Rh1DG189400

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000588
Physical Location & Seq
Reverse (-)
37823258 .. 37917430
94173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG189400.1

Sequence Viewer

Length: 1230 bp
ATGCACCAGTTCATGTCCCCAGTATCTATCTCTCTTTGTTCGTCTCAAAAATCGTTACATTCAAACATTCACTCCCATTCAACTCCTGCATGCACTTCTCCTCCAGATTCCATGATGATGAGCAATTATGGAGGCCAACGACTACTAGTGGTCGACTGTTGCTATTGCCAGACCCAAATCCAGCTGCCTTCTGTGAGTCCTATATACATCGTCGGCACATCCGTCCGCTGCGGCCGCTGCTATGCCAGTACCCGCGTCGCCTCTCCGGGCTTCCCCCGCTCCCCTTATAGTGCTGTACCCTACTATGTCCCTATAAACCCACCACCACACGCCGGCTTCCCCCACAGATCACCAGGACCTCCGCCGAACGTGCACAGGCAGAAGAAGGCGGTGATCTGCGGGATATCGTATAGGTACTCGAGGCATGAGCTCAAAGGTAGCATCAATGACGCCAAAAACATGCGGTATCTCCTCATGAGCAAGTTCAAGTTTCCAGAAGATTCCATTTGCATGCTCACTGAAGAAGAAACTGACCCATGTAAGATTCCCTATAAAAACAACATTGAAAGGGAATTACGTTGGCTTGTACGAGGATGTCAACATGGTGACTCCCTTCTGTTTTACTTCTCTGGTCATGTTTCAAGGCTGAGGAAGCCGTATCCTCGTGATGAAGAAGATAGATATCATGAAACCCTCTGTCCCCTTGAGTTCGAAACCCAGGGTATGATTGTTGATGATTGGATAAATGCAACAATTGTTAAGCCAATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTGTTCTCATAGTGGCACCATACTGGATTTGCCATGGCTTTTCAGAATGGACGGGGGTGGAAACTATACATGGGACAAGGCATCAAGCGTATGGAAAGGAACAAGTGGTGGAGAAGTTATATGTATCAGTGGCTGTGTTGACCATCAAACCTCTGCGGAAACACAATCAATCCCAGCAACAGGTGTCATGACTTCCTGCTTCATCCAAGCAATCAAGAGTGGACAGGCAGCCACATATGGGAGCTTACTCTATTTTATGCGTAATACCATTCGAAGTGGTGGTGGTGGTGGTGGTACTGGTGGTGCTGTTACATCTCTTGCCGGCATGCTTCTAGCAGGCGGAAGTGTTGGTGGTGGTGGACGGAGACAGGAGCCTCAATTAACTGCCTGCGACCCGTTGGATGTGTACACGAAACCTTTTGCCCTATGA

Protein Analysis

409

Amino Acids

44.8

Weight (kDa)

8.49

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 128 - 396 1.7e-26 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 815
AccBSI CCGCTC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 6 cut(s) 250, 297, 416, 588, 1096, 1208
AfiI CCNNNNNNNGG 3 cut(s) 332, 980, 1038
AgsI TTSAA 5 cut(s) 63, 81, 487, 566, 642
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 2 cut(s) 352, 717
AluBI AGCT 4 cut(s) 184, 430, 784, 1044
AluI AGCT 4 cut(s) 184, 430, 784, 1044
Alw21I GWGCWC 2 cut(s) 375, 432
Alw26I GTCTC 2 cut(s) 48, 1159
Alw44I GTGCAC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 933
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 2 cut(s) 133, 232
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 4 cut(s) 184, 228, 237, 1028
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 3 cut(s) 342, 403, 617
AsuII TTCGAA 2 cut(s) 711, 1072
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 1 cut(s) 432
BauI CACGAG 1 cut(s) 663
Bbv12I GWGCWC 2 cut(s) 375, 432
BbvCI CCTCAGC 1 cut(s) 647
BbvI GCAGC 4 cut(s) 171, 215, 224, 1040
BccI CCATC 1 cut(s) 951
BceAI ACGGC 1 cut(s) 640
BciT130I CCWGG 2 cut(s) 354, 719
BciVI GTATCC 1 cut(s) 669
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 48, 1159
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 2 cut(s) 146, 1133
BfuI GTATCC 1 cut(s) 669
BisI GCNGC 6 cut(s) 185, 229, 232, 235, 238, 1029
BlsI GCNGC 6 cut(s) 186, 230, 233, 236, 239, 1030
Bme1390I CCNGG 3 cut(s) 267, 354, 719
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 2 cut(s) 817, 1173
BmrFI CCNGG 3 cut(s) 267, 354, 719
BmrI ACTGGG 1 cut(s) 14
BmsI GCATC 2 cut(s) 450, 890
BmuI ACTGGG 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 647
Bpu14I TTCGAA 2 cut(s) 711, 1072
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 717, 718, 833
BsaXI ACNNNNNCTCC 4 cut(s) 56, 85, 86, 115
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 980, 1038
Bse118I RCCGGY 2 cut(s) 332, 1121
Bse1I ACTGG 5 cut(s) 7, 20, 246, 828, 1102
Bse8I GATNNNNATC 1 cut(s) 681
BseBI CCWGG 2 cut(s) 354, 719
BseDI CCNNGG 3 cut(s) 717, 718, 833
BseGI GGATG 4 cut(s) 218, 599, 1002, 1207
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 3 cut(s) 332, 980, 1038
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 5 cut(s) 7, 20, 246, 828, 1102
BseRI GAGGAG 2 cut(s) 90, 461
BseSI GKGCMC 1 cut(s) 375
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 4 cut(s) 171, 215, 224, 1040
BseYI CCCAGC 1 cut(s) 973
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 135, 234
BshNI GGYRCC 1 cut(s) 815
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 375, 432
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 3 cut(s) 266, 333, 1122
BslFI GGGAC 3 cut(s) 293, 684, 887
BslI CCNNNNNNNGG 3 cut(s) 332, 980, 1038
BsmAI GTCTC 2 cut(s) 48, 1159
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 293, 684, 887
BsnI GGCC 2 cut(s) 135, 234
BsoBI CYCGRG 1 cut(s) 418
Bsp119I TTCGAA 2 cut(s) 711, 1072
Bsp1286I GDGCHC 2 cut(s) 375, 432
Bsp1407I TGTACA 1 cut(s) 1206
Bsp143I GATC 2 cut(s) 347, 393
Bsp19I CCATGG 1 cut(s) 833
BspANI GGCC 2 cut(s) 135, 234
BspCNI CTCAG 1 cut(s) 639
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 3 cut(s) 474, 685, 987
BspLI GGNNCC 2 cut(s) 817, 1173
BspT104I TTCGAA 2 cut(s) 711, 1072
BspT107I GGYRCC 1 cut(s) 815
BsrBI CCGCTC 1 cut(s) 279
BsrFI RCCGGY 2 cut(s) 332, 1121
BsrGI TGTACA 1 cut(s) 1206
BsrI ACTGG 5 cut(s) 7, 20, 246, 828, 1102
BssAI RCCGGY 2 cut(s) 332, 1121
BssECI CCNNGG 3 cut(s) 717, 718, 833
BssMI GATC 2 cut(s) 347, 393
BssNI GRCGYC 1 cut(s) 450
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 833
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 354, 719
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 450
BstAUI TGTACA 1 cut(s) 1206
BstBI TTCGAA 2 cut(s) 711, 1072
BstC8I GCNNGC 7 cut(s) 91, 334, 512, 1123, 1127, 1138, 1189
BstDEI CTNAG 1 cut(s) 647
BstDSI CCRYGG 1 cut(s) 833
BstF5I GGATG 4 cut(s) 218, 599, 1002, 1207
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 2 cut(s) 350, 396
BstMAI GTCTC 2 cut(s) 48, 1159
BstMBI GATC 2 cut(s) 347, 393
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 5 cut(s) 234, 237, 276, 370, 652
BstNI CCWGG 2 cut(s) 354, 719
BstNSI RCATGY 4 cut(s) 93, 463, 514, 1129
BstSCI CCNGG 3 cut(s) 265, 352, 717
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 171, 215, 224, 1040
BstZI CGGCCG 1 cut(s) 232
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 2 cut(s) 135, 234
BtgI CCRYGG 1 cut(s) 833
BtsCI GGATG 4 cut(s) 218, 599, 1002, 1207
BtsIMutI CAGTG 2 cut(s) 516, 934
Cac8I GCNNGC 7 cut(s) 91, 334, 512, 1123, 1127, 1138, 1189
CaiI CAGNNNCTG 1 cut(s) 933
CciI TCATGA 3 cut(s) 474, 685, 987
CciNI GCGGCCGC 1 cut(s) 232
Cfr10I RCCGGY 2 cut(s) 332, 1121
Cfr13I GGNCC 1 cut(s) 356
CseI GACGC 2 cut(s) 244, 458
Csp6I GTAC 6 cut(s) 249, 296, 415, 587, 1095, 1207
CviQI GTAC 6 cut(s) 249, 296, 415, 587, 1095, 1207
DdeI CTNAG 1 cut(s) 647
DpnI GATC 2 cut(s) 349, 395
DpnII GATC 2 cut(s) 347, 393
EaeI YGGCCR 1 cut(s) 232
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 2 cut(s) 351, 1155
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 833
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 2 cut(s) 405, 683
Eco47I GGWCC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 352, 717
EcoRV GATATC 2 cut(s) 405, 683
EcoT14I CCWWGG 1 cut(s) 833
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 833
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 3 cut(s) 293, 684, 887
FauI CCCGC 3 cut(s) 260, 284, 392
FauNDI CATATG 1 cut(s) 1036
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 6 cut(s) 185, 229, 232, 235, 238, 1029
FokI GGATG 4 cut(s) 205, 606, 989, 1214
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 6 cut(s) 185, 229, 232, 235, 238, 1029
FspBI CTAG 2 cut(s) 146, 1133
GluI GCNGC 6 cut(s) 185, 229, 232, 235, 238, 1029
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 2 cut(s) 135, 234
HapII CCGG 3 cut(s) 266, 333, 1122
HgaI GACGC 2 cut(s) 244, 458
Hin1I GRCGYC 1 cut(s) 450
HincII GTYRAC 3 cut(s) 154, 599, 940
HindII GTYRAC 3 cut(s) 154, 599, 940
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 107, 196, 500, 544, 608
HpaII CCGG 3 cut(s) 266, 333, 1122
HphI GGTGA 3 cut(s) 342, 403, 617
Hpy166II GTNNAC 8 cut(s) 154, 373, 599, 940, 1022, 1160, 1207, 1209
Hpy188I TCNGA 1 cut(s) 845
Hpy188III TCNNGA 7 cut(s) 104, 475, 494, 665, 686, 988, 1015
Hpy8I GTNNAC 8 cut(s) 154, 373, 599, 940, 1022, 1160, 1207, 1209
Hpy99I CGWCG 2 cut(s) 215, 260
HpyAV CCTTC 3 cut(s) 198, 379, 623
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 369, 577
HpyCH4V TGCA 7 cut(s) 4, 89, 93, 373, 510, 749, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 234, 237, 276, 370, 652
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 2 cut(s) 369, 577
Hsp92I GRCGYC 1 cut(s) 450
KroI GCCGGC 2 cut(s) 332, 1121
KroNI GCCGGC 2 cut(s) 334, 1123
Kzo9I GATC 2 cut(s) 347, 393
LmnI GCTCC 3 cut(s) 284, 1041, 1171
Lsp1109I GCAGC 4 cut(s) 171, 215, 224, 1040
LweI GCATC 2 cut(s) 450, 890
MaeI CTAG 2 cut(s) 146, 1133
MaeII ACGT 2 cut(s) 369, 577
MaeIII GTNAC 3 cut(s) 54, 605, 1108
MalI GATC 2 cut(s) 349, 395
MbiI CCGCTC 1 cut(s) 279
MboI GATC 2 cut(s) 347, 393
MboII GAAGA 6 cut(s) 394, 509, 533, 536, 683, 686
MfeI CAATTG 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 375, 432
MluCI AATT 6 cut(s) 124, 572, 753, 765, 792, 1178
MlyI GAGTC 2 cut(s) 205, 602
MmeI TCCRAC 1 cut(s) 1179
MroNI GCCGGC 2 cut(s) 332, 1121
MseI TTAA 2 cut(s) 759, 1181
MslI CAYNNNNRTG 2 cut(s) 116, 509
MspA1I CMGCKG 3 cut(s) 184, 228, 237
MspI CCGG 3 cut(s) 266, 333, 1122
MspR9I CCNGG 3 cut(s) 267, 354, 719
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 2 cut(s) 354, 719
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 234, 237, 276, 370, 652
NaeI GCCGGC 2 cut(s) 334, 1123
NciI CCSGG 1 cut(s) 267
NcoI CCATGG 1 cut(s) 833
NdeI CATATG 1 cut(s) 1036
NdeII GATC 2 cut(s) 347, 393
NgoMIV GCCGGC 2 cut(s) 332, 1121
NlaIV GGNNCC 2 cut(s) 817, 1173
NmuCI GTSAC 1 cut(s) 605
NotI GCGGCCGC 1 cut(s) 232
NspI RCATGY 4 cut(s) 93, 463, 514, 1129
NspV TTCGAA 2 cut(s) 711, 1072
PaeI GCATGC 3 cut(s) 93, 514, 1129
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 3 cut(s) 474, 685, 987
PasI CCCWGGG 1 cut(s) 718
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 2 cut(s) 334, 1123
PfeI GAWTC 3 cut(s) 107, 500, 544
PkrI GCNGC 6 cut(s) 186, 230, 233, 236, 239, 1030
PleI GAGTC 2 cut(s) 204, 602
PpsI GAGTC 2 cut(s) 204, 602
PpuMI RGGWCCY 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 2 cut(s) 352, 717
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 2 cut(s) 352, 717
PspN4I GGNNCC 2 cut(s) 817, 1173
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PspXI VCTCGAGB 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 933
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 6 cut(s) 250, 297, 416, 588, 1096, 1208
RsaNI GTAC 6 cut(s) 249, 296, 415, 587, 1095, 1207
RseI CAYNNNNRTG 2 cut(s) 116, 509
SacI GAGCTC 1 cut(s) 432
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 759, 1181
SatI GCNGC 6 cut(s) 185, 229, 232, 235, 238, 1029
Sau3AI GATC 2 cut(s) 347, 393
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 2 cut(s) 205, 602
ScrFI CCNGG 3 cut(s) 267, 354, 719
SduI GDGCHC 2 cut(s) 375, 432
SfaNI GCATC 2 cut(s) 450, 890
Sfr274I CTCGAG 1 cut(s) 418
SfuI TTCGAA 2 cut(s) 711, 1072
SinI GGWCC 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 116, 509
SmlI CTYRAG 2 cut(s) 418, 704
SmoI CTYRAG 2 cut(s) 418, 704
SpeI ACTAGT 1 cut(s) 145
SphI GCATGC 3 cut(s) 93, 514, 1129
Sse9I AATT 6 cut(s) 124, 572, 753, 765, 792, 1178
SspMI CTAG 2 cut(s) 146, 1133
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 265, 352, 717
StyI CCWWGG 1 cut(s) 833
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 372, 580
TaqI TCGA 4 cut(s) 153, 419, 711, 1072
TasI AATT 6 cut(s) 124, 572, 753, 765, 792, 1178
TatI WGTACW 1 cut(s) 1206
TauI GCSGC 2 cut(s) 234, 237
TfiI GAWTC 3 cut(s) 107, 500, 544
Tru1I TTAA 2 cut(s) 759, 1181
Tru9I TTAA 2 cut(s) 759, 1181
TscAI CASTG 2 cut(s) 523, 934
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 4 cut(s) 184, 228, 237, 1028
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 4 cut(s) 684, 702, 776, 991
TspGWI ACGGA 2 cut(s) 211, 1177
TspRI CASTG 2 cut(s) 523, 934
VneI GTGCAC 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 356
XceI RCATGY 4 cut(s) 93, 463, 514, 1129
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 2 cut(s) 146, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.