pycom05g03650

Caspase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000588
Physical Location & Seq
Reverse (-)
4193299 .. 4193961
663 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03650.1

Sequence Viewer

Length: 474 bp
ATGCCACTTTCTGCGCATGGAAGAAAGCGCGCGGTGCTGTGTGGAGTGAGTTACAATGGGAAAAGTTACAAGCTCCAAGGAACGGTGAACGATGTTCAGTGCATGAAATACTTTCTTGTTGACATATTAGGATTTCCAGCTGCTTCCATACTCATGCTCACAGAATATGAGACGGACCCTTTCAGGATCCTAACAAAACAAAACATGCGAATGACGTTACAATGGTTGATGCAAGGCTGCCAATTAGGAAACTCGTTGGTGTTCCACTTCTCTGGCCATGGCTCAACCCAGCATGACTTTTCGAATGATGAGATTGACGGCAATGATGAAACGATATGCCCTTTTGATTTTGAGACCAAGGGGATGATCATTGATGATGAAATTAACGCCACCATTGTTAGGCCGGTGCCCCATGGTGCCAAACTTCATGCCACAGTCAATGCCTGCCATAATGGAATTGTTCTTGACCTATGA

Protein Analysis

158

Amino Acids

17.49

Weight (kDa)

5.72

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 8 - 151 4e-20 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 15
AccB1I GGYRCC 2 cut(s) 406, 416
AccII CGCG 2 cut(s) 30, 32
AciI CCGC 1 cut(s) 32
AclWI GGATC 2 cut(s) 181, 194
AcoI YGGCCR 1 cut(s) 274
AfiI CCNNNNNNNGG 2 cut(s) 82, 399
AluBI AGCT 2 cut(s) 73, 140
AluI AGCT 2 cut(s) 73, 140
Alw26I GTCTC 2 cut(s) 164, 347
AlwI GGATC 2 cut(s) 181, 194
AoxI GGCC 2 cut(s) 274, 401
ApeKI GCWGC 2 cut(s) 140, 237
AspLEI GCGC 3 cut(s) 16, 30, 32
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 411
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 1 cut(s) 186
BanI GGYRCC 2 cut(s) 406, 416
BbvI GCAGC 2 cut(s) 127, 224
BceAI ACGGC 1 cut(s) 334
BclI TGATCA 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 164, 347
BisI GCNGC 2 cut(s) 141, 238
BlsI GCNGC 2 cut(s) 142, 239
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 4 cut(s) 177, 188, 408, 418
BmsI GCATC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 4 cut(s) 76, 277, 357, 412
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 399
Bse118I RCCGGY 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 328
BseDI CCNNGG 4 cut(s) 76, 277, 357, 412
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 82, 399
BseMI GCAATG 1 cut(s) 328
BsePI GCGCGC 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 2 cut(s) 127, 224
BseYI CCCAGC 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 30, 32
BshFI GGCC 2 cut(s) 276, 403
BshNI GGYRCC 2 cut(s) 406, 416
BsiSI CCGG 1 cut(s) 404
BslI CCNNNNNNNGG 2 cut(s) 82, 399
BsmAI GTCTC 2 cut(s) 164, 347
BsmBI CGTCTC 1 cut(s) 164
BsnI GGCC 2 cut(s) 276, 403
Bso31I GGTCTC 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 2 cut(s) 186, 366
Bsp19I CCATGG 2 cut(s) 277, 412
BspACI CCGC 1 cut(s) 32
BspANI GGCC 2 cut(s) 276, 403
BspFNI CGCG 2 cut(s) 30, 32
BspLI GGNNCC 4 cut(s) 177, 188, 408, 418
BspPI GGATC 2 cut(s) 181, 194
BspT104I TTCGAA 1 cut(s) 302
BspT107I GGYRCC 2 cut(s) 406, 416
BspTNI GGTCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 4 cut(s) 76, 277, 357, 412
BssHII GCGCGC 1 cut(s) 28
BssMI GATC 2 cut(s) 186, 366
BssT1I CCWWGG 4 cut(s) 76, 277, 357, 412
Bst4CI ACNGT 2 cut(s) 85, 436
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 30, 445
BstDSI CCRYGG 2 cut(s) 277, 412
BstF5I GGATG 1 cut(s) 369
BstFNI CGCG 2 cut(s) 30, 32
BstHHI GCGC 3 cut(s) 16, 30, 32
BstKTI GATC 2 cut(s) 189, 369
BstMAI GTCTC 2 cut(s) 164, 347
BstMBI GATC 2 cut(s) 186, 366
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNSI RCATGY 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 411
BstUI CGCG 2 cut(s) 30, 32
BstV1I GCAGC 2 cut(s) 127, 224
BstX2I RGATCY 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 272
BstYI RGATCY 1 cut(s) 186
BsuRI GGCC 2 cut(s) 276, 403
BtgI CCRYGG 2 cut(s) 277, 412
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 30, 445
CfoI GCGC 3 cut(s) 16, 30, 32
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 175
CviAII CATG 8 cut(s) 17, 103, 154, 205, 278, 293, 413, 428
CviJI RGCY 6 cut(s) 73, 140, 237, 276, 282, 403
CviKI_1 RGCY 6 cut(s) 73, 140, 237, 276, 282, 403
DpnI GATC 2 cut(s) 188, 368
DpnII GATC 2 cut(s) 186, 366
EaeI YGGCCR 1 cut(s) 274
Eco130I CCWWGG 4 cut(s) 76, 277, 357, 412
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 175
EcoT14I CCWWGG 4 cut(s) 76, 277, 357, 412
ErhI CCWWGG 4 cut(s) 76, 277, 357, 412
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 8 cut(s) 20, 106, 157, 208, 281, 296, 416, 431
FatI CATG 8 cut(s) 16, 102, 153, 204, 277, 292, 412, 427
FbaI TGATCA 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 141, 238
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 2 cut(s) 141, 238
FspI TGCGCA 1 cut(s) 15
GlaI GCGC 3 cut(s) 15, 29, 31
GluI GCNGC 2 cut(s) 141, 238
GsaI CCCAGC 1 cut(s) 292
HaeIII GGCC 2 cut(s) 276, 403
HapII CCGG 1 cut(s) 404
HhaI GCGC 3 cut(s) 16, 30, 32
Hin1II CATG 8 cut(s) 20, 106, 157, 208, 281, 296, 416, 431
Hin6I GCGC 3 cut(s) 14, 28, 30
HinP1I GCGC 3 cut(s) 14, 28, 30
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaII CCGG 1 cut(s) 404
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 88, 121
Hpy188III TCNNGA 2 cut(s) 184, 464
Hpy8I GTNNAC 2 cut(s) 88, 121
HpyCH4III ACNGT 2 cut(s) 85, 436
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 102, 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 215
Hsp92II CATG 8 cut(s) 20, 106, 157, 208, 281, 296, 416, 431
HspAI GCGC 3 cut(s) 14, 28, 30
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 2 cut(s) 186, 366
LmnI GCTCC 1 cut(s) 78
LpnPI CCDG 6 cut(s) 150, 169, 258, 302, 417, 457
Lsp1109I GCAGC 2 cut(s) 127, 224
LweI GCATC 1 cut(s) 219
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 3 cut(s) 50, 65, 216
MalI GATC 2 cut(s) 188, 368
MboI GATC 2 cut(s) 186, 366
MboII GAAGA 1 cut(s) 33
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 3 cut(s) 242, 381, 456
MluNI TGGCCA 1 cut(s) 276
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 152, 209
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 404
MvnI CGCG 2 cut(s) 30, 32
MwoI GCNNNNNNNGC 1 cut(s) 34
NcoI CCATGG 2 cut(s) 277, 412
NdeII GATC 2 cut(s) 186, 366
NlaIII CATG 8 cut(s) 20, 106, 157, 208, 281, 296, 416, 431
NlaIV GGNNCC 4 cut(s) 177, 188, 408, 418
NsbI TGCGCA 1 cut(s) 15
NspI RCATGY 1 cut(s) 208
NspV TTCGAA 1 cut(s) 302
PauI GCGCGC 1 cut(s) 28
PkrI GCNGC 2 cut(s) 142, 239
PspFI CCCAGC 1 cut(s) 288
PspN4I GGNNCC 4 cut(s) 177, 188, 408, 418
PspPI GGNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 186
PteI GCGCGC 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 140
RseI CAYNNNNRTG 2 cut(s) 152, 209
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 141, 238
Sau3AI GATC 2 cut(s) 186, 366
Sau96I GGNCC 1 cut(s) 175
SduI GDGCHC 1 cut(s) 411
SetI ASST 4 cut(s) 75, 142, 218, 471
SfaNI GCATC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 2 cut(s) 152, 209
Sse9I AATT 3 cut(s) 242, 381, 456
SsiI CCGC 1 cut(s) 32
StyI CCWWGG 4 cut(s) 76, 277, 357, 412
TaaI ACNGT 2 cut(s) 85, 436
TaiI ACGT 1 cut(s) 218
TaqI TCGA 1 cut(s) 302
TasI AATT 3 cut(s) 242, 381, 456
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 104
TseI GCWGC 2 cut(s) 140, 237
TspDTI ATGAA 4 cut(s) 119, 342, 393, 416
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 175
XceI RCATGY 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.