Rw1G015870

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000588
Physical Location & Seq
Reverse (-)
35444359 .. 35446174
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015870.1

Sequence Viewer

Length: 1146 bp
ATGAGCATTTATGGAGGCCAACGACAACTGGTGGTCGACTGTTGCTATTGCCAGACCCAAATCCAGCTGCCTTCTGTGAATCCGATATACCTCGACGGCACATCCGTCCGCTGCGGCCGCTGCTATGCCAGTACCCAAGTCGCCTCTCCTGGCTCCACCCGCTCCCTCCATAGTGCTGTAGCCTACTATGTCGACGTCTTATTTTCAGGTTGCACTATTGTTTCTGGTAATAAAGTTAGTGGAAACACTGGGAACCATAATGGAAGTTTTTCTGTTGGCAACACTCTCCCTCCCTCGCCCTACAACCACGCGCCGCCTGGACCTCCGCCGAACGTGCACGGCCGGAAGAAGGCGGTGATCTGCGGGATATCGTATAAATACTCGAGGCATGAGCTCAAGGGTTGCATCAATGACGCCAAATGCATGCGGCATCTCCTCATCAACAAATTCAAGTTTCCAGATGATTCCATTCTCATGCTCACTGAAGAAGAAACTCAGCCAGCTAAGATTCCAAATAGATACAACATTAGAATGGCATTACATTGGCTTGTACAAGGATGCCAACCAGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGGCAGAGGGATTATCATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTTGAAACCCAGGGTATGATTGTCGATGATGAGATAAATGCAACAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGACTCTGGTCATAGTGGCACTGTACTGGATTTGCCATTCCTTTGCAGAATGAACAGGGGTGGAAAATATGTATGGGAGGATCATCGCCCTAGATCAGGCATATGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGACGATCAAACCTCTGCTGATACATCAGCTCTATCCAAGATCACATCAACCAGTGCCATGACTTTCTGCTTCATCCAAGCAATCGAGCGCGGACAGGCAGCCACATATGGAAGCATACTCAATTCTATGCGTAATATCATTCGAAGTACGGGCTCTGGTGGTGGTAGGCTAAGACAGGAGCCTCAATTAACTTCCTGTGAGCCGTTCGATGTGTACACGAAACCTTTCGCCCTATGA

Protein Analysis

381

Amino Acids

41.68

Weight (kDa)

7.18

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 115 - 370 1e-34 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 2 cut(s) 36, 192
AccII CGCG 2 cut(s) 311, 999
AclWI GGATC 1 cut(s) 846
AcoI YGGCCR 2 cut(s) 115, 340
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 504
AcyI GRCGYC 2 cut(s) 195, 414
AfaI GTAC 5 cut(s) 133, 552, 783, 1057, 1124
AfiI CCNNNNNNNGG 2 cut(s) 349, 854
AgsI TTSAA 2 cut(s) 451, 674
AjnI CCWGG 4 cut(s) 148, 316, 565, 678
AluBI AGCT 5 cut(s) 67, 394, 503, 745, 938
AluI AGCT 5 cut(s) 67, 394, 503, 745, 938
Alw21I GWGCWC 2 cut(s) 339, 396
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 1 cut(s) 846
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 4 cut(s) 16, 115, 340, 722
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 67, 111, 120, 1007
ApoI RAATTY 1 cut(s) 446
Asp700I GAANNNNTTC 2 cut(s) 268, 1133
AspLEI GCGC 2 cut(s) 313, 999
AspS9I GGNCC 2 cut(s) 320, 723
AsuHPI GGTGA 3 cut(s) 367, 581, 638
AsuII TTCGAA 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 382
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 1 cut(s) 339
BanII GRGCYC 2 cut(s) 396, 1064
Bbv12I GWGCWC 2 cut(s) 339, 396
BbvI GCAGC 4 cut(s) 54, 98, 107, 1019
BccI CCATC 1 cut(s) 632
BceAI ACGGC 4 cut(s) 112, 355, 623, 1096
BciT130I CCWGG 4 cut(s) 150, 318, 567, 680
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 1 cut(s) 177
BisI GCNGC 8 cut(s) 68, 112, 115, 118, 121, 314, 428, 1008
BlsI GCNGC 8 cut(s) 69, 113, 116, 119, 122, 315, 429, 1009
Bme1390I CCNGG 4 cut(s) 150, 318, 567, 680
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 320, 723
BmiI GGNNCC 3 cut(s) 154, 254, 1089
BmrFI CCNGG 4 cut(s) 150, 318, 567, 680
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 3 cut(s) 414, 439, 548
BmuI ACTGGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 765
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 380
BsaBI GATNNNNATC 1 cut(s) 912
BsaHI GRCGYC 2 cut(s) 195, 414
BsaJI CCNNGG 2 cut(s) 678, 679
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 854
Bse1I ACTGG 5 cut(s) 33, 129, 253, 789, 960
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 4 cut(s) 150, 318, 567, 680
BseDI CCNNGG 2 cut(s) 678, 679
BseGI GGATG 3 cut(s) 101, 563, 981
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 2 cut(s) 349, 854
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 5 cut(s) 33, 129, 253, 789, 960
BseRI GAGGAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 339
BseX3I CGGCCG 2 cut(s) 115, 340
BseXI GCAGC 4 cut(s) 54, 98, 107, 1019
Bsh1236I CGCG 2 cut(s) 311, 999
Bsh1285I CGRYCG 2 cut(s) 118, 343
BshFI GGCC 4 cut(s) 18, 117, 342, 724
BsiEI CGRYCG 2 cut(s) 118, 343
BsiHKAI GWGCWC 2 cut(s) 339, 396
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 1 cut(s) 645
BslI CCNNNNNNNGG 2 cut(s) 349, 854
BsmFI GGGAC 1 cut(s) 645
BsnI GGCC 4 cut(s) 18, 117, 342, 724
BsoBI CYCGRG 1 cut(s) 382
Bsp119I TTCGAA 1 cut(s) 1051
Bsp1286I GDGCHC 3 cut(s) 339, 396, 1064
Bsp1407I TGTACA 2 cut(s) 550, 1122
Bsp143I GATC 5 cut(s) 357, 838, 851, 913, 948
BspANI GGCC 4 cut(s) 18, 117, 342, 724
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 2 cut(s) 311, 999
BspLI GGNNCC 3 cut(s) 154, 254, 1089
BspPI GGATC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 1051
BsrBI CCGCTC 1 cut(s) 162
BsrGI TGTACA 2 cut(s) 550, 1122
BsrI ACTGG 5 cut(s) 33, 129, 253, 789, 960
BssECI CCNNGG 2 cut(s) 678, 679
BssMI GATC 5 cut(s) 357, 838, 851, 913, 948
BssNI GRCGYC 2 cut(s) 195, 414
Bst2UI CCWGG 4 cut(s) 150, 318, 567, 680
Bst4CI ACNGT 2 cut(s) 41, 781
BstACI GRCGYC 2 cut(s) 195, 414
BstAUI TGTACA 2 cut(s) 550, 1122
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 2 cut(s) 425, 501
BstDEI CTNAG 3 cut(s) 495, 504, 1079
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 3 cut(s) 101, 563, 981
BstFNI CGCG 2 cut(s) 311, 999
BstHHI GCGC 2 cut(s) 313, 999
BstKTI GATC 5 cut(s) 360, 841, 854, 916, 951
BstMBI GATC 5 cut(s) 357, 838, 851, 913, 948
BstMCI CGRYCG 2 cut(s) 118, 343
BstMWI GCNNNNNNNGC 4 cut(s) 117, 120, 159, 334
BstNI CCWGG 4 cut(s) 150, 318, 567, 680
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 765
BstSCI CCNGG 4 cut(s) 148, 316, 565, 678
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 2 cut(s) 311, 999
BstV1I GCAGC 4 cut(s) 54, 98, 107, 1019
BstZI CGGCCG 2 cut(s) 115, 340
BsuRI GGCC 4 cut(s) 18, 117, 342, 724
BtgZI GCGATG 1 cut(s) 827
BtsCI GGATG 3 cut(s) 101, 563, 981
BtsIMutI CAGTG 5 cut(s) 246, 480, 777, 904, 967
Cac8I GCNNGC 2 cut(s) 425, 501
CciNI GCGGCCGC 1 cut(s) 115
CfoI GCGC 2 cut(s) 313, 999
Cfr13I GGNCC 2 cut(s) 320, 723
CseI GACGC 1 cut(s) 422
CsiI ACCWGGT 1 cut(s) 565
Csp6I GTAC 5 cut(s) 132, 551, 782, 1056, 1123
CviAII CATG 8 cut(s) 389, 424, 475, 599, 623, 734, 749, 967
CviQI GTAC 5 cut(s) 132, 551, 782, 1056, 1123
DdeI CTNAG 3 cut(s) 495, 504, 1079
DpnI GATC 5 cut(s) 359, 840, 853, 915, 950
DpnII GATC 5 cut(s) 357, 838, 851, 913, 948
EaeI YGGCCR 2 cut(s) 115, 340
EagI CGGCCG 2 cut(s) 115, 340
EciI GGCGGA 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 394
EclXI CGGCCG 2 cut(s) 115, 340
Eco24I GRGCYC 2 cut(s) 396, 1064
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 320
Eco52I CGGCCG 2 cut(s) 115, 340
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRII CCWGG 4 cut(s) 148, 316, 565, 678
EcoRV GATATC 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 2 cut(s) 396, 1064
FaeI CATG 8 cut(s) 392, 427, 478, 602, 626, 737, 752, 970
FaqI GGGAC 1 cut(s) 645
FatI CATG 8 cut(s) 388, 423, 474, 598, 622, 733, 748, 966
FauI CCCGC 2 cut(s) 167, 356
FauNDI CATATG 2 cut(s) 860, 1015
FblI GTMKAC 2 cut(s) 36, 192
Fnu4HI GCNGC 8 cut(s) 68, 112, 115, 118, 121, 314, 428, 1008
FokI GGATG 3 cut(s) 88, 570, 968
FriOI GRGCYC 2 cut(s) 396, 1064
Fsp4HI GCNGC 8 cut(s) 68, 112, 115, 118, 121, 314, 428, 1008
FspBI CTAG 1 cut(s) 849
GlaI GCGC 2 cut(s) 312, 998
GluI GCNGC 8 cut(s) 68, 112, 115, 118, 121, 314, 428, 1008
HaeIII GGCC 4 cut(s) 18, 117, 342, 724
HapII CCGG 1 cut(s) 343
HgaI GACGC 1 cut(s) 422
HhaI GCGC 2 cut(s) 313, 999
Hin1I GRCGYC 2 cut(s) 195, 414
Hin1II CATG 8 cut(s) 392, 427, 478, 602, 626, 737, 752, 970
Hin6I GCGC 2 cut(s) 311, 997
HinP1I GCGC 2 cut(s) 311, 997
HincII GTYRAC 2 cut(s) 37, 193
HindII GTYRAC 2 cut(s) 37, 193
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 5 cut(s) 79, 464, 508, 572, 761
HpaII CCGG 1 cut(s) 343
HphI GGTGA 3 cut(s) 367, 581, 638
Hpy166II GTNNAC 6 cut(s) 37, 193, 337, 605, 1123, 1125
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 1 cut(s) 458
Hpy8I GTNNAC 6 cut(s) 37, 193, 337, 605, 1123, 1125
Hpy99I CGWCG 2 cut(s) 98, 197
HpyAV CCTTC 2 cut(s) 81, 343
HpyCH4III ACNGT 2 cut(s) 41, 781
HpyCH4IV ACGT 2 cut(s) 195, 333
HpyCH4V TGCA 7 cut(s) 213, 337, 405, 423, 710, 752, 804
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 120, 159, 334
HpyF3I CTNAG 3 cut(s) 495, 504, 1079
HpySE526I ACGT 2 cut(s) 195, 333
Hsp92I GRCGYC 2 cut(s) 195, 414
Hsp92II CATG 8 cut(s) 392, 427, 478, 602, 626, 737, 752, 970
HspAI GCGC 2 cut(s) 311, 997
Kzo9I GATC 5 cut(s) 357, 838, 851, 913, 948
LmnI GCTCC 3 cut(s) 158, 167, 1087
Lsp1109I GCAGC 4 cut(s) 54, 98, 107, 1019
LweI GCATC 3 cut(s) 414, 439, 548
MabI ACCWGGT 1 cut(s) 565
MaeI CTAG 1 cut(s) 849
MaeII ACGT 2 cut(s) 195, 333
MaeIII GTNAC 1 cut(s) 569
MalI GATC 5 cut(s) 359, 840, 853, 915, 950
MbiI CCGCTC 1 cut(s) 162
MboI GATC 5 cut(s) 357, 838, 851, 913, 948
MboII GAAGA 3 cut(s) 358, 497, 500
MfeI CAATTG 1 cut(s) 714
MhlI GDGCHC 3 cut(s) 339, 396, 1064
MluCI AATT 5 cut(s) 446, 714, 753, 1030, 1094
MlyI GAGTC 2 cut(s) 566, 755
Mph1103I ATGCAT 1 cut(s) 425
MroXI GAANNNNTTC 2 cut(s) 268, 1133
MseI TTAA 1 cut(s) 1097
MslI CAYNNNNRTG 3 cut(s) 473, 530, 627
MspA1I CMGCKG 3 cut(s) 67, 111, 120
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 4 cut(s) 150, 318, 567, 680
MunI CAATTG 1 cut(s) 714
MvaI CCWGG 4 cut(s) 150, 318, 567, 680
MvnI CGCG 2 cut(s) 311, 999
MwoI GCNNNNNNNGC 4 cut(s) 117, 120, 159, 334
NdeI CATATG 2 cut(s) 860, 1015
NdeII GATC 5 cut(s) 357, 838, 851, 913, 948
NlaIII CATG 8 cut(s) 392, 427, 478, 602, 626, 737, 752, 970
NlaIV GGNNCC 3 cut(s) 154, 254, 1089
NmuCI GTSAC 1 cut(s) 569
NotI GCGGCCGC 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 1 cut(s) 427
NspV TTCGAA 1 cut(s) 1051
PaeI GCATGC 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 2 cut(s) 268, 1133
PfeI GAWTC 3 cut(s) 79, 464, 508
PkrI GCNGC 8 cut(s) 69, 113, 116, 119, 122, 315, 429, 1009
PleI GAGTC 2 cut(s) 566, 755
PpsI GAGTC 2 cut(s) 566, 755
PshAI GACNNNNGTC 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 4 cut(s) 148, 316, 565, 678
PspGI CCWGG 4 cut(s) 148, 316, 565, 678
PspN4I GGNNCC 3 cut(s) 154, 254, 1089
PspPI GGNCC 2 cut(s) 320, 723
PspXI VCTCGAGB 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 67
RsaI GTAC 5 cut(s) 133, 552, 783, 1057, 1124
RsaNI GTAC 5 cut(s) 132, 551, 782, 1056, 1123
RseI CAYNNNNRTG 3 cut(s) 473, 530, 627
SacI GAGCTC 1 cut(s) 396
SalI GTCGAC 2 cut(s) 35, 191
SaqAI TTAA 1 cut(s) 1097
SatI GCNGC 8 cut(s) 68, 112, 115, 118, 121, 314, 428, 1008
Sau3AI GATC 5 cut(s) 357, 838, 851, 913, 948
Sau96I GGNCC 2 cut(s) 320, 723
SchI GAGTC 2 cut(s) 566, 755
ScrFI CCNGG 4 cut(s) 150, 318, 567, 680
SduI GDGCHC 3 cut(s) 339, 396, 1064
SexAI ACCWGGT 1 cut(s) 565
SfaNI GCATC 3 cut(s) 414, 439, 548
SfcI CTRYAG 1 cut(s) 177
Sfr274I CTCGAG 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 1051
SinI GGWCC 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 382
SmiMI CAYNNNNRTG 3 cut(s) 473, 530, 627
SmlI CTYRAG 2 cut(s) 382, 395
SmoI CTYRAG 2 cut(s) 382, 395
SphI GCATGC 1 cut(s) 427
Sse9I AATT 5 cut(s) 446, 714, 753, 1030, 1094
SspMI CTAG 1 cut(s) 849
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 148, 316, 565, 678
TaaI ACNGT 2 cut(s) 41, 781
TaiI ACGT 2 cut(s) 198, 336
TaqI TCGA 8 cut(s) 36, 93, 192, 383, 693, 993, 1051, 1116
TasI AATT 5 cut(s) 446, 714, 753, 1030, 1094
TatI WGTACW 3 cut(s) 550, 781, 1122
TauI GCSGC 4 cut(s) 117, 120, 316, 430
TfiI GAWTC 3 cut(s) 79, 464, 508
Tru1I TTAA 1 cut(s) 1097
Tru9I TTAA 1 cut(s) 1097
TscAI CASTG 5 cut(s) 253, 487, 784, 904, 967
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 4 cut(s) 67, 111, 120, 1007
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 5 cut(s) 645, 663, 737, 824, 970
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 5 cut(s) 253, 487, 784, 904, 967
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 382
XmiI GTMKAC 2 cut(s) 36, 192
XmnI GAANNNNTTC 2 cut(s) 268, 1133
XspI CTAG 1 cut(s) 849
ZraI GACGTC 1 cut(s) 196
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.