pycom02g18780

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000588
Physical Location & Seq
Forward (+)
16879033 .. 16881799
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18780.3

Sequence Viewer

Length: 804 bp
ATGTATAACATGCTGGTGAACTGCGCCCACTGCCGCACCCCTCTCCAGATTCCCCCCGGCGCAGAGTCCATCCGCTGCGCCATCTGCCAGGGTGTCACTCTAATCGCCGACCCACGCGGCCTCCCTCAGCCTCCAGCCTCCCACGCGCCTCCACCTCCCGCTCCATATTCTCACGCGCCTCCACCTCCCTCTCCATACTCTCACGCGCCTCCAGGCCCTCCCCCCAACGCTCACGGGCGGAAGAAGGCCGTGATCTGCGGGATCTCGTATAAGTACTCGAGGCATGAGCTCAAGGGTTGCATCAATGATGCCAAGTGCATGCGGTATCTCCTCATCAACAAGTTCCAGTTTCCAGAAGATTGCATTGTCATGCTCACTGAAGAAGAAACTCACCCCTCCAAGATTCCAAACAAATATAACATTAGAATGGCATTATATTGGCTTGTACAAGGCTGTCAAGCAGGCGACTCCCTATTTTTTCATTACTCTGGCCATGGTTCACGGCAGAGGAATTATAATGGCGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACTCAGGGTATGATTGTTGATGATGAGATAAATGCAGCAATTGTGAGACCCATTCCACCCGGGGCTAAGCTTCATGCAATAATAGATGCTTGTCATAGTGGCACTGTACTGGATTTGCCATTCCTTTGCAGAATGGACAGGAGTGGACGATTTGAATGGGAGGATCGATCGCCCTCGATCAGGCATGTGGAAAGGATCAGGCGGTGGAGAAGTCATTTCCTTCAGTGGTTGTGA

Protein Analysis

268

Amino Acids

30.1

Weight (kDa)

7.59

Isoelectric Point (pI)

57.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AccBSI CCGCTC 1 cut(s) 161
AccII CGCG 4 cut(s) 117, 146, 176, 206
AciI CCGC 8 cut(s) 34, 73, 117, 159, 238, 258, 322, 772
AclWI GGATC 3 cut(s) 269, 741, 773
AcoI YGGCCR 1 cut(s) 490
AcuI CTGAAG 2 cut(s) 399, 776
AfaI GTAC 3 cut(s) 275, 447, 678
AfiI CCNNNNNNNGG 1 cut(s) 750
AgsI TTSAA 1 cut(s) 725
AjnI CCWGG 2 cut(s) 87, 211
AluBI AGCT 2 cut(s) 289, 640
AluI AGCT 2 cut(s) 289, 640
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 610
AlwI GGATC 3 cut(s) 269, 741, 773
Ama87I CYCGRG 2 cut(s) 277, 629
AoxI GGCC 4 cut(s) 118, 214, 246, 490
ApeKI GCWGC 2 cut(s) 75, 605
AspLEI GCGC 6 cut(s) 26, 62, 80, 148, 178, 208
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 3 cut(s) 57, 630, 631
AsuHPI GGTGA 2 cut(s) 28, 383
AsuII TTCGAA 1 cut(s) 567
AvaI CYCGRG 2 cut(s) 277, 629
BalI TGGCCA 1 cut(s) 492
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 62, 617
BccI CCATC 3 cut(s) 77, 89, 527
BceAI ACGGC 2 cut(s) 233, 518
BciT130I CCWGG 2 cut(s) 89, 213
BcnI CCSGG 3 cut(s) 57, 630, 631
BcoDI GTCTC 1 cut(s) 610
BisI GCNGC 4 cut(s) 34, 76, 118, 606
BlpI GCTNAGC 1 cut(s) 636
BlsI GCNGC 4 cut(s) 35, 77, 119, 607
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 5 cut(s) 57, 89, 213, 630, 631
BmeT110I CYCGRG 2 cut(s) 277, 629
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 5 cut(s) 57, 89, 213, 630, 631
BmsI GCATC 3 cut(s) 298, 309, 646
BpmI CTGGAG 3 cut(s) 29, 117, 195
Bpu10I CCTNAGC 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 3 cut(s) 57, 630, 631
Bsa29I ATCGAT 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 5 cut(s) 55, 88, 493, 629, 630
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 2 cut(s) 346, 684
BseBI CCWGG 2 cut(s) 89, 213
BseCI ATCGAT 1 cut(s) 736
BseDI CCNNGG 5 cut(s) 55, 88, 493, 629, 630
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMII CTCAG 2 cut(s) 140, 587
BseNI ACTGG 2 cut(s) 346, 684
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 62, 617
Bsh1236I CGCG 4 cut(s) 117, 146, 176, 206
Bsh1285I CGRYCG 1 cut(s) 740
BshFI GGCC 4 cut(s) 120, 216, 248, 492
BshVI ATCGAT 1 cut(s) 736
BsiEI CGRYCG 1 cut(s) 740
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 2 cut(s) 277, 629
BsiSI CCGG 2 cut(s) 57, 630
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 1 cut(s) 610
BsmFI GGGAC 1 cut(s) 540
BsnI GGCC 4 cut(s) 120, 216, 248, 492
Bso31I GGTCTC 1 cut(s) 610
BsoBI CYCGRG 2 cut(s) 277, 629
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 291
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 6 cut(s) 252, 261, 733, 737, 747, 765
Bsp1720I GCTNAGC 1 cut(s) 636
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 8 cut(s) 34, 73, 117, 159, 238, 258, 322, 772
BspANI GGCC 4 cut(s) 120, 216, 248, 492
BspCNI CTCAG 2 cut(s) 139, 586
BspDI ATCGAT 1 cut(s) 736
BspFNI CGCG 4 cut(s) 117, 146, 176, 206
BspPI GGATC 3 cut(s) 269, 741, 773
BspT104I TTCGAA 1 cut(s) 567
BspTNI GGTCTC 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 2 cut(s) 346, 684
BssECI CCNNGG 5 cut(s) 55, 88, 493, 629, 630
BssMI GATC 6 cut(s) 252, 261, 733, 737, 747, 765
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 2 cut(s) 89, 213
Bst4CI ACNGT 1 cut(s) 676
BstAUI TGTACA 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 320, 463
BstDEI CTNAG 3 cut(s) 126, 573, 636
BstDSI CCRYGG 1 cut(s) 493
BstENI CCTNNNNNAGG 1 cut(s) 748
BstF5I GGATG 1 cut(s) 69
BstFNI CGCG 4 cut(s) 117, 146, 176, 206
BstHHI GCGC 6 cut(s) 26, 62, 80, 148, 178, 208
BstKTI GATC 6 cut(s) 255, 264, 736, 740, 750, 768
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 6 cut(s) 252, 261, 733, 737, 747, 765
BstMCI CGRYCG 1 cut(s) 740
BstMWI GCNNNNNNNGC 3 cut(s) 30, 84, 143
BstNI CCWGG 2 cut(s) 89, 213
BstNSI RCATGY 3 cut(s) 13, 322, 758
BstSCI CCNGG 5 cut(s) 55, 87, 211, 628, 629
BstUI CGCG 4 cut(s) 117, 146, 176, 206
BstV1I GCAGC 2 cut(s) 62, 617
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
Bsu15I ATCGAT 1 cut(s) 736
BsuRI GGCC 4 cut(s) 120, 216, 248, 492
BsuTUI ATCGAT 1 cut(s) 736
BtgI CCRYGG 1 cut(s) 493
BtgZI GCGATG 1 cut(s) 537
BtsCI GGATG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 4 cut(s) 28, 375, 672, 800
Cac8I GCNNGC 2 cut(s) 320, 463
CfoI GCGC 6 cut(s) 26, 62, 80, 148, 178, 208
Cfr13I GGNCC 1 cut(s) 215
Cfr9I CCCGGG 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 736
Csp6I GTAC 3 cut(s) 274, 446, 677
CviAII CATG 7 cut(s) 10, 284, 319, 370, 494, 644, 755
CviQI GTAC 3 cut(s) 274, 446, 677
DdeI CTNAG 3 cut(s) 126, 573, 636
DpnI GATC 6 cut(s) 254, 263, 735, 739, 749, 767
DpnII GATC 6 cut(s) 252, 261, 733, 737, 747, 765
EaeI YGGCCR 1 cut(s) 490
EciI GGCGGA 1 cut(s) 253
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 399, 776
Eco88I CYCGRG 2 cut(s) 277, 629
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 748
EcoO109I RGGNCCY 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 87, 211
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 7 cut(s) 13, 287, 322, 373, 497, 647, 758
FaqI GGGAC 1 cut(s) 540
FatI CATG 7 cut(s) 9, 283, 318, 369, 493, 643, 754
FauI CCCGC 2 cut(s) 166, 251
Fnu4HI GCNGC 4 cut(s) 34, 76, 118, 606
FokI GGATG 1 cut(s) 56
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 4 cut(s) 34, 76, 118, 606
GlaI GCGC 6 cut(s) 25, 61, 79, 147, 177, 207
GluI GCNGC 4 cut(s) 34, 76, 118, 606
GsuI CTGGAG 3 cut(s) 29, 117, 195
HaeIII GGCC 4 cut(s) 120, 216, 248, 492
HapII CCGG 2 cut(s) 57, 630
HhaI GCGC 6 cut(s) 26, 62, 80, 148, 178, 208
Hin1II CATG 7 cut(s) 13, 287, 322, 373, 497, 647, 758
Hin6I GCGC 6 cut(s) 24, 60, 78, 146, 176, 206
HinP1I GCGC 6 cut(s) 24, 60, 78, 146, 176, 206
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 4 cut(s) 49, 65, 403, 467
HpaII CCGG 2 cut(s) 57, 630
HphI GGTGA 2 cut(s) 28, 383
Hpy166II GTNNAC 3 cut(s) 19, 500, 716
Hpy188III TCNNGA 2 cut(s) 46, 353
Hpy8I GTNNAC 3 cut(s) 19, 500, 716
HpyAV CCTTC 2 cut(s) 238, 800
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4V TGCA 6 cut(s) 300, 318, 363, 605, 647, 699
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 84, 143
HpyF3I CTNAG 3 cut(s) 126, 573, 636
Hsp92II CATG 7 cut(s) 13, 287, 322, 373, 497, 647, 758
HspAI GCGC 6 cut(s) 24, 60, 78, 146, 176, 206
Kzo9I GATC 6 cut(s) 252, 261, 733, 737, 747, 765
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 2 cut(s) 62, 617
LweI GCATC 3 cut(s) 298, 309, 646
MaeIII GTNAC 1 cut(s) 94
MalI GATC 6 cut(s) 254, 263, 735, 739, 749, 767
MbiI CCGCTC 1 cut(s) 161
MboI GATC 6 cut(s) 252, 261, 733, 737, 747, 765
MboII GAAGA 4 cut(s) 253, 368, 392, 395
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 2 cut(s) 511, 609
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 2 cut(s) 74, 461
Mox20I TGGCCA 1 cut(s) 492
MscI TGGCCA 1 cut(s) 492
MslI CAYNNNNRTG 3 cut(s) 14, 368, 425
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 75
MspI CCGG 2 cut(s) 57, 630
MspR9I CCNGG 5 cut(s) 57, 89, 213, 630, 631
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 2 cut(s) 89, 213
MvnI CGCG 4 cut(s) 117, 146, 176, 206
MwoI GCNNNNNNNGC 3 cut(s) 30, 84, 143
NciI CCSGG 3 cut(s) 57, 630, 631
NcoI CCATGG 1 cut(s) 493
NdeII GATC 6 cut(s) 252, 261, 733, 737, 747, 765
NlaIII CATG 7 cut(s) 13, 287, 322, 373, 497, 647, 758
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 3 cut(s) 13, 322, 758
NspV TTCGAA 1 cut(s) 567
PaeI GCATGC 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 277
PfeI GAWTC 2 cut(s) 49, 403
PkrI GCNGC 4 cut(s) 35, 77, 119, 607
Ple19I CGATCG 1 cut(s) 740
PleI GAGTC 2 cut(s) 73, 461
PpsI GAGTC 2 cut(s) 73, 461
PsiI TTATAA 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 87, 211
PspGI CCWGG 2 cut(s) 87, 211
PspPI GGNCC 1 cut(s) 215
PspXI VCTCGAGB 1 cut(s) 277
PsuI RGATCY 1 cut(s) 261
PvuI CGATCG 1 cut(s) 740
RsaI GTAC 3 cut(s) 275, 447, 678
RsaNI GTAC 3 cut(s) 274, 446, 677
RseI CAYNNNNRTG 3 cut(s) 14, 368, 425
SacI GAGCTC 1 cut(s) 291
SatI GCNGC 4 cut(s) 34, 76, 118, 606
Sau3AI GATC 6 cut(s) 252, 261, 733, 737, 747, 765
Sau96I GGNCC 1 cut(s) 215
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 74, 461
ScrFI CCNGG 5 cut(s) 57, 89, 213, 630, 631
SduI GDGCHC 1 cut(s) 291
SetI ASST 4 cut(s) 157, 187, 291, 642
SfaNI GCATC 3 cut(s) 298, 309, 646
Sfr274I CTCGAG 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 567
SlaI CTCGAG 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 631
SmiMI CAYNNNNRTG 3 cut(s) 14, 368, 425
SmlI CTYRAG 2 cut(s) 277, 290
SmoI CTYRAG 2 cut(s) 277, 290
SphI GCATGC 1 cut(s) 322
Sse9I AATT 2 cut(s) 511, 609
SsiI CCGC 8 cut(s) 34, 73, 117, 159, 238, 258, 322, 772
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 5 cut(s) 55, 87, 211, 628, 629
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 676
TaqI TCGA 4 cut(s) 278, 567, 736, 746
TasI AATT 2 cut(s) 511, 609
TatI WGTACW 3 cut(s) 273, 445, 676
TauI GCSGC 2 cut(s) 36, 120
TfiI GAWTC 2 cut(s) 49, 403
TscAI CASTG 4 cut(s) 35, 382, 679, 800
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 75, 605
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 4 cut(s) 470, 540, 558, 632
TspMI CCCGGG 1 cut(s) 629
TspRI CASTG 4 cut(s) 35, 382, 679, 800
XagI CCTNNNNNAGG 1 cut(s) 748
XceI RCATGY 3 cut(s) 13, 322, 758
XhoI CTCGAG 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 629
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.