Prupe.2G118900_v2.0.a1

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000588
Physical Location & Seq
Reverse (-)
17698478 .. 17700033
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G118900.1

Sequence Viewer

Length: 708 bp
ATGGCATTACATTGGCTTGTACAAGGCTGTCAACCTGGTGACTCCCTCCTGTTTCATTACTCTGGTCATGGTTCACGACAGAGGAATTATAATGGTGATGAAGTAGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACTCAGGGTATGATTGTTGATGATGAGATAAATGCAGTGATTGTAAGGCCCATTCCTCATGGGGTTAAGCTTCATGCAATAATAGATGCTTGTCATAGCGGCACTGTACTAGATTTGCCATTCCTTTGTCGAATGGACAGGAATGGACGATATGTATGGGAGGATCATCGCCCTCGATCAGGTGTATGGAAAGGCACAAGTGGAGGAGAAGTCATTTCGTTCAGTGGTTGTGATGATGATCAGACTTCTGCTGATACATCTGCTCTATCAAAGATCACGTCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCAATTGAACGTGGACAAGCGGGCACATATGGAAGCATACTCAATTCTATGCGTTCTACTCTTCGAAGTACAGGTAGTGGTAGTGGCCTTGGTGGTGGTGTTACATCTCTCCTTGGTGGTGGTGGTGGTGGTGTTACATCTCTCCTTGGGATGCTTCTGACAGGAGGCAGTGACACTGGCGGGTTGAGACAGGAGCCGCAATTAACTGCCTGCGAGCCATTCGATGTGTATACAAAGCCTTTCTCCCTATAA

Protein Analysis

236

Amino Acids

24.94

Weight (kDa)

5.01

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 90
AccB1I GGYRCC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 686
AciI CCGC 4 cut(s) 243, 476, 636, 653
AclWI GGATC 1 cut(s) 315
AfaI GTAC 3 cut(s) 21, 252, 526
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 1 cut(s) 464
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 637
AlwI GGATC 1 cut(s) 315
AoxI GGCC 2 cut(s) 191, 541
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AspS9I GGNCC 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 50, 107
AsuII TTCGAA 2 cut(s) 141, 520
BaeGI GKGCMC 1 cut(s) 482
BanI GGYRCC 1 cut(s) 431
BccI CCATC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 637
BfaI CTAG 1 cut(s) 254
BisI GCNGC 2 cut(s) 244, 653
BlsI GCNGC 2 cut(s) 245, 654
Bme1390I CCNGG 1 cut(s) 36
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 433, 651
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 220, 597
Bpu14I TTCGAA 2 cut(s) 141, 520
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 544, 568, 601
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 544, 568, 601
BseGI GGATG 2 cut(s) 450, 612
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 637
BseRI GAGGAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 482
BshFI GGCC 2 cut(s) 193, 543
BshNI GGYRCC 1 cut(s) 431
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 637
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 2 cut(s) 193, 543
Bsp119I TTCGAA 2 cut(s) 141, 520
Bsp1286I GDGCHC 1 cut(s) 482
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 307, 320, 382, 417
BspACI CCGC 4 cut(s) 243, 476, 636, 653
BspANI GGCC 2 cut(s) 193, 543
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 433, 651
BspPI GGATC 1 cut(s) 315
BspT104I TTCGAA 2 cut(s) 141, 520
BspT107I GGYRCC 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 19
BsrI ACTGG 1 cut(s) 637
BssECI CCNNGG 3 cut(s) 544, 568, 601
BssMI GATC 4 cut(s) 307, 320, 382, 417
BssNAI GTATAC 1 cut(s) 687
BssT1I CCWWGG 3 cut(s) 544, 568, 601
Bst1107I GTATAC 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 522
BstAUI TGTACA 1 cut(s) 19
BstBI TTCGAA 2 cut(s) 141, 520
BstC8I GCNNGC 3 cut(s) 478, 667, 671
BstDEI CTNAG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 450, 612
BstKTI GATC 4 cut(s) 310, 323, 385, 420
BstMAI GTCTC 1 cut(s) 637
BstMBI GATC 4 cut(s) 307, 320, 382, 417
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 482
BstZ17I GTATAC 1 cut(s) 687
BsuRI GGCC 2 cut(s) 193, 543
BtgZI GCGATG 1 cut(s) 296
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 2 cut(s) 450, 612
BtsI GCAGTG 2 cut(s) 186, 631
BtsIMutI CAGTG 5 cut(s) 186, 246, 373, 630, 631
Cac8I GCNNGC 3 cut(s) 478, 667, 671
Cfr13I GGNCC 1 cut(s) 192
CsiI ACCWGGT 1 cut(s) 34
Csp6I GTAC 3 cut(s) 20, 251, 525
CviAII CATG 4 cut(s) 68, 203, 218, 436
CviJI RGCY 8 cut(s) 16, 27, 193, 214, 543, 652, 673, 694
CviKI_1 RGCY 8 cut(s) 16, 27, 193, 214, 543, 652, 673, 694
CviQI GTAC 3 cut(s) 20, 251, 525
DdeI CTNAG 1 cut(s) 147
DpnI GATC 4 cut(s) 309, 322, 384, 419
DpnII GATC 4 cut(s) 307, 320, 382, 417
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Eco130I CCWWGG 3 cut(s) 544, 568, 601
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 3 cut(s) 544, 568, 601
ErhI CCWWGG 3 cut(s) 544, 568, 601
FaeI CATG 4 cut(s) 71, 206, 221, 439
FaqI GGGAC 1 cut(s) 114
FatI CATG 4 cut(s) 67, 202, 217, 435
FauI CCCGC 2 cut(s) 469, 629
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 686
Fnu4HI GCNGC 2 cut(s) 244, 653
FokI GGATG 2 cut(s) 437, 619
Fsp4HI GCNGC 2 cut(s) 244, 653
FspBI CTAG 1 cut(s) 254
GluI GCNGC 2 cut(s) 244, 653
HaeIII GGCC 2 cut(s) 193, 543
Hin1II CATG 4 cut(s) 71, 206, 221, 439
HincII GTYRAC 2 cut(s) 32, 426
HindII GTYRAC 2 cut(s) 32, 426
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 1 cut(s) 41
HphI GGTGA 2 cut(s) 50, 107
Hpy166II GTNNAC 5 cut(s) 32, 74, 426, 470, 687
Hpy188I TCNGA 2 cut(s) 387, 615
Hpy188III TCNNGA 1 cut(s) 75
Hpy8I GTNNAC 5 cut(s) 32, 74, 426, 470, 687
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 422, 466
HpyCH4V TGCA 2 cut(s) 179, 221
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 2 cut(s) 422, 466
Hsp92II CATG 4 cut(s) 71, 206, 221, 439
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 4 cut(s) 307, 320, 382, 417
LmnI GCTCC 1 cut(s) 649
LweI GCATC 2 cut(s) 220, 597
MabI ACCWGGT 1 cut(s) 34
MaeI CTAG 1 cut(s) 254
MaeII ACGT 2 cut(s) 422, 466
MaeIII GTNAC 4 cut(s) 38, 556, 589, 626
MalI GATC 4 cut(s) 309, 322, 384, 419
MboI GATC 4 cut(s) 307, 320, 382, 417
MboII GAAGA 1 cut(s) 509
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 4 cut(s) 85, 459, 499, 656
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 7 cut(s) 56, 75, 210, 298, 327, 341, 614
MseI TTAA 2 cut(s) 210, 659
MspR9I CCNGG 1 cut(s) 36
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 484
NdeII GATC 4 cut(s) 307, 320, 382, 417
NlaIII CATG 4 cut(s) 71, 206, 221, 439
NlaIV GGNNCC 2 cut(s) 433, 651
NmuCI GTSAC 2 cut(s) 38, 626
NspV TTCGAA 2 cut(s) 141, 520
PkrI GCNGC 2 cut(s) 245, 654
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PsiI TTATAA 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 433, 651
PspPI GGNCC 1 cut(s) 192
RsaI GTAC 3 cut(s) 21, 252, 526
RsaNI GTAC 3 cut(s) 20, 251, 525
SaqAI TTAA 2 cut(s) 210, 659
SatI GCNGC 2 cut(s) 244, 653
Sau3AI GATC 4 cut(s) 307, 320, 382, 417
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 482
SetI ASST 7 cut(s) 37, 216, 328, 425, 433, 469, 532
SexAI ACCWGGT 1 cut(s) 34
SfaNI GCATC 2 cut(s) 220, 597
SfuI TTCGAA 2 cut(s) 141, 520
Sse9I AATT 4 cut(s) 85, 459, 499, 656
SsiI CCGC 4 cut(s) 243, 476, 636, 653
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 3 cut(s) 544, 568, 601
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 2 cut(s) 425, 469
TaqI TCGA 5 cut(s) 141, 274, 319, 520, 678
TasI AATT 4 cut(s) 85, 459, 499, 656
TatI WGTACW 3 cut(s) 19, 250, 524
TauI GCSGC 2 cut(s) 246, 655
Tru1I TTAA 2 cut(s) 210, 659
Tru9I TTAA 2 cut(s) 210, 659
TscAI CASTG 5 cut(s) 186, 253, 373, 631, 637
TseFI GTSAC 2 cut(s) 38, 626
Tsp45I GTSAC 2 cut(s) 38, 626
TspDTI ATGAA 5 cut(s) 44, 114, 132, 206, 439
TspRI CASTG 5 cut(s) 186, 253, 373, 631, 637
XagI CCTNNNNNAGG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 686
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.