RLG00000026724

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000663
Physical Location & Seq
Reverse (-)
5322481 .. 5334250
11770 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026724

Sequence Viewer

Length: 1362 bp
ATGGCAACACGAGTAAGCTCTGCGGTTCAACATAAGTACGCGGATGCTTTGTCAAAGAGCATTTTGTTTTTTGAAGGGCAGAGATCGGGGAGGTTGCCGCATACACAACGAATGACATGGAGGAAAGATTCTGCTCTACGTAATGGCCTAGATGTTAAAAGGGATCTGACCGGGGGTTATTATGATGCGGGTGACAATGTGAAATTTAACTTTCCAATGACTTTCACCACAATAATGTTGTCATGGAGTGTTATAAAATTCGGAAAATCAATGGGCTCAGAACTTCCACATGCCTTGGAACTTGGAAGCCTCGGTGACCCCAATGGTGACCACACTTGTTGGGAAAGGCCTGAAGACAAGGACACTCCTCGAACATCTTACATAGTAACCACACAAAAGCCAGGTTTTGAAGTTTCAGCCGAGATTGCAGCTTCTTCCATGGTGTTTAGTGGTTCTGACAAAGGATATTCTACCTTGCTTCTTACCCGAGCAAGACAGGTGTTTGAATTTGCTGATAAATATAGGGGTTCTTACAATGACAGTATTGGGAGTGGAGCATGCCCATTTTACTGTGACTTCAATGGCTACAAGGATGAATTGATTTGGGGAGCAGCATGGTTATACAGCGCAATAAGGAGTCCTAATTATTGGAACTATGTAAAACAGAACATAGATGCCATGAATTTGGAGTCCAATATAGTCCTAAGAAATGTGGAAGGCCAAGCAGTTGCATACAATGGCGGAGATGTTACCGAATTTGGATGGGATGCAAAGCCTGCTGGCATTAACATCCTTGTTTCTCAATGGGTGATGACTAACCCCGTCGAGGCCAATCCCTTCATTCCCAAAGCGGATAGGCTTGTTTGCTCCATTGTGCCCGAATCACCTGCACCCAATTCAATCACAACATACAACCGCTTTACCTTTTCTTCTAGTAGTTATGCTGGCTATATGAAGGCTGCAAGAAAAGTTGTGAATTGTGGGAACAATGTTGTTGTCACTCCAGCTAGGCTGGTGGAACTCACAAAAGGACAGGTTGATTATATACTGGGAAGCAATCCTAATAGCATGTCATACATGGTGGGATATGGTACAAGATTTCCCCTGAAGATACATTACCGCGGATCGGTGATACCTTCGATTGATCAACACCCCAAACACATGCAGTGTCATGAAGGAGACATGTATTTCAAAGCTCAGGCTCCGAATCCAAACTTGCAGATAGGTGGAATCGTTGGAGGACCAGCTCTGGATGATACATTCGAAGATTCTCGATATAATGTTCCACAATCGGAGCCAACCACATACATTGATGCACCTTTTGTTGGTGTTTTGGCTTACTTCAAATCACCATCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

454

Amino Acids

50.22

Weight (kDa)

6.72

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 13 - 445 4.6e-106 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AarI CACCTGC 1 cut(s) 895
Acc36I ACCTGC 1 cut(s) 895
AccII CGCG 2 cut(s) 41, 1122
AciI CCGC 9 cut(s) 23, 41, 98, 188, 741, 851, 916, 1120, 1122
AclWI GGATC 2 cut(s) 171, 1132
AcsI RAATTY 5 cut(s) 203, 257, 506, 682, 755
AcuI CTGAAG 2 cut(s) 372, 1127
AfaI GTAC 2 cut(s) 38, 1093
AfiI CCNNNNNNNGG 3 cut(s) 826, 1126, 1325
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 8 cut(s) 29, 74, 410, 506, 580, 900, 1192, 1345
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 5 cut(s) 18, 431, 1007, 1196, 1247
AluI AGCT 5 cut(s) 18, 431, 1007, 1196, 1247
Alw26I GTCTC 1 cut(s) 1173
AlwI GGATC 2 cut(s) 171, 1132
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 4 cut(s) 145, 347, 718, 828
ApeKI GCWGC 3 cut(s) 428, 611, 959
ApoI RAATTY 5 cut(s) 203, 257, 506, 682, 755
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 1 cut(s) 1241
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 8 cut(s) 203, 217, 326, 338, 820, 876, 1141, 1341
AsuII TTCGAA 1 cut(s) 1263
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 1 cut(s) 1241
BaeGI GKGCMC 1 cut(s) 879
BanII GRGCYC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 1100, 1132
BauI CACGAG 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 440, 623, 946
BccI CCATC 2 cut(s) 756, 1360
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 1 cut(s) 402
BclI TGATCA 1 cut(s) 1144
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 1173
BfaI CTAG 3 cut(s) 149, 933, 1008
BfuAI ACCTGC 1 cut(s) 895
BisI GCNGC 4 cut(s) 98, 429, 612, 960
BlsI GCNGC 4 cut(s) 99, 430, 613, 961
Bme1390I CCNGG 2 cut(s) 172, 402
Bme18I GGWCC 1 cut(s) 1241
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 1241
BmiI GGNNCC 2 cut(s) 1203, 1296
BmrFI CCNGG 2 cut(s) 172, 402
BmrI ACTGGG 1 cut(s) 1058
BmsI GCATC 5 cut(s) 34, 175, 664, 757, 1303
BmuI ACTGGG 1 cut(s) 1058
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 1197
Bpu14I TTCGAA 1 cut(s) 1263
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 140
BsaJI CCNNGG 5 cut(s) 171, 294, 310, 438, 1120
Bsc4I CCNNNNNNNGG 3 cut(s) 826, 1126, 1325
Bse1I ACTGG 1 cut(s) 1053
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 5 cut(s) 171, 294, 310, 438, 1120
BseGI GGATG 6 cut(s) 49, 598, 767, 772, 789, 1258
BseLI CCNNNNNNNGG 3 cut(s) 826, 1126, 1325
BseMII CTCAG 2 cut(s) 291, 1211
BseNI ACTGG 1 cut(s) 1053
BseRI GAGGAG 1 cut(s) 357
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 3 cut(s) 440, 623, 946
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 2 cut(s) 41, 1122
BshFI GGCC 4 cut(s) 147, 349, 720, 830
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 3 cut(s) 826, 1126, 1325
BsmAI GTCTC 1 cut(s) 1173
BsnI GGCC 4 cut(s) 147, 349, 720, 830
BsoBI CYCGRG 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 1263
Bsp1286I GDGCHC 2 cut(s) 278, 879
Bsp143I GATC 4 cut(s) 83, 163, 1124, 1144
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 9 cut(s) 23, 41, 98, 188, 741, 851, 916, 1120, 1122
BspANI GGCC 4 cut(s) 147, 349, 720, 830
BspCNI CTCAG 2 cut(s) 290, 1210
BspFNI CGCG 2 cut(s) 41, 1122
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 2 cut(s) 1203, 1296
BspMI ACCTGC 1 cut(s) 895
BspPI GGATC 2 cut(s) 171, 1132
BspT104I TTCGAA 1 cut(s) 1263
BsrI ACTGG 1 cut(s) 1053
BssECI CCNNGG 5 cut(s) 171, 294, 310, 438, 1120
BssMI GATC 4 cut(s) 83, 163, 1124, 1144
BssSI CACGAG 1 cut(s) 9
BssT1I CCWWGG 2 cut(s) 294, 438
Bst2BI CACGAG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 542, 572
BstAPI GCANNNNNTGC 1 cut(s) 776
BstBAI YACGTR 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 1263
BstC8I GCNNGC 4 cut(s) 559, 777, 781, 946
BstDEI CTNAG 3 cut(s) 277, 704, 1197
BstDSI CCRYGG 2 cut(s) 438, 1120
BstEII GGTNACC 2 cut(s) 314, 326
BstF5I GGATG 6 cut(s) 49, 598, 767, 772, 789, 1258
BstFNI CGCG 2 cut(s) 41, 1122
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 4 cut(s) 86, 166, 1127, 1147
BstMAI GTCTC 1 cut(s) 1173
BstMBI GATC 4 cut(s) 83, 163, 1124, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 425, 776
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 5 cut(s) 293, 561, 1072, 1165, 1186
BstPI GGTNACC 2 cut(s) 314, 326
BstSCI CCNGG 2 cut(s) 170, 400
BstSLI GKGCMC 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 140
BstUI CGCG 2 cut(s) 41, 1122
BstV1I GCAGC 3 cut(s) 440, 623, 946
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 1 cut(s) 163
BsuRI GGCC 4 cut(s) 147, 349, 720, 830
BtgI CCRYGG 2 cut(s) 438, 1120
BtsCI GGATG 6 cut(s) 49, 598, 767, 772, 789, 1258
BtsI GCAGTG 1 cut(s) 1172
BtsIMutI CAGTG 1 cut(s) 1172
BveI ACCTGC 1 cut(s) 895
Cac8I GCNNGC 4 cut(s) 559, 777, 781, 946
CciI TCATGA 1 cut(s) 1171
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 1241
Cfr42I CCGCGG 1 cut(s) 1123
Csp6I GTAC 2 cut(s) 37, 1092
CspCI CAANNNNNGTGG 6 cut(s) 276, 311, 320, 355, 1291, 1326
CviQI GTAC 2 cut(s) 37, 1092
DdeI CTNAG 3 cut(s) 277, 704, 1197
DpnI GATC 4 cut(s) 85, 165, 1126, 1146
DpnII GATC 4 cut(s) 83, 163, 1124, 1144
EciI GGCGGA 1 cut(s) 756
Eco105I TACGTA 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 294, 438
Eco147I AGGCCT 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 1241
Eco57I CTGAAG 2 cut(s) 372, 1127
Eco88I CYCGRG 1 cut(s) 486
Eco91I GGTNACC 2 cut(s) 314, 326
EcoO65I GGTNACC 2 cut(s) 314, 326
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 294, 438
EcoT38I GRGCYC 1 cut(s) 278
ErhI CCWWGG 2 cut(s) 294, 438
FauI CCCGC 1 cut(s) 181
FbaI TGATCA 1 cut(s) 1144
Fnu4HI GCNGC 4 cut(s) 98, 429, 612, 960
FokI GGATG 6 cut(s) 56, 605, 774, 776, 779, 1265
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 4 cut(s) 98, 429, 612, 960
FspBI CTAG 3 cut(s) 149, 933, 1008
GlaI GCGC 1 cut(s) 628
GluI GCNGC 4 cut(s) 98, 429, 612, 960
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 4 cut(s) 147, 349, 720, 830
HapII CCGG 1 cut(s) 171
HhaI GCGC 1 cut(s) 629
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HinfI GANTC 7 cut(s) 128, 637, 689, 881, 1207, 1230, 1268
HpaII CCGG 1 cut(s) 171
HphI GGTGA 8 cut(s) 203, 217, 326, 338, 820, 876, 1141, 1341
Hpy188I TCNGA 6 cut(s) 168, 263, 280, 457, 1206, 1294
Hpy188III TCNNGA 3 cut(s) 1172, 1250, 1272
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 6 cut(s) 68, 710, 847, 949, 1146, 1169
HpyCH4III ACNGT 2 cut(s) 542, 572
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 8 cut(s) 428, 731, 770, 890, 962, 1165, 1219, 1316
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 776
HpyF3I CTNAG 3 cut(s) 277, 704, 1197
HpySE526I ACGT 1 cut(s) 139
HspAI GCGC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 1144
KspI CCGCGG 1 cut(s) 1123
Kzo9I GATC 4 cut(s) 83, 163, 1124, 1144
LmnI GCTCC 5 cut(s) 554, 608, 872, 1207, 1294
Lsp1109I GCAGC 3 cut(s) 440, 623, 946
LweI GCATC 5 cut(s) 34, 175, 664, 757, 1303
MaeI CTAG 3 cut(s) 149, 933, 1008
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 7 cut(s) 191, 314, 326, 385, 572, 748, 997
MalI GATC 4 cut(s) 85, 165, 1126, 1146
MboI GATC 4 cut(s) 83, 163, 1124, 1144
MboII GAAGA 5 cut(s) 365, 426, 921, 1120, 1277
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 278, 879
MluCI AATT 9 cut(s) 203, 257, 506, 596, 643, 682, 755, 895, 976
MlyI GAGTC 2 cut(s) 646, 698
MmeI TCCRAC 1 cut(s) 1216
MnlI CCTC 6 cut(s) 84, 114, 320, 378, 820, 1232
MseI TTAA 3 cut(s) 156, 207, 786
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 1122
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 2 cut(s) 172, 402
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 41, 1122
MwoI GCNNNNNNNGC 2 cut(s) 425, 776
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 1 cut(s) 438
NdeII GATC 4 cut(s) 83, 163, 1124, 1144
NlaIV GGNNCC 2 cut(s) 1203, 1296
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 5 cut(s) 191, 314, 326, 572, 997
NspI RCATGY 5 cut(s) 293, 561, 1072, 1165, 1186
NspV TTCGAA 1 cut(s) 1263
PaeI GCATGC 1 cut(s) 561
PagI TCATGA 1 cut(s) 1171
PaqCI CACCTGC 1 cut(s) 895
PceI AGGCCT 1 cut(s) 349
PciI ACATGT 1 cut(s) 1182
PfeI GAWTC 5 cut(s) 128, 881, 1207, 1230, 1268
PkrI GCNGC 4 cut(s) 99, 430, 613, 961
PleI GAGTC 2 cut(s) 645, 697
PpsI GAGTC 2 cut(s) 645, 697
Ppu21I YACGTR 1 cut(s) 140
PscI ACATGT 1 cut(s) 1182
PsiI TTATAA 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 400
PspEI GGTNACC 2 cut(s) 314, 326
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 1203, 1296
PspPI GGNCC 1 cut(s) 1241
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 2 cut(s) 38, 1093
RsaNI GTAC 2 cut(s) 37, 1092
RseI CAYNNNNRTG 1 cut(s) 233
SacII CCGCGG 1 cut(s) 1123
SaqAI TTAA 3 cut(s) 156, 207, 786
SatI GCNGC 4 cut(s) 98, 429, 612, 960
Sau3AI GATC 4 cut(s) 83, 163, 1124, 1144
Sau96I GGNCC 1 cut(s) 1241
SchI GAGTC 2 cut(s) 646, 698
ScrFI CCNGG 2 cut(s) 172, 402
SduI GDGCHC 2 cut(s) 278, 879
SfaNI GCATC 5 cut(s) 34, 175, 664, 757, 1303
Sfr303I CCGCGG 1 cut(s) 1123
SfuI TTCGAA 1 cut(s) 1263
SgrBI CCGCGG 1 cut(s) 1123
SinI GGWCC 1 cut(s) 1241
SmiMI CAYNNNNRTG 1 cut(s) 233
SnaBI TACGTA 1 cut(s) 140
SphI GCATGC 1 cut(s) 561
Sse9I AATT 9 cut(s) 203, 257, 506, 596, 643, 682, 755, 895, 976
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 9 cut(s) 23, 41, 98, 188, 741, 851, 916, 1120, 1122
SspMI CTAG 3 cut(s) 149, 933, 1008
StuI AGGCCT 1 cut(s) 349
StyD4I CCNGG 2 cut(s) 170, 400
StyI CCWWGG 2 cut(s) 294, 438
TaaI ACNGT 2 cut(s) 542, 572
TaiI ACGT 1 cut(s) 142
TaqI TCGA 5 cut(s) 370, 825, 1139, 1263, 1273
TasI AATT 9 cut(s) 203, 257, 506, 596, 643, 682, 755, 895, 976
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 5 cut(s) 128, 881, 1207, 1230, 1268
Tru1I TTAA 3 cut(s) 156, 207, 786
Tru9I TTAA 3 cut(s) 156, 207, 786
TscAI CASTG 1 cut(s) 1172
TseFI GTSAC 5 cut(s) 191, 314, 326, 572, 997
TseI GCWGC 3 cut(s) 428, 611, 959
Tsp45I GTSAC 5 cut(s) 191, 314, 326, 572, 997
TspDTI ATGAA 5 cut(s) 609, 695, 829, 968, 1188
TspRI CASTG 1 cut(s) 1172
VpaK11BI GGWCC 1 cut(s) 1241
XapI RAATTY 5 cut(s) 203, 257, 506, 682, 755
XceI RCATGY 5 cut(s) 293, 561, 1072, 1165, 1186
XspI CTAG 3 cut(s) 149, 933, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.