MD00G1036700.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000663
Physical Location & Seq
Reverse (-)
6488000 .. 6491522
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1036700.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGTGTAGCAACCTTGTAAGCAGGGTGTTGATATTGGCGTGGCTGTTGGCGATAGCCACAGCACAAACCTTTGCTTCCCAAAACAACTACAAGGATGCTTTGTCGAAGTGCCTTTTATTTTTTGAGGGGCAAAGGTCAGGAAAGTTACCACCTTCGCAACGACTAACTTGGAGAAAAGATTCTGCCCTAAAAGATGGCTCTGATATTGGCAGGGATCTTGTAGGAGGGTACTATGATGCAGGTGACAATGTGAAGTTCAACTTTCCGATGGCGTTCACTACAACGATGTTGTCATGGAGCGTTCTAGAGTTCGGAAATTCGATGGGTGCAGATCATGAACATGCTTTGGAAGCTATAAGATGGGGGACTGACTATTTTCTTAAAGCTACTAGTGTTCCTGATTCTGTCGTTGGTGCAGTTGGTGACCCCAATGGCGACCACACTTGTTGGCAAAGGCCAGAAGACATGGACACTCCTAGAACCTCTTATGTGGTAACAAAAGAAAAGCCAGGTTCTGAGGTATCGGCCGAGATTGCAGCTGCGCTTGCAGCGTCTTCCATGGTTTTTAAAGATTCAGACAAAGGCTACTCTGCTTTGCTTCTTAAACGATCCATCGAGGTTTTTGAATTTGCAGATAAGTACAAGGGCTCTTACGGTGACAATATTGGTAGCGGAGTGTGTCCTTTTTACTGTGATTTTAGCGGCTACATGGATGAATTGGTTTGGGGAGCTGCGTGGTTATACAAGGCAACCAATACACCAGATTATTGGAACTATGTCAGAAACAACATAGTTGGTGATGTCACTGAATTTGGGTGGGATTCAAAACATGCTGGCATCAACGTACTCGTTTCTCAGTGGGCAATGACTGATCCCAATAATTCTAATCCCTTTATTCCCAAGGCAGACCAACTTGTATGCTCCATTTTGCCTGAATCACCTGCTCAAAAATCAGTCACATATTCACCAGGTGGGCTCTTGTTCAAACCTGGTGGAAGTAACCTCCAACATACAACGTCCCTATCATTTCTTCTTTCAGTTTATGCAGGCTACATGAAAGCTAACAACAAAGTTATCGACTGTGGCAACAATGTTGTTGTCACTCCAGCTAAATTGGTGGATTTCACCAAAGGCCAGGTTGATTATATACTAGGAAAAAACCCACTAGGGATGTCATACATGGTTGGATTTGGGCAAAAGTTTCCTCAGAAAATACATCACCGTGGGTCTGTGATACCTTCGATCGACCAACACCCGCAACATATCGAGTGCCATGGAGGAGACGAGTATTTCAAGAGCAACAGTCCTAACCCTAACTTGCTAACAGGTGCTATTGTGGGAGGACCTGCTGAAAACGATACATTCGAAGATTCTCGATCTAATGTTGCACAATCGGAGCCAACCACATATATCAACGCACCTTTTGTGGGTGTATTGGCTGCCTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

484

Amino Acids

52.75

Weight (kDa)

5.41

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 479 2.5e-152 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 230, 949
Acc36I ACCTGC 3 cut(s) 230, 949, 1354
AciI CCGC 3 cut(s) 672, 702, 1256
AclWI GGATC 3 cut(s) 222, 603, 866
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 3 cut(s) 316, 626, 809
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 3 cut(s) 230, 641, 846
AfiI CCNNNNNNNGG 1 cut(s) 1427
AgsI TTSAA 5 cut(s) 259, 626, 825, 985, 1294
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 4 cut(s) 508, 967, 988, 1134
AluBI AGCT 6 cut(s) 353, 386, 539, 731, 1061, 1109
AluI AGCT 6 cut(s) 353, 386, 539, 731, 1061, 1109
Alw26I GTCTC 1 cut(s) 1275
AlwI GGATC 3 cut(s) 222, 603, 866
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 3 cut(s) 455, 525, 1132
ApeKI GCWGC 5 cut(s) 536, 539, 548, 731, 1439
ApoI RAATTY 3 cut(s) 316, 626, 809
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 1 cut(s) 1343
AsuHPI GGTGA 8 cut(s) 254, 434, 668, 809, 930, 957, 1117, 1211
AsuII TTCGAA 1 cut(s) 1365
AvaII GGWCC 1 cut(s) 1343
BanII GRGCYC 2 cut(s) 650, 978
BbsI GAAGAC 2 cut(s) 468, 546
BbvI GCAGC 5 cut(s) 526, 548, 560, 718, 1426
BccI CCATC 5 cut(s) 188, 262, 316, 354, 620
BciT130I CCWGG 4 cut(s) 510, 969, 990, 1136
BcoDI GTCTC 1 cut(s) 1275
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 5 cut(s) 305, 390, 477, 1151, 1166
BfuAI ACCTGC 3 cut(s) 230, 949, 1354
BisI GCNGC 6 cut(s) 537, 540, 549, 703, 732, 1440
BlsI GCNGC 6 cut(s) 538, 541, 550, 704, 733, 1441
Bme1390I CCNGG 4 cut(s) 510, 969, 990, 1136
Bme18I GGWCC 1 cut(s) 1343
BmgT120I GGNCC 1 cut(s) 1343
BmiI GGNNCC 1 cut(s) 1398
BmrFI CCNGG 4 cut(s) 510, 969, 990, 1136
BmsI GCATC 3 cut(s) 85, 226, 846
BpiI GAAGAC 2 cut(s) 468, 546
BpmI CTGGAG 1 cut(s) 1089
Bpu14I TTCGAA 1 cut(s) 1365
BsaJI CCNNGG 4 cut(s) 558, 900, 1222, 1273
Bsc4I CCNNNNNNNGG 1 cut(s) 1427
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 4 cut(s) 510, 969, 990, 1136
BseDI CCNNGG 4 cut(s) 558, 900, 1222, 1273
BseGI GGATG 3 cut(s) 100, 718, 1176
BseLI CCNNNNNNNGG 1 cut(s) 1427
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 3 cut(s) 507, 869, 1220
BseRI GAGGAG 1 cut(s) 1293
BseX3I CGGCCG 1 cut(s) 525
BseXI GCAGC 5 cut(s) 526, 548, 560, 718, 1426
BsgI GTGCAG 2 cut(s) 348, 435
Bsh1285I CGRYCG 2 cut(s) 528, 1245
BshFI GGCC 3 cut(s) 457, 527, 1134
BsiEI CGRYCG 2 cut(s) 528, 1245
BslFI GGGAC 2 cut(s) 379, 1003
BslI CCNNNNNNNGG 1 cut(s) 1427
BsmAI GTCTC 1 cut(s) 1275
BsmBI CGTCTC 1 cut(s) 1275
BsmFI GGGAC 2 cut(s) 379, 1003
BsnI GGCC 3 cut(s) 457, 527, 1134
Bsp119I TTCGAA 1 cut(s) 1365
Bsp1286I GDGCHC 2 cut(s) 650, 978
Bsp143I GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
Bsp19I CCATGG 2 cut(s) 558, 1273
BspACI CCGC 3 cut(s) 672, 702, 1256
BspANI GGCC 3 cut(s) 457, 527, 1134
BspCNI CTCAG 3 cut(s) 508, 868, 1219
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 1398
BspMI ACCTGC 3 cut(s) 230, 949, 1354
BspPI GGATC 3 cut(s) 222, 603, 866
BspT104I TTCGAA 1 cut(s) 1365
BsrDI GCAATG 1 cut(s) 870
BssECI CCNNGG 4 cut(s) 558, 900, 1222, 1273
BssMI GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
BssT1I CCWWGG 3 cut(s) 558, 900, 1273
Bst2UI CCWGG 4 cut(s) 510, 969, 990, 1136
Bst4CI ACNGT 5 cut(s) 656, 692, 1082, 1223, 1304
BstBI TTCGAA 1 cut(s) 1365
BstC8I GCNNGC 3 cut(s) 546, 835, 1048
BstDEI CTNAG 3 cut(s) 516, 855, 1206
BstDSI CCRYGG 3 cut(s) 558, 1222, 1273
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 100, 718, 1176
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 6 cut(s) 217, 334, 611, 874, 1245, 1379
BstMAI GTCTC 1 cut(s) 1275
BstMBI GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
BstMCI CGRYCG 2 cut(s) 528, 1245
BstMWI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
BstNI CCWGG 4 cut(s) 510, 969, 990, 1136
BstNSI RCATGY 2 cut(s) 344, 833
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 508, 967, 988, 1134
BstV1I GCAGC 5 cut(s) 526, 548, 560, 718, 1426
BstV2I GAAGAC 2 cut(s) 468, 546
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 768
BstYI RGATCY 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 525
BsuRI GGCC 3 cut(s) 457, 527, 1134
BtgI CCRYGG 3 cut(s) 558, 1222, 1273
BtsCI GGATG 3 cut(s) 100, 718, 1176
BtsIMutI CAGTG 2 cut(s) 804, 863
BveI ACCTGC 3 cut(s) 230, 949, 1354
Cac8I GCNNGC 3 cut(s) 546, 835, 1048
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 1343
CseI GACGC 1 cut(s) 540
CsiI ACCWGGT 2 cut(s) 967, 988
Csp6I GTAC 3 cut(s) 229, 640, 845
CspCI CAANNNNNGTGG 4 cut(s) 428, 463, 973, 1008
CviQI GTAC 3 cut(s) 229, 640, 845
DdeI CTNAG 3 cut(s) 516, 855, 1206
DpnI GATC 6 cut(s) 216, 333, 610, 873, 1244, 1378
DpnII GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
DraI TTTAAA 1 cut(s) 568
DraIII CACNNNGTG 1 cut(s) 971
EaeI YGGCCR 1 cut(s) 525
EagI CGGCCG 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 525
Eco130I CCWWGG 3 cut(s) 558, 900, 1273
Eco24I GRGCYC 2 cut(s) 650, 978
Eco47I GGWCC 1 cut(s) 1343
Eco52I CGGCCG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 1343
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 4 cut(s) 508, 967, 988, 1134
EcoT14I CCWWGG 3 cut(s) 558, 900, 1273
EcoT38I GRGCYC 2 cut(s) 650, 978
ErhI CCWWGG 3 cut(s) 558, 900, 1273
Esp3I CGTCTC 1 cut(s) 1275
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 379, 1003
FauI CCCGC 1 cut(s) 1263
Fnu4HI GCNGC 6 cut(s) 537, 540, 549, 703, 732, 1440
FokI GGATG 3 cut(s) 107, 725, 1183
FriOI GRGCYC 2 cut(s) 650, 978
Fsp4HI GCNGC 6 cut(s) 537, 540, 549, 703, 732, 1440
FspBI CTAG 5 cut(s) 305, 390, 477, 1151, 1166
GlaI GCGC 1 cut(s) 543
GluI GCNGC 6 cut(s) 537, 540, 549, 703, 732, 1440
GsuI CTGGAG 1 cut(s) 1089
HaeIII GGCC 3 cut(s) 457, 527, 1134
HgaI GACGC 1 cut(s) 540
HhaI GCGC 1 cut(s) 544
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 6 cut(s) 179, 401, 572, 821, 935, 1370
HphI GGTGA 8 cut(s) 254, 434, 668, 809, 930, 957, 1117, 1211
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 8 cut(s) 202, 267, 314, 517, 577, 782, 1209, 1396
Hpy188III TCNNGA 6 cut(s) 138, 305, 335, 398, 1294, 1374
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 162, 1248
HpyCH4III ACNGT 5 cut(s) 656, 692, 1082, 1223, 1304
HpyCH4IV ACGT 2 cut(s) 843, 1016
HpyCH4V TGCA 8 cut(s) 239, 329, 416, 536, 548, 632, 1046, 1388
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
HpyF3I CTNAG 3 cut(s) 516, 855, 1206
HpySE526I ACGT 2 cut(s) 843, 1016
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
LmnI GCTCC 4 cut(s) 297, 728, 926, 1396
Lsp1109I GCAGC 5 cut(s) 526, 548, 560, 718, 1426
LweI GCATC 3 cut(s) 85, 226, 846
MabI ACCWGGT 2 cut(s) 967, 988
MaeI CTAG 5 cut(s) 305, 390, 477, 1151, 1166
MaeII ACGT 2 cut(s) 843, 1016
MaeIII GTNAC 9 cut(s) 144, 242, 422, 493, 656, 802, 955, 998, 1099
MalI GATC 6 cut(s) 216, 333, 610, 873, 1244, 1378
MboI GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
MboII GAAGA 4 cut(s) 473, 546, 1022, 1379
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 650, 978
MluCI AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
MmeI TCCRAC 2 cut(s) 1030, 1165
MnlI CCTC 9 cut(s) 118, 218, 493, 511, 610, 1013, 1215, 1271, 1334
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 4 cut(s) 381, 567, 603, 1450
MslI CAYNNNNRTG 2 cut(s) 339, 1221
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 4 cut(s) 510, 969, 990, 1136
MvaI CCWGG 4 cut(s) 510, 969, 990, 1136
MwoI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
NcoI CCATGG 2 cut(s) 558, 1273
NdeII GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
NlaIV GGNNCC 1 cut(s) 1398
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
NspI RCATGY 2 cut(s) 344, 833
NspV TTCGAA 1 cut(s) 1365
PagI TCATGA 1 cut(s) 334
PaqCI CACCTGC 2 cut(s) 230, 949
PcsI WCGNNNNNNNCGW 1 cut(s) 1362
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 6 cut(s) 179, 401, 572, 821, 935, 1370
PkrI GCNGC 6 cut(s) 538, 541, 550, 704, 733, 1441
Ple19I CGATCG 1 cut(s) 1245
PpuMI RGGWCCY 1 cut(s) 1343
Psp5II RGGWCCY 1 cut(s) 1343
Psp6I CCWGG 4 cut(s) 508, 967, 988, 1134
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 4 cut(s) 508, 967, 988, 1134
PspN4I GGNNCC 1 cut(s) 1398
PspPI GGNCC 1 cut(s) 1343
PspPPI RGGWCCY 1 cut(s) 1343
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 214
PvuI CGATCG 1 cut(s) 1245
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 3 cut(s) 230, 641, 846
RsaNI GTAC 3 cut(s) 229, 640, 845
RseI CAYNNNNRTG 2 cut(s) 339, 1221
SaqAI TTAA 4 cut(s) 381, 567, 603, 1450
SatI GCNGC 6 cut(s) 537, 540, 549, 703, 732, 1440
Sau3AI GATC 6 cut(s) 214, 331, 608, 871, 1242, 1376
Sau96I GGNCC 1 cut(s) 1343
ScrFI CCNGG 4 cut(s) 510, 969, 990, 1136
SduI GDGCHC 2 cut(s) 650, 978
SexAI ACCWGGT 2 cut(s) 967, 988
SfaNI GCATC 3 cut(s) 85, 226, 846
SfuI TTCGAA 1 cut(s) 1365
SinI GGWCC 1 cut(s) 1343
SmiMI CAYNNNNRTG 2 cut(s) 339, 1221
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
SsiI CCGC 3 cut(s) 672, 702, 1256
SspI AATATT 1 cut(s) 664
SspMI CTAG 5 cut(s) 305, 390, 477, 1151, 1166
StyD4I CCNGG 4 cut(s) 508, 967, 988, 1134
StyI CCWWGG 3 cut(s) 558, 900, 1273
TaaI ACNGT 5 cut(s) 656, 692, 1082, 1223, 1304
TaiI ACGT 2 cut(s) 846, 1019
TaqI TCGA 9 cut(s) 104, 320, 615, 1077, 1241, 1245, 1266, 1365, 1375
TasI AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
TatI WGTACW 1 cut(s) 639
TauI GCSGC 1 cut(s) 705
TfiI GAWTC 6 cut(s) 179, 401, 572, 821, 935, 1370
Tru1I TTAA 4 cut(s) 381, 567, 603, 1450
Tru9I TTAA 4 cut(s) 381, 567, 603, 1450
TscAI CASTG 2 cut(s) 811, 863
TseFI GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
TseI GCWGC 5 cut(s) 536, 539, 548, 731, 1439
Tsp45I GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
TspDTI ATGAA 3 cut(s) 351, 729, 1070
TspRI CASTG 2 cut(s) 811, 863
VpaK11BI GGWCC 1 cut(s) 1343
XapI RAATTY 3 cut(s) 316, 626, 809
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 2 cut(s) 344, 833
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 5 cut(s) 305, 390, 477, 1151, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.