Rorug01G0384200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000663
Physical Location & Seq
Forward (+)
49617591 .. 49620169
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0384200.1

Sequence Viewer

Length: 339 bp
ATGGTGAAGGGACGTCAAGGAGAGCGAGTCAGACTCTACGTCAGGGGAACTGTCCTCGGCTTCAAGAGGTCCAAGTCGAACCAGTACCCAAACACCTCACTGATTCAGATCGAGGGAGTGAACACCAAGGAGGAAGTTGGGTGGTACGCCGGAAAGCGATTGGCTTATATCTACAAGGCTAAGGTGAAGCAGAACGGGAGCCACTACCGCTGCATTTGGGGAAAGGTTGCGAGGCCTCACGGTAACACCGGTGTTGTTCGGGCTAAGTTCACCTCCAATTTGCCCCCCAAGTCCATGGGTGCTCGAGTGAGGGTATTCATGTATCCCAGCAATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

112

Amino Acids

12.73

Weight (kDa)

10.77

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35Ae PF01247 12 - 106 2e-43 Ribosomal protein L35Ae
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 16
AciI CCGC 1 cut(s) 208
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 2 cut(s) 86, 146
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 1 cut(s) 64
AhdI GACNNNNNGTC 1 cut(s) 38
Alw21I GWGCWC 1 cut(s) 304
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 210
AsiGI ACCGGT 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 69
AsuHPI GGTGA 3 cut(s) 16, 196, 262
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 197
BciVI GTATCC 1 cut(s) 333
BfuI GTATCC 1 cut(s) 333
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 69
BmeRI GACNNNNNGTC 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 1 cut(s) 200
BplI GAGNNNNNCTC 2 cut(s) 18, 50
Bpu10I CCTNAGC 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 13
BsaJI CCNNGG 3 cut(s) 55, 126, 294
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 82
Bse8I GATNNNNATC 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 55, 126, 294
BseJI GATNNNNATC 1 cut(s) 107
BseNI ACTGG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 197
BseYI CCCAGC 1 cut(s) 326
BshFI GGCC 1 cut(s) 235
BshTI ACCGGT 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 304
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 2 cut(s) 150, 249
BslFI GGGAC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 1 cut(s) 208
BspANI GGCC 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 55, 126, 294
BssMI GATC 1 cut(s) 108
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 126, 294
Bst4CI ACNGT 2 cut(s) 52, 242
BstACI GRCGYC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 180, 264
BstDSI CCRYGG 1 cut(s) 294
BstKTI GATC 1 cut(s) 111
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 295
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 98
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 69
Csp6I GTAC 2 cut(s) 85, 145
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 2 cut(s) 295, 319
CviJI RGCY 6 cut(s) 60, 164, 179, 201, 235, 263
CviKI_1 RGCY 6 cut(s) 60, 164, 179, 201, 235, 263
CviQI GTAC 2 cut(s) 85, 145
DdeI CTNAG 2 cut(s) 180, 264
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
DriI GACNNNNNGTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 126, 294
Eco147I AGGCCT 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 303
EcoT14I CCWWGG 2 cut(s) 126, 294
ErhI CCWWGG 2 cut(s) 126, 294
FaeI CATG 2 cut(s) 298, 322
FaiI YATR 5 cut(s) 168, 296, 320, 335, 337
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 294, 318
Fnu4HI GCNGC 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
GsaI CCCAGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 235
HapII CCGG 2 cut(s) 150, 249
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 2 cut(s) 298, 322
HinfI GANTC 3 cut(s) 27, 33, 103
HpaII CCGG 2 cut(s) 150, 249
HphI GGTGA 3 cut(s) 16, 196, 262
Hpy166II GTNNAC 2 cut(s) 121, 270
Hpy188I TCNGA 2 cut(s) 32, 108
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 2 cut(s) 121, 270
HpyCH4III ACNGT 2 cut(s) 52, 242
HpyCH4IV ACGT 2 cut(s) 13, 39
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 2 cut(s) 180, 264
HpySE526I ACGT 2 cut(s) 13, 39
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 2 cut(s) 298, 322
Kzo9I GATC 1 cut(s) 108
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 4 cut(s) 28, 95, 163, 262
Lsp1109I GCAGC 1 cut(s) 197
MaeII ACGT 2 cut(s) 13, 39
MaeIII GTNAC 1 cut(s) 242
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 1 cut(s) 277
MlyI GAGTC 2 cut(s) 27, 36
MnlI CCTC 9 cut(s) 60, 65, 106, 106, 124, 225, 246, 283, 303
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 2 cut(s) 150, 249
MwoI GCNNNNNNNGC 1 cut(s) 207
NcoI CCATGG 1 cut(s) 294
NdeII GATC 1 cut(s) 108
NlaIII CATG 2 cut(s) 298, 322
NlaIV GGNNCC 1 cut(s) 200
NmeAIII GCCGAG 1 cut(s) 36
PaeR7I CTCGAG 1 cut(s) 303
PceI AGGCCT 1 cut(s) 235
PfeI GAWTC 1 cut(s) 103
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 1 cut(s) 212
PleI GAGTC 2 cut(s) 27, 35
PpsI GAGTC 2 cut(s) 27, 35
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 1 cut(s) 69
PspXI VCTCGAGB 1 cut(s) 303
RsaI GTAC 2 cut(s) 86, 146
RsaNI GTAC 2 cut(s) 85, 145
SatI GCNGC 1 cut(s) 211
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 1 cut(s) 69
SchI GAGTC 2 cut(s) 27, 36
SduI GDGCHC 1 cut(s) 304
SetI ASST 7 cut(s) 16, 42, 71, 98, 186, 228, 275
Sfr274I CTCGAG 1 cut(s) 303
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 69
SlaI CTCGAG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 1 cut(s) 277
SseBI AGGCCT 1 cut(s) 235
SsiI CCGC 1 cut(s) 208
StuI AGGCCT 1 cut(s) 235
StyI CCWWGG 2 cut(s) 126, 294
TaaI ACNGT 2 cut(s) 52, 242
TaiI ACGT 2 cut(s) 16, 42
TaqI TCGA 3 cut(s) 77, 111, 304
TasI AATT 1 cut(s) 277
TfiI GAWTC 1 cut(s) 103
TscAI CASTG 1 cut(s) 105
TseI GCWGC 1 cut(s) 210
TspDTI ATGAA 1 cut(s) 307
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 69
XhoI CTCGAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.