RchiOBHm_Chr1g0344881

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000588
Physical Location & Seq
Reverse (-)
37230204 .. 37232684
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57128

Sequence Viewer

Length: 1164 bp
ATGATGATGAGCAATTATGGAGGCCAACGACTACTAGTGGTCGACTGTTGCTATTGCCAGACCCAAATCCAGCTGCCTTCTGTAAGTCCGATATACATCGTCGGCACATCCGTCCGCTGCGGCCGCTGCTATGCCAGTACCCGCGTCGCCTCTCCGGGCTTCCCCCGCTCCCCTTATAGTGCTGTACCCTACTATGTCCCTATAAACCCACCACCACACGCCGGCTTCTCCCACAGCTCACCAGGACCTCCGCCGAACGTGCACAGGCCGAAGAAGGCGGTGATCTGCGGGATATCGTATAGGTACTCGAGGCATGAGCTCAAAGGTTGCATCAATGACGCCAAAAACATGCGGTATCTCCTCATGAGCAAGTTCAAGTTTCCAGAAGATTCCATTTGCATGCTCACTGAAGAAGAAACTCACCCATATAAGATTCCATATAAAAACAACATTAGAATGGCATTAGATTGGCTTGTACAAGGATGCCAACCAGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGGCAGTTGGATTATGATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACCCAGGGTATGATTGATGATGATGAGATAAATGCAGCAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTCTTGTCATAGTGGCACCATACTGGATTTGCCATTCCTTTGCAGAATGAACATGGGTGGAATATATGTATGGGAGGATCATCGCCCTCCATCAGGAGTATGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGACTATCAAACGTCTGCGGAAACATCAGCTCTATCCAAGATCACTTCAACAGGTGCCATGATTTTCTGCTTCATCCAAGCAATCGAGAATGGACAGGCAGCCACATATGGGAGCTTACTCAATTCTATGCGTAGTACCATTCGAAGTATGGGTTCTGCTGGTGGTGGTAGGCTAAGACAGGTATTGGCTTGCACAACATTCTTTGCATTGCTGTTGAGATTTAATGATAGTCGTCGTTATATCACCATGGGCTTTCTATATAAAAACAAGCTTCATCTGCAAATGAGTCTCATTTTTTTTTTTGAGGGCAACGGAAGACTAATCCATTAA

Protein Analysis

387

Amino Acids

43.06

Weight (kDa)

7.53

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 91 - 326 2.7e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02170
fragaria_vesca FvH4_7g08970 FvH4_7g08970 FvH4_7g08970 FvH4_7g26490
malus_domestica MD01G1173500.v1.1 MD02G1221900.v1.1 MD05G1044000.v1.1 MD07G1094600.v1.1 MD07G1240400.v1.1 MD07G1240500.v1.1 MD10G1051100.v1.1 MD10G1051200.v1.1
prunus_persica Prupe.2G118900_v2.0.a1 Prupe.2G269200_v2.0.a1 Prupe.8G059400_v2.0.a1
pyrus_communis pycom01g18530 pycom01g18540 pycom02g18780 pycom05g03650 pycom07g07790 pycom07g21680 pycom10g03590
rosa_chinensis RchiOBHm_Chr1g0344331 RchiOBHm_Chr1g0344461 RchiOBHm_Chr1g0344571 RchiOBHm_Chr1g0344661 RchiOBHm_Chr1g0344751 RchiOBHm_Chr1g0344811 RchiOBHm_Chr1g0344881 RchiOBHm_Chr1g0344901 RchiOBHm_Chr1g0344911 RchiOBHm_Chr1g0373091
rosa_laevigata RLG00000028867 RLG00000028877 RLG00000028880 RLG00000028885 RLG00000028898
rosa_multiflora Rmu_co8105290.1_g000001 Rmu_co8220756.1_g000001 Rmu_co8317965.1_g000001 Rmu_co8413861.1_g000001 Rmu_co8518347.1_g000001 Rmu_sc0000390.1_g000001 Rmu_sc0005177.1_g000002 Rmu_sc0007317.1_g000002 Rmu_sc0010876.1_g000003 Rmu_sc0013714.1_g000001 Rmu_sc0029558.1_g000002 Rmu_ssc0000167.1_g000004
rosa_roxburghii Rroxscaffold_4G00284470 Rroxscaffold_4G00309530 Rroxscaffold_4G00309580 Rroxscaffold_4G00309590 Rroxscaffold_4G00309620 Rroxscaffold_4G00309630 Rroxscaffold_4G00309720 Rroxscaffold_4G00309880
rosa_rugosa Rorug01G0173200 Rorug01G0173200 Rorug01G0174300 Rorug01G0174300 Rorug01G0174300 Rorug01G0174900 Rorug01G0376100
rosa_samantha Rh1CG175000 Rh1CG176200 Rh1CG177200 Rh1CG177800 Rh1CG177900 Rh1CG178000 Rh1CG361900 Rh1DG188500 Rh1DG188700 Rh1DG189400 Rh1DG379400
rosa_wichuraiana Rw1G015680 Rw1G015800 Rw1G015810 Rw1G015870 Rw1G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 701, 887
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 42
AccII CGCG 1 cut(s) 144
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 5 cut(s) 139, 186, 305, 477, 970
AfiI CCNNNNNNNGG 3 cut(s) 221, 779, 942
AgsI TTSAA 2 cut(s) 376, 882
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 3 cut(s) 241, 490, 603
AluBI AGCT 7 cut(s) 73, 237, 319, 670, 863, 948, 1105
AluI AGCT 7 cut(s) 73, 237, 319, 670, 863, 948, 1105
Alw21I GWGCWC 2 cut(s) 264, 321
Alw26I GTCTC 1 cut(s) 1127
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 1 cut(s) 771
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 4 cut(s) 22, 121, 266, 647
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 5 cut(s) 73, 117, 126, 635, 932
AspS9I GGNCC 2 cut(s) 245, 648
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 6 cut(s) 231, 292, 413, 506, 563, 1069
AsuII TTCGAA 2 cut(s) 597, 976
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 701, 887
BanII GRGCYC 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 1156
Bbv12I GWGCWC 2 cut(s) 264, 321
BbvI GCAGC 5 cut(s) 60, 104, 113, 647, 944
BccI CCATC 3 cut(s) 542, 557, 784
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 3 cut(s) 243, 492, 605
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 1127
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 1 cut(s) 35
BisI GCNGC 7 cut(s) 74, 118, 121, 124, 127, 636, 933
BlsI GCNGC 7 cut(s) 75, 119, 122, 125, 128, 637, 934
Bme1390I CCNGG 4 cut(s) 156, 243, 492, 605
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 245, 648
BmiI GGNNCC 2 cut(s) 703, 889
BmrFI CCNGG 4 cut(s) 156, 243, 492, 605
BmsI GCATC 2 cut(s) 339, 473
BpiI GAAGAC 1 cut(s) 1156
Bpu14I TTCGAA 2 cut(s) 597, 976
BpuMI CCSGG 1 cut(s) 156
BsaBI GATNNNNATC 2 cut(s) 95, 837
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 603, 604, 1080
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 779, 942
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 135, 714
Bse3DI GCAATG 1 cut(s) 1040
Bse8I GATNNNNATC 2 cut(s) 95, 837
BseBI CCWGG 3 cut(s) 243, 492, 605
BseDI CCNNGG 3 cut(s) 603, 604, 1080
BseGI GGATG 3 cut(s) 107, 488, 906
BseJI GATNNNNATC 2 cut(s) 95, 837
BseLI CCNNNNNNNGG 3 cut(s) 221, 779, 942
BseMI GCAATG 1 cut(s) 1040
BseNI ACTGG 2 cut(s) 135, 714
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 264
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 5 cut(s) 60, 104, 113, 647, 944
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 4 cut(s) 24, 123, 268, 649
BshNI GGYRCC 2 cut(s) 701, 887
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 264, 321
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 2 cut(s) 155, 222
BslFI GGGAC 2 cut(s) 182, 570
BslI CCNNNNNNNGG 3 cut(s) 221, 779, 942
BsmAI GTCTC 1 cut(s) 1127
BsmFI GGGAC 2 cut(s) 182, 570
BsnI GGCC 4 cut(s) 24, 123, 268, 649
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 2 cut(s) 597, 976
Bsp1286I GDGCHC 2 cut(s) 264, 321
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 3 cut(s) 282, 763, 873
Bsp19I CCATGG 1 cut(s) 1080
BspANI GGCC 4 cut(s) 24, 123, 268, 649
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 2 cut(s) 703, 889
BspPI GGATC 1 cut(s) 771
BspT104I TTCGAA 2 cut(s) 597, 976
BspT107I GGYRCC 2 cut(s) 701, 887
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 1040
BsrFI RCCGGY 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 2 cut(s) 135, 714
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 603, 604, 1080
BssMI GATC 3 cut(s) 282, 763, 873
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 1080
Bst2UI CCWGG 3 cut(s) 243, 492, 605
Bst4CI ACNGT 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 475
BstBI TTCGAA 2 cut(s) 597, 976
BstC8I GCNNGC 3 cut(s) 223, 401, 1024
BstDEI CTNAG 1 cut(s) 1007
BstDSI CCRYGG 1 cut(s) 1080
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 3 cut(s) 107, 488, 906
BstFNI CGCG 1 cut(s) 144
BstKTI GATC 3 cut(s) 285, 766, 876
BstMAI GTCTC 1 cut(s) 1127
BstMBI GATC 3 cut(s) 282, 763, 873
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 5 cut(s) 123, 126, 165, 259, 1111
BstNI CCWGG 3 cut(s) 243, 492, 605
BstNSI RCATGY 2 cut(s) 352, 403
BstSCI CCNGG 4 cut(s) 154, 241, 490, 603
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 5 cut(s) 60, 104, 113, 647, 944
BstV2I GAAGAC 1 cut(s) 1156
BstZI CGGCCG 1 cut(s) 121
BsuRI GGCC 4 cut(s) 24, 123, 268, 649
BtgI CCRYGG 1 cut(s) 1080
BtgZI GCGATG 1 cut(s) 752
BtsCI GGATG 3 cut(s) 107, 488, 906
BtsIMutI CAGTG 2 cut(s) 405, 829
Cac8I GCNNGC 3 cut(s) 223, 401, 1024
CciI TCATGA 1 cut(s) 363
CciNI GCGGCCGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 245, 648
CseI GACGC 2 cut(s) 133, 347
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 5 cut(s) 138, 185, 304, 476, 969
CviQI GTAC 5 cut(s) 138, 185, 304, 476, 969
DdeI CTNAG 1 cut(s) 1007
DpnI GATC 3 cut(s) 284, 765, 875
DpnII GATC 3 cut(s) 282, 763, 873
EaeI YGGCCR 1 cut(s) 121
EagI CGGCCG 1 cut(s) 121
EciI GGCGGA 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 319
EclXI CGGCCG 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 1080
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 241, 490, 603
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 1080
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 1080
FaqI GGGAC 2 cut(s) 182, 570
FauI CCCGC 3 cut(s) 149, 173, 281
FauNDI CATATG 1 cut(s) 940
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 7 cut(s) 74, 118, 121, 124, 127, 636, 933
FokI GGATG 3 cut(s) 94, 495, 893
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 7 cut(s) 74, 118, 121, 124, 127, 636, 933
FspBI CTAG 1 cut(s) 35
GluI GCNGC 7 cut(s) 74, 118, 121, 124, 127, 636, 933
HaeIII GGCC 4 cut(s) 24, 123, 268, 649
HapII CCGG 2 cut(s) 155, 222
HgaI GACGC 2 cut(s) 133, 347
Hin1I GRCGYC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 2 cut(s) 668, 1103
HinfI GANTC 5 cut(s) 389, 433, 497, 686, 1120
HpaII CCGG 2 cut(s) 155, 222
HphI GGTGA 6 cut(s) 231, 292, 413, 506, 563, 1069
Hpy166II GTNNAC 3 cut(s) 43, 262, 530
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 4 cut(s) 364, 383, 780, 919
Hpy8I GTNNAC 3 cut(s) 43, 262, 530
Hpy99I CGWCG 3 cut(s) 104, 149, 1071
HpyAV CCTTC 2 cut(s) 87, 268
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 2 cut(s) 258, 845
HpyCH4V TGCA 9 cut(s) 262, 330, 399, 635, 677, 729, 1026, 1040, 1114
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 126, 165, 259, 1111
HpyF3I CTNAG 1 cut(s) 1007
HpySE526I ACGT 2 cut(s) 258, 845
Hsp92I GRCGYC 1 cut(s) 339
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Kzo9I GATC 3 cut(s) 282, 763, 873
LmnI GCTCC 2 cut(s) 173, 945
Lsp1109I GCAGC 5 cut(s) 60, 104, 113, 647, 944
LweI GCATC 2 cut(s) 339, 473
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 258, 845
MaeIII GTNAC 1 cut(s) 494
MalI GATC 3 cut(s) 284, 765, 875
MbiI CCGCTC 1 cut(s) 168
MboI GATC 3 cut(s) 282, 763, 873
MboII GAAGA 5 cut(s) 283, 398, 422, 425, 1161
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 2 cut(s) 264, 321
MluCI AATT 4 cut(s) 13, 639, 678, 955
MlyI GAGTC 2 cut(s) 491, 1129
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 8 cut(s) 14, 160, 258, 303, 371, 754, 783, 1132
MroNI GCCGGC 1 cut(s) 221
MseI TTAA 2 cut(s) 1056, 1162
MslI CAYNNNNRTG 2 cut(s) 398, 455
MspA1I CMGCKG 3 cut(s) 73, 117, 126
MspI CCGG 2 cut(s) 155, 222
MspR9I CCNGG 4 cut(s) 156, 243, 492, 605
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 3 cut(s) 243, 492, 605
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 5 cut(s) 123, 126, 165, 259, 1111
NaeI GCCGGC 1 cut(s) 223
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 1080
NdeI CATATG 1 cut(s) 940
NdeII GATC 3 cut(s) 282, 763, 873
NgoMIV GCCGGC 1 cut(s) 221
NlaIV GGNNCC 2 cut(s) 703, 889
NmuCI GTSAC 1 cut(s) 494
NotI GCGGCCGC 1 cut(s) 121
NspI RCATGY 2 cut(s) 352, 403
NspV TTCGAA 2 cut(s) 597, 976
PaeI GCATGC 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 307
PagI TCATGA 1 cut(s) 363
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 389, 433, 686
PkrI GCNGC 7 cut(s) 75, 119, 122, 125, 128, 637, 934
PleI GAGTC 2 cut(s) 491, 1128
PpsI GAGTC 2 cut(s) 491, 1128
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 241, 490, 603
PspGI CCWGG 3 cut(s) 241, 490, 603
PspN4I GGNNCC 2 cut(s) 703, 889
PspPI GGNCC 2 cut(s) 245, 648
PspPPI RGGWCCY 1 cut(s) 245
PspXI VCTCGAGB 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 5 cut(s) 139, 186, 305, 477, 970
RsaNI GTAC 5 cut(s) 138, 185, 304, 476, 969
RseI CAYNNNNRTG 2 cut(s) 398, 455
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 1056, 1162
SatI GCNGC 7 cut(s) 74, 118, 121, 124, 127, 636, 933
Sau3AI GATC 3 cut(s) 282, 763, 873
Sau96I GGNCC 2 cut(s) 245, 648
SchI GAGTC 2 cut(s) 491, 1129
ScrFI CCNGG 4 cut(s) 156, 243, 492, 605
SduI GDGCHC 2 cut(s) 264, 321
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 2 cut(s) 339, 473
Sfr274I CTCGAG 1 cut(s) 307
SfuI TTCGAA 2 cut(s) 597, 976
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 2 cut(s) 398, 455
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 1 cut(s) 403
Sse9I AATT 4 cut(s) 13, 639, 678, 955
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 4 cut(s) 154, 241, 490, 603
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 2 cut(s) 261, 848
TaqI TCGA 5 cut(s) 42, 308, 597, 918, 976
TasI AATT 4 cut(s) 13, 639, 678, 955
TatI WGTACW 1 cut(s) 475
TauI GCSGC 2 cut(s) 123, 126
TfiI GAWTC 3 cut(s) 389, 433, 686
Tru1I TTAA 2 cut(s) 1056, 1162
Tru9I TTAA 2 cut(s) 1056, 1162
TscAI CASTG 2 cut(s) 412, 829
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 5 cut(s) 73, 117, 126, 635, 932
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 6 cut(s) 570, 588, 662, 749, 895, 1097
TspGWI ACGGA 2 cut(s) 100, 1161
TspRI CASTG 2 cut(s) 412, 829
VneI GTGCAC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 777
XceI RCATGY 2 cut(s) 352, 403
XcmI CCANNNNNNNNNTGG 2 cut(s) 783, 979
XhoI CTCGAG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.