RchiOBHm_Chr1g0344901

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000588
Physical Location & Seq
Reverse (-)
37250831 .. 37254092
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57130

Sequence Viewer

Length: 1104 bp
ATGATGATGAGCAATTATGGACGCCAACGACAACTAGTGGTCGACTGTTGGTATTGCCAGACCCAAATCCCGCTGCCTTCTGTGAGTCCGATATCCGTCCGCTGCCGCCGCTGCTATGCCAGTACCGGCGTCGCCTCTCCGGGCTTCCCCCGTTCCCCCTATAGTGCTGTACCCTACTATGTCCGTATAAACCCACCACCACACGCCGGCTTCCCCCAAAGATCACCAGGACCTCCGCAGAACGTGCACAGGCGGAAGAAGGCGGTGATCTGCGGGATATCGTATCTCTACACGAGGACTGAGCTCAACGGTTGCATCAATGACGCCAAACGCATGCGGACTCTCCTCATCAGCAAGTTCAATTTTCCAGAAGATTCCATTGTCATGCTCACTGAAAAAGAAACTGATCCATATAACAAACCATATAAAAACAACATTAAAAGGGAATTACGTTGGCTTGTGCAAGGATGTGAACCCGGTGACTCCCTTCTGTTTTACTACTCTGGTCATGGTTCACAGCTGCCGGAGCGGCATCTAGGTGATGAAGATGATGAAATGGATGAAACCCTTTGTCCCCTTGACGTCGAAACCCAGGGTATGATTCGTGATGATTGGATAAATGCAACAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTCTTGTCATAGTGGCACCATACTGGATTTGCCATGGCTTTGCAATATGGACTGGGATGGAAACTATGAATGGGACGATCATAGGCCTAAATCAGGCGTATACAAAGGAGCAAGTGGTGGAGAAGTTATATGTATCAGTGGTTGTGATGACCATCAAACCTCTGAGGAAACACCATCAATCCCAACAACAGGTGTCATGACTTCCTGCTTCATCCAAGCAATCAAGAGTGGACAGGCAGCCACATATGGGAGCTTACTCTATTATATGCGTAATAACATTCGTAAGGTTTCTGGTGGTGGTGGTGCTGTTACATCTCTTGACAGCATGCTTCCAACAGGCGGAAGTGTTGGTGGTGGTAGGCTAAGACAGGAGCCTCAATTAACTGCCGGCAAGCGGTTCGATGTGTACACGAAACCTTTTACCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

367

Amino Acids

40.94

Weight (kDa)

7.51

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 85 - 354 1.7e-33 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 585
AccB1I GGYRCC 1 cut(s) 689
AccBSI CCGCTC 1 cut(s) 529
AccI GTMKAC 2 cut(s) 42, 774
AclWI GGATC 1 cut(s) 401
AcyI GRCGYC 4 cut(s) 22, 129, 324, 582
AfaI GTAC 3 cut(s) 124, 171, 1082
AfiI CCNNNNNNNGG 6 cut(s) 206, 767, 863, 921, 1013, 1068
AgsI TTSAA 1 cut(s) 361
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 2 cut(s) 226, 591
AluBI AGCT 4 cut(s) 304, 520, 658, 927
AluI AGCT 4 cut(s) 304, 520, 658, 927
Alw21I GWGCWC 2 cut(s) 249, 306
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 1 cut(s) 401
AoxI GGCC 2 cut(s) 635, 758
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 5 cut(s) 73, 102, 111, 520, 911
AspS9I GGNCC 2 cut(s) 230, 636
AsuC2I CCSGG 2 cut(s) 141, 477
AsuHPI GGTGA 4 cut(s) 216, 277, 491, 551
AvaII GGWCC 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 689
BanII GRGCYC 1 cut(s) 306
BauI CACGAG 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 249, 306
BbvI GCAGC 5 cut(s) 60, 89, 98, 507, 923
BccI CCATC 3 cut(s) 725, 834, 856
BcgI CGANNNNNNTGC 2 cut(s) 1054, 1088
BciT130I CCWGG 2 cut(s) 228, 593
BcnI CCSGG 2 cut(s) 141, 477
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 536
BfmI CTRYAG 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 529
BisI GCNGC 8 cut(s) 74, 103, 106, 109, 112, 521, 530, 912
BlsI GCNGC 8 cut(s) 75, 104, 107, 110, 113, 522, 531, 913
Bme1390I CCNGG 4 cut(s) 141, 228, 477, 593
Bme18I GGWCC 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 230, 636
BmiI GGNNCC 2 cut(s) 691, 1047
BmrFI CCNGG 4 cut(s) 141, 228, 477, 593
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 324, 541
BmuI ACTGGG 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 141, 477
BsaBI GATNNNNATC 1 cut(s) 825
BsaHI GRCGYC 4 cut(s) 22, 129, 324, 582
BsaJI CCNNGG 3 cut(s) 591, 592, 707
Bsc4I CCNNNNNNNGG 6 cut(s) 206, 767, 863, 921, 1013, 1068
Bse118I RCCGGY 3 cut(s) 125, 206, 1061
Bse1I ACTGG 3 cut(s) 120, 702, 731
Bse8I GATNNNNATC 1 cut(s) 825
BseBI CCWGG 2 cut(s) 228, 593
BseDI CCNNGG 3 cut(s) 591, 592, 707
BseGI GGATG 4 cut(s) 473, 565, 736, 885
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 6 cut(s) 206, 767, 863, 921, 1013, 1068
BseMII CTCAG 2 cut(s) 291, 828
BseNI ACTGG 3 cut(s) 120, 702, 731
BseRI GAGGAG 1 cut(s) 335
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 5 cut(s) 60, 89, 98, 507, 923
BshFI GGCC 2 cut(s) 637, 760
BshNI GGYRCC 1 cut(s) 689
BsiHKAI GWGCWC 2 cut(s) 249, 306
BsiSI CCGG 6 cut(s) 126, 140, 207, 477, 524, 1062
BslFI GGGAC 2 cut(s) 558, 761
BslI CCNNNNNNNGG 6 cut(s) 206, 767, 863, 921, 1013, 1068
BsmFI GGGAC 2 cut(s) 558, 761
BsnI GGCC 2 cut(s) 637, 760
Bsp1286I GDGCHC 2 cut(s) 249, 306
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 4 cut(s) 221, 267, 406, 751
Bsp19I CCATGG 1 cut(s) 707
BspANI GGCC 2 cut(s) 637, 760
BspCNI CTCAG 2 cut(s) 292, 829
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 2 cut(s) 691, 1047
BspPI GGATC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 689
BsrBI CCGCTC 1 cut(s) 529
BsrFI RCCGGY 3 cut(s) 125, 206, 1061
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 3 cut(s) 120, 702, 731
BssAI RCCGGY 3 cut(s) 125, 206, 1061
BssECI CCNNGG 3 cut(s) 591, 592, 707
BssMI GATC 4 cut(s) 221, 267, 406, 751
BssNAI GTATAC 1 cut(s) 775
BssNI GRCGYC 4 cut(s) 22, 129, 324, 582
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 707
Bst1107I GTATAC 1 cut(s) 775
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 228, 593
Bst4CI ACNGT 2 cut(s) 47, 311
BstACI GRCGYC 4 cut(s) 22, 129, 324, 582
BstAPI GCANNNNNTGC 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 1080
BstC8I GCNNGC 5 cut(s) 208, 335, 1001, 1063, 1067
BstDEI CTNAG 3 cut(s) 300, 837, 1037
BstDSI CCRYGG 1 cut(s) 707
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 4 cut(s) 473, 565, 736, 885
BstKTI GATC 4 cut(s) 224, 270, 409, 754
BstMBI GATC 4 cut(s) 221, 267, 406, 751
BstMWI GCNNNNNNNGC 5 cut(s) 108, 111, 244, 526, 529
BstNI CCWGG 2 cut(s) 228, 593
BstNSI RCATGY 2 cut(s) 337, 1003
BstSCI CCNGG 4 cut(s) 139, 226, 475, 591
BstSFI CTRYAG 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 5 cut(s) 60, 89, 98, 507, 923
BstZ17I GTATAC 1 cut(s) 775
BsuRI GGCC 2 cut(s) 637, 760
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 4 cut(s) 473, 565, 736, 885
BtsIMutI CAGTG 2 cut(s) 390, 817
Cac8I GCNNGC 5 cut(s) 208, 335, 1001, 1063, 1067
CciI TCATGA 1 cut(s) 870
Cfr10I RCCGGY 3 cut(s) 125, 206, 1061
Cfr13I GGNCC 2 cut(s) 230, 636
CseI GACGC 3 cut(s) 30, 118, 332
Csp6I GTAC 3 cut(s) 123, 170, 1081
CviAII CATG 8 cut(s) 334, 385, 509, 647, 662, 708, 871, 1000
CviQI GTAC 3 cut(s) 123, 170, 1081
DdeI CTNAG 3 cut(s) 300, 837, 1037
DpnI GATC 4 cut(s) 223, 269, 408, 753
DpnII GATC 4 cut(s) 221, 267, 406, 751
EciI GGCGGA 2 cut(s) 268, 1029
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 707
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 306
Eco32I GATATC 2 cut(s) 93, 279
Eco47I GGWCC 1 cut(s) 230
Eco53kI GAGCTC 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 226, 591
EcoRV GATATC 2 cut(s) 93, 279
EcoT14I CCWWGG 1 cut(s) 707
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 8 cut(s) 337, 388, 512, 650, 665, 711, 874, 1003
FaqI GGGAC 2 cut(s) 558, 761
FatI CATG 8 cut(s) 333, 384, 508, 646, 661, 707, 870, 999
FauI CCCGC 2 cut(s) 78, 266
FauNDI CATATG 1 cut(s) 919
FblI GTMKAC 2 cut(s) 42, 774
Fnu4HI GCNGC 8 cut(s) 74, 103, 106, 109, 112, 521, 530, 912
FokI GGATG 4 cut(s) 480, 572, 743, 872
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 8 cut(s) 74, 103, 106, 109, 112, 521, 530, 912
FspBI CTAG 2 cut(s) 35, 536
GluI GCNGC 8 cut(s) 74, 103, 106, 109, 112, 521, 530, 912
HaeIII GGCC 2 cut(s) 637, 760
HapII CCGG 6 cut(s) 126, 140, 207, 477, 524, 1062
HgaI GACGC 3 cut(s) 30, 118, 332
Hin1I GRCGYC 4 cut(s) 22, 129, 324, 582
Hin1II CATG 8 cut(s) 337, 388, 512, 650, 665, 711, 874, 1003
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 6 cut(s) 85, 340, 374, 482, 601, 674
HpaII CCGG 6 cut(s) 126, 140, 207, 477, 524, 1062
HphI GGTGA 4 cut(s) 216, 277, 491, 551
Hpy166II GTNNAC 8 cut(s) 43, 247, 473, 515, 775, 905, 1081, 1083
Hpy188I TCNGA 2 cut(s) 90, 838
Hpy188III TCNNGA 5 cut(s) 368, 605, 871, 898, 992
Hpy8I GTNNAC 8 cut(s) 43, 247, 473, 515, 775, 905, 1081, 1083
Hpy99I CGWCG 2 cut(s) 134, 587
HpyAV CCTTC 3 cut(s) 87, 253, 497
HpyCH4III ACNGT 2 cut(s) 47, 311
HpyCH4IV ACGT 3 cut(s) 243, 451, 582
HpyCH4V TGCA 6 cut(s) 247, 315, 463, 623, 665, 717
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 111, 244, 526, 529
HpyF3I CTNAG 3 cut(s) 300, 837, 1037
HpySE526I ACGT 3 cut(s) 243, 451, 582
Hsp92I GRCGYC 4 cut(s) 22, 129, 324, 582
Hsp92II CATG 8 cut(s) 337, 388, 512, 650, 665, 711, 874, 1003
KroI GCCGGC 2 cut(s) 206, 1061
KroNI GCCGGC 2 cut(s) 208, 1063
Kzo9I GATC 4 cut(s) 221, 267, 406, 751
LmnI GCTCC 4 cut(s) 526, 782, 924, 1045
Lsp1109I GCAGC 5 cut(s) 60, 89, 98, 507, 923
LweI GCATC 2 cut(s) 324, 541
MaeI CTAG 2 cut(s) 35, 536
MaeII ACGT 3 cut(s) 243, 451, 582
MaeIII GTNAC 2 cut(s) 479, 982
MalI GATC 4 cut(s) 223, 269, 408, 753
MbiI CCGCTC 1 cut(s) 529
MboI GATC 4 cut(s) 221, 267, 406, 751
MboII GAAGA 3 cut(s) 268, 383, 557
MfeI CAATTG 1 cut(s) 627
MhlI GDGCHC 2 cut(s) 249, 306
MluCI AATT 6 cut(s) 13, 361, 446, 627, 666, 1052
MlyI GAGTC 3 cut(s) 94, 334, 476
MmeI TCCRAC 1 cut(s) 1031
MnlI CCTC 7 cut(s) 145, 243, 288, 356, 832, 844, 1059
MroNI GCCGGC 2 cut(s) 206, 1061
MseI TTAA 2 cut(s) 438, 1055
MslI CAYNNNNRTG 2 cut(s) 383, 537
MspA1I CMGCKG 4 cut(s) 73, 102, 111, 520
MspI CCGG 6 cut(s) 126, 140, 207, 477, 524, 1062
MspR9I CCNGG 4 cut(s) 141, 228, 477, 593
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 2 cut(s) 228, 593
MwoI GCNNNNNNNGC 5 cut(s) 108, 111, 244, 526, 529
NaeI GCCGGC 2 cut(s) 208, 1063
NciI CCSGG 2 cut(s) 141, 477
NcoI CCATGG 1 cut(s) 707
NdeI CATATG 1 cut(s) 919
NdeII GATC 4 cut(s) 221, 267, 406, 751
NgoMIV GCCGGC 2 cut(s) 206, 1061
NlaIII CATG 8 cut(s) 337, 388, 512, 650, 665, 711, 874, 1003
NlaIV GGNNCC 2 cut(s) 691, 1047
NmuCI GTSAC 1 cut(s) 479
NspI RCATGY 2 cut(s) 337, 1003
PaeI GCATGC 2 cut(s) 337, 1003
PagI TCATGA 1 cut(s) 870
PasI CCCWGGG 1 cut(s) 592
PceI AGGCCT 1 cut(s) 760
PdiI GCCGGC 2 cut(s) 208, 1063
PfeI GAWTC 3 cut(s) 374, 601, 674
PkrI GCNGC 8 cut(s) 75, 104, 107, 110, 113, 522, 531, 913
PleI GAGTC 3 cut(s) 93, 334, 476
PpsI GAGTC 3 cut(s) 93, 334, 476
PpuMI RGGWCCY 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 226, 591
PspGI CCWGG 2 cut(s) 226, 591
PspN4I GGNNCC 2 cut(s) 691, 1047
PspPI GGNCC 2 cut(s) 230, 636
PspPPI RGGWCCY 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 3 cut(s) 124, 171, 1082
RsaNI GTAC 3 cut(s) 123, 170, 1081
RseI CAYNNNNRTG 2 cut(s) 383, 537
SacI GAGCTC 1 cut(s) 306
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 438, 1055
SatI GCNGC 8 cut(s) 74, 103, 106, 109, 112, 521, 530, 912
Sau3AI GATC 4 cut(s) 221, 267, 406, 751
Sau96I GGNCC 2 cut(s) 230, 636
SchI GAGTC 3 cut(s) 94, 334, 476
ScrFI CCNGG 4 cut(s) 141, 228, 477, 593
SduI GDGCHC 2 cut(s) 249, 306
SfaNI GCATC 2 cut(s) 324, 541
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 383, 537
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 2 cut(s) 337, 1003
Sse9I AATT 6 cut(s) 13, 361, 446, 627, 666, 1052
SseBI AGGCCT 1 cut(s) 760
SspMI CTAG 2 cut(s) 35, 536
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 4 cut(s) 139, 226, 475, 591
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 2 cut(s) 47, 311
TaiI ACGT 3 cut(s) 246, 454, 585
TaqI TCGA 3 cut(s) 42, 585, 1074
TasI AATT 6 cut(s) 13, 361, 446, 627, 666, 1052
TatI WGTACW 1 cut(s) 1080
TauI GCSGC 3 cut(s) 108, 111, 532
TfiI GAWTC 3 cut(s) 374, 601, 674
Tru1I TTAA 2 cut(s) 438, 1055
Tru9I TTAA 2 cut(s) 438, 1055
TscAI CASTG 2 cut(s) 397, 817
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 5 cut(s) 73, 102, 111, 520, 911
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 6 cut(s) 558, 567, 576, 650, 756, 874
TspGWI ACGGA 2 cut(s) 85, 173
TspRI CASTG 2 cut(s) 397, 817
VneI GTGCAC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 230
XagI CCTNNNNNAGG 1 cut(s) 765
XceI RCATGY 2 cut(s) 337, 1003
XmiI GTMKAC 2 cut(s) 42, 774
XspI CTAG 2 cut(s) 35, 536
ZraI GACGTC 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.