MD05G1044000.v1.1

Caspase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000588
Physical Location & Seq
Reverse (-)
7533266 .. 7535620
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1044000.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCAAGTCGGATTGAGAGGTGCAATGGGTGTGGACGGAAACTTGTAGTGCCATTACAAGCACAAATCATTCGTTGTGGTGCGTGTCAAGGCGTCCCGCGCATCAATCGAACATCCATCAATCCTCTGATTCGAGCGAGCCATGATTCAATAAACCACGCTGTCAACCGTCTTAAAAGCCAAATGACTAGGGTGATGACCATGCCCTCCCCGAATGCGGGCCGCCCTGCTAATCCTAGATACTACCATCATCAGCTGTCCCGGCCATCAGTACCAAAACCCTTGATGCCACCTTCGACGCATGGAAGAAAGCTCGCGGTGCTGTGTGGAGTGAGTTACAATGGGAAAAGTTACAAGCTCCAAGGAACGGTGAACGATGTTCAGTGCATGAAATACTTTCTTGTTGACAGATTGGGATTTCCAACTGCTTCCATACTCATGCTCACAGAATATGAGACAGACCCTTTCAGGATCCCAACAAAACAAAACATGCGTATGGCGTTACAGTGGTTGATGCAGGGCTGCCAATCAGGAGACTCGTTGTTGTTCCACTTCTCTGGCCATGGCTCAACCCAGCATGACTTTTCGAATGATGAGATTGACGGCAATGATGAAACGGTGTGCCCTGTTGATTTTGAGACCGAGGGGATGATCATTGATGATGAAATTAACGCCACCATTGTTAGGCCGGTGCCCCATGGTGCCAAACTTCATGCCATTGTCGATGCCTGCCATAGTGGAACTGTTCTTGACCTACGATTTGTTTGCAGGATCAACAGAGAAGGATATTCGTGGGAAGATCAACAATACTCGCCTATTTATAAGGGCACAAGTGGTGGACCAGCTGTCTTCATAAGCGCTTGTGACGACCATCAAAAATCTGCAGACACCACTGTGAGTATGTATAATCGATATGTTTTGTCTGGAGACACTTCAACGGGTGCCTTGACTTATAGCTTTATCCAAGCAGCGGAAAATGATCGCGGATCGACATATGGCTCTTTACTGAATGCTATGCGCCACAAAATTCGTGAAGCTAAAACTGGCATTGATATGGAGACCCAGTTGTCATCATCTGAGATATTTGACATTTGCTCAAAACAATTGGTATTGCAACTTAGAAATTAA

Protein Analysis

376

Amino Acids

41.71

Weight (kDa)

8.22

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 103 - 349 8.9e-33 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AasI GACNNNNNNGTC 1 cut(s) 1066
AccB1I GGYRCC 3 cut(s) 691, 701, 941
AccII CGCG 3 cut(s) 100, 317, 984
AciI CCGC 6 cut(s) 98, 218, 223, 317, 971, 984
AclWI GGATC 4 cut(s) 466, 479, 779, 994
AcoI YGGCCR 2 cut(s) 263, 559
AcsI RAATTY 1 cut(s) 1026
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 273
AfeI AGCGCT 1 cut(s) 859
AfiI CCNNNNNNNGG 5 cut(s) 217, 218, 367, 684, 970
AgsI TTSAA 2 cut(s) 150, 936
AhdI GACNNNNNGTC 1 cut(s) 845
AleI CACNNNNGTG 1 cut(s) 893
AluBI AGCT 6 cut(s) 256, 313, 358, 845, 957, 1037
AluI AGCT 6 cut(s) 256, 313, 358, 845, 957, 1037
Alw26I GTCTC 5 cut(s) 449, 528, 632, 921, 1052
AlwI GGATC 4 cut(s) 466, 479, 779, 994
Aor51HI AGCGCT 1 cut(s) 859
AoxI GGCC 4 cut(s) 220, 263, 559, 686
ApeKI GCWGC 2 cut(s) 522, 968
ApoI RAATTY 1 cut(s) 1026
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AspLEI GCGC 3 cut(s) 102, 860, 1020
AspS9I GGNCC 2 cut(s) 220, 839
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 205, 382
AsuII TTCGAA 1 cut(s) 587
AvaII GGWCC 1 cut(s) 839
BaeGI GKGCMC 3 cut(s) 626, 696, 830
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 1 cut(s) 471
BanI GGYRCC 3 cut(s) 691, 701, 941
BbsI GAAGAC 1 cut(s) 841
BbvI GCAGC 2 cut(s) 509, 980
BccI CCATC 4 cut(s) 125, 255, 274, 879
BceAI ACGGC 1 cut(s) 619
BcgI CGANNNNNNTGC 2 cut(s) 747, 781
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 5 cut(s) 449, 528, 632, 921, 1052
BfaI CTAG 2 cut(s) 189, 237
BfmI CTRYAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 861
BisI GCNGC 3 cut(s) 223, 523, 969
BlsI GCNGC 3 cut(s) 224, 524, 970
Bme1390I CCNGG 1 cut(s) 262
Bme18I GGWCC 1 cut(s) 839
BmeRI GACNNNNNGTC 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 220, 839
BmiI GGNNCC 4 cut(s) 473, 693, 703, 943
BmrFI CCNGG 1 cut(s) 262
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 4 cut(s) 111, 276, 504, 715
BmuI ACTGGG 1 cut(s) 1057
BpiI GAAGAC 1 cut(s) 841
BpmI CTGGAG 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 587
BpuMI CCSGG 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 910
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 2 cut(s) 632, 1052
BsaJI CCNNGG 4 cut(s) 361, 562, 642, 697
BsaXI ACNNNNNCTCC 2 cut(s) 1049, 1079
Bsc4I CCNNNNNNNGG 5 cut(s) 217, 218, 367, 684, 970
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 2 cut(s) 1048, 1063
Bse3DI GCAATG 2 cut(s) 31, 613
BseCI ATCGAT 1 cut(s) 910
BseDI CCNNGG 4 cut(s) 361, 562, 642, 697
BseGI GGATG 2 cut(s) 113, 654
BseLI CCNNNNNNNGG 5 cut(s) 217, 218, 367, 684, 970
BseMI GCAATG 2 cut(s) 31, 613
BseMII CTCAG 1 cut(s) 1068
BseNI ACTGG 2 cut(s) 1048, 1063
BseSI GKGCMC 3 cut(s) 626, 696, 830
BseXI GCAGC 2 cut(s) 509, 980
BseYI CCCAGC 1 cut(s) 573
Bsh1236I CGCG 3 cut(s) 100, 317, 984
BshFI GGCC 4 cut(s) 222, 265, 561, 688
BshNI GGYRCC 3 cut(s) 691, 701, 941
BshVI ATCGAT 1 cut(s) 910
BsiSI CCGG 2 cut(s) 262, 689
BslFI GGGAC 2 cut(s) 80, 244
BslI CCNNNNNNNGG 5 cut(s) 217, 218, 367, 684, 970
BsmAI GTCTC 5 cut(s) 449, 528, 632, 921, 1052
BsmFI GGGAC 2 cut(s) 80, 244
BsmI GAATGC 2 cut(s) 220, 1015
BsnI GGCC 4 cut(s) 222, 265, 561, 688
Bso31I GGTCTC 2 cut(s) 632, 1052
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 3 cut(s) 626, 696, 830
Bsp143I GATC 6 cut(s) 471, 651, 771, 799, 979, 986
Bsp19I CCATGG 2 cut(s) 562, 697
BspACI CCGC 6 cut(s) 98, 218, 223, 317, 971, 984
BspANI GGCC 4 cut(s) 222, 265, 561, 688
BspCNI CTCAG 1 cut(s) 1069
BspDI ATCGAT 1 cut(s) 910
BspFNI CGCG 3 cut(s) 100, 317, 984
BspLI GGNNCC 4 cut(s) 473, 693, 703, 943
BspMAI CTGCAG 1 cut(s) 886
BspPI GGATC 4 cut(s) 466, 479, 779, 994
BspT104I TTCGAA 1 cut(s) 587
BspT107I GGYRCC 3 cut(s) 691, 701, 941
BspTNI GGTCTC 2 cut(s) 632, 1052
BsrDI GCAATG 2 cut(s) 31, 613
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 2 cut(s) 1048, 1063
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 361, 562, 642, 697
BssMI GATC 6 cut(s) 471, 651, 771, 799, 979, 986
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 3 cut(s) 361, 562, 697
Bst4CI ACNGT 6 cut(s) 170, 370, 507, 619, 745, 895
BstACI GRCGYC 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 4 cut(s) 139, 220, 315, 730
BstDEI CTNAG 2 cut(s) 1077, 1118
BstDSI CCRYGG 2 cut(s) 562, 697
BstF5I GGATG 2 cut(s) 113, 654
BstFNI CGCG 3 cut(s) 100, 317, 984
BstH2I RGCGCY 1 cut(s) 861
BstHHI GCGC 3 cut(s) 102, 860, 1020
BstKTI GATC 6 cut(s) 474, 654, 774, 802, 982, 989
BstMAI GTCTC 5 cut(s) 449, 528, 632, 921, 1052
BstMBI GATC 6 cut(s) 471, 651, 771, 799, 979, 986
BstMWI GCNNNNNNNGC 3 cut(s) 99, 262, 319
BstNSI RCATGY 1 cut(s) 493
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 3 cut(s) 626, 696, 830
BstUI CGCG 3 cut(s) 100, 317, 984
BstV1I GCAGC 2 cut(s) 509, 980
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 1 cut(s) 471
Bsu15I ATCGAT 1 cut(s) 910
BsuRI GGCC 4 cut(s) 222, 265, 561, 688
BsuTUI ATCGAT 1 cut(s) 910
BtgI CCRYGG 2 cut(s) 562, 697
BtsCI GGATG 2 cut(s) 113, 654
BtsIMutI CAGTG 3 cut(s) 389, 512, 891
Cac8I GCNNGC 4 cut(s) 139, 220, 315, 730
CfoI GCGC 3 cut(s) 102, 860, 1020
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 220, 839
ClaI ATCGAT 1 cut(s) 910
CseI GACGC 2 cut(s) 82, 307
Csp6I GTAC 1 cut(s) 272
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 2 cut(s) 1077, 1118
DpnI GATC 6 cut(s) 473, 653, 773, 801, 981, 988
DpnII GATC 6 cut(s) 471, 651, 771, 799, 979, 986
DrdI GACNNNNNNGTC 1 cut(s) 1066
DriI GACNNNNNGTC 1 cut(s) 845
DseDI GACNNNNNNGTC 1 cut(s) 1066
EaeI YGGCCR 2 cut(s) 263, 559
Eam1105I GACNNNNNGTC 1 cut(s) 845
Eco130I CCWWGG 3 cut(s) 361, 562, 697
Eco31I GGTCTC 2 cut(s) 632, 1052
Eco47I GGWCC 1 cut(s) 839
Eco47III AGCGCT 1 cut(s) 859
EcoT14I CCWWGG 3 cut(s) 361, 562, 697
ErhI CCWWGG 3 cut(s) 361, 562, 697
FaqI GGGAC 2 cut(s) 80, 244
FauI CCCGC 2 cut(s) 105, 211
FauNDI CATATG 1 cut(s) 994
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 3 cut(s) 223, 523, 969
FokI GGATG 2 cut(s) 100, 661
Fsp4HI GCNGC 3 cut(s) 223, 523, 969
FspBI CTAG 2 cut(s) 189, 237
GlaI GCGC 3 cut(s) 101, 859, 1019
GluI GCNGC 3 cut(s) 223, 523, 969
GsaI CCCAGC 1 cut(s) 577
GsuI CTGGAG 1 cut(s) 945
HaeII RGCGCY 1 cut(s) 861
HaeIII GGCC 4 cut(s) 222, 265, 561, 688
HapII CCGG 2 cut(s) 262, 689
HgaI GACGC 2 cut(s) 82, 307
HhaI GCGC 3 cut(s) 102, 860, 1020
Hin1I GRCGYC 1 cut(s) 93
Hin6I GCGC 3 cut(s) 100, 858, 1018
HinP1I GCGC 3 cut(s) 100, 858, 1018
HincII GTYRAC 2 cut(s) 166, 406
HindII GTYRAC 2 cut(s) 166, 406
HinfI GANTC 3 cut(s) 130, 146, 536
HpaII CCGG 2 cut(s) 262, 689
HphI GGTGA 2 cut(s) 205, 382
Hpy166II GTNNAC 5 cut(s) 35, 166, 373, 406, 839
Hpy188I TCNGA 3 cut(s) 12, 129, 1078
Hpy188III TCNNGA 5 cut(s) 469, 531, 749, 924, 1031
Hpy8I GTNNAC 5 cut(s) 35, 166, 373, 406, 839
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 2 cut(s) 303, 776
HpyCH4III ACNGT 6 cut(s) 170, 370, 507, 619, 745, 895
HpyCH4V TGCA 6 cut(s) 24, 387, 517, 768, 884, 1114
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 262, 319
HpyF3I CTNAG 2 cut(s) 1077, 1118
Hsp92I GRCGYC 1 cut(s) 93
HspAI GCGC 3 cut(s) 100, 858, 1018
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 6 cut(s) 471, 651, 771, 799, 979, 986
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 2 cut(s) 509, 980
LweI GCATC 4 cut(s) 111, 276, 504, 715
MaeI CTAG 2 cut(s) 189, 237
MaeIII GTNAC 4 cut(s) 335, 350, 501, 863
MalI GATC 6 cut(s) 473, 653, 773, 801, 981, 988
MboI GATC 6 cut(s) 471, 651, 771, 799, 979, 986
MboII GAAGA 3 cut(s) 318, 809, 841
MfeI CAATTG 1 cut(s) 1103
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 3 cut(s) 626, 696, 830
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 4 cut(s) 666, 1026, 1103, 1123
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 4 cut(s) 12, 135, 217, 637
Mox20I TGGCCA 1 cut(s) 561
MscI TGGCCA 1 cut(s) 561
MseI TTAA 3 cut(s) 174, 669, 1126
MslI CAYNNNNRTG 4 cut(s) 437, 494, 893, 1052
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 3 cut(s) 256, 845, 971
MspI CCGG 2 cut(s) 262, 689
MspR9I CCNGG 1 cut(s) 262
MunI CAATTG 1 cut(s) 1103
Mva1269I GAATGC 2 cut(s) 220, 1015
MvnI CGCG 3 cut(s) 100, 317, 984
MwoI GCNNNNNNNGC 3 cut(s) 99, 262, 319
NciI CCSGG 1 cut(s) 262
NcoI CCATGG 2 cut(s) 562, 697
NdeI CATATG 1 cut(s) 994
NdeII GATC 6 cut(s) 471, 651, 771, 799, 979, 986
NlaIV GGNNCC 4 cut(s) 473, 693, 703, 943
NmuCI GTSAC 1 cut(s) 863
NspI RCATGY 1 cut(s) 493
NspV TTCGAA 1 cut(s) 587
OliI CACNNNNGTG 1 cut(s) 893
PctI GAATGC 2 cut(s) 220, 1015
PfeI GAWTC 2 cut(s) 130, 146
PkrI GCNGC 3 cut(s) 224, 524, 970
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
PsiI TTATAA 1 cut(s) 822
PspFI CCCAGC 1 cut(s) 573
PspN4I GGNNCC 4 cut(s) 473, 693, 703, 943
PspPI GGNCC 2 cut(s) 220, 839
PstI CTGCAG 1 cut(s) 886
PsuI RGATCY 1 cut(s) 471
PvuII CAGCTG 2 cut(s) 256, 845
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
RseI CAYNNNNRTG 4 cut(s) 437, 494, 893, 1052
SaqAI TTAA 3 cut(s) 174, 669, 1126
SatI GCNGC 3 cut(s) 223, 523, 969
Sau3AI GATC 6 cut(s) 471, 651, 771, 799, 979, 986
Sau96I GGNCC 2 cut(s) 220, 839
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 3 cut(s) 626, 696, 830
SetI ASST 9 cut(s) 23, 258, 295, 315, 360, 756, 847, 959, 1039
SfaNI GCATC 4 cut(s) 111, 276, 504, 715
SfcI CTRYAG 1 cut(s) 882
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 1 cut(s) 839
SmiMI CAYNNNNRTG 4 cut(s) 437, 494, 893, 1052
Sse9I AATT 4 cut(s) 666, 1026, 1103, 1123
SsiI CCGC 6 cut(s) 98, 218, 223, 317, 971, 984
SspMI CTAG 2 cut(s) 189, 237
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 3 cut(s) 361, 562, 697
TaaI ACNGT 6 cut(s) 170, 370, 507, 619, 745, 895
TaqI TCGA 7 cut(s) 109, 133, 296, 587, 723, 910, 989
TaqII GACCGA 1 cut(s) 656
TasI AATT 4 cut(s) 666, 1026, 1103, 1123
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 2 cut(s) 130, 146
Tru1I TTAA 3 cut(s) 174, 669, 1126
Tru9I TTAA 3 cut(s) 174, 669, 1126
TscAI CASTG 3 cut(s) 389, 512, 898
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 2 cut(s) 522, 968
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 5 cut(s) 404, 627, 678, 701, 841
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 3 cut(s) 389, 512, 898
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 1 cut(s) 1026
XceI RCATGY 1 cut(s) 493
XspI CTAG 2 cut(s) 189, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.