RLG00000028885

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000588
Physical Location & Seq
Forward (+)
29776454 .. 29778993
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028885

Sequence Viewer

Length: 804 bp
ATGATGAGCAATAATGGTGGCCAACGACAACACAGTCGCAATTCCCAGACCCAGATCCCGCTACCTTCTGCGGTCAACAATGTCATTTTACTCTTTAATGTTTCTGGTAATCATGTTACTAATGTTTCTGATAATCAAGTTATTGGAAACACCGGGGACCATAATGGAAGTTTTTATGTTCGCCACACTCCCCCTCCCTTACCCTACAACCACGCGCCGCCGGGACCTCCGCCGAACGTGCACATGCCGAAGAAGGCTGTGATCTGCGCGATATCGTATAAAAAGTCGCCGCATGAGCTCAAGGGTCGCATCGATGACGCCAAACGCATGCGTAATCTCCTCATCAACAAGTTCGGGTTTCCAGAAGATTCCATTCTCATGCTCACTGAAGAAGAAACTGACCCACATAAGATTCCATATAAACGCAATATTGAAGAGGCATTACGTTGGCTTGTACAAGGATGTCAACCGGGTGACTCCCTTCTGTTTTACTTCTCTGGTCATGGTTCAAGGCTGGAGAAGCAGTATCCTGGTGATGAACTTGATGGATATGATGAAACCCTTTGGCCCCTTGACGTTAAAACCCAGGGTGTGATTCGTGATGGTTGGATAAATGCAACAATTGTTAGGCCAATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTGTTCTCATAGTGGCACCATACTGGATTTGCCATGGCTTTGCAGAATGGACGGGTACCATGACTTCCTGCTTCATCCAAGCAATCGAGAGTGGACATGCAGCCACATATGGGAGCTTACTCTATTTTATGCGTAA

Protein Analysis

268

Amino Acids

30.27

Weight (kDa)

6.56

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 84 - 234 1.5e-19 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 33
Acc65I GGTACC 1 cut(s) 723
AccB1I GGYRCC 2 cut(s) 683, 723
AccII CGCG 2 cut(s) 215, 269
AciI CCGC 5 cut(s) 59, 71, 218, 230, 290
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 2 cut(s) 456, 725
AfiI CCNNNNNNNGG 1 cut(s) 778
AgsI TTSAA 2 cut(s) 434, 510
AjnI CCWGG 2 cut(s) 529, 585
AluBI AGCT 3 cut(s) 298, 652, 784
AluI AGCT 3 cut(s) 298, 652, 784
Alw21I GWGCWC 2 cut(s) 243, 300
Alw44I GTGCAC 1 cut(s) 239
AlwI GGATC 1 cut(s) 49
AoxI GGCC 3 cut(s) 19, 566, 629
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 768
Asp718I GGTACC 1 cut(s) 723
AspLEI GCGC 2 cut(s) 217, 269
AspS9I GGNCC 3 cut(s) 157, 224, 567
AsuC2I CCSGG 3 cut(s) 154, 222, 471
AsuHPI GGTGA 2 cut(s) 485, 545
AvaII GGWCC 2 cut(s) 157, 224
BaeGI GKGCMC 1 cut(s) 243
BalI TGGCCA 1 cut(s) 21
BanI GGYRCC 2 cut(s) 683, 723
BanII GRGCYC 1 cut(s) 300
BarI GAAGNNNNNNTAC 4 cut(s) 426, 458, 716, 748
Bbv12I GWGCWC 2 cut(s) 243, 300
BbvI GCAGC 1 cut(s) 780
BccI CCATC 2 cut(s) 539, 596
BciT130I CCWGG 2 cut(s) 531, 587
BciVI GTATCC 1 cut(s) 537
BcnI CCSGG 3 cut(s) 154, 222, 471
BfuI GTATCC 1 cut(s) 537
BisI GCNGC 3 cut(s) 218, 290, 769
BlsI GCNGC 3 cut(s) 219, 291, 770
Bme1390I CCNGG 5 cut(s) 154, 222, 471, 531, 587
Bme18I GGWCC 2 cut(s) 157, 224
BmgT120I GGNCC 3 cut(s) 157, 224, 567
BmiI GGNNCC 5 cut(s) 158, 225, 569, 685, 725
BmrFI CCNGG 5 cut(s) 154, 222, 471, 531, 587
BmsI GCATC 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 3 cut(s) 154, 222, 471
Bsa29I ATCGAT 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 153, 585, 586, 701
BsaXI ACNNNNNCTCC 4 cut(s) 178, 208, 509, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 778
Bse1I ACTGG 1 cut(s) 696
BseBI CCWGG 2 cut(s) 531, 587
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 4 cut(s) 153, 585, 586, 701
BseGI GGATG 2 cut(s) 467, 742
BseLI CCNNNNNNNGG 1 cut(s) 778
BseNI ACTGG 1 cut(s) 696
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 1 cut(s) 780
Bsh1236I CGCG 2 cut(s) 215, 269
BshFI GGCC 3 cut(s) 21, 568, 631
BshNI GGYRCC 2 cut(s) 683, 723
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 2 cut(s) 243, 300
BsiSI CCGG 3 cut(s) 153, 221, 470
BslFI GGGAC 2 cut(s) 170, 237
BslI CCNNNNNNNGG 1 cut(s) 778
BsmFI GGGAC 2 cut(s) 170, 237
BsnI GGCC 3 cut(s) 21, 568, 631
Bsp1286I GDGCHC 2 cut(s) 243, 300
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 54, 261
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 5 cut(s) 59, 71, 218, 230, 290
BspANI GGCC 3 cut(s) 21, 568, 631
BspDI ATCGAT 1 cut(s) 312
BspFNI CGCG 2 cut(s) 215, 269
BspLI GGNNCC 5 cut(s) 158, 225, 569, 685, 725
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 2 cut(s) 683, 723
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 696
BssECI CCNNGG 4 cut(s) 153, 585, 586, 701
BssMI GATC 2 cut(s) 54, 261
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 701
Bst2UI CCWGG 2 cut(s) 531, 587
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 329
BstDSI CCRYGG 1 cut(s) 701
BstF5I GGATG 2 cut(s) 467, 742
BstFNI CGCG 2 cut(s) 215, 269
BstHHI GCGC 2 cut(s) 217, 269
BstKTI GATC 2 cut(s) 57, 264
BstMBI GATC 2 cut(s) 54, 261
BstMWI GCNNNNNNNGC 3 cut(s) 238, 295, 520
BstNI CCWGG 2 cut(s) 531, 587
BstNSI RCATGY 3 cut(s) 247, 331, 768
BstSCI CCNGG 5 cut(s) 152, 220, 469, 529, 585
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 2 cut(s) 215, 269
BstV1I GCAGC 1 cut(s) 780
BstX2I RGATCY 1 cut(s) 54
BstYI RGATCY 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 312
BsuI GTATCC 1 cut(s) 537
BsuRI GGCC 3 cut(s) 21, 568, 631
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 2 cut(s) 467, 742
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 1 cut(s) 329
CfoI GCGC 2 cut(s) 217, 269
Cfr13I GGNCC 3 cut(s) 157, 224, 567
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 455, 724
CspCI CAANNNNNGTGG 1 cut(s) 33
CviQI GTAC 2 cut(s) 455, 724
DpnI GATC 2 cut(s) 56, 263
DpnII GATC 2 cut(s) 54, 261
DrdI GACNNNNNNGTC 1 cut(s) 33
DseDI GACNNNNNNGTC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 273
Eco47I GGWCC 2 cut(s) 157, 224
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 529, 585
EcoRV GATATC 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 701
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 701
FaqI GGGAC 2 cut(s) 170, 237
FauI CCCGC 1 cut(s) 66
FauNDI CATATG 1 cut(s) 776
Fnu4HI GCNGC 3 cut(s) 218, 290, 769
FokI GGATG 2 cut(s) 474, 729
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 218, 290, 769
GlaI GCGC 2 cut(s) 216, 268
GluI GCNGC 3 cut(s) 218, 290, 769
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 3 cut(s) 21, 568, 631
HapII CCGG 3 cut(s) 153, 221, 470
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 217, 269
Hin1I GRCGYC 1 cut(s) 318
Hin6I GCGC 2 cut(s) 215, 267
HinP1I GCGC 2 cut(s) 215, 267
HincII GTYRAC 2 cut(s) 76, 467
HindII GTYRAC 2 cut(s) 76, 467
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 4 cut(s) 368, 412, 476, 595
HpaII CCGG 3 cut(s) 153, 221, 470
HphI GGTGA 2 cut(s) 485, 545
Hpy166II GTNNAC 4 cut(s) 76, 241, 467, 762
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 362, 599, 755
Hpy8I GTNNAC 4 cut(s) 76, 241, 467, 762
HpyAV CCTTC 3 cut(s) 75, 247, 491
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 3 cut(s) 237, 445, 576
HpyCH4V TGCA 5 cut(s) 241, 617, 659, 711, 768
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 295, 520
HpySE526I ACGT 3 cut(s) 237, 445, 576
Hsp92I GRCGYC 1 cut(s) 318
HspAI GCGC 2 cut(s) 215, 267
KpnI GGTACC 1 cut(s) 727
Kzo9I GATC 2 cut(s) 54, 261
LmnI GCTCC 1 cut(s) 781
Lsp1109I GCAGC 1 cut(s) 780
LweI GCATC 1 cut(s) 318
MaeII ACGT 3 cut(s) 237, 445, 576
MaeIII GTNAC 2 cut(s) 115, 473
MalI GATC 2 cut(s) 56, 263
MboI GATC 2 cut(s) 54, 261
MboII GAAGA 5 cut(s) 262, 377, 401, 404, 446
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 2 cut(s) 243, 300
MlsI TGGCCA 1 cut(s) 21
MluCI AATT 4 cut(s) 40, 621, 633, 660
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 4 cut(s) 204, 237, 350, 430
Mox20I TGGCCA 1 cut(s) 21
MscI TGGCCA 1 cut(s) 21
MseI TTAA 2 cut(s) 96, 579
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 21
MspI CCGG 3 cut(s) 153, 221, 470
MspR9I CCNGG 5 cut(s) 154, 222, 471, 531, 587
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 2 cut(s) 531, 587
MvnI CGCG 2 cut(s) 215, 269
MwoI GCNNNNNNNGC 3 cut(s) 238, 295, 520
NciI CCSGG 3 cut(s) 154, 222, 471
NcoI CCATGG 1 cut(s) 701
NdeI CATATG 1 cut(s) 776
NdeII GATC 2 cut(s) 54, 261
NlaIV GGNNCC 5 cut(s) 158, 225, 569, 685, 725
NmuCI GTSAC 1 cut(s) 473
NspI RCATGY 3 cut(s) 247, 331, 768
PaeI GCATGC 1 cut(s) 331
PasI CCCWGGG 1 cut(s) 586
PfeI GAWTC 3 cut(s) 368, 412, 595
PkrI GCNGC 3 cut(s) 219, 291, 770
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PpuMI RGGWCCY 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 529, 585
PspGI CCWGG 2 cut(s) 529, 585
PspN4I GGNNCC 5 cut(s) 158, 225, 569, 685, 725
PspPI GGNCC 3 cut(s) 157, 224, 567
PspPPI RGGWCCY 1 cut(s) 224
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 2 cut(s) 456, 725
RsaNI GTAC 2 cut(s) 455, 724
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 96, 579
SatI GCNGC 3 cut(s) 218, 290, 769
Sau3AI GATC 2 cut(s) 54, 261
Sau96I GGNCC 3 cut(s) 157, 224, 567
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 5 cut(s) 154, 222, 471, 531, 587
SduI GDGCHC 2 cut(s) 243, 300
SetI ASST 8 cut(s) 67, 229, 240, 300, 448, 579, 654, 786
SfaNI GCATC 1 cut(s) 318
SinI GGWCC 2 cut(s) 157, 224
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SphI GCATGC 1 cut(s) 331
Sse9I AATT 4 cut(s) 40, 621, 633, 660
SsiI CCGC 5 cut(s) 59, 71, 218, 230, 290
SspI AATATT 1 cut(s) 430
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 5 cut(s) 152, 220, 469, 529, 585
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 3 cut(s) 240, 448, 579
TaqI TCGA 2 cut(s) 312, 754
TasI AATT 4 cut(s) 40, 621, 633, 660
TatI WGTACW 1 cut(s) 454
TauI GCSGC 2 cut(s) 220, 292
TfiI GAWTC 3 cut(s) 368, 412, 595
Tru1I TTAA 2 cut(s) 96, 579
Tru9I TTAA 2 cut(s) 96, 579
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 1 cut(s) 768
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 552, 570, 644, 731
TspRI CASTG 1 cut(s) 391
VneI GTGCAC 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 157, 224
XceI RCATGY 3 cut(s) 247, 331, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.