(s) 267, 475
TaqI TCGA 5 cut(s) 381, 606, 909, 945, 967
TasI AATT 6 cut(s) 16, 467, 648, 660, 687, 1052
TauI GCSGC 2 cut(s) 247, 319
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 1 cut(s) 1055
Tru9I TTAA 1 cut(s) 1055
TscAI CASTG 2 cut(s) 418, 829
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 2 cut(s) 37, 923
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 4 cut(s) 579, 597, 671, 886
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 2 cut(s) 418, 829
VpaK11BI GGWCC 1 cut(s) 181
XceI RCATGY 3 cut(s) 358, 1003, 1065
Zsp2I ATGCAT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.