RLG00000026727

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000663
Physical Location & Seq
Reverse (-)
5348840 .. 5351624
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026727

Sequence Viewer

Length: 1515 bp
ATGAGTCTATTTGGGAGATTAAGGATGGTGGTTTTACTGGCGGCGATTATGGTGGTGATAATGGTGAAGGCTTCGCAGGACTATGGGGATGCATTGACGAAGAGCATATTGTTCTTTGAGGGCCAGAGGTCAGGGAAGCTACCCTCTCGTCAGAGAATGACATGGAGGAAAGATTCTGCACTTCAGGATGGACTTCAGATTGGTGTTGATTTGGTAGGGGGTTACTACGATGCCGGTGACAATGTGAAGTTCAACTTTCCGATGGCTTTCTCGACAACAATGCTGGCATGGAGCGTGTTAGAGTTCGGGAACGACATGGGTGCAGACCTACCCCGTGCACTTGATGCCATTCGATGGGGAACGGACTACTTCCTCAAAGCCACAAGCATTCCTGACTTTGTGTTTGCCCAAGTTGGTGAACCTTACGGCGACCACAATTGTTGGGAGAGGCCTGAGGACATGGACACCCCAAGAAACCCCTATGCAGTTAGCAAACAGTTTCCAGGTTCTGAAGTCTCGGCAGAGATTGCAGCCGCCCTAGCAGCCTCTTCCATGGTCTTTAAGCACGTTGATTTGGAATACTCTGCAAGGCTTCTCAAAAGGGCTAGAATGGTGTTTGAGTTTGCAGAAACATACCGGGGCTCCTATAATGATGCTCTTGGACCTTGGGTTTGTCCATTTTACTGTGATTTTAGTGGCTATGAGGACGAATTGATTTGGGGAGCAACATGGTTGTTTAAGGCAACTACGGACCCTACATACTCGAACTATGTCGAAGAAAACGTACACAAGCTGGAAAAATCTGTCATGAGAAATGTAAATGGTTTACCATATGCTTCTGTAGGTGGAAGCTTTGCCGAATTTGGATGGGATTCAAAGCACGCAGGCATCAATGTACTGGCTTCCAAGTTGTTCATGTCTACCATCGATATCGGTTCTAGTCCTTTCATTCACAATTCCGACAAGTTCATATGCAGTTTGTTACCTGAATCACCAACCATATTAGTCTCATATTCTCCAGGTGGGCTTTTATTTAAACCTGGAGGAAGTAATCTGCAGCATGCAACAACTCTGTCATTTCTCTTGGTTGTTTATTCTCGTTATCTGAAGCAAGCCAATAGAGTAGTTTACTGTAACAATGTTGTTGCCAGTCCGGCTAGACTTGTTCAAGTTGCAAAAGGCCAGGTGGATTACATACTAGGAAGTAACCCATTGAACATGTCATATATGGTCGGGTATGGCAAAAAGTTTCCTCAGAGAATTCATCATCGGGGGTCATCGTTACCGTCGGTTGGTCAACACCCTCAACAGATTGGTTCCAAAGACGGAAGCGTTTACTTTGACAGCAGTAACCCAAATCCTAATATGCTAACTGGGGCTGTTGTTGGAGGACCAGATATAAAGGATTCATATGAAGATTCTCGAGTGGATTTTGTGCAGTCCGAGCCAACCACATACATCAATGCACCATTTGTAGGCGTCTTGGCTTACTTCAAATCGCATCCATGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

505

Amino Acids

55.83

Weight (kDa)

6.08

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 495 2.4e-150 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 354
AccI GTMKAC 1 cut(s) 920
AciI CCGC 2 cut(s) 41, 534
AcsI RAATTY 2 cut(s) 860, 1260
AcuI CTGAAG 4 cut(s) 167, 179, 531, 1127
AcyI GRCGYC 1 cut(s) 1479
AfaI GTAC 2 cut(s) 786, 897
AfiI CCNNNNNNNGG 3 cut(s) 354, 1292, 1475
AflIII ACRYGT 1 cut(s) 1218
AgsI TTSAA 5 cut(s) 253, 876, 1169, 1216, 1495
AjnI CCWGG 4 cut(s) 502, 1018, 1039, 1182
AluBI AGCT 3 cut(s) 139, 793, 852
AluI AGCT 3 cut(s) 139, 793, 852
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 520, 1012
Alw44I GTGCAC 1 cut(s) 336
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 1422
AoxI GGCC 3 cut(s) 121, 449, 1180
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 3 cut(s) 530, 542, 1057
ApoI RAATTY 2 cut(s) 860, 1260
ArsI GACNNNNNNTTYG 4 cut(s) 386, 418, 654, 686
AspS9I GGNCC 5 cut(s) 121, 662, 751, 1391, 1509
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 5 cut(s) 67, 76, 248, 428, 984
AvaI CYCGRG 1 cut(s) 1422
AvaII GGWCC 4 cut(s) 662, 751, 1391, 1509
AxyI CCTNAGG 1 cut(s) 453
BaeGI GKGCMC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 644
BarI GAAGNNNNNNTAC 2 cut(s) 768, 800
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 3 cut(s) 542, 554, 1069
BccI CCATC 6 cut(s) 19, 182, 256, 348, 861, 932
BceAI ACGGC 1 cut(s) 442
BcgI CGANNNNNNTGC 4 cut(s) 262, 296, 302, 336
BciT130I CCWGG 4 cut(s) 504, 1020, 1041, 1184
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 520, 1012
BfaI CTAG 5 cut(s) 539, 606, 939, 1158, 1199
BfmI CTRYAG 2 cut(s) 840, 1055
BglI GCCNNNNNGGC 1 cut(s) 1154
BisI GCNGC 5 cut(s) 42, 531, 534, 543, 1058
BlsI GCNGC 5 cut(s) 43, 532, 535, 544, 1059
Bme1390I CCNGG 5 cut(s) 504, 638, 1020, 1041, 1184
Bme18I GGWCC 4 cut(s) 662, 751, 1391, 1509
BmeT110I CYCGRG 1 cut(s) 1422
BmgT120I GGNCC 5 cut(s) 121, 662, 751, 1391, 1509
BmiI GGNNCC 3 cut(s) 643, 753, 1318
BmrFI CCNGG 5 cut(s) 504, 638, 1020, 1041, 1184
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1510
BmuI ACTGGG 1 cut(s) 1383
BpmI CTGGAG 2 cut(s) 1002, 1062
BpuMI CCSGG 1 cut(s) 638
Bsa29I ATCGAT 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 1479
BsaJI CCNNGG 4 cut(s) 552, 637, 665, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 1292, 1475
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 4 cut(s) 42, 903, 1149, 1378
Bse21I CCTNAGG 1 cut(s) 453
BseBI CCWGG 4 cut(s) 504, 1020, 1041, 1184
BseCI ATCGAT 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 552, 637, 665, 1505
BseGI GGATG 5 cut(s) 30, 94, 193, 872, 1501
BseLI CCNNNNNNNGG 3 cut(s) 354, 1292, 1475
BseMII CTCAG 2 cut(s) 444, 1268
BseNI ACTGG 4 cut(s) 42, 903, 1149, 1378
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 3 cut(s) 542, 554, 1069
BsgI GTGCAG 3 cut(s) 162, 342, 1457
BshFI GGCC 3 cut(s) 123, 451, 1182
BshVI ATCGAT 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 3 cut(s) 234, 637, 1154
BslI CCNNNNNNNGG 3 cut(s) 354, 1292, 1475
BsmAI GTCTC 2 cut(s) 520, 1012
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 3 cut(s) 123, 451, 1182
BsoBI CYCGRG 1 cut(s) 1422
Bsp1286I GDGCHC 2 cut(s) 340, 644
Bsp19I CCATGG 2 cut(s) 552, 1505
BspACI CCGC 2 cut(s) 41, 534
BspANI GGCC 3 cut(s) 123, 451, 1182
BspCNI CTCAG 2 cut(s) 445, 1267
BspDI ATCGAT 1 cut(s) 927
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 3 cut(s) 643, 753, 1318
BspMAI CTGCAG 1 cut(s) 1059
BspQI GCTCTTC 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 4 cut(s) 42, 903, 1149, 1378
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 4 cut(s) 552, 637, 665, 1505
BssNI GRCGYC 1 cut(s) 1479
BssT1I CCWWGG 3 cut(s) 552, 665, 1505
Bst2UI CCWGG 4 cut(s) 504, 1020, 1041, 1184
Bst4CI ACNGT 4 cut(s) 498, 686, 1133, 1287
Bst6I CTCTTC 2 cut(s) 95, 553
BstACI GRCGYC 1 cut(s) 1479
BstAPI GCANNNNNTGC 2 cut(s) 344, 527
BstC8I GCNNGC 5 cut(s) 285, 882, 886, 1062, 1113
BstDEI CTNAG 2 cut(s) 453, 1254
BstDSI CCRYGG 2 cut(s) 552, 1505
BstF5I GGATG 5 cut(s) 30, 94, 193, 872, 1501
BstMAI GTCTC 2 cut(s) 520, 1012
BstMWI GCNNNNNNNGC 6 cut(s) 344, 527, 539, 542, 1154, 1444
BstNI CCWGG 4 cut(s) 504, 1020, 1041, 1184
BstNSI RCATGY 2 cut(s) 1064, 1222
BstSCI CCNGG 5 cut(s) 502, 636, 1018, 1039, 1182
BstSFI CTRYAG 2 cut(s) 840, 1055
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 542, 554, 1069
Bsu15I ATCGAT 1 cut(s) 927
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 3 cut(s) 123, 451, 1182
BsuTUI ATCGAT 1 cut(s) 927
BtgI CCRYGG 2 cut(s) 552, 1505
BtsCI GGATG 5 cut(s) 30, 94, 193, 872, 1501
Cac8I GCNNGC 5 cut(s) 285, 882, 886, 1062, 1113
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 807
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 5 cut(s) 121, 662, 751, 1391, 1509
ClaI ATCGAT 1 cut(s) 927
CseI GACGC 1 cut(s) 1468
Csp6I GTAC 2 cut(s) 785, 896
CspCI CAANNNNNGTGG 2 cut(s) 422, 457
CviQI GTAC 2 cut(s) 785, 896
DdeI CTNAG 2 cut(s) 453, 1254
DraI TTTAAA 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 95, 553
EarI CTCTTC 2 cut(s) 95, 553
Eco130I CCWWGG 3 cut(s) 552, 665, 1505
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 644
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 4 cut(s) 662, 751, 1391, 1509
Eco57I CTGAAG 4 cut(s) 167, 179, 531, 1127
Eco81I CCTNAGG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 1422
EcoRI GAATTC 1 cut(s) 1260
EcoRII CCWGG 4 cut(s) 502, 1018, 1039, 1182
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 552, 665, 1505
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 3 cut(s) 552, 665, 1505
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FauNDI CATATG 3 cut(s) 832, 971, 1411
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 5 cut(s) 42, 531, 534, 543, 1058
FokI GGATG 5 cut(s) 37, 101, 200, 879, 1488
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 5 cut(s) 42, 531, 534, 543, 1058
FspBI CTAG 5 cut(s) 539, 606, 939, 1158, 1199
GluI GCNGC 5 cut(s) 42, 531, 534, 543, 1058
GsuI CTGGAG 2 cut(s) 1002, 1062
HaeIII GGCC 3 cut(s) 123, 451, 1182
HapII CCGG 3 cut(s) 234, 637, 1154
HgaI GACGC 1 cut(s) 1468
Hin1I GRCGYC 1 cut(s) 1479
HincII GTYRAC 1 cut(s) 1298
HindII GTYRAC 1 cut(s) 1298
HindIII AAGCTT 1 cut(s) 850
HinfI GANTC 6 cut(s) 4, 173, 872, 989, 1406, 1418
HpaII CCGG 3 cut(s) 234, 637, 1154
HphI GGTGA 5 cut(s) 67, 76, 248, 428, 984
Hpy166II GTNNAC 8 cut(s) 338, 419, 787, 827, 921, 1129, 1298, 1336
Hpy188I TCNGA 8 cut(s) 153, 198, 261, 511, 961, 1107, 1257, 1444
Hpy188III TCNNGA 6 cut(s) 185, 271, 307, 392, 808, 1422
Hpy8I GTNNAC 8 cut(s) 338, 419, 787, 827, 921, 1129, 1298, 1336
Hpy99I CGWCG 1 cut(s) 1291
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 4 cut(s) 498, 686, 1133, 1287
HpyCH4IV ACGT 2 cut(s) 567, 783
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 527, 539, 542, 1154, 1444
HpyF3I CTNAG 2 cut(s) 453, 1254
HpySE526I ACGT 2 cut(s) 567, 783
Hsp92I GRCGYC 1 cut(s) 1479
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 3 cut(s) 291, 647, 722
Lsp1109I GCAGC 3 cut(s) 542, 554, 1069
LweI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1510
MaeI CTAG 5 cut(s) 539, 606, 939, 1158, 1199
MaeII ACGT 2 cut(s) 567, 783
MaeIII GTNAC 7 cut(s) 221, 236, 981, 1133, 1205, 1281, 1349
MboII GAAGA 4 cut(s) 112, 540, 788, 1427
MfeI CAATTG 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 340, 644
MluCI AATT 5 cut(s) 436, 710, 860, 955, 1260
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 984, 1366
Mph1103I ATGCAT 1 cut(s) 94
MseI TTAA 4 cut(s) 20, 561, 738, 1035
MspI CCGG 3 cut(s) 234, 637, 1154
MspR9I CCNGG 5 cut(s) 504, 638, 1020, 1041, 1184
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 4 cut(s) 504, 1020, 1041, 1184
MwoI GCNNNNNNNGC 6 cut(s) 344, 527, 539, 542, 1154, 1444
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 2 cut(s) 552, 1505
NdeI CATATG 3 cut(s) 832, 971, 1411
NlaIV GGNNCC 3 cut(s) 643, 753, 1318
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 2 cut(s) 1064, 1222
PaeI GCATGC 1 cut(s) 1064
PaeR7I CTCGAG 1 cut(s) 1422
PagI TCATGA 1 cut(s) 807
PceI AGGCCT 1 cut(s) 451
PciI ACATGT 1 cut(s) 1218
PciSI GCTCTTC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 780
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 5 cut(s) 173, 872, 989, 1406, 1418
PflMI CCANNNNNTGG 1 cut(s) 354
PkrI GCNGC 5 cut(s) 43, 532, 535, 544, 1059
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 1218
Psp6I CCWGG 4 cut(s) 502, 1018, 1039, 1182
PspGI CCWGG 4 cut(s) 502, 1018, 1039, 1182
PspN4I GGNNCC 3 cut(s) 643, 753, 1318
PspPI GGNCC 5 cut(s) 121, 662, 751, 1391, 1509
PstI CTGCAG 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 509
RsaI GTAC 2 cut(s) 786, 897
RsaNI GTAC 2 cut(s) 785, 896
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 20, 561, 738, 1035
SatI GCNGC 5 cut(s) 42, 531, 534, 543, 1058
Sau96I GGNCC 5 cut(s) 121, 662, 751, 1391, 1509
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 5 cut(s) 504, 638, 1020, 1041, 1184
SduI GDGCHC 2 cut(s) 340, 644
SfaNI GCATC 6 cut(s) 79, 220, 334, 643, 897, 1510
SfcI CTRYAG 2 cut(s) 840, 1055
Sfr274I CTCGAG 1 cut(s) 1422
SinI GGWCC 4 cut(s) 662, 751, 1391, 1509
SlaI CTCGAG 1 cut(s) 1422
SmlI CTYRAG 1 cut(s) 1422
SmoI CTYRAG 1 cut(s) 1422
SphI GCATGC 1 cut(s) 1064
Sse9I AATT 5 cut(s) 436, 710, 860, 955, 1260
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 2 cut(s) 41, 534
SspMI CTAG 5 cut(s) 539, 606, 939, 1158, 1199
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 5 cut(s) 502, 636, 1018, 1039, 1182
StyI CCWWGG 3 cut(s) 552, 665, 1505
TaaI ACNGT 4 cut(s) 498, 686, 1133, 1287
TaiI ACGT 2 cut(s) 570, 786
TaqI TCGA 6 cut(s) 272, 352, 764, 774, 927, 1423
TasI AATT 5 cut(s) 436, 710, 860, 955, 1260
TatI WGTACW 1 cut(s) 895
TauI GCSGC 2 cut(s) 44, 536
TfiI GAWTC 5 cut(s) 173, 872, 989, 1406, 1418
Tru1I TTAA 4 cut(s) 20, 561, 738, 1035
Tru9I TTAA 4 cut(s) 20, 561, 738, 1035
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 3 cut(s) 530, 542, 1057
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 6 cut(s) 904, 937, 958, 1253, 1398, 1428
TspGWI ACGGA 3 cut(s) 377, 764, 1341
Van91I CCANNNNNTGG 1 cut(s) 354
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 4 cut(s) 662, 751, 1391, 1509
XapI RAATTY 2 cut(s) 860, 1260
XceI RCATGY 2 cut(s) 1064, 1222
XhoI CTCGAG 1 cut(s) 1422
XmiI GTMKAC 1 cut(s) 920
XspI CTAG 5 cut(s) 539, 606, 939, 1158, 1199
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.