Rw4G034660

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000663
Physical Location & Seq
Reverse (-)
61657483 .. 61660837
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034660.1

Sequence Viewer

Length: 1470 bp
ATGGCGCGAAATGGCCTTTGCTTCCCTGGAAATGTTCCCGCATTTCGCGCAGCACTAGTCCTCTCGCTGCTCCTCCTGCTTCTCCACCCAACCAGCGCCGGCCACGACTATCACGACGCCCTCCGCAAGAGCATCCTCTTCTTCGAAGGCCAGCGCTCCGGCAAGCTCCCACCCGATCAGCGCCTCAAATGGCGCCGCGACTCCGCATTGCACGACGGGTCCACCGCCGGCGTTGTTACAGACTTATCCGGCGGGTACTACGACGCCGGCGACAACGTCAAGTTCGGCTTTCCAATGGCGTTCACGACGACTTTGCTGGCGTGGAGCATCATAGACTTCGGGAGGATCATGGGGTCGGAGCAGAGGAACGCCGTCAAGTCCTTACGTTGGGGAACCGACTACCTCCTGAAGGCGACGGCGGTTCCCGGCGTTGTCTTCGTCCAGGTCGGCGACCCCTACTCCGACCACAACTGCTGGGAGAGGCCGGAAGACATGGACACTCGTCGCACGGTGTACAAAATCGACCGGAACAACCCGGGATCCGACGTGGCAGGCGAAACCGCCGCTGCGCTGGCCGCTGCCTCCATCGTGTTCAGGTCACGTGACCCCGCTTACTCGAGACTGCTTCTCAATCGAGCCGTTAGGGTTTTCGAGTTCGCTGACACCCATCGTGGTGCGTACAGCTCCAGCCTCAGAAACGCCGTGTGCCCTTTTTACTGCGACGTGAACGGCTTCCAGGATGAGTTACTGTGGGGAGCAGCGTGGTTAGAATACATAGTGAGAAACGAGGTGGTGTTGAGAGCTGGAGATACCATTAACGAGTTTGGTTGGGATAACAAGCATGCTGGGATTAATGTCCTCATTTCTAAGGAAGTGCTTATGGGAAAAGCAGATTATTTCGAATCTTTCAAGCAGAATGCAGATGGATTTATATGCTCCGTTTTGCCTGGACTTACCCATACCCAAGTCCAATATTCTCCAGGTGGTTTGATCTTCAAGCCTGGAGGGAGTAACATGCAGCATGTAACTTCGCTTTCGTTCCTACTTTTGACTTATTCGAACTATCTAAGCCATTCCAATAAGAACGTGCCATGTGGCATGTCCTCCGCCTCCCCTGCCTTCCTCAAACAATTGGCTAAACGCCAGGTGGACTACATTTTGGGGGACAATCCATTGAGGATGTCTTACATGGTTGGATATGGGCCGCGTTACCCACAGAGGATACACCACCGGGGCAGCTCACTTCCGTCGGTTCAGGCCCATCCTGCTCGTATCGGATGCAAAGCAGGTTCTCGCTATTATCTGAGTCCAAATCCGAACCCGAACCTGCTGGTTGGGGCGGTCGTGGGGGGACCCAATAGCTCGGATGCATTTCCAGACTCAAGGCCTTACTTTCAAGAATCTGAGCCCACGACGTACATCAATGCGCCGCTCGTGGGCCTACTTTCATATTTTGCAGCCCACTATTGA

Protein Analysis

489

Amino Acids

53.94

Weight (kDa)

8.67

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 37 - 482 5.1e-150 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1277, 1335
AccB1I GGYRCC 1 cut(s) 192
AccBSI CCGCTC 1 cut(s) 1432
AccII CGCG 4 cut(s) 7, 48, 198, 1207
AclWI GGATC 3 cut(s) 353, 534, 547
AcoI YGGCCR 2 cut(s) 100, 573
AcuI CTGAAG 1 cut(s) 428
AcvI CACGTG 1 cut(s) 602
AcyI GRCGYC 3 cut(s) 117, 193, 264
AfaI GTAC 4 cut(s) 257, 515, 680, 1418
AfeI AGCGCT 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 387, 409, 1436
AgsI TTSAA 3 cut(s) 910, 997, 1397
AhlI ACTAGT 1 cut(s) 55
AjiI CACGTC 2 cut(s) 547, 724
AjnI CCWGG 7 cut(s) 25, 441, 735, 946, 979, 1000, 1143
AluBI AGCT 5 cut(s) 166, 684, 803, 1239, 1362
AluI AGCT 5 cut(s) 166, 684, 803, 1239, 1362
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 3 cut(s) 353, 534, 547
Ama87I CYCGRG 2 cut(s) 535, 616
Aor51HI AGCGCT 1 cut(s) 155
AoxI GGCC 9 cut(s) 13, 100, 148, 482, 573, 1202, 1257, 1385, 1438
ApeKI GCWGC 8 cut(s) 50, 67, 566, 578, 758, 1018, 1236, 1457
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 8 cut(s) 7, 50, 98, 156, 183, 195, 571, 1429
AspS9I GGNCC 5 cut(s) 219, 1202, 1258, 1352, 1438
AsuC2I CCSGG 4 cut(s) 426, 536, 537, 1232
AsuII TTCGAA 3 cut(s) 144, 900, 1058
AvaI CYCGRG 2 cut(s) 535, 616
AvaII GGWCC 2 cut(s) 219, 1352
BaeGI GKGCMC 1 cut(s) 710
BamHI GGATCC 1 cut(s) 539
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 1410
BauI CACGAG 1 cut(s) 1433
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 2 cut(s) 427, 495
BbvI GCAGC 7 cut(s) 54, 62, 553, 565, 770, 1030, 1248
BccI CCATC 4 cut(s) 593, 675, 917, 1269
BceAI ACGGC 5 cut(s) 356, 432, 623, 686, 745
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 7 cut(s) 27, 443, 737, 948, 981, 1002, 1145
BciVI GTATCC 1 cut(s) 1215
BcnI CCSGG 4 cut(s) 426, 536, 537, 1232
BcoDI GTCTC 1 cut(s) 613
BcuI ACTAGT 1 cut(s) 55
BfaI CTAG 1 cut(s) 56
BfoI RGCGCY 4 cut(s) 99, 157, 184, 196
BfuAI ACCTGC 2 cut(s) 1277, 1335
BfuI GTATCC 1 cut(s) 1215
Bme18I GGWCC 2 cut(s) 219, 1352
BmeT110I CYCGRG 2 cut(s) 535, 616
BmgBI CACGTC 2 cut(s) 547, 724
BmgT120I GGNCC 5 cut(s) 219, 1202, 1258, 1352, 1438
BmiI GGNNCC 7 cut(s) 194, 220, 394, 423, 541, 1353, 1354
BmsI GCATC 4 cut(s) 141, 336, 1268, 1357
BoxI GACNNNNGTC 1 cut(s) 501
BpiI GAAGAC 2 cut(s) 427, 495
BpmI CTGGAG 4 cut(s) 670, 825, 963, 1023
Bpu14I TTCGAA 3 cut(s) 144, 900, 1058
BpuEI CTTGAG 1 cut(s) 1366
BpuMI CCSGG 4 cut(s) 426, 536, 537, 1232
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 3 cut(s) 117, 193, 264
BsaJI CCNNGG 3 cut(s) 25, 535, 1231
BsaWI WCCGGW 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 409, 1436
Bse118I RCCGGY 3 cut(s) 98, 227, 266
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 7 cut(s) 27, 443, 737, 948, 981, 1002, 1145
BseDI CCNNGG 3 cut(s) 25, 535, 1231
BseGI GGATG 6 cut(s) 132, 745, 1185, 1261, 1283, 1372
BseLI CCNNNNNNNGG 3 cut(s) 387, 409, 1436
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 3 cut(s) 706, 1295, 1395
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 710
BseXI GCAGC 7 cut(s) 54, 62, 553, 565, 770, 1030, 1248
BseYI CCCAGC 2 cut(s) 474, 845
Bsh1236I CGCG 4 cut(s) 7, 48, 198, 1207
Bsh1285I CGRYCG 2 cut(s) 526, 1344
BshFI GGCC 9 cut(s) 15, 102, 150, 484, 575, 1204, 1259, 1387, 1440
BshNI GGYRCC 1 cut(s) 192
BsiEI CGRYCG 2 cut(s) 526, 1344
BsiHKCI CYCGRG 2 cut(s) 535, 616
BslFI GGGAC 2 cut(s) 1178, 1365
BslI CCNNNNNNNGG 3 cut(s) 387, 409, 1436
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 2 cut(s) 1178, 1365
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 9 cut(s) 15, 102, 150, 484, 575, 1204, 1259, 1387, 1440
BsoBI CYCGRG 2 cut(s) 535, 616
Bsp119I TTCGAA 3 cut(s) 144, 900, 1058
Bsp1286I GDGCHC 2 cut(s) 710, 1410
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 4 cut(s) 175, 345, 539, 990
BspANI GGCC 9 cut(s) 15, 102, 150, 484, 575, 1204, 1259, 1387, 1440
BspCNI CTCAG 3 cut(s) 705, 1296, 1396
BspFNI CGCG 4 cut(s) 7, 48, 198, 1207
BspLI GGNNCC 7 cut(s) 194, 220, 394, 423, 541, 1353, 1354
BspMI ACCTGC 2 cut(s) 1277, 1335
BspPI GGATC 3 cut(s) 353, 534, 547
BspT104I TTCGAA 3 cut(s) 144, 900, 1058
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 1432
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 3 cut(s) 98, 227, 266
BsrGI TGTACA 1 cut(s) 513
BssAI RCCGGY 3 cut(s) 98, 227, 266
BssECI CCNNGG 3 cut(s) 25, 535, 1231
BssMI GATC 4 cut(s) 175, 345, 539, 990
BssNI GRCGYC 3 cut(s) 117, 193, 264
BssSI CACGAG 1 cut(s) 1433
Bst2BI CACGAG 1 cut(s) 1433
Bst2UI CCWGG 7 cut(s) 27, 443, 737, 948, 981, 1002, 1145
Bst4CI ACNGT 2 cut(s) 511, 750
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 3 cut(s) 117, 193, 264
BstAUI TGTACA 1 cut(s) 513
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 3 cut(s) 144, 900, 1058
BstC8I GCNNGC 9 cut(s) 100, 152, 164, 229, 268, 318, 553, 573, 843
BstDEI CTNAG 5 cut(s) 692, 867, 1067, 1304, 1404
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 6 cut(s) 132, 745, 1185, 1261, 1283, 1372
BstFNI CGCG 4 cut(s) 7, 48, 198, 1207
BstH2I RGCGCY 4 cut(s) 99, 157, 184, 196
BstHHI GCGC 8 cut(s) 7, 50, 98, 156, 183, 195, 571, 1429
BstKTI GATC 4 cut(s) 178, 348, 542, 993
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 4 cut(s) 175, 345, 539, 990
BstMCI CGRYCG 2 cut(s) 526, 1344
BstMWI GCNNNNNNNGC 6 cut(s) 47, 76, 572, 575, 1115, 1265
BstNI CCWGG 7 cut(s) 27, 443, 737, 948, 981, 1002, 1145
BstNSI RCATGY 4 cut(s) 845, 1018, 1025, 1102
BstPAI GACNNNNGTC 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 710
BstUI CGCG 4 cut(s) 7, 48, 198, 1207
BstV1I GCAGC 7 cut(s) 54, 62, 553, 565, 770, 1030, 1248
BstV2I GAAGAC 2 cut(s) 427, 495
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
BsuI GTATCC 1 cut(s) 1215
BsuRI GGCC 9 cut(s) 15, 102, 150, 484, 575, 1204, 1259, 1387, 1440
BtrI CACGTC 2 cut(s) 547, 724
BtsCI GGATG 6 cut(s) 132, 745, 1185, 1261, 1283, 1372
BveI ACCTGC 2 cut(s) 1277, 1335
Cac8I GCNNGC 9 cut(s) 100, 152, 164, 229, 268, 318, 553, 573, 843
CfoI GCGC 8 cut(s) 7, 50, 98, 156, 183, 195, 571, 1429
Cfr10I RCCGGY 3 cut(s) 98, 227, 266
Cfr13I GGNCC 5 cut(s) 219, 1202, 1258, 1352, 1438
Cfr9I CCCGGG 1 cut(s) 535
CseI GACGC 2 cut(s) 125, 272
Csp6I GTAC 4 cut(s) 256, 514, 679, 1417
CviAII CATG 8 cut(s) 349, 493, 842, 1015, 1022, 1092, 1099, 1189
CviQI GTAC 4 cut(s) 256, 514, 679, 1417
DdeI CTNAG 5 cut(s) 692, 867, 1067, 1304, 1404
DinI GGCGCC 1 cut(s) 194
DpnI GATC 4 cut(s) 177, 347, 541, 992
DpnII GATC 4 cut(s) 175, 345, 539, 990
EaeI YGGCCR 2 cut(s) 100, 573
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 1096
Eco147I AGGCCT 1 cut(s) 1387
Eco24I GRGCYC 1 cut(s) 1410
Eco47I GGWCC 2 cut(s) 219, 1352
Eco47III AGCGCT 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 428
Eco72I CACGTG 1 cut(s) 602
Eco88I CYCGRG 2 cut(s) 535, 616
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 1352
EcoRII CCWGG 7 cut(s) 25, 441, 735, 946, 979, 1000, 1143
EcoT22I ATGCAT 1 cut(s) 1372
EcoT38I GRGCYC 1 cut(s) 1410
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
FaeI CATG 8 cut(s) 352, 496, 845, 1018, 1025, 1095, 1102, 1192
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 1178, 1365
FatI CATG 8 cut(s) 348, 492, 841, 1014, 1021, 1091, 1098, 1188
FauI CCCGC 3 cut(s) 46, 245, 616
FokI GGATG 6 cut(s) 119, 752, 1192, 1248, 1290, 1379
FriOI GRGCYC 1 cut(s) 1410
FspBI CTAG 1 cut(s) 56
GlaI GCGC 8 cut(s) 6, 49, 97, 155, 182, 194, 570, 1428
GsaI CCCAGC 2 cut(s) 478, 849
GsuI CTGGAG 4 cut(s) 670, 825, 963, 1023
HaeII RGCGCY 4 cut(s) 99, 157, 184, 196
HaeIII GGCC 9 cut(s) 15, 102, 150, 484, 575, 1204, 1259, 1387, 1440
HgaI GACGC 2 cut(s) 125, 272
HhaI GCGC 8 cut(s) 7, 50, 98, 156, 183, 195, 571, 1429
Hin1I GRCGYC 3 cut(s) 117, 193, 264
Hin1II CATG 8 cut(s) 352, 496, 845, 1018, 1025, 1095, 1102, 1192
Hin6I GCGC 8 cut(s) 5, 48, 96, 154, 181, 193, 569, 1427
HinP1I GCGC 8 cut(s) 5, 48, 96, 154, 181, 193, 569, 1427
HinfI GANTC 5 cut(s) 200, 902, 1306, 1379, 1400
Hpy166II GTNNAC 5 cut(s) 222, 303, 514, 727, 1150
Hpy188I TCNGA 9 cut(s) 358, 463, 544, 695, 1277, 1305, 1317, 1366, 1405
Hpy188III TCNNGA 7 cut(s) 113, 304, 340, 406, 618, 1376, 1397
Hpy8I GTNNAC 5 cut(s) 222, 303, 514, 727, 1150
HpyAV CCTTC 3 cut(s) 140, 403, 1129
HpyCH4III ACNGT 2 cut(s) 511, 750
HpyCH4IV ACGT 7 cut(s) 276, 385, 546, 601, 723, 1086, 1415
HpyCH4V TGCA 6 cut(s) 211, 920, 1018, 1281, 1370, 1457
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 76, 572, 575, 1115, 1265
HpyF3I CTNAG 5 cut(s) 692, 867, 1067, 1304, 1404
HpySE526I ACGT 7 cut(s) 276, 385, 546, 601, 723, 1086, 1415
Hsp92I GRCGYC 3 cut(s) 117, 193, 264
Hsp92II CATG 8 cut(s) 352, 496, 845, 1018, 1025, 1095, 1102, 1192
HspAI GCGC 8 cut(s) 5, 48, 96, 154, 181, 193, 569, 1427
KasI GGCGCC 1 cut(s) 192
KflI GGGWCCC 1 cut(s) 1352
KroI GCCGGC 3 cut(s) 98, 227, 266
KroNI GCCGGC 3 cut(s) 100, 229, 268
Kzo9I GATC 4 cut(s) 175, 345, 539, 990
LmnI GCTCC 8 cut(s) 75, 161, 171, 324, 358, 689, 755, 941
Lsp1109I GCAGC 7 cut(s) 54, 62, 553, 565, 770, 1030, 1248
LweI GCATC 4 cut(s) 141, 336, 1268, 1357
MaeI CTAG 1 cut(s) 56
MaeII ACGT 7 cut(s) 276, 385, 546, 601, 723, 1086, 1415
MaeIII GTNAC 7 cut(s) 235, 597, 602, 744, 1010, 1024, 1208
MalI GATC 4 cut(s) 177, 347, 541, 992
MbiI CCGCTC 1 cut(s) 1432
MboI GATC 4 cut(s) 175, 345, 539, 990
MboII GAAGA 5 cut(s) 130, 133, 427, 500, 985
MfeI CAATTG 1 cut(s) 1130
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 2 cut(s) 710, 1410
MluCI AATT 1 cut(s) 1130
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 3 cut(s) 194, 1315, 1373
MmeI TCCRAC 4 cut(s) 336, 486, 567, 1174
Mph1103I ATGCAT 1 cut(s) 1372
MreI CGCCGGCG 2 cut(s) 227, 266
MroNI GCCGGC 3 cut(s) 98, 227, 266
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 2 cut(s) 816, 852
MslI CAYNNNNRTG 1 cut(s) 672
MspA1I CMGCKG 2 cut(s) 566, 578
MunI CAATTG 1 cut(s) 1130
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 7 cut(s) 27, 443, 737, 948, 981, 1002, 1145
MvnI CGCG 4 cut(s) 7, 48, 198, 1207
MwoI GCNNNNNNNGC 6 cut(s) 47, 76, 572, 575, 1115, 1265
NaeI GCCGGC 3 cut(s) 100, 229, 268
NarI GGCGCC 1 cut(s) 193
NciI CCSGG 4 cut(s) 426, 536, 537, 1232
NdeII GATC 4 cut(s) 175, 345, 539, 990
NgoMIV GCCGGC 3 cut(s) 98, 227, 266
NlaIII CATG 8 cut(s) 352, 496, 845, 1018, 1025, 1095, 1102, 1192
NlaIV GGNNCC 7 cut(s) 194, 220, 394, 423, 541, 1353, 1354
NmuCI GTSAC 2 cut(s) 597, 602
NsiI ATGCAT 1 cut(s) 1372
NspI RCATGY 4 cut(s) 845, 1018, 1025, 1102
NspV TTCGAA 3 cut(s) 144, 900, 1058
PaeI GCATGC 1 cut(s) 845
PaeR7I CTCGAG 1 cut(s) 616
PceI AGGCCT 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 267, 552
PctI GAATGC 1 cut(s) 922
PdiI GCCGGC 3 cut(s) 100, 229, 268
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 2 cut(s) 902, 1400
PflFI GACNNNGTC 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 735
PleI GAGTC 3 cut(s) 194, 1314, 1373
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 3 cut(s) 194, 1314, 1373
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 1352
PshAI GACNNNNGTC 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 1352
Psp6I CCWGG 7 cut(s) 25, 441, 735, 946, 979, 1000, 1143
PspCI CACGTG 1 cut(s) 602
PspFI CCCAGC 2 cut(s) 474, 845
PspGI CCWGG 7 cut(s) 25, 441, 735, 946, 979, 1000, 1143
PspN4I GGNNCC 7 cut(s) 194, 220, 394, 423, 541, 1353, 1354
PspPI GGNCC 5 cut(s) 219, 1202, 1258, 1352, 1438
PspPPI RGGWCCY 1 cut(s) 1352
PsuI RGATCY 1 cut(s) 539
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 4 cut(s) 257, 515, 680, 1418
RsaNI GTAC 4 cut(s) 256, 514, 679, 1417
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 2 cut(s) 816, 852
Sau3AI GATC 4 cut(s) 175, 345, 539, 990
Sau96I GGNCC 5 cut(s) 219, 1202, 1258, 1352, 1438
SchI GAGTC 3 cut(s) 194, 1315, 1373
SduI GDGCHC 2 cut(s) 710, 1410
SfaNI GCATC 4 cut(s) 141, 336, 1268, 1357
SfoI GGCGCC 1 cut(s) 194
Sfr274I CTCGAG 1 cut(s) 616
SfuI TTCGAA 3 cut(s) 144, 900, 1058
SgrAI CRCCGGYG 2 cut(s) 227, 266
SinI GGWCC 2 cut(s) 219, 1352
SlaI CTCGAG 1 cut(s) 616
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 2 cut(s) 616, 1381
SmoI CTYRAG 2 cut(s) 616, 1381
SpeI ACTAGT 1 cut(s) 55
SphI GCATGC 1 cut(s) 845
Sse9I AATT 1 cut(s) 1130
SseBI AGGCCT 1 cut(s) 1387
SspDI GGCGCC 1 cut(s) 192
SspI AATATT 1 cut(s) 974
SspMI CTAG 1 cut(s) 56
StuI AGGCCT 1 cut(s) 1387
TaaI ACNGT 2 cut(s) 511, 750
TaiI ACGT 7 cut(s) 279, 388, 549, 604, 726, 1089, 1418
TaqI TCGA 7 cut(s) 144, 522, 617, 634, 651, 900, 1058
TasI AATT 1 cut(s) 1130
TatI WGTACW 1 cut(s) 513
TauI GCSGC 5 cut(s) 198, 566, 578, 1207, 1432
TfiI GAWTC 2 cut(s) 902, 1400
Tru1I TTAA 2 cut(s) 816, 852
Tru9I TTAA 2 cut(s) 816, 852
TseFI GTSAC 2 cut(s) 597, 602
TseI GCWGC 8 cut(s) 50, 67, 566, 578, 758, 1018, 1236, 1457
Tsp45I GTSAC 2 cut(s) 597, 602
TspDTI ATGAA 1 cut(s) 1437
TspGWI ACGGA 2 cut(s) 928, 1236
TspMI CCCGGG 1 cut(s) 535
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 219, 1352
VspI ATTAAT 1 cut(s) 852
XagI CCTNNNNNAGG 1 cut(s) 407
XceI RCATGY 4 cut(s) 845, 1018, 1025, 1102
XhoI CTCGAG 1 cut(s) 616
XmaI CCCGGG 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 1 cut(s) 56
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.