RchiOBHm_Chr1g0374571

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000663
Physical Location & Seq
Forward (+)
62359452 .. 62362844
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59837

Sequence Viewer

Length: 1365 bp
ATGAGTCTGTTTGGGAGATTAAGGACGGTGGTTTTACTGGCGGCGATGATGGTGGTGATGGTGGTGAAGGCTTCGCAGGACTATGGGGATGCATTGACGAAGAGCATATTGTTCTTTGAGGGCCAGAGGTCAGGGAAGCTACCCTCTCGTCAGAGAATGACTTGGAGGAAAGATTCTGCACTTAAGGATGGATTTCAGATTGGTGTTGATTTGGTAGGGGGTTACTACGATGCCGGTGACAATGTGAAGTTCAACTTTCCGATGGCTTTCTCGACAACCATGCTGGCATGGAGCGTGTTAGAGTTCGGGAAGGACATGGGTGCAGACCTACCCCGTGCACTTGATGCCATTCGATGGGGAACGGACTACTTCCTCAAAGCCACAAGCATTCCTGGCTTTGTGTTTGCCCAAGTTGGCGAACCTTACGGCGACCACAATTGTTGGGAGAGGCCTGAGGACATGGACACCCCAAGAAACCCCTATGCAGTTAGCAAACAGTTCCCAGGTTCTGAAGTCTCGGCAGAGATTGCAGCTGCCCTAGCAGCCTCTTCCATGGTTTTTAAGCATGTTGATTTGGAATACTCTGCAAGGCTTCTCAAAAGGGCTAGAATGGTGTTTGAGTTTGCAGAAACATACCGGGGCTCCTATAATGATGCTCTTGGACCATGGGTTTGTCCATTTTACTGTGATTTTAGTGGCTATGAGGACGAATTGATTTGGGGAGCAACATGGTTGTTTAAGGCAACTACGGACCCTACATACTCGAACTATGTAGTAGAAAACGTACACAAGCTGGAAAAATCTGTCATGAGAAATGTAAATGGTTTAGCATATTCTTCTGTAGGTGGAAGCTTTGCCGAATTTGGATGGGATTCAAAGAACGCAGGCATCAATGTACTGGCTTCCAAGCATGCAACAACTCTGTCATTTCTCTTGGTTGTTTATTCTCGTTATCTGAAGCAAGCCAATAGAGTAGTTCACTGTAACAATGTTGTTGCCAGTCCAGCTAGACTTGTTCAAGTTGCAAAAGGCCAGGTGGATTACATACTAGGAAGTAACCCATTGAACATGTCATATATGGTCGGGTATGGCAAAAAGTTTCCTCAGAGAATTCATCATCGGGGGTCATCGTTACCGTCGGTTGGTCAACACCCTAAACAGATTGGTTGCAAAGACGGAAGCGTTTACTTTAACAGCAGTAACCCAAATCCTAATATGCTAACTGGGGCTGTTGTTGGAGGACCAGATATAAAGGATTCATATGAAGATTCTCGAGTGGATTTTGTGCAGTCCGAGCCAACCACATACATCAATGCACCATTTGTAGGCGTCTTGGCTTACTTCAAATCGCATCCATGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

454

Amino Acids

50.41

Weight (kDa)

8.1

Isoelectric Point (pI)

28.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 306 3.9e-97 Glycosyl hydrolase family 9
Glyco_hydro_9 PF00759 303 - 445 1.5e-40 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 354
AciI CCGC 1 cut(s) 41
AcsI RAATTY 2 cut(s) 860, 1110
AcuI CTGAAG 2 cut(s) 531, 977
AcyI GRCGYC 1 cut(s) 1329
AfaI GTAC 2 cut(s) 786, 897
AfiI CCNNNNNNNGG 3 cut(s) 354, 1142, 1325
AflII CTTAAG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 5 cut(s) 253, 876, 1019, 1066, 1345
AjnI CCWGG 3 cut(s) 391, 502, 1032
AluBI AGCT 5 cut(s) 139, 533, 793, 852, 1007
AluI AGCT 5 cut(s) 139, 533, 793, 852, 1007
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 1 cut(s) 520
Alw44I GTGCAC 1 cut(s) 336
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 1272
AoxI GGCC 3 cut(s) 121, 449, 1030
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 3 cut(s) 530, 533, 542
ApoI RAATTY 2 cut(s) 860, 1110
ArsI GACNNNNNNTTYG 2 cut(s) 654, 686
AspS9I GGNCC 5 cut(s) 121, 662, 751, 1241, 1359
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 3 cut(s) 67, 76, 248
AvaI CYCGRG 1 cut(s) 1272
AvaII GGWCC 4 cut(s) 662, 751, 1241, 1359
AxyI CCTNAGG 1 cut(s) 453
BaeGI GKGCMC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 3 cut(s) 520, 542, 554
BccI CCATC 6 cut(s) 43, 52, 182, 256, 348, 861
BceAI ACGGC 1 cut(s) 442
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 3 cut(s) 393, 504, 1034
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 4 cut(s) 539, 606, 1008, 1049
BfmI CTRYAG 1 cut(s) 840
BfrI CTTAAG 1 cut(s) 182
BisI GCNGC 4 cut(s) 42, 531, 534, 543
BlsI GCNGC 4 cut(s) 43, 532, 535, 544
Bme1390I CCNGG 4 cut(s) 393, 504, 638, 1034
Bme18I GGWCC 4 cut(s) 662, 751, 1241, 1359
BmeT110I CYCGRG 1 cut(s) 1272
BmgT120I GGNCC 5 cut(s) 121, 662, 751, 1241, 1359
BmiI GGNNCC 2 cut(s) 643, 753
BmrFI CCNGG 4 cut(s) 393, 504, 638, 1034
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1360
BmuI ACTGGG 1 cut(s) 1233
BpuMI CCSGG 1 cut(s) 638
BsaHI GRCGYC 1 cut(s) 1329
BsaJI CCNNGG 5 cut(s) 502, 552, 637, 665, 1355
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 1142, 1325
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 4 cut(s) 42, 903, 999, 1228
Bse21I CCTNAGG 1 cut(s) 453
BseBI CCWGG 3 cut(s) 393, 504, 1034
BseDI CCNNGG 5 cut(s) 502, 552, 637, 665, 1355
BseGI GGATG 4 cut(s) 94, 193, 872, 1351
BseLI CCNNNNNNNGG 3 cut(s) 354, 1142, 1325
BseMII CTCAG 2 cut(s) 444, 1118
BseNI ACTGG 4 cut(s) 42, 903, 999, 1228
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 3 cut(s) 520, 542, 554
BsgI GTGCAG 3 cut(s) 162, 342, 1307
BshFI GGCC 3 cut(s) 123, 451, 1032
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 1272
BsiSI CCGG 2 cut(s) 234, 637
BslI CCNNNNNNNGG 3 cut(s) 354, 1142, 1325
BsmAI GTCTC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 3 cut(s) 123, 451, 1032
BsoBI CYCGRG 1 cut(s) 1272
Bsp1286I GDGCHC 2 cut(s) 340, 644
Bsp19I CCATGG 3 cut(s) 552, 665, 1355
BspACI CCGC 1 cut(s) 41
BspANI GGCC 3 cut(s) 123, 451, 1032
BspCNI CTCAG 2 cut(s) 445, 1117
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 2 cut(s) 643, 753
BspQI GCTCTTC 1 cut(s) 95
BspTI CTTAAG 1 cut(s) 182
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 4 cut(s) 42, 903, 999, 1228
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 502, 552, 637, 665, 1355
BssNI GRCGYC 1 cut(s) 1329
BssT1I CCWWGG 3 cut(s) 552, 665, 1355
Bst2UI CCWGG 3 cut(s) 393, 504, 1034
Bst4CI ACNGT 5 cut(s) 28, 498, 686, 983, 1137
Bst6I CTCTTC 2 cut(s) 95, 553
BstACI GRCGYC 1 cut(s) 1329
BstAFI CTTAAG 1 cut(s) 182
BstAPI GCANNNNNTGC 2 cut(s) 344, 527
BstC8I GCNNGC 4 cut(s) 285, 886, 912, 963
BstDEI CTNAG 2 cut(s) 453, 1104
BstDSI CCRYGG 3 cut(s) 552, 665, 1355
BstF5I GGATG 4 cut(s) 94, 193, 872, 1351
BstMAI GTCTC 1 cut(s) 520
BstMWI GCNNNNNNNGC 7 cut(s) 344, 393, 527, 539, 542, 1004, 1294
BstNI CCWGG 3 cut(s) 393, 504, 1034
BstNSI RCATGY 3 cut(s) 569, 914, 1072
BstSCI CCNGG 4 cut(s) 391, 502, 636, 1032
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 520, 542, 554
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 3 cut(s) 123, 451, 1032
BtgI CCRYGG 3 cut(s) 552, 665, 1355
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 4 cut(s) 94, 193, 872, 1351
BtsIMutI CAGTG 1 cut(s) 979
Cac8I GCNNGC 4 cut(s) 285, 886, 912, 963
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 807
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 5 cut(s) 121, 662, 751, 1241, 1359
CseI GACGC 1 cut(s) 1318
Csp6I GTAC 2 cut(s) 785, 896
CspCI CAANNNNNGTGG 2 cut(s) 422, 457
CviQI GTAC 2 cut(s) 785, 896
DdeI CTNAG 2 cut(s) 453, 1104
Eam1104I CTCTTC 2 cut(s) 95, 553
EarI CTCTTC 2 cut(s) 95, 553
Eco130I CCWWGG 3 cut(s) 552, 665, 1355
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 644
Eco47I GGWCC 4 cut(s) 662, 751, 1241, 1359
Eco57I CTGAAG 2 cut(s) 531, 977
Eco81I CCTNAGG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 1272
EcoRI GAATTC 1 cut(s) 1110
EcoRII CCWGG 3 cut(s) 391, 502, 1032
EcoT14I CCWWGG 3 cut(s) 552, 665, 1355
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 3 cut(s) 552, 665, 1355
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FauNDI CATATG 1 cut(s) 1261
Fnu4HI GCNGC 4 cut(s) 42, 531, 534, 543
FokI GGATG 4 cut(s) 101, 200, 879, 1338
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 4 cut(s) 42, 531, 534, 543
FspBI CTAG 4 cut(s) 539, 606, 1008, 1049
GluI GCNGC 4 cut(s) 42, 531, 534, 543
HaeIII GGCC 3 cut(s) 123, 451, 1032
HapII CCGG 2 cut(s) 234, 637
HgaI GACGC 1 cut(s) 1318
Hin1I GRCGYC 1 cut(s) 1329
HincII GTYRAC 1 cut(s) 1148
HindII GTYRAC 1 cut(s) 1148
HindIII AAGCTT 1 cut(s) 850
HinfI GANTC 5 cut(s) 4, 173, 872, 1256, 1268
HpaII CCGG 2 cut(s) 234, 637
HphI GGTGA 3 cut(s) 67, 76, 248
Hpy166II GTNNAC 5 cut(s) 338, 787, 979, 1148, 1186
Hpy188I TCNGA 7 cut(s) 153, 198, 261, 511, 957, 1107, 1294
Hpy188III TCNNGA 4 cut(s) 271, 307, 808, 1272
Hpy8I GTNNAC 5 cut(s) 338, 787, 979, 1148, 1186
Hpy99I CGWCG 1 cut(s) 1141
HpyAV CCTTC 2 cut(s) 61, 304
HpyCH4III ACNGT 5 cut(s) 28, 498, 686, 983, 1137
HpyCH4IV ACGT 1 cut(s) 783
HpyF10VI GCNNNNNNNGC 7 cut(s) 344, 393, 527, 539, 542, 1004, 1294
HpyF3I CTNAG 2 cut(s) 453, 1104
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 1329
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 3 cut(s) 291, 647, 722
Lsp1109I GCAGC 3 cut(s) 520, 542, 554
LweI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1360
MaeI CTAG 4 cut(s) 539, 606, 1008, 1049
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 6 cut(s) 221, 236, 983, 1055, 1131, 1199
MboII GAAGA 4 cut(s) 112, 540, 828, 1277
MfeI CAATTG 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 340, 644
MluCI AATT 4 cut(s) 436, 710, 860, 1110
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 1216
Mph1103I ATGCAT 1 cut(s) 94
MseI TTAA 5 cut(s) 20, 183, 561, 738, 1191
MspA1I CMGCKG 1 cut(s) 533
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 2 cut(s) 234, 637
MspR9I CCNGG 4 cut(s) 393, 504, 638, 1034
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 3 cut(s) 393, 504, 1034
MwoI GCNNNNNNNGC 7 cut(s) 344, 393, 527, 539, 542, 1004, 1294
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 3 cut(s) 552, 665, 1355
NdeI CATATG 1 cut(s) 1261
NlaIV GGNNCC 2 cut(s) 643, 753
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 3 cut(s) 569, 914, 1072
PaeI GCATGC 1 cut(s) 914
PaeR7I CTCGAG 1 cut(s) 1272
PagI TCATGA 1 cut(s) 807
PceI AGGCCT 1 cut(s) 451
PciI ACATGT 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 95
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 4 cut(s) 173, 872, 1256, 1268
PflMI CCANNNNNTGG 1 cut(s) 354
PkrI GCNGC 4 cut(s) 43, 532, 535, 544
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 1068
Psp6I CCWGG 3 cut(s) 391, 502, 1032
PspGI CCWGG 3 cut(s) 391, 502, 1032
PspN4I GGNNCC 2 cut(s) 643, 753
PspPI GGNCC 5 cut(s) 121, 662, 751, 1241, 1359
PstNI CAGNNNCTG 1 cut(s) 509
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 2 cut(s) 786, 897
RsaNI GTAC 2 cut(s) 785, 896
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 5 cut(s) 20, 183, 561, 738, 1191
SatI GCNGC 4 cut(s) 42, 531, 534, 543
Sau96I GGNCC 5 cut(s) 121, 662, 751, 1241, 1359
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 4 cut(s) 393, 504, 638, 1034
SduI GDGCHC 2 cut(s) 340, 644
SfaNI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1360
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 1272
SinI GGWCC 4 cut(s) 662, 751, 1241, 1359
SlaI CTCGAG 1 cut(s) 1272
SmlI CTYRAG 2 cut(s) 182, 1272
SmoI CTYRAG 2 cut(s) 182, 1272
SphI GCATGC 1 cut(s) 914
Sse9I AATT 4 cut(s) 436, 710, 860, 1110
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 1 cut(s) 41
SspMI CTAG 4 cut(s) 539, 606, 1008, 1049
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 4 cut(s) 391, 502, 636, 1032
StyI CCWWGG 3 cut(s) 552, 665, 1355
TaaI ACNGT 5 cut(s) 28, 498, 686, 983, 1137
TaiI ACGT 1 cut(s) 786
TaqI TCGA 4 cut(s) 272, 352, 764, 1273
TasI AATT 4 cut(s) 436, 710, 860, 1110
TatI WGTACW 1 cut(s) 895
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 4 cut(s) 173, 872, 1256, 1268
Tru1I TTAA 5 cut(s) 20, 183, 561, 738, 1191
Tru9I TTAA 5 cut(s) 20, 183, 561, 738, 1191
TscAI CASTG 1 cut(s) 986
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 3 cut(s) 530, 533, 542
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 3 cut(s) 1103, 1248, 1278
TspGWI ACGGA 3 cut(s) 377, 764, 1191
TspRI CASTG 1 cut(s) 986
Van91I CCANNNNNTGG 1 cut(s) 354
Vha464I CTTAAG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 4 cut(s) 662, 751, 1241, 1359
XapI RAATTY 2 cut(s) 860, 1110
XceI RCATGY 3 cut(s) 569, 914, 1072
XhoI CTCGAG 1 cut(s) 1272
XspI CTAG 4 cut(s) 539, 606, 1008, 1049
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.