RLG00000028898

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000588
Physical Location & Seq
Forward (+)
29980231 .. 29983700
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028898

Sequence Viewer

Length: 1035 bp
ATGTACAGCAATATGCTGGTGAACTGCTCCAACTGCCACACCCCGCTCCAGCTTCCGACGGGCGCCCAGTCGATCCGCTGCGCCCTCTGCGACGCCGTCACCCTAATCGCGGACCCGCGCTCCCTGCACTCACTTCCGTCCTCCCACGCGCTCCCACCTCCCTCTCCTTACGGCCACGCGCCTCCCCCTCCTCCCTCGCCCTACAACCACGCGCCGCCGGGGCCTCCGCCGAGCGTGCACGGCCGGAAGAAGGCGGTGATCTGCGGGATATCGTATAAATACTCGATGCATGAGCTCAAGGGTTGCCTCAATGACGCCAGATGCATGCGGTATCTCCTCATCAACAAGTTCAAGTTTCCCGACGAATCCATTGTCATGCTCACTGAAGAAGAAACTCACCCAGCTAAGATTCCAAATAAATACAACATTAGAATGGCATTACATTGGCTTGTTCAAGGATGCCAACCAGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGACAGAGGGCTTATCATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTTGAAACCCAGGGTATGATTGTCGATGATGAGATAAATGCAACAATTGTTAGGCCCCTTCCACATGGGGTCAAGCTTCATGCAATTATAGACTCTTGTCATAGTGGCACTGTACTGGATTTGCCATTCCTTTGCAGAATGGACAGGGGTGGAAGATATGTATGGGAGGATCATCGCCCTCGATCAGGCTTATGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGATGATCAAACCTCTGCTGATACATCAGCTCTATCCAAGATCACATCAACTGGTGCCATGACTTTCTGCTTTATCCAAGCAATCGAGCGTGGACAGGCAGCCACATATGGGAGCATACTCAATTCTATGCGTAATACCATTCGAAGTACGGGTTCTGGTGGTGGTGGTGGTAGTGCTGTTACCTCTCTCCTTGGTGGTAGTGGAGGTGCTGTTACATCGCTTTAA

Protein Analysis

345

Amino Acids

37.01

Weight (kDa)

6.58

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 9 - 33 1.9e-10 LSD1 zinc finger
Peptidase_C14 PF00656 82 - 318 2.2e-33 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 863
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 5 cut(s) 110, 118, 149, 179, 212
AciI CCGC 9 cut(s) 44, 76, 110, 116, 215, 227, 254, 264, 328
AclWI GGATC 2 cut(s) 67, 747
AcoI YGGCCR 2 cut(s) 172, 241
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 3 cut(s) 63, 93, 315
AfaI GTAC 3 cut(s) 5, 684, 958
AfiI CCNNNNNNNGG 4 cut(s) 109, 250, 755, 918
AgsI TTSAA 3 cut(s) 352, 455, 575
AjnI CCWGG 2 cut(s) 466, 579
AluBI AGCT 5 cut(s) 52, 295, 404, 646, 839
AluI AGCT 5 cut(s) 52, 295, 404, 646, 839
Alw21I GWGCWC 2 cut(s) 240, 297
Alw44I GTGCAC 1 cut(s) 236
AlwI GGATC 2 cut(s) 67, 747
AoxI GGCC 4 cut(s) 172, 221, 241, 623
ApaLI GTGCAC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 78, 908
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AspLEI GCGC 6 cut(s) 65, 83, 120, 151, 181, 214
AspS9I GGNCC 3 cut(s) 112, 221, 624
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 6 cut(s) 31, 91, 268, 389, 482, 539
AsuII TTCGAA 1 cut(s) 952
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 2 cut(s) 62, 863
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 240, 297
BbvI GCAGC 2 cut(s) 65, 920
BccI CCATC 1 cut(s) 533
BceAI ACGGC 3 cut(s) 80, 187, 256
BciT130I CCWGG 2 cut(s) 468, 581
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 66
BglI GCCNNNNNGGC 1 cut(s) 220
BisI GCNGC 3 cut(s) 79, 215, 909
BlsI GCNGC 3 cut(s) 80, 216, 910
Bme1390I CCNGG 3 cut(s) 219, 468, 581
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 112, 221, 624
BmiI GGNNCC 5 cut(s) 64, 114, 222, 626, 865
BmrFI CCNGG 3 cut(s) 219, 468, 581
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 276, 311, 449
BmuI ACTGGG 1 cut(s) 61
BoxI GACNNNNGTC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 952
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 813
BsaHI GRCGYC 3 cut(s) 63, 93, 315
BsaJI CCNNGG 4 cut(s) 218, 579, 580, 1000
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 1005, 1035
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 250, 755, 918
Bse1I ACTGG 3 cut(s) 67, 690, 865
Bse8I GATNNNNATC 1 cut(s) 813
BseBI CCWGG 2 cut(s) 468, 581
BseDI CCNNGG 4 cut(s) 218, 579, 580, 1000
BseGI GGATG 1 cut(s) 464
BseJI GATNNNNATC 1 cut(s) 813
BseLI CCNNNNNNNGG 4 cut(s) 109, 250, 755, 918
BseNI ACTGG 3 cut(s) 67, 690, 865
BseRI GAGGAG 2 cut(s) 180, 326
BseSI GKGCMC 1 cut(s) 240
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 65, 920
BseYI CCCAGC 1 cut(s) 400
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 5 cut(s) 110, 118, 149, 179, 212
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 174, 223, 243, 625
BshNI GGYRCC 2 cut(s) 62, 863
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 240, 297
BsiSI CCGG 2 cut(s) 218, 244
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 4 cut(s) 109, 250, 755, 918
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 4 cut(s) 174, 223, 243, 625
Bsp119I TTCGAA 1 cut(s) 952
Bsp1286I GDGCHC 2 cut(s) 240, 297
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 72, 258, 739, 752, 814, 849
BspACI CCGC 9 cut(s) 44, 76, 110, 116, 215, 227, 254, 264, 328
BspANI GGCC 4 cut(s) 174, 223, 243, 625
BspFNI CGCG 5 cut(s) 110, 118, 149, 179, 212
BspLI GGNNCC 5 cut(s) 64, 114, 222, 626, 865
BspPI GGATC 2 cut(s) 67, 747
BspT104I TTCGAA 1 cut(s) 952
BspT107I GGYRCC 2 cut(s) 62, 863
BsrBI CCGCTC 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 67, 690, 865
BssECI CCNNGG 4 cut(s) 218, 579, 580, 1000
BssMI GATC 6 cut(s) 72, 258, 739, 752, 814, 849
BssNI GRCGYC 3 cut(s) 63, 93, 315
BssT1I CCWWGG 1 cut(s) 1000
Bst2UI CCWGG 2 cut(s) 468, 581
Bst4CI ACNGT 1 cut(s) 682
BstACI GRCGYC 3 cut(s) 63, 93, 315
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 952
BstC8I GCNNGC 2 cut(s) 236, 326
BstDEI CTNAG 1 cut(s) 405
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 1 cut(s) 464
BstFNI CGCG 5 cut(s) 110, 118, 149, 179, 212
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 6 cut(s) 65, 83, 120, 151, 181, 214
BstKTI GATC 6 cut(s) 75, 261, 742, 755, 817, 852
BstMBI GATC 6 cut(s) 72, 258, 739, 752, 814, 849
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 6 cut(s) 33, 87, 124, 220, 235, 240
BstNI CCWGG 2 cut(s) 468, 581
BstNSI RCATGY 1 cut(s) 328
BstPAI GACNNNNGTC 1 cut(s) 666
BstSCI CCNGG 3 cut(s) 217, 466, 579
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 5 cut(s) 110, 118, 149, 179, 212
BstV1I GCAGC 2 cut(s) 65, 920
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 4 cut(s) 174, 223, 243, 625
BtgZI GCGATG 2 cut(s) 728, 1011
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 3 cut(s) 381, 678, 805
Cac8I GCNNGC 2 cut(s) 236, 326
CfoI GCGC 6 cut(s) 65, 83, 120, 151, 181, 214
Cfr13I GGNCC 3 cut(s) 112, 221, 624
CseI GACGC 2 cut(s) 101, 323
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 3 cut(s) 4, 683, 957
CviAII CATG 8 cut(s) 290, 325, 376, 500, 524, 635, 650, 868
CviQI GTAC 3 cut(s) 4, 683, 957
DdeI CTNAG 1 cut(s) 405
DinI GGCGCC 1 cut(s) 64
DpnI GATC 6 cut(s) 74, 260, 741, 754, 816, 851
DpnII GATC 6 cut(s) 72, 258, 739, 752, 814, 849
EaeI YGGCCR 2 cut(s) 172, 241
EagI CGGCCG 1 cut(s) 241
EciI GGCGGA 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 295
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 1000
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoO109I RGGNCCY 2 cut(s) 221, 624
EcoRII CCWGG 2 cut(s) 466, 579
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 1000
EcoT22I ATGCAT 2 cut(s) 291, 326
EcoT38I GRGCYC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 1000
FaeI CATG 8 cut(s) 293, 328, 379, 503, 527, 638, 653, 871
FaqI GGGAC 1 cut(s) 546
FatI CATG 8 cut(s) 289, 324, 375, 499, 523, 634, 649, 867
FauI CCCGC 3 cut(s) 51, 123, 257
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 3 cut(s) 79, 215, 909
FokI GGATG 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 3 cut(s) 79, 215, 909
GlaI GCGC 6 cut(s) 64, 82, 119, 150, 180, 213
GluI GCNGC 3 cut(s) 79, 215, 909
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 32
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 174, 223, 243, 625
HapII CCGG 2 cut(s) 218, 244
HgaI GACGC 2 cut(s) 101, 323
HhaI GCGC 6 cut(s) 65, 83, 120, 151, 181, 214
Hin1I GRCGYC 3 cut(s) 63, 93, 315
Hin1II CATG 8 cut(s) 293, 328, 379, 503, 527, 638, 653, 871
Hin6I GCGC 6 cut(s) 63, 81, 118, 149, 179, 212
HinP1I GCGC 6 cut(s) 63, 81, 118, 149, 179, 212
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 4 cut(s) 365, 409, 473, 662
HpaII CCGG 2 cut(s) 218, 244
HphI GGTGA 6 cut(s) 31, 91, 268, 389, 482, 539
Hpy166II GTNNAC 4 cut(s) 22, 238, 506, 902
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 359, 507
Hpy8I GTNNAC 4 cut(s) 22, 238, 506, 902
Hpy99I CGWCG 3 cut(s) 61, 95, 365
HpyAV CCTTC 2 cut(s) 244, 638
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4V TGCA 7 cut(s) 127, 238, 289, 324, 611, 653, 705
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 87, 124, 220, 235, 240
HpyF3I CTNAG 1 cut(s) 405
Hsp92I GRCGYC 3 cut(s) 63, 93, 315
Hsp92II CATG 8 cut(s) 293, 328, 379, 503, 527, 638, 653, 871
HspAI GCGC 6 cut(s) 63, 81, 118, 149, 179, 212
KasI GGCGCC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 6 cut(s) 72, 258, 739, 752, 814, 849
LmnI GCTCC 5 cut(s) 32, 51, 125, 156, 921
Lsp1109I GCAGC 2 cut(s) 65, 920
LweI GCATC 3 cut(s) 276, 311, 449
MabI ACCWGGT 1 cut(s) 466
MaeIII GTNAC 4 cut(s) 97, 470, 988, 1021
MalI GATC 6 cut(s) 74, 260, 741, 754, 816, 851
MbiI CCGCTC 1 cut(s) 46
MboI GATC 6 cut(s) 72, 258, 739, 752, 814, 849
MboII GAAGA 4 cut(s) 259, 398, 401, 735
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 2 cut(s) 240, 297
MluCI AATT 3 cut(s) 615, 654, 931
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 2 cut(s) 467, 656
MmeI TCCRAC 2 cut(s) 54, 80
Mph1103I ATGCAT 2 cut(s) 291, 326
MseI TTAA 1 cut(s) 1033
MslI CAYNNNNRTG 3 cut(s) 374, 431, 528
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 2 cut(s) 218, 244
MspR9I CCNGG 3 cut(s) 219, 468, 581
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 2 cut(s) 468, 581
MvnI CGCG 5 cut(s) 110, 118, 149, 179, 212
MwoI GCNNNNNNNGC 6 cut(s) 33, 87, 124, 220, 235, 240
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 916
NdeII GATC 6 cut(s) 72, 258, 739, 752, 814, 849
NlaIII CATG 8 cut(s) 293, 328, 379, 503, 527, 638, 653, 871
NlaIV GGNNCC 5 cut(s) 64, 114, 222, 626, 865
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 97, 470
NsiI ATGCAT 2 cut(s) 291, 326
NspI RCATGY 1 cut(s) 328
NspV TTCGAA 1 cut(s) 952
PaeI GCATGC 1 cut(s) 328
PasI CCCWGGG 1 cut(s) 580
PfeI GAWTC 2 cut(s) 365, 409
PflFI GACNNNGTC 1 cut(s) 95
PkrI GCNGC 3 cut(s) 80, 216, 910
PleI GAGTC 2 cut(s) 467, 656
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 2 cut(s) 467, 656
PshAI GACNNNNGTC 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 466, 579
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 2 cut(s) 466, 579
PspN4I GGNNCC 5 cut(s) 64, 114, 222, 626, 865
PspPI GGNCC 3 cut(s) 112, 221, 624
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 3 cut(s) 5, 684, 958
RsaNI GTAC 3 cut(s) 4, 683, 957
RseI CAYNNNNRTG 3 cut(s) 374, 431, 528
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 1033
SatI GCNGC 3 cut(s) 79, 215, 909
Sau3AI GATC 6 cut(s) 72, 258, 739, 752, 814, 849
Sau96I GGNCC 3 cut(s) 112, 221, 624
SchI GAGTC 2 cut(s) 467, 656
ScrFI CCNGG 3 cut(s) 219, 468, 581
SduI GDGCHC 2 cut(s) 240, 297
SexAI ACCWGGT 1 cut(s) 466
SfaNI GCATC 3 cut(s) 276, 311, 449
SfoI GGCGCC 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 952
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 3 cut(s) 374, 431, 528
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 328
Sse9I AATT 3 cut(s) 615, 654, 931
SsiI CCGC 9 cut(s) 44, 76, 110, 116, 215, 227, 254, 264, 328
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 217, 466, 579
StyI CCWWGG 1 cut(s) 1000
TaaI ACNGT 1 cut(s) 682
TaqI TCGA 6 cut(s) 71, 284, 594, 751, 894, 952
TasI AATT 3 cut(s) 615, 654, 931
TatI WGTACW 2 cut(s) 3, 682
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 2 cut(s) 365, 409
Tru1I TTAA 1 cut(s) 1033
Tru9I TTAA 1 cut(s) 1033
TscAI CASTG 3 cut(s) 388, 685, 805
TseFI GTSAC 2 cut(s) 97, 470
TseI GCWGC 2 cut(s) 78, 908
Tsp45I GTSAC 2 cut(s) 97, 470
TspDTI ATGAA 3 cut(s) 546, 564, 638
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 3 cut(s) 388, 685, 805
Tth111I GACNNNGTC 1 cut(s) 95
VneI GTGCAC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 753
XceI RCATGY 1 cut(s) 328
Zsp2I ATGCAT 2 cut(s) 291, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.