MD07G1094600.v1.1

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000588
Physical Location & Seq
Reverse (-)
10149775 .. 10153190
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1094600.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGTATAATATGCTGGTGAATTGCTGCCACTGCCGCACCCCTCTCCAGCTGCCCCCCGGTGCCGATTCCATCCGCTGCGCAATCTGCCAGGGTGTCACTATAATTGCGGACTCACGTGGCCTCCATCAGCCTCCAGCCTCCCACGCGCCTCCACCTCCCGCTCCTTACTCTCACGCCCCGTCACCTCCCTCCCCCTACTGTCACGCGCCTCCAGGCCCTCCCCCCAACACGCACGGGCGGAAGAAGGCTGTGATCTGCGGGATCTCGTATAAGTACTCGAGACATGAGCTCAAGGGTTGCATCAATGATGCCAAGTGCATGCGGTATCTCCTCATCAACAAGTTCCAGTTTCCAGACGATTGCATTGTCATGCTCACCGAAGAAGAAACTCACCCTTCCAAGATTCCGAACAGATACAACATTAGAATGGCATTACATTGGCTTGTACAAGGCTGTCAACCAGGAGACTCCCTCCTGTTTCATTACTCTGGTCATGGTTCACAGCAGAGGAATTATAATGGTGATGAAATTGATGGTTTTGATGAAACCCTTTGTCCCCTTGACTTCGAAACTCAGGGTATGATTGTTGATGATGAGATAAATGCAGCAATTGTAAGGCCCCTTCCACCCGGGGTTAAGCTTCATGCAATAATTGATGCTTGTCATAGTGGCACCGTGCTAGATTTGCCCTTCCTTTGCAGAATGGACAGGGGCGGACGATATGTATGGGAGGATCATCGCCCTCCATCAGGCATGTGGAAAGGATCAGGCGGTGGAGAAGTCATTTCCTTCAGTGGTTGTGATGATGATCAAACGTCTGCTGATACATCAGCTCTATCGAAGATCACATCAACAGGTGCTATGACCTTCTGCTTCATCCAAGCAATCGAGCGTGGACAAGCGAGTACCTATGGAAGCATACTCAATTCTATGCGCTCTACCATTCGAAGTACGGGTACGGGTGGTGGTGGTGGTGGTGGCGGCAGATTAACATCTCTCCTTGGTGGTGGTGGCGGCGGCGGCGGTGGTGCTGTGACATCCCTAGTCAGCATGCTTCTGACAGGAGGCAGTGCTACTGGCGGGCTAAAACAGGAACCGCAACTAACTGCCTGCGAGCCATTTGATGTGTATACTAAACCTTTCTCCCTATGA

Protein Analysis

384

Amino Acids

40.81

Weight (kDa)

6.38

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 8 - 32 2.6e-08 LSD1 zinc finger
Peptidase_C14 PF00656 80 - 361 3e-34 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
Acc16I TGCGCA 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 59, 671
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 1130
AccII CGCG 2 cut(s) 146, 206
AclWI GGATC 3 cut(s) 269, 741, 772
AcuI CTGAAG 1 cut(s) 775
AcvI CACGTG 1 cut(s) 116
AfaI GTAC 5 cut(s) 275, 447, 907, 952, 958
AfiI CCNNNNNNNGG 1 cut(s) 749
AjnI CCWGG 3 cut(s) 87, 211, 460
AluBI AGCT 4 cut(s) 49, 289, 640, 833
AluI AGCT 4 cut(s) 49, 289, 640, 833
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 274, 459
AlwI GGATC 3 cut(s) 269, 741, 772
Ama87I CYCGRG 2 cut(s) 277, 629
AoxI GGCC 3 cut(s) 118, 214, 617
ApeKI GCWGC 4 cut(s) 24, 49, 75, 605
AspLEI GCGC 4 cut(s) 80, 148, 208, 936
AspS9I GGNCC 2 cut(s) 215, 618
AsuC2I CCSGG 3 cut(s) 57, 630, 631
AsuHPI GGTGA 5 cut(s) 28, 174, 367, 383, 533
AsuII TTCGAA 2 cut(s) 567, 946
AvaI CYCGRG 2 cut(s) 277, 629
BanI GGYRCC 2 cut(s) 59, 671
BanII GRGCYC 1 cut(s) 291
BarI GAAGNNNNNNTAC 4 cut(s) 606, 638, 940, 972
BbrPI CACGTG 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 4 cut(s) 11, 36, 62, 617
BccI CCATC 4 cut(s) 77, 132, 527, 754
BciT130I CCWGG 3 cut(s) 89, 213, 462
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 3 cut(s) 57, 630, 631
BcoDI GTCTC 2 cut(s) 274, 459
BfaI CTAG 2 cut(s) 680, 1043
BisI GCNGC 9 cut(s) 25, 34, 50, 76, 606, 982, 1015, 1018, 1021
BlsI GCNGC 9 cut(s) 26, 35, 51, 77, 607, 983, 1016, 1019, 1022
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 6 cut(s) 57, 89, 213, 462, 630, 631
BmeT110I CYCGRG 2 cut(s) 277, 629
BmgT120I GGNCC 2 cut(s) 215, 618
BmiI GGNNCC 4 cut(s) 61, 620, 673, 1095
BmrFI CCNGG 6 cut(s) 57, 89, 213, 462, 630, 631
BmsI GCATC 3 cut(s) 298, 309, 646
BplI GAGNNNNNCTC 2 cut(s) 456, 488
BpmI CTGGAG 3 cut(s) 29, 117, 195
Bpu14I TTCGAA 2 cut(s) 567, 946
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 3 cut(s) 57, 630, 631
BsaAI YACGTR 1 cut(s) 116
BsaBI GATNNNNATC 2 cut(s) 807, 991
BsaJI CCNNGG 5 cut(s) 55, 88, 629, 630, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse1I ACTGG 2 cut(s) 346, 1081
Bse8I GATNNNNATC 2 cut(s) 807, 991
BseBI CCWGG 3 cut(s) 89, 213, 462
BseDI CCNNGG 5 cut(s) 55, 88, 629, 630, 1000
BseGI GGATG 3 cut(s) 69, 876, 1037
BseJI GATNNNNATC 2 cut(s) 807, 991
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 2 cut(s) 346, 1081
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 4 cut(s) 11, 36, 62, 617
Bsh1236I CGCG 2 cut(s) 146, 206
BshFI GGCC 3 cut(s) 120, 216, 619
BshNI GGYRCC 2 cut(s) 59, 671
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 2 cut(s) 277, 629
BsiSI CCGG 2 cut(s) 57, 630
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 2 cut(s) 274, 459
BsmFI GGGAC 1 cut(s) 540
BsnI GGCC 3 cut(s) 120, 216, 619
BsoBI CYCGRG 2 cut(s) 277, 629
Bsp119I TTCGAA 2 cut(s) 567, 946
Bsp1286I GDGCHC 1 cut(s) 291
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 6 cut(s) 252, 261, 733, 764, 808, 843
BspANI GGCC 3 cut(s) 120, 216, 619
BspCNI CTCAG 1 cut(s) 586
BspFNI CGCG 2 cut(s) 146, 206
BspLI GGNNCC 4 cut(s) 61, 620, 673, 1095
BspPI GGATC 3 cut(s) 269, 741, 772
BspT104I TTCGAA 2 cut(s) 567, 946
BspT107I GGYRCC 2 cut(s) 59, 671
BsrBI CCGCTC 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 2 cut(s) 346, 1081
BssECI CCNNGG 5 cut(s) 55, 88, 629, 630, 1000
BssMI GATC 6 cut(s) 252, 261, 733, 764, 808, 843
BssNAI GTATAC 1 cut(s) 1131
BssT1I CCWWGG 1 cut(s) 1000
Bst1107I GTATAC 1 cut(s) 1131
Bst2UI CCWGG 3 cut(s) 89, 213, 462
Bst4CI ACNGT 2 cut(s) 200, 676
BstAUI TGTACA 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 116
BstBI TTCGAA 2 cut(s) 567, 946
BstC8I GCNNGC 5 cut(s) 320, 1052, 1082, 1111, 1115
BstDEI CTNAG 1 cut(s) 573
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 69, 876, 1037
BstFNI CGCG 2 cut(s) 146, 206
BstHHI GCGC 4 cut(s) 80, 148, 208, 936
BstKTI GATC 6 cut(s) 255, 264, 736, 767, 811, 846
BstMAI GTCTC 2 cut(s) 274, 459
BstMBI GATC 6 cut(s) 252, 261, 733, 764, 808, 843
BstMWI GCNNNNNNNGC 6 cut(s) 30, 33, 84, 143, 685, 1020
BstNI CCWGG 3 cut(s) 89, 213, 462
BstNSI RCATGY 3 cut(s) 322, 757, 1054
BstSCI CCNGG 6 cut(s) 55, 87, 211, 460, 628, 629
BstUI CGCG 2 cut(s) 146, 206
BstV1I GCAGC 4 cut(s) 11, 36, 62, 617
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 1131
BsuRI GGCC 3 cut(s) 120, 216, 619
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 3 cut(s) 69, 876, 1037
BtsI GCAGTG 2 cut(s) 28, 1075
BtsIMutI CAGTG 3 cut(s) 28, 799, 1075
Cac8I GCNNGC 5 cut(s) 320, 1052, 1082, 1111, 1115
CfoI GCGC 4 cut(s) 80, 148, 208, 936
Cfr13I GGNCC 2 cut(s) 215, 618
Cfr9I CCCGGG 1 cut(s) 629
Csp6I GTAC 5 cut(s) 274, 446, 906, 951, 957
CviAII CATG 7 cut(s) 284, 319, 370, 494, 644, 754, 1051
CviQI GTAC 5 cut(s) 274, 446, 906, 951, 957
DdeI CTNAG 1 cut(s) 573
DpnI GATC 6 cut(s) 254, 263, 735, 766, 810, 845
DpnII GATC 6 cut(s) 252, 261, 733, 764, 808, 843
EciI GGCGGA 2 cut(s) 253, 729
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 1000
Eco24I GRGCYC 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 775
Eco72I CACGTG 1 cut(s) 116
Eco88I CYCGRG 2 cut(s) 277, 629
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoO109I RGGNCCY 2 cut(s) 215, 618
EcoRII CCWGG 3 cut(s) 87, 211, 460
EcoT14I CCWWGG 1 cut(s) 1000
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 1000
FaeI CATG 7 cut(s) 287, 322, 373, 497, 647, 757, 1054
FaqI GGGAC 1 cut(s) 540
FatI CATG 7 cut(s) 283, 318, 369, 493, 643, 753, 1050
FauI CCCGC 3 cut(s) 166, 251, 1073
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 1 cut(s) 1130
Fnu4HI GCNGC 9 cut(s) 25, 34, 50, 76, 606, 982, 1015, 1018, 1021
FokI GGATG 3 cut(s) 56, 863, 1024
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 9 cut(s) 25, 34, 50, 76, 606, 982, 1015, 1018, 1021
FspBI CTAG 2 cut(s) 680, 1043
FspI TGCGCA 1 cut(s) 79
GlaI GCGC 4 cut(s) 79, 147, 207, 935
GluI GCNGC 9 cut(s) 25, 34, 50, 76, 606, 982, 1015, 1018, 1021
GsuI CTGGAG 3 cut(s) 29, 117, 195
HaeIII GGCC 3 cut(s) 120, 216, 619
HapII CCGG 2 cut(s) 57, 630
HhaI GCGC 4 cut(s) 80, 148, 208, 936
Hin1II CATG 7 cut(s) 287, 322, 373, 497, 647, 757, 1054
Hin6I GCGC 4 cut(s) 78, 146, 206, 934
HinP1I GCGC 4 cut(s) 78, 146, 206, 934
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 4 cut(s) 65, 110, 403, 467
HpaII CCGG 2 cut(s) 57, 630
HphI GGTGA 5 cut(s) 28, 174, 367, 383, 533
Hpy166II GTNNAC 4 cut(s) 458, 500, 896, 1131
Hpy188I TCNGA 2 cut(s) 408, 1059
Hpy188III TCNNGA 2 cut(s) 279, 353
Hpy8I GTNNAC 4 cut(s) 458, 500, 896, 1131
HpyAV CCTTC 6 cut(s) 238, 405, 632, 700, 799, 877
HpyCH4III ACNGT 2 cut(s) 200, 676
HpyCH4IV ACGT 2 cut(s) 115, 815
HpyCH4V TGCA 6 cut(s) 300, 318, 363, 605, 647, 699
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 33, 84, 143, 685, 1020
HpyF3I CTNAG 1 cut(s) 573
HpySE526I ACGT 2 cut(s) 115, 815
Hsp92II CATG 7 cut(s) 287, 322, 373, 497, 647, 757, 1054
HspAI GCGC 4 cut(s) 78, 146, 206, 934
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 6 cut(s) 252, 261, 733, 764, 808, 843
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 4 cut(s) 11, 36, 62, 617
LweI GCATC 3 cut(s) 298, 309, 646
MaeI CTAG 2 cut(s) 680, 1043
MaeII ACGT 2 cut(s) 115, 815
MaeIII GTNAC 4 cut(s) 94, 180, 200, 1033
MalI GATC 6 cut(s) 254, 263, 735, 766, 810, 845
MbiI CCGCTC 1 cut(s) 161
MboI GATC 6 cut(s) 252, 261, 733, 764, 808, 843
MboII GAAGA 4 cut(s) 253, 392, 395, 853
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 7 cut(s) 19, 102, 511, 528, 609, 651, 925
MlyI GAGTC 2 cut(s) 104, 461
MseI TTAA 2 cut(s) 636, 989
MslI CAYNNNNRTG 2 cut(s) 368, 425
MspA1I CMGCKG 2 cut(s) 49, 75
MspI CCGG 2 cut(s) 57, 630
MspR9I CCNGG 6 cut(s) 57, 89, 213, 462, 630, 631
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 3 cut(s) 89, 213, 462
MvnI CGCG 2 cut(s) 146, 206
MwoI GCNNNNNNNGC 6 cut(s) 30, 33, 84, 143, 685, 1020
NciI CCSGG 3 cut(s) 57, 630, 631
NdeII GATC 6 cut(s) 252, 261, 733, 764, 808, 843
NlaIII CATG 7 cut(s) 287, 322, 373, 497, 647, 757, 1054
NlaIV GGNNCC 4 cut(s) 61, 620, 673, 1095
NmuCI GTSAC 4 cut(s) 94, 180, 200, 1033
NsbI TGCGCA 1 cut(s) 79
NspI RCATGY 3 cut(s) 322, 757, 1054
NspV TTCGAA 2 cut(s) 567, 946
PaeI GCATGC 2 cut(s) 322, 1054
PaeR7I CTCGAG 1 cut(s) 277
PfeI GAWTC 2 cut(s) 65, 403
PkrI GCNGC 9 cut(s) 26, 35, 51, 77, 607, 983, 1016, 1019, 1022
PleI GAGTC 2 cut(s) 104, 461
PmaCI CACGTG 1 cut(s) 116
PmlI CACGTG 1 cut(s) 116
PpsI GAGTC 2 cut(s) 104, 461
Ppu21I YACGTR 1 cut(s) 116
PsiI TTATAA 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 87, 211, 460
PspCI CACGTG 1 cut(s) 116
PspGI CCWGG 3 cut(s) 87, 211, 460
PspN4I GGNNCC 4 cut(s) 61, 620, 673, 1095
PspPI GGNCC 2 cut(s) 215, 618
PsuI RGATCY 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 5 cut(s) 275, 447, 907, 952, 958
RsaNI GTAC 5 cut(s) 274, 446, 906, 951, 957
RseI CAYNNNNRTG 2 cut(s) 368, 425
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 636, 989
SatI GCNGC 9 cut(s) 25, 34, 50, 76, 606, 982, 1015, 1018, 1021
Sau3AI GATC 6 cut(s) 252, 261, 733, 764, 808, 843
Sau96I GGNCC 2 cut(s) 215, 618
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 104, 461
ScrFI CCNGG 6 cut(s) 57, 89, 213, 462, 630, 631
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 3 cut(s) 298, 309, 646
Sfr274I CTCGAG 1 cut(s) 277
SfuI TTCGAA 2 cut(s) 567, 946
SlaI CTCGAG 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 368, 425
SmlI CTYRAG 2 cut(s) 277, 290
SmoI CTYRAG 2 cut(s) 277, 290
SphI GCATGC 2 cut(s) 322, 1054
Sse9I AATT 7 cut(s) 19, 102, 511, 528, 609, 651, 925
SspMI CTAG 2 cut(s) 680, 1043
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 6 cut(s) 55, 87, 211, 460, 628, 629
StyI CCWWGG 1 cut(s) 1000
TaaI ACNGT 2 cut(s) 200, 676
TaiI ACGT 2 cut(s) 118, 818
TaqI TCGA 5 cut(s) 278, 567, 839, 888, 946
TasI AATT 7 cut(s) 19, 102, 511, 528, 609, 651, 925
TatI WGTACW 2 cut(s) 273, 445
TauI GCSGC 5 cut(s) 36, 984, 1017, 1020, 1023
TfiI GAWTC 2 cut(s) 65, 403
Tru1I TTAA 2 cut(s) 636, 989
Tru9I TTAA 2 cut(s) 636, 989
TscAI CASTG 3 cut(s) 35, 799, 1075
TseFI GTSAC 4 cut(s) 94, 180, 200, 1033
TseI GCWGC 4 cut(s) 24, 49, 75, 605
Tsp45I GTSAC 4 cut(s) 94, 180, 200, 1033
TspDTI ATGAA 5 cut(s) 470, 540, 558, 632, 865
TspMI CCCGGG 1 cut(s) 629
TspRI CASTG 3 cut(s) 35, 799, 1075
XagI CCTNNNNNAGG 1 cut(s) 747
XceI RCATGY 3 cut(s) 322, 757, 1054
XcmI CCANNNNNNNNNTGG 1 cut(s) 753
XhoI CTCGAG 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 1130
XspI CTAG 2 cut(s) 680, 1043
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.