MD02G1221900.v1.1

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000588
Physical Location & Seq
Forward (+)
25873390 .. 25877448
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1221900.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTATAACATGCTGGTGAACTGCGCCCACTGCCGCACCCCTCTCCAGATTCCCCCCGGCGCAGAGTCCATCCGCTGCGCCATCTGCCAGGGTGTCACTCTAATCGCCGACCCACGAGGCCTCCCTCAGCCTCCAGCCTCCCACGCGCCTCCACCTCCCGCTCCATATTCTCACGCGCCTCCACCTCCCGCTCCGTACTCTCACGCGCCTCCAGGCCCTCCCCCCAACGCGCACGGGCGGAAGAAGGCCGTGATCTGCGGGATCTCGTATAAGTACTCGAGGCATGAGCTCAAGGGTTGCATCAATGATGCCAAGTGCATGCGGTATCTCCTCATCAACAAGTTCCAGTTTCCAGAAGATTGCATTGTCATGCTCACTGAAGAAGAAACTCACCCCTCCAAGATTCCAAACAAATATAACATTAGAATGGCATTATATTGGCTTGTACAAGGCTGTCAAGCAGGCGACTCCCTCTTTTTTCATTACTCGGGCCATGGTTCACGGCAGAGGAATTATAATGGCGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTTGAAACTCAGGGTATGATTGTTGATGATGAGATAAATGCAGCAATTGTGAGACCCATTCCACCCGGGGCTAAGCTTCATGCAATAATAGATGCTTGTCATAGTGGCACTGTACTGGATTTGCCATTCCTTTGCAGAATGGACAGGAGTGGACGATATGTATGGGAGGATCATCGCCCTCGATCAGGAATGTGGAAAGGATCAGGCGGTGGAGAAGTCATTTCCTTCAGTGGTTGTGATGATGATCAAACGTCTGCTGACACATCAGCTCTATCAAAGATCACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCAATTGAGCGTGGACAGGCGGGCACCTATGGAAGCATACTCAATTCTATGCGCTCTACCATTCGGAGTACAGGTACGGGTGGTGGTGGTGGTGGTGGTGGCAGTTTAACATCTCTTCTTGGTGGTGGTGGTGGTGGTGGTGGTGTGACATCCCTGGTCAGCATGCTTCTTACAGGAGGCAGTGATACTGGTGGGCTAAGACAGGAACCGCAATTAACTGCCTGTGAGCCATTTGATGTGTATGCAAAACCCTTCTCCCTATGA

Protein Analysis

382

Amino Acids

40.63

Weight (kDa)

6.3

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LSD1 PF06943 8 - 32 3.9e-09 LSD1 zinc finger
Peptidase_C14 PF00656 80 - 317 1.4e-33 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AccB1I GGYRCC 2 cut(s) 857, 905
AccBSI CCGCTC 2 cut(s) 161, 191
AccII CGCG 4 cut(s) 146, 176, 206, 230
AclWI GGATC 3 cut(s) 269, 741, 772
AcuI CTGAAG 2 cut(s) 399, 775
AfaI GTAC 6 cut(s) 197, 275, 447, 678, 952, 958
AfiI CCNNNNNNNGG 1 cut(s) 749
AgsI TTSAA 1 cut(s) 569
AjnI CCWGG 3 cut(s) 87, 211, 1035
AluBI AGCT 3 cut(s) 289, 640, 833
AluI AGCT 3 cut(s) 289, 640, 833
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 610
AlwI GGATC 3 cut(s) 269, 741, 772
Ama87I CYCGRG 3 cut(s) 277, 487, 629
AoxI GGCC 4 cut(s) 118, 214, 246, 490
ApeKI GCWGC 2 cut(s) 75, 605
AspLEI GCGC 8 cut(s) 26, 62, 80, 148, 178, 208, 232, 936
AspS9I GGNCC 2 cut(s) 215, 490
AsuC2I CCSGG 3 cut(s) 57, 630, 631
AsuHPI GGTGA 2 cut(s) 28, 383
AvaI CYCGRG 3 cut(s) 277, 487, 629
BaeGI GKGCMC 1 cut(s) 908
BanI GGYRCC 2 cut(s) 857, 905
BanII GRGCYC 1 cut(s) 291
BauI CACGAG 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 62, 617
BccI CCATC 3 cut(s) 77, 89, 527
BceAI ACGGC 2 cut(s) 233, 518
BciT130I CCWGG 3 cut(s) 89, 213, 1037
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 3 cut(s) 57, 630, 631
BcoDI GTCTC 1 cut(s) 610
BisI GCNGC 3 cut(s) 34, 76, 606
BlpI GCTNAGC 1 cut(s) 636
BlsI GCNGC 3 cut(s) 35, 77, 607
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 6 cut(s) 57, 89, 213, 630, 631, 1037
BmeT110I CYCGRG 3 cut(s) 277, 487, 629
BmgT120I GGNCC 2 cut(s) 215, 490
BmiI GGNNCC 3 cut(s) 859, 907, 1089
BmrFI CCNGG 6 cut(s) 57, 89, 213, 630, 631, 1037
BmsI GCATC 3 cut(s) 298, 309, 646
BpmI CTGGAG 3 cut(s) 29, 117, 195
Bpu10I CCTNAGC 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 3 cut(s) 57, 630, 631
BsaBI GATNNNNATC 1 cut(s) 807
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 6 cut(s) 55, 88, 493, 629, 630, 1035
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 940, 970
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse1I ACTGG 3 cut(s) 346, 684, 1075
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 3 cut(s) 89, 213, 1037
BseDI CCNNGG 6 cut(s) 55, 88, 493, 629, 630, 1035
BseGI GGATG 3 cut(s) 69, 876, 1031
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMII CTCAG 2 cut(s) 140, 587
BseNI ACTGG 3 cut(s) 346, 684, 1075
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 908
BseXI GCAGC 2 cut(s) 62, 617
Bsh1236I CGCG 4 cut(s) 146, 176, 206, 230
BshFI GGCC 4 cut(s) 120, 216, 248, 492
BshNI GGYRCC 2 cut(s) 857, 905
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 3 cut(s) 277, 487, 629
BsiSI CCGG 2 cut(s) 57, 630
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 1 cut(s) 610
BsmFI GGGAC 1 cut(s) 540
BsnI GGCC 4 cut(s) 120, 216, 248, 492
Bso31I GGTCTC 1 cut(s) 610
BsoBI CYCGRG 3 cut(s) 277, 487, 629
Bsp1286I GDGCHC 2 cut(s) 291, 908
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
Bsp1720I GCTNAGC 1 cut(s) 636
Bsp19I CCATGG 1 cut(s) 493
BspANI GGCC 4 cut(s) 120, 216, 248, 492
BspCNI CTCAG 2 cut(s) 139, 586
BspFNI CGCG 4 cut(s) 146, 176, 206, 230
BspLI GGNNCC 3 cut(s) 859, 907, 1089
BspPI GGATC 3 cut(s) 269, 741, 772
BspT107I GGYRCC 2 cut(s) 857, 905
BspTNI GGTCTC 1 cut(s) 610
BsrBI CCGCTC 2 cut(s) 161, 191
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 3 cut(s) 346, 684, 1075
BssECI CCNNGG 6 cut(s) 55, 88, 493, 629, 630, 1035
BssMI GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 3 cut(s) 89, 213, 1037
Bst4CI ACNGT 1 cut(s) 676
Bst6I CTCTTC 1 cut(s) 1002
BstAUI TGTACA 1 cut(s) 445
BstC8I GCNNGC 4 cut(s) 320, 463, 904, 1046
BstDEI CTNAG 4 cut(s) 126, 573, 636, 1079
BstDSI CCRYGG 1 cut(s) 493
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 69, 876, 1031
BstFNI CGCG 4 cut(s) 146, 176, 206, 230
BstHHI GCGC 8 cut(s) 26, 62, 80, 148, 178, 208, 232, 936
BstKTI GATC 7 cut(s) 255, 264, 736, 749, 767, 811, 846
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
BstMWI GCNNNNNNNGC 3 cut(s) 30, 84, 143
BstNI CCWGG 3 cut(s) 89, 213, 1037
BstNSI RCATGY 3 cut(s) 13, 322, 1048
BstSCI CCNGG 6 cut(s) 55, 87, 211, 628, 629, 1035
BstSLI GKGCMC 1 cut(s) 908
BstUI CGCG 4 cut(s) 146, 176, 206, 230
BstV1I GCAGC 2 cut(s) 62, 617
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 4 cut(s) 120, 216, 248, 492
BtgI CCRYGG 1 cut(s) 493
BtgZI GCGATG 2 cut(s) 537, 722
BtsCI GGATG 3 cut(s) 69, 876, 1031
BtsI GCAGTG 2 cut(s) 28, 1069
BtsIMutI CAGTG 5 cut(s) 28, 375, 672, 799, 1069
Cac8I GCNNGC 4 cut(s) 320, 463, 904, 1046
CfoI GCGC 8 cut(s) 26, 62, 80, 148, 178, 208, 232, 936
Cfr13I GGNCC 2 cut(s) 215, 490
Cfr9I CCCGGG 1 cut(s) 629
Csp6I GTAC 6 cut(s) 196, 274, 446, 677, 951, 957
CviAII CATG 8 cut(s) 10, 284, 319, 370, 494, 644, 862, 1045
CviQI GTAC 6 cut(s) 196, 274, 446, 677, 951, 957
DdeI CTNAG 4 cut(s) 126, 573, 636, 1079
DpnI GATC 7 cut(s) 254, 263, 735, 748, 766, 810, 845
DpnII GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
EciI GGCGGA 1 cut(s) 253
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 493
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 399, 775
Eco88I CYCGRG 3 cut(s) 277, 487, 629
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoO109I RGGNCCY 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 87, 211, 1035
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 8 cut(s) 13, 287, 322, 373, 497, 647, 865, 1048
FaqI GGGAC 1 cut(s) 540
FatI CATG 8 cut(s) 9, 283, 318, 369, 493, 643, 861, 1044
FauI CCCGC 4 cut(s) 166, 196, 251, 895
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 3 cut(s) 34, 76, 606
FokI GGATG 3 cut(s) 56, 863, 1018
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 3 cut(s) 34, 76, 606
GlaI GCGC 8 cut(s) 25, 61, 79, 147, 177, 207, 231, 935
GluI GCNGC 3 cut(s) 34, 76, 606
GsuI CTGGAG 3 cut(s) 29, 117, 195
HaeIII GGCC 4 cut(s) 120, 216, 248, 492
HapII CCGG 2 cut(s) 57, 630
HhaI GCGC 8 cut(s) 26, 62, 80, 148, 178, 208, 232, 936
Hin1II CATG 8 cut(s) 13, 287, 322, 373, 497, 647, 865, 1048
Hin6I GCGC 8 cut(s) 24, 60, 78, 146, 176, 206, 230, 934
HinP1I GCGC 8 cut(s) 24, 60, 78, 146, 176, 206, 230, 934
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 4 cut(s) 49, 65, 403, 467
HpaII CCGG 2 cut(s) 57, 630
HphI GGTGA 2 cut(s) 28, 383
Hpy166II GTNNAC 4 cut(s) 19, 500, 716, 896
Hpy188I TCNGA 1 cut(s) 948
Hpy188III TCNNGA 3 cut(s) 46, 353, 750
Hpy8I GTNNAC 4 cut(s) 19, 500, 716, 896
HpyAV CCTTC 3 cut(s) 238, 799, 1144
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 1 cut(s) 815
HpyCH4V TGCA 7 cut(s) 300, 318, 363, 605, 647, 699, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 84, 143
HpyF3I CTNAG 4 cut(s) 126, 573, 636, 1079
HpySE526I ACGT 1 cut(s) 815
Hsp92II CATG 8 cut(s) 13, 287, 322, 373, 497, 647, 865, 1048
HspAI GCGC 8 cut(s) 24, 60, 78, 146, 176, 206, 230, 934
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
LmnI GCTCC 2 cut(s) 166, 196
Lsp1109I GCAGC 2 cut(s) 62, 617
LweI GCATC 3 cut(s) 298, 309, 646
MaeII ACGT 1 cut(s) 815
MaeIII GTNAC 2 cut(s) 94, 1027
MalI GATC 7 cut(s) 254, 263, 735, 748, 766, 810, 845
MbiI CCGCTC 2 cut(s) 161, 191
MboI GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
MboII GAAGA 5 cut(s) 253, 368, 392, 395, 989
MfeI CAATTG 2 cut(s) 609, 885
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 291, 908
MluCI AATT 5 cut(s) 511, 609, 885, 925, 1094
MlyI GAGTC 2 cut(s) 74, 461
MseI TTAA 2 cut(s) 989, 1097
MslI CAYNNNNRTG 3 cut(s) 14, 368, 425
MspA1I CMGCKG 1 cut(s) 75
MspI CCGG 2 cut(s) 57, 630
MspR9I CCNGG 6 cut(s) 57, 89, 213, 630, 631, 1037
MunI CAATTG 2 cut(s) 609, 885
MvaI CCWGG 3 cut(s) 89, 213, 1037
MvnI CGCG 4 cut(s) 146, 176, 206, 230
MwoI GCNNNNNNNGC 3 cut(s) 30, 84, 143
NciI CCSGG 3 cut(s) 57, 630, 631
NcoI CCATGG 1 cut(s) 493
NdeII GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
NlaIII CATG 8 cut(s) 13, 287, 322, 373, 497, 647, 865, 1048
NlaIV GGNNCC 3 cut(s) 859, 907, 1089
NmuCI GTSAC 2 cut(s) 94, 1027
NspI RCATGY 3 cut(s) 13, 322, 1048
PaeI GCATGC 2 cut(s) 322, 1048
PaeR7I CTCGAG 1 cut(s) 277
PceI AGGCCT 1 cut(s) 120
PfeI GAWTC 2 cut(s) 49, 403
PkrI GCNGC 3 cut(s) 35, 77, 607
PleI GAGTC 2 cut(s) 73, 461
PpsI GAGTC 2 cut(s) 73, 461
PsiI TTATAA 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 87, 211, 1035
PspGI CCWGG 3 cut(s) 87, 211, 1035
PspN4I GGNNCC 3 cut(s) 859, 907, 1089
PspPI GGNCC 2 cut(s) 215, 490
PspXI VCTCGAGB 1 cut(s) 277
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 6 cut(s) 197, 275, 447, 678, 952, 958
RsaNI GTAC 6 cut(s) 196, 274, 446, 677, 951, 957
RseI CAYNNNNRTG 3 cut(s) 14, 368, 425
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 989, 1097
SatI GCNGC 3 cut(s) 34, 76, 606
Sau3AI GATC 7 cut(s) 252, 261, 733, 746, 764, 808, 843
Sau96I GGNCC 2 cut(s) 215, 490
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 74, 461
ScrFI CCNGG 6 cut(s) 57, 89, 213, 630, 631, 1037
SduI GDGCHC 2 cut(s) 291, 908
SetI ASST 9 cut(s) 157, 187, 291, 642, 818, 835, 859, 911, 958
SfaNI GCATC 3 cut(s) 298, 309, 646
Sfr274I CTCGAG 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 631
SmiMI CAYNNNNRTG 3 cut(s) 14, 368, 425
SmlI CTYRAG 2 cut(s) 277, 290
SmoI CTYRAG 2 cut(s) 277, 290
SphI GCATGC 2 cut(s) 322, 1048
Sse9I AATT 5 cut(s) 511, 609, 885, 925, 1094
SseBI AGGCCT 1 cut(s) 120
SstI GAGCTC 1 cut(s) 291
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 6 cut(s) 55, 87, 211, 628, 629, 1035
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 1 cut(s) 818
TaqI TCGA 2 cut(s) 278, 745
TasI AATT 5 cut(s) 511, 609, 885, 925, 1094
TatI WGTACW 4 cut(s) 273, 445, 676, 950
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 49, 403
Tru1I TTAA 2 cut(s) 989, 1097
Tru9I TTAA 2 cut(s) 989, 1097
TscAI CASTG 5 cut(s) 35, 382, 679, 799, 1069
TseFI GTSAC 2 cut(s) 94, 1027
TseI GCWGC 2 cut(s) 75, 605
Tsp45I GTSAC 2 cut(s) 94, 1027
TspDTI ATGAA 5 cut(s) 470, 540, 558, 632, 865
TspGWI ACGGA 1 cut(s) 183
TspMI CCCGGG 1 cut(s) 629
TspRI CASTG 5 cut(s) 35, 382, 679, 799, 1069
XagI CCTNNNNNAGG 1 cut(s) 747
XceI RCATGY 3 cut(s) 13, 322, 1048
XhoI CTCGAG 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 629
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.