Rmu_sc0004947.1_g000006

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004947.1
OG0000663
Physical Location & Seq
Forward (+)
25137 .. 27000
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004947.1_g000006.1.cds

Sequence Viewer

Length: 1035 bp
atggatcttgtagggggttactatgatgcaggagacaatgtgaagttcaactttcccatggccttcacaacaacaatgttgtcgtggagcgtgctagagttcggaaatctaatgggatcggaccttcaacatgctatggaagctataagatgggggactgattattttctgaaagccacaaacgttcctgattcggttgtgggtgtagttggcgatcccattggtgaccacagttgttctcaaaggcctgaggacatggacaccccaagaacctcttatgtagtcaccaaagaaaagccaggttcagaagtctcagccgaaattgcagctgcatttgcagcttcttccttggtgttcaaatattcagacaataactactctactttgcttcttaaccgatcaatgcaggtctttgaatttgcagataagtatagggggtcttacaatgacagtattggggaaggagcatgccccttttactgtgattttagtggctacatggatgaattgctctggggagcttcttggctatacaaggcaaccggagcagctgattattggaattatgtgagagagaacatagacaaaatggagtccactgtaataagaaatttcaatggggatcctgtttcatacataaatggtgctgtcactgaatttggatgggattcaaagcattccgggattaacgttgattatatactaggaagcaacccattaggcatgtcatacatggtcggattcgggccacagtttcctcagaaaatacatcaccgcggttctgttctaccatcccttgatcaacacccacaacacatcgattgccgcggaggagacgtgtattggcaaagccaaggtcctaatctgaatttgctaacaggtgcaattgtgggaggtcctgctgaaaatgatacgttccaagattctcgatataatgttccacaatcagagccaactacatacatcaacgctccttttgtgggagttttggcttactttaaatcactatttccccaactgaatccaagaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

344

Amino Acids

38.19

Weight (kDa)

4.72

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccII CGCG 2 cut(s) 777, 828
AciI CCGC 4 cut(s) 775, 777, 826, 828
AclI AACGTT 2 cut(s) 183, 690
AclWI GGATC 5 cut(s) 12, 124, 209, 617, 630
AcsI RAATTY 4 cut(s) 416, 610, 656, 868
AfiI CCNNNNNNNGG 1 cut(s) 980
AflIII ACRYGT 1 cut(s) 837
AgsI TTSAA 6 cut(s) 49, 128, 358, 416, 616, 672
AjiI CACGTC 1 cut(s) 838
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 5 cut(s) 143, 329, 341, 521, 551
AluI AGCT 5 cut(s) 143, 329, 341, 521, 551
Alw26I GTCTC 3 cut(s) 27, 316, 828
AlwI GGATC 5 cut(s) 12, 124, 209, 617, 630
AoxI GGCC 3 cut(s) 60, 245, 746
ApeKI GCWGC 4 cut(s) 326, 329, 338, 548
ApoI RAATTY 4 cut(s) 416, 610, 656, 868
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
AspS9I GGNCC 4 cut(s) 121, 746, 857, 896
AsuC2I CCSGG 1 cut(s) 682
AsuHPI GGTGA 3 cut(s) 236, 277, 764
AvaII GGWCC 3 cut(s) 121, 857, 896
AxyI CCTNAGG 1 cut(s) 249
BamHI GGATCC 1 cut(s) 622
BbvI GCAGC 4 cut(s) 316, 338, 350, 560
BccI CCATC 3 cut(s) 144, 657, 799
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 682
BcoDI GTCTC 3 cut(s) 27, 316, 828
BfaI CTAG 2 cut(s) 95, 704
BfuAI ACCTGC 1 cut(s) 397
BisI GCNGC 5 cut(s) 327, 330, 339, 549, 826
BlsI GCNGC 5 cut(s) 328, 331, 340, 550, 827
Bme1390I CCNGG 2 cut(s) 300, 682
Bme18I GGWCC 3 cut(s) 121, 857, 896
BmgBI CACGTC 1 cut(s) 838
BmgT120I GGNCC 4 cut(s) 121, 746, 857, 896
BmiI GGNNCC 1 cut(s) 624
BmrFI CCNGG 2 cut(s) 300, 682
BmsI GCATC 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 682
Bsa29I ATCGAT 1 cut(s) 819
BsaJI CCNNGG 5 cut(s) 57, 348, 775, 826, 853
BsaWI WCCGGW 1 cut(s) 542
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 822, 852
Bsc4I CCNNNNNNNGG 1 cut(s) 980
Bse21I CCTNAGG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 5 cut(s) 57, 348, 775, 826, 853
BseGI GGATG 3 cut(s) 508, 668, 791
BseLI CCNNNNNNNGG 1 cut(s) 980
BseMII CTCAG 3 cut(s) 240, 327, 773
BseRI GAGGAG 1 cut(s) 846
BseXI GCAGC 4 cut(s) 316, 338, 350, 560
Bsh1236I CGCG 2 cut(s) 777, 828
BshFI GGCC 3 cut(s) 62, 247, 748
BshVI ATCGAT 1 cut(s) 819
BsiSI CCGG 2 cut(s) 543, 681
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 980
BsmAI GTCTC 3 cut(s) 27, 316, 828
BsmBI CGTCTC 1 cut(s) 828
BsmFI GGGAC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 3 cut(s) 62, 247, 748
Bsp143I GATC 6 cut(s) 4, 116, 214, 398, 622, 799
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 4 cut(s) 775, 777, 826, 828
BspANI GGCC 3 cut(s) 62, 247, 748
BspCNI CTCAG 3 cut(s) 241, 326, 772
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 2 cut(s) 777, 828
BspLI GGNNCC 1 cut(s) 624
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 5 cut(s) 12, 124, 209, 617, 630
BssECI CCNNGG 5 cut(s) 57, 348, 775, 826, 853
BssMI GATC 6 cut(s) 4, 116, 214, 398, 622, 799
BssT1I CCWWGG 3 cut(s) 57, 348, 853
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 5 cut(s) 233, 452, 482, 601, 753
BstC8I GCNNGC 2 cut(s) 92, 469
BstDEI CTNAG 3 cut(s) 249, 313, 759
BstDSI CCRYGG 3 cut(s) 57, 775, 826
BstEII GGTNACC 1 cut(s) 224
BstF5I GGATG 3 cut(s) 508, 668, 791
BstFNI CGCG 2 cut(s) 777, 828
BstKTI GATC 6 cut(s) 7, 119, 217, 401, 625, 802
BstMAI GTCTC 3 cut(s) 27, 316, 828
BstMBI GATC 6 cut(s) 4, 116, 214, 398, 622, 799
BstMWI GCNNNNNNNGC 5 cut(s) 140, 323, 335, 338, 545
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 3 cut(s) 134, 471, 727
BstPI GGTNACC 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 298, 680
BstUI CGCG 2 cut(s) 777, 828
BstV1I GCAGC 4 cut(s) 316, 338, 350, 560
BstX2I RGATCY 2 cut(s) 4, 622
BstYI RGATCY 2 cut(s) 4, 622
Bsu15I ATCGAT 1 cut(s) 819
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 3 cut(s) 62, 247, 748
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 3 cut(s) 57, 775, 826
BtrI CACGTC 1 cut(s) 838
BtsCI GGATG 3 cut(s) 508, 668, 791
BtsIMutI CAGTG 2 cut(s) 597, 651
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 92, 469
Cfr13I GGNCC 4 cut(s) 121, 746, 857, 896
Cfr42I CCGCGG 2 cut(s) 778, 829
ClaI ATCGAT 1 cut(s) 819
CviAII CATG 7 cut(s) 58, 131, 256, 468, 499, 724, 733
DdeI CTNAG 3 cut(s) 249, 313, 759
DpnI GATC 6 cut(s) 6, 118, 216, 400, 624, 801
DpnII GATC 6 cut(s) 4, 116, 214, 398, 622, 799
DraI TTTAAA 1 cut(s) 1000
Eco130I CCWWGG 3 cut(s) 57, 348, 853
Eco147I AGGCCT 1 cut(s) 247
Eco47I GGWCC 3 cut(s) 121, 857, 896
Eco81I CCTNAGG 1 cut(s) 249
Eco91I GGTNACC 1 cut(s) 224
EcoO109I RGGNCCY 2 cut(s) 857, 896
EcoO65I GGTNACC 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 3 cut(s) 57, 348, 853
ErhI CCWWGG 3 cut(s) 57, 348, 853
Esp3I CGTCTC 1 cut(s) 828
FaeI CATG 7 cut(s) 61, 134, 259, 471, 502, 727, 736
FalI AAGNNNNNCTT 4 cut(s) 35, 67, 259, 291
FaqI GGGAC 1 cut(s) 169
FatI CATG 7 cut(s) 57, 130, 255, 467, 498, 723, 732
FbaI TGATCA 1 cut(s) 799
Fnu4HI GCNGC 5 cut(s) 327, 330, 339, 549, 826
FokI GGATG 3 cut(s) 515, 675, 778
Fsp4HI GCNGC 5 cut(s) 327, 330, 339, 549, 826
FspBI CTAG 2 cut(s) 95, 704
GluI GCNGC 5 cut(s) 327, 330, 339, 549, 826
HaeIII GGCC 3 cut(s) 62, 247, 748
HapII CCGG 2 cut(s) 543, 681
Hin1II CATG 7 cut(s) 61, 134, 259, 471, 502, 727, 736
HinfI GANTC 6 cut(s) 191, 593, 668, 741, 923, 1021
HpaII CCGG 2 cut(s) 543, 681
HphI GGTGA 3 cut(s) 236, 277, 764
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 9 cut(s) 104, 121, 171, 307, 367, 740, 762, 867, 949
Hpy188III TCNNGA 2 cut(s) 188, 927
Hpy8I GTNNAC 1 cut(s) 597
HpyAV CCTTC 3 cut(s) 73, 134, 455
HpyCH4III ACNGT 5 cut(s) 233, 452, 482, 601, 753
HpyCH4IV ACGT 4 cut(s) 183, 690, 837, 914
HpyCH4V TGCA 7 cut(s) 29, 326, 332, 338, 406, 422, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 323, 335, 338, 545
HpyF3I CTNAG 3 cut(s) 249, 313, 759
HpySE526I ACGT 4 cut(s) 183, 690, 837, 914
Hsp92II CATG 7 cut(s) 61, 134, 259, 471, 502, 727, 736
Ksp22I TGATCA 1 cut(s) 799
KspI CCGCGG 2 cut(s) 778, 829
Kzo9I GATC 6 cut(s) 4, 116, 214, 398, 622, 799
LmnI GCTCC 5 cut(s) 87, 464, 518, 545, 976
Lsp1109I GCAGC 4 cut(s) 316, 338, 350, 560
LweI GCATC 1 cut(s) 16
MaeI CTAG 2 cut(s) 95, 704
MaeII ACGT 4 cut(s) 183, 690, 837, 914
MaeIII GTNAC 4 cut(s) 17, 224, 283, 649
MalI GATC 6 cut(s) 6, 118, 216, 400, 624, 801
MboI GATC 6 cut(s) 4, 116, 214, 398, 622, 799
MboII GAAGA 1 cut(s) 336
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 2 cut(s) 4, 622
MluCI AATT 8 cut(s) 321, 416, 506, 562, 610, 656, 868, 885
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 718
MnlI CCTC 5 cut(s) 244, 283, 768, 824, 887
MseI TTAA 3 cut(s) 393, 687, 999
MspA1I CMGCKG 4 cut(s) 329, 551, 777, 828
MspI CCGG 2 cut(s) 543, 681
MspR9I CCNGG 2 cut(s) 300, 682
MunI CAATTG 1 cut(s) 885
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 2 cut(s) 777, 828
MwoI GCNNNNNNNGC 5 cut(s) 140, 323, 335, 338, 545
NciI CCSGG 1 cut(s) 682
NcoI CCATGG 1 cut(s) 57
NdeII GATC 6 cut(s) 4, 116, 214, 398, 622, 799
NlaIII CATG 7 cut(s) 61, 134, 259, 471, 502, 727, 736
NlaIV GGNNCC 1 cut(s) 624
NmuCI GTSAC 3 cut(s) 224, 283, 649
NspI RCATGY 3 cut(s) 134, 471, 727
PaeI GCATGC 1 cut(s) 471
PceI AGGCCT 1 cut(s) 247
PctI GAATGC 1 cut(s) 676
PfeI GAWTC 5 cut(s) 191, 668, 741, 923, 1021
PfoI TCCNGGA 1 cut(s) 680
PkrI GCNGC 5 cut(s) 328, 331, 340, 550, 827
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PpuMI RGGWCCY 2 cut(s) 857, 896
Psp1406I AACGTT 2 cut(s) 183, 690
Psp5II RGGWCCY 2 cut(s) 857, 896
Psp6I CCWGG 1 cut(s) 298
PspEI GGTNACC 1 cut(s) 224
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 4 cut(s) 121, 746, 857, 896
PspPPI RGGWCCY 2 cut(s) 857, 896
PsuI RGATCY 2 cut(s) 4, 622
PvuII CAGCTG 2 cut(s) 329, 551
SacII CCGCGG 2 cut(s) 778, 829
SaqAI TTAA 3 cut(s) 393, 687, 999
SatI GCNGC 5 cut(s) 327, 330, 339, 549, 826
Sau3AI GATC 6 cut(s) 4, 116, 214, 398, 622, 799
Sau96I GGNCC 4 cut(s) 121, 746, 857, 896
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 2 cut(s) 300, 682
SfaNI GCATC 1 cut(s) 16
Sfr303I CCGCGG 2 cut(s) 778, 829
SgrBI CCGCGG 2 cut(s) 778, 829
SinI GGWCC 3 cut(s) 121, 857, 896
SphI GCATGC 1 cut(s) 471
Sse9I AATT 8 cut(s) 321, 416, 506, 562, 610, 656, 868, 885
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 4 cut(s) 775, 777, 826, 828
SspI AATATT 1 cut(s) 362
SspMI CTAG 2 cut(s) 95, 704
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 298, 680
StyI CCWWGG 3 cut(s) 57, 348, 853
TaaI ACNGT 5 cut(s) 233, 452, 482, 601, 753
TaiI ACGT 4 cut(s) 186, 693, 840, 917
TaqI TCGA 2 cut(s) 819, 928
TasI AATT 8 cut(s) 321, 416, 506, 562, 610, 656, 868, 885
TauI GCSGC 1 cut(s) 828
TfiI GAWTC 5 cut(s) 191, 668, 741, 923, 1021
Tru1I TTAA 3 cut(s) 393, 687, 999
Tru9I TTAA 3 cut(s) 393, 687, 999
TscAI CASTG 2 cut(s) 604, 658
TseFI GTSAC 3 cut(s) 224, 283, 649
TseI GCWGC 4 cut(s) 326, 329, 338, 548
Tsp45I GTSAC 3 cut(s) 224, 283, 649
TspDTI ATGAA 2 cut(s) 519, 621
TspRI CASTG 2 cut(s) 604, 658
VpaK11BI GGWCC 3 cut(s) 121, 857, 896
XapI RAATTY 4 cut(s) 416, 610, 656, 868
XceI RCATGY 3 cut(s) 134, 471, 727
XspI CTAG 2 cut(s) 95, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.