RchiOBHm_Chr1g0374591

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000663
Physical Location & Seq
Forward (+)
62373668 .. 62376085
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59839

Sequence Viewer

Length: 1479 bp
ATGTGTACCAATTTCCTTCTATTAAGGCTGTTGATAGCAGCAGGGTTGTTGGCAATGGCAACCGCCGCAAAAGTGACTCCACATAACTACGCAGATGCTCTGTCGAAGTCCATTTTATTTTTTGAAGGCCAGAGATCTGGCAAGTTACCGAATTCTCAACGAATGAATTGGAGGAAAGATTCTGCCCTACATGATGGCAGCGATGTTCATAGGGATCTGGTGGGGGGTTACCATGATGCTGGTGACAATGTGAAGTTCAACTTTCCCATGGCCTTCACAACAACATTGTTGTCATGGAGCGTTCTAGAGTTTGGAGATTTGATGGGATCTGAACTTCAACATGCTTTGGAAGCTATAAGATGGGCAACTGATTATTTTCTTAAAGCCACAAATGATCCTGGTTCAGTAGTTGGCGTAGTAGGCGACCCCAACGGAGATCATGCTTGTTGGCAAAGGCCTGAAGACCTGGATACCCCAAGAACCACATATGTGGTAACTAAAGAAAAACCAGGTTCAGAAGTTTCTGCGGAGATTGCAGCCATGCTTGCAGCTTCCTCTATAGTGTTTAACCAATCAGACAAAGCCTACTCTGACTCGCTTCTTAAGCGAGCCATCCAAGTGTTTCAATTTGCAGATCAGTATCAGGGATCTTATAATACTAGTATTGGAGACGGAGCCTGCCCATTTTACTGCGATTTTAGTGGCTACATGCTATACAAGGCAACAAAGGAACCAAATTATTGGAAATATGTCAAAGACCACATACACAATCTGGAGTCGTCAAACACTGTAGTGAGAAATGTGGATGGTTCTCCTGTTCCATTCGGATGGGATAGCATGCATGCCGGCATCAACATACTTGTTTCTCAATGGGTTATGACTGATCCAGCCAATTCCAATCCGTTCATTCCCAAAGCTGACCAGCTAATATGCTCCATTCTGCCTGAATCACCAGCTCCCAAATCAGTCACGTATTCACCAGGTGGACTCTTGTTCAAACCTGGATGTTGTAACCTGCAACACATGACATCCTTATCATTTCTTCTTATAGTTTATGCTCGATACCTGAAAGGTGCCAACAGAACCATAAACTGTGGCAATAATGTTGTTCTTGGTCCAGCTCATTTGATAAATTTCACAAGAAAACAGGTTGATTATATACTCGGAAGCAACCCATTGGGGATGTCTTATATGGTGGGATATGGTACAAAATTTCCTCAAAGAATACATCATCGTGCTTCTGTGGTACCTTCCATGGATAAGCATCCACAGCACATGAGGTGCCATGACGGTGATCAGTATTTCAAAAGCCAAGCTCCGAATCAAAACTTGCTAATAGGAGCAGTTGTCGGGGGACCAGCCGAGAATGATACATTTGAAGATTCTCGATATGATCCTGGACAATCAGAGCCAGTTACATACAACAATGCCCCTTTTGTTGGTGTTTTGGCATTCTTTAAGGCTCTATATAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

492

Amino Acids

54.33

Weight (kDa)

6.34

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 483 3.3e-137 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
Acc36I ACCTGC 1 cut(s) 1023
Acc65I GGTACC 1 cut(s) 1246
AccB1I GGYRCC 3 cut(s) 1073, 1246, 1281
AciI CCGC 3 cut(s) 63, 66, 527
AclWI GGATC 6 cut(s) 222, 334, 389, 655, 878, 1388
AcsI RAATTY 3 cut(s) 151, 1132, 1211
AcuI CTGAAG 1 cut(s) 480
AdeI CACNNNGTG 1 cut(s) 983
AfaI GTAC 3 cut(s) 7, 1207, 1248
AfiI CCNNNNNNNGG 1 cut(s) 1439
AflII CTTAAG 1 cut(s) 602
AgsI TTSAA 7 cut(s) 125, 259, 338, 626, 997, 1306, 1379
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
AleI CACNNNNGTG 2 cut(s) 488, 791
AluBI AGCT 7 cut(s) 353, 551, 917, 925, 956, 1121, 1316
AluI AGCT 7 cut(s) 353, 551, 917, 925, 956, 1121, 1316
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 6 cut(s) 222, 334, 389, 655, 878, 1388
AoxI GGCC 3 cut(s) 127, 270, 455
ApeKI GCWGC 4 cut(s) 38, 198, 536, 548
ApoI RAATTY 3 cut(s) 151, 1132, 1211
Asp718I GGTACC 1 cut(s) 1246
AspS9I GGNCC 2 cut(s) 1115, 1355
AsuHPI GGTGA 4 cut(s) 254, 942, 969, 1304
AvaII GGWCC 2 cut(s) 1115, 1355
BanI GGYRCC 3 cut(s) 1073, 1246, 1281
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 4 cut(s) 50, 210, 548, 560
BccI CCATC 6 cut(s) 188, 316, 354, 620, 800, 822
BciT130I CCWGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
BciVI GTATCC 1 cut(s) 463
BclI TGATCA 1 cut(s) 1294
BcoDI GTCTC 1 cut(s) 663
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 2 cut(s) 305, 660
BfmI CTRYAG 2 cut(s) 558, 789
BfrI CTTAAG 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 1023
BfuI GTATCC 1 cut(s) 463
BglII AGATCT 1 cut(s) 134
BisI GCNGC 5 cut(s) 39, 66, 199, 537, 549
BlsI GCNGC 5 cut(s) 40, 67, 200, 538, 550
Bme1390I CCNGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
Bme18I GGWCC 2 cut(s) 1115, 1355
BmgT120I GGNCC 2 cut(s) 1115, 1355
BmiI GGNNCC 6 cut(s) 676, 732, 1075, 1248, 1283, 1356
BmrFI CCNGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
BmsI GCATC 4 cut(s) 85, 226, 858, 1273
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 794
BsaAI YACGTR 1 cut(s) 972
BsaBI GATNNNNATC 2 cut(s) 639, 1263
BsaJI CCNNGG 2 cut(s) 267, 1254
BsaXI ACNNNNNCTCC 2 cut(s) 1332, 1362
Bsc4I CCNNNNNNNGG 1 cut(s) 1439
Bse118I RCCGGY 1 cut(s) 845
Bse1I ACTGG 1 cut(s) 1412
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 2 cut(s) 639, 1263
BseBI CCWGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
BseDI CCNNGG 2 cut(s) 267, 1254
BseGI GGATG 7 cut(s) 612, 811, 833, 1010, 1028, 1188, 1264
BseJI GATNNNNATC 2 cut(s) 639, 1263
BseLI CCNNNNNNNGG 1 cut(s) 1439
BseMI GCAATG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 1412
BseXI GCAGC 4 cut(s) 50, 210, 548, 560
BshFI GGCC 3 cut(s) 129, 272, 457
BshNI GGYRCC 3 cut(s) 1073, 1246, 1281
BsiSI CCGG 1 cut(s) 846
BslFI GGGAC 1 cut(s) 1368
BslI CCNNNNNNNGG 1 cut(s) 1439
BsmAI GTCTC 1 cut(s) 663
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 1 cut(s) 1368
BsmI GAATGC 1 cut(s) 1451
BsnI GGCC 3 cut(s) 129, 272, 457
Bsp19I CCATGG 2 cut(s) 267, 1254
BspACI CCGC 3 cut(s) 63, 66, 527
BspANI GGCC 3 cut(s) 129, 272, 457
BspLI GGNNCC 6 cut(s) 676, 732, 1075, 1248, 1283, 1356
BspMI ACCTGC 1 cut(s) 1023
BspPI GGATC 6 cut(s) 222, 334, 389, 655, 878, 1388
BspT107I GGYRCC 3 cut(s) 1073, 1246, 1281
BspTI CTTAAG 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 845
BsrI ACTGG 1 cut(s) 1412
BssAI RCCGGY 1 cut(s) 845
BssECI CCNNGG 2 cut(s) 267, 1254
BssT1I CCWWGG 2 cut(s) 267, 1254
Bst2UI CCWGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
Bst4CI ACNGT 3 cut(s) 790, 1094, 1292
BstAFI CTTAAG 1 cut(s) 602
BstBAI YACGTR 1 cut(s) 972
BstC8I GCNNGC 6 cut(s) 546, 609, 679, 839, 843, 847
BstDSI CCRYGG 2 cut(s) 267, 1254
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 7 cut(s) 612, 811, 833, 1010, 1028, 1188, 1264
BstMAI GTCTC 1 cut(s) 663
BstMWI GCNNNNNNNGC 7 cut(s) 65, 350, 420, 533, 545, 604, 1270
BstNI CCWGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
BstNSI RCATGY 4 cut(s) 344, 712, 841, 845
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
BstSFI CTRYAG 2 cut(s) 558, 789
BstV1I GCAGC 4 cut(s) 50, 210, 548, 560
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 4 cut(s) 134, 214, 326, 647
BstXI CCANNNNNNTGG 5 cut(s) 137, 239, 490, 741, 828
BstYI RGATCY 4 cut(s) 134, 214, 326, 647
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 3 cut(s) 129, 272, 457
BtgI CCRYGG 2 cut(s) 267, 1254
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 7 cut(s) 612, 811, 833, 1010, 1028, 1188, 1264
BtsIMutI CAGTG 1 cut(s) 786
BveI ACCTGC 1 cut(s) 1023
Cac8I GCNNGC 6 cut(s) 546, 609, 679, 839, 843, 847
Cfr10I RCCGGY 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 1115, 1355
CsiI ACCWGGT 2 cut(s) 508, 979
Csp6I GTAC 3 cut(s) 6, 1206, 1247
CviQI GTAC 3 cut(s) 6, 1206, 1247
DraIII CACNNNGTG 1 cut(s) 983
Eco130I CCWWGG 2 cut(s) 267, 1254
Eco147I AGGCCT 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 1115, 1355
Eco57I CTGAAG 1 cut(s) 480
Eco91I GGTNACC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
EcoT14I CCWWGG 2 cut(s) 267, 1254
EcoT22I ATGCAT 1 cut(s) 843
ErhI CCWWGG 2 cut(s) 267, 1254
Esp3I CGTCTC 1 cut(s) 663
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 1368
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 1 cut(s) 1294
Fnu4HI GCNGC 5 cut(s) 39, 66, 199, 537, 549
FokI GGATG 7 cut(s) 599, 818, 840, 1015, 1017, 1195, 1251
Fsp4HI GCNGC 5 cut(s) 39, 66, 199, 537, 549
FspBI CTAG 2 cut(s) 305, 660
GluI GCNGC 5 cut(s) 39, 66, 199, 537, 549
GsuI CTGGAG 1 cut(s) 794
HaeIII GGCC 3 cut(s) 129, 272, 457
HapII CCGG 1 cut(s) 846
HinfI GANTC 8 cut(s) 76, 179, 593, 776, 947, 987, 1321, 1382
HpaII CCGG 1 cut(s) 846
HphI GGTGA 4 cut(s) 254, 942, 969, 1304
Hpy166II GTNNAC 2 cut(s) 6, 986
Hpy188I TCNGA 8 cut(s) 331, 517, 577, 592, 827, 1166, 1320, 1408
Hpy188III TCNNGA 3 cut(s) 305, 773, 1386
Hpy8I GTNNAC 2 cut(s) 6, 986
HpyAV CCTTC 4 cut(s) 26, 119, 283, 1260
HpyCH4III ACNGT 3 cut(s) 790, 1094, 1292
HpyCH4IV ACGT 1 cut(s) 971
HpyCH4V TGCA 5 cut(s) 536, 548, 632, 841, 1018
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 350, 420, 533, 545, 604, 1270
HpySE526I ACGT 1 cut(s) 971
KpnI GGTACC 1 cut(s) 1250
KroI GCCGGC 1 cut(s) 845
KroNI GCCGGC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 1294
LmnI GCTCC 6 cut(s) 297, 674, 938, 961, 1321, 1340
Lsp1109I GCAGC 4 cut(s) 50, 210, 548, 560
LweI GCATC 4 cut(s) 85, 226, 858, 1273
MabI ACCWGGT 2 cut(s) 508, 979
MaeI CTAG 2 cut(s) 305, 660
MaeII ACGT 1 cut(s) 971
MaeIII GTNAC 8 cut(s) 73, 144, 227, 242, 493, 967, 1010, 1414
MboII GAAGA 3 cut(s) 473, 1034, 1391
MflI RGATCY 4 cut(s) 134, 214, 326, 647
MluCI AATT 9 cut(s) 10, 151, 166, 626, 736, 892, 1132, 1211, 1471
MlyI GAGTC 4 cut(s) 70, 587, 785, 981
MnlI CCTC 4 cut(s) 165, 565, 1227, 1272
Mph1103I ATGCAT 1 cut(s) 843
MroNI GCCGGC 1 cut(s) 845
MseI TTAA 5 cut(s) 23, 381, 567, 603, 1458
MslI CAYNNNNRTG 6 cut(s) 488, 617, 791, 826, 1233, 1290
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 1 cut(s) 846
MspR9I CCNGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
Mva1269I GAATGC 1 cut(s) 1451
MvaI CCWGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
MwoI GCNNNNNNNGC 7 cut(s) 65, 350, 420, 533, 545, 604, 1270
NaeI GCCGGC 1 cut(s) 847
NcoI CCATGG 2 cut(s) 267, 1254
NdeI CATATG 1 cut(s) 487
NgoMIV GCCGGC 1 cut(s) 845
NlaIV GGNNCC 6 cut(s) 676, 732, 1075, 1248, 1283, 1356
NmeAIII GCCGAG 1 cut(s) 1387
NmuCI GTSAC 3 cut(s) 73, 242, 967
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 4 cut(s) 344, 712, 841, 845
OliI CACNNNNGTG 2 cut(s) 488, 791
PaeI GCATGC 2 cut(s) 841, 845
PceI AGGCCT 1 cut(s) 457
PctI GAATGC 1 cut(s) 1451
PdiI GCCGGC 1 cut(s) 847
PfeI GAWTC 4 cut(s) 179, 947, 1321, 1382
PfoI TCCNGGA 1 cut(s) 1396
PkrI GCNGC 5 cut(s) 40, 67, 200, 538, 550
PleI GAGTC 4 cut(s) 70, 587, 784, 981
PpsI GAGTC 4 cut(s) 70, 587, 784, 981
Ppu21I YACGTR 1 cut(s) 972
PsiI TTATAA 1 cut(s) 654
Psp6I CCWGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
PspEI GGTNACC 1 cut(s) 227
PspGI CCWGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
PspN4I GGNNCC 6 cut(s) 676, 732, 1075, 1248, 1283, 1356
PspPI GGNCC 2 cut(s) 1115, 1355
PsuI RGATCY 4 cut(s) 134, 214, 326, 647
RsaI GTAC 3 cut(s) 7, 1207, 1248
RsaNI GTAC 3 cut(s) 6, 1206, 1247
RseI CAYNNNNRTG 6 cut(s) 488, 617, 791, 826, 1233, 1290
SaqAI TTAA 5 cut(s) 23, 381, 567, 603, 1458
SatI GCNGC 5 cut(s) 39, 66, 199, 537, 549
Sau96I GGNCC 2 cut(s) 1115, 1355
SchI GAGTC 4 cut(s) 70, 587, 785, 981
ScrFI CCNGG 6 cut(s) 399, 467, 510, 981, 1002, 1398
SexAI ACCWGGT 2 cut(s) 508, 979
SfaNI GCATC 4 cut(s) 85, 226, 858, 1273
SfcI CTRYAG 2 cut(s) 558, 789
SinI GGWCC 2 cut(s) 1115, 1355
SmiMI CAYNNNNRTG 6 cut(s) 488, 617, 791, 826, 1233, 1290
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SpeI ACTAGT 1 cut(s) 659
SphI GCATGC 2 cut(s) 841, 845
Sse9I AATT 9 cut(s) 10, 151, 166, 626, 736, 892, 1132, 1211, 1471
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 3 cut(s) 63, 66, 527
SspMI CTAG 2 cut(s) 305, 660
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 6 cut(s) 397, 465, 508, 979, 1000, 1396
StyI CCWWGG 2 cut(s) 267, 1254
TaaI ACNGT 3 cut(s) 790, 1094, 1292
TaiI ACGT 1 cut(s) 974
TaqI TCGA 3 cut(s) 104, 1060, 1387
TasI AATT 9 cut(s) 10, 151, 166, 626, 736, 892, 1132, 1211, 1471
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 4 cut(s) 179, 947, 1321, 1382
Tru1I TTAA 5 cut(s) 23, 381, 567, 603, 1458
Tru9I TTAA 5 cut(s) 23, 381, 567, 603, 1458
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 3 cut(s) 73, 242, 967
TseI GCWGC 4 cut(s) 38, 198, 536, 548
Tsp45I GTSAC 3 cut(s) 73, 242, 967
TspDTI ATGAA 3 cut(s) 179, 197, 895
TspGWI ACGGA 3 cut(s) 447, 687, 891
TspRI CASTG 1 cut(s) 793
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 1115, 1355
XapI RAATTY 3 cut(s) 151, 1132, 1211
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 4 cut(s) 344, 712, 841, 845
XspI CTAG 2 cut(s) 305, 660
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.